We narrowed to 18,867 results for: IRE
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Plasmid#186406PurposeDestination vector for ascidian transgenesis by electroporation of a Gateway-cloned fusion of a gene of interest with a C-ter HA tag under control of the pFOG ectodermal regulatory sequence.DepositorTypeEmpty backboneUseGateway destination vectorTagsExpressionMutationPromoterAvailable sinceAug. 9, 2022AvailabilityAcademic Institutions and Nonprofits only
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Lentiguide-Blast-eNMU710
Plasmid#172658PurposeExpressing paired pegRNAs from human U6 and H1 promoters to make 710-bp deletion on e-NMU locusDepositorInsertpegRNA-eNMUA/pegRNA-eNMUB
UseCRISPRTagsExpressionMutationPromoterAvailable sinceDec. 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
PB-HD252
Plasmid#172656PurposeExpressing paired pegRNAs from human U6 and H1 promoters to make 252-bp deletion on HPRT1 geneDepositorInsertpegRNA-HD252A/pegRNA-HD252B
UseCRISPRTagsExpressionMutationPromoterAvailable sinceOct. 22, 2021AvailabilityAcademic Institutions and Nonprofits only -
pMM0652
Plasmid#129013PurposeControl for optogenetic machinery (pMEDIUM-VP16-CIB1/pMEDIUM-ZCRY2PHR) integration effectsDepositorInsert5' URA3-pRPL18B-SV40NLS-VP16-CIB1-tENO1-pRPL18B-SV40NLS-ZCRY2PHR-tSSA1-Spacer-URA3 URA3 3'-KanR-ColE1
UseTagsExpressionYeastMutationPromoterAvailable sinceOct. 16, 2019AvailabilityAcademic Institutions and Nonprofits only -
pTH825-CEN-minRLuc/slowstaCFLuc
Plasmid#115370PurposeExpresses translation elongation-controlled Renilla luciferase and translation initiation-controlled firefly luciferaseDepositorInsertsGcn4 5'-UTR + Firefly Luciferase
Renilla luciferase (codon minimised)
UseTagsExpressionYeastMutationAll codons have been changed for the least favour…PromoterADH1 and TDH3Available sinceMarch 21, 2019AvailabilityAcademic Institutions and Nonprofits only -
pENTR-R4R3-mRuby-N
Plasmid#113752PurposeGateway multi-site entry clone for second position mRuby (N-terminal tag). Introduced into the genome via homology-directed repair after an LR reaction with homology sequences.DepositorInsertmRuby2
UseCRISPR; Gateway entry vectorTagsmRuby2ExpressionMutationPromoterAvailable sinceNov. 1, 2018AvailabilityAcademic Institutions and Nonprofits only -
pENTR-R4R3-2xmEGFP-N
Plasmid#113747PurposeGateway multi-site entry clone for second position 2x mEGFP (N-terminal tag). Introduced into the genome via homology-directed repair after an LR reaction with homology sequences.DepositorInsertmEGFP-mEGFP
UseCRISPR; Gateway entry vectorTags2xmEGFPExpressionMutationPromoterAvailable sinceSept. 24, 2018AvailabilityAcademic Institutions and Nonprofits only -
pENTR-R4R3-3xmEGFP-N
Plasmid#113748PurposeGateway multi-site entry clone for second position 3x mEGFP (N-terminal tag). Introduced into the genome via homology-directed repair after an LR reaction with homology sequences.DepositorInsertmEGFP-mEGFP-mEGFP
UseCRISPR; Gateway entry vectorTags3xmEGFPExpressionMutationPromoterAvailable sinceSept. 24, 2018AvailabilityAcademic Institutions and Nonprofits only -
pENTR-R4R3-2xmEGFP-C
Plasmid#113750PurposeGateway multi-site entry clone for second position 2x mEGFP (C-terminal tag). Introduced into the genome via homology-directed repair after an LR reaction with homology sequences.DepositorInsertmEGFP-mEGFP
UseCRISPR; Gateway entry vectorTags2xmEGFPExpressionMutationPromoterAvailable sinceSept. 24, 2018AvailabilityAcademic Institutions and Nonprofits only -
pENTR-R4R3-2xmRuby-N
Plasmid#113753PurposeGateway multi-site entry clone for second position 2x mRuby (N-terminal tag). Introduced into the genome via homology-directed repair after an LR reaction with homology sequences.DepositorInsertmRuby2-mRuby2
UseCRISPR; Gateway entry vectorTags2xmRuby2ExpressionMutationPromoterAvailable sinceSept. 24, 2018AvailabilityAcademic Institutions and Nonprofits only -
pENTR-R4R3-2xmRuby-C
Plasmid#113756PurposeGateway multi-site entry clone for second position 2x mRuby (C-terminal tag). Introduced into the genome via homology-directed repair after an LR reaction with homology sequences.DepositorInsertmRuby2-mRuby2
UseCRISPR; Gateway entry vectorTags2xmRuby2ExpressionMutationPromoterAvailable sinceSept. 24, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET12a-AL-103 Y96F
Plasmid#47094DepositorInsertAL-103 Y96F (IGKV3-20 Human)
UseTagsompTExpressionBacterialMutationY96FPromoterT7Available sinceAug. 2, 2013AvailabilityAcademic Institutions and Nonprofits only -
pET12a-AL-103 Y32F
Plasmid#47091DepositorInsertAL-103 Y32F (IGKV3-20 Human)
UseTagsompTExpressionBacterialMutationY32FPromoterT7Available sinceAug. 2, 2013AvailabilityAcademic Institutions and Nonprofits only -
pET12a-AL-09 FL Q42K C214S
Plasmid#47089DepositorInsertAL-09 FL Q42K C214S (IGKV3-20 Human)
UseTagsompTExpressionBacterialMutationQ42K C214SPromoterT7Available sinceAug. 2, 2013AvailabilityAcademic Institutions and Nonprofits only -
pET12a-AL-09 FL I34N C214S
Plasmid#47088DepositorInsertAL-09 FL I34N C214S (IGKV3-20 Human)
UseTagsompTExpressionBacterialMutationI34N C214SPromoterT7Available sinceAug. 2, 2013AvailabilityAcademic Institutions and Nonprofits only -
pET12a-AL-09 Y96F
Plasmid#47085DepositorInsertAL-09 Y96F (IGKV3-20 Human)
UseTagsompTExpressionBacterialMutationY96FPromoterT7Available sinceAug. 2, 2013AvailabilityAcademic Institutions and Nonprofits only -
pET12a-AL-09 Y91F
Plasmid#47084DepositorInsertAL-09 Y91F (IGKV3-20 Human)
UseTagsompTExpressionBacterialMutationY91FPromoterT7Available sinceAug. 2, 2013AvailabilityAcademic Institutions and Nonprofits only -
pET12a-AL-09 Y32F Y96F
Plasmid#47083DepositorInsertAL-09 Y32F Y96F (IGKV3-20 Human)
UseTagsompTExpressionBacterialMutationY32F Y96FPromoterT7Available sinceAug. 1, 2013AvailabilityAcademic Institutions and Nonprofits only -
pET12a-AL-09 Y32F
Plasmid#47082DepositorInsertAL-09 Y32F (IGKV3-20 Human)
UseTagsompTExpressionBacterialMutationY32FPromoterT7Available sinceAug. 1, 2013AvailabilityAcademic Institutions and Nonprofits only -
pTSin EGFP
Plasmid#127695PurposePlasmid contains the entire replication competent Sindbis genome with the structural genome components replaced by EGFP in an MCS locus. See Resource Information section for annotated plasmid sequenceDepositorInsertEGFP
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceJuly 3, 2019AvailabilityIndustry, Academic Institutions, and Nonprofits -
pHIV-iRFP720-E2A-Luc
Plasmid#104587PurposeLentivirus vector for bi-cistronic expression of iRFP720 and firefly luciferase from EF1a promoterDepositorInsertiRFP720-E2A-Luciferase
UseLentiviral and LuciferaseTagsbi-cistronicExpressionMammalianMutationPromoterAvailable sinceJan. 9, 2018AvailabilityAcademic Institutions and Nonprofits only -
pPET-PKR/PPase
Plasmid#42934DepositorInsertsUseTagsExpressionBacterialMutationPromoterT7Available sinceApril 12, 2013AvailabilityAcademic Institutions and Nonprofits only -
pBADmod1-linker2-gamS
Plasmid#45833DepositorInsertgamS
UseSynthetic BiologyTagsHistagExpressionBacterialMutationPromoterpBADAvailable sinceMay 4, 2015AvailabilityAcademic Institutions and Nonprofits only -
OmpA-mNG-sfTq2ox
Plasmid#124220PurposePeriplasmic OM bound mNeongreen superfolder mTurquoise2 ox tandem, ~40 % energy transfer. pNM092 - pTHV037-Ompa-SA1-177-(SA-1)-LEDPPAEL-mNG-sfTq2ox,DepositorInsertOmpA-mNG-sfTq2ox
UseTagsExpressionBacterialMutationPromoterAvailable sinceApril 23, 2019AvailabilityAcademic Institutions and Nonprofits only -
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pAAV-S in pAR9 (rep/cap)
Plasmid#174539Purposeencodes AAV-S capsid for efficient gene delivery to the mammalian cochleaDepositorInsertVP1 of AAV9 containing the AAV-S 7-mer peptide insert
UseAAVTagsExpressionMammalianMutationPromoterAvailable sinceOct. 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCAGGS_L_EBOV
Plasmid#103052PurposeEncodes Ebola virus L gene (Zaire ebolavirus, Mayinga) under control of the CAG promoterDepositorInsertL gene of Ebola virus (L Zaire ebolavirus, Mayinga)
UseTagsExpressionMammalianMutationPromoterCAGAvailable sinceDec. 20, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCAGGS_VP35_EBOV
Plasmid#103050PurposeEncodes Ebola virus VP35 gene (Zaire ebolavirus, Mayinga) under control of the CAG promoterDepositorInsertVP35 gene of Ebola virus (VP35 (Zaire ebolavirus, Mayinga))
UseTagsExpressionMammalianMutationPromoterCAGAvailable sinceDec. 20, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCW57.1-Luciferase
Plasmid#99283PurposeAll-in-one lentivector with tet-inducible luciferaseDepositorInsertFirefly luciferase
UseLentiviralTagsExpressionMammalianMutationPromoterTREAvailable sinceAug. 25, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCAGGS_3E5E_luciferase
Plasmid#103055PurposeEncodes Ebola virus minigenome with firefly luciferase reporter gene. Minigenome is under control of the CAG promoterDepositorInsertEbolavirus minigenome
UseLuciferaseTagsExpressionMammalianMutationPromoterCAGAvailable sinceDec. 21, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCAGGS_VP30_EBOV
Plasmid#103051PurposeEncodes Ebola virus VP30 gene (Zaire ebolavirus, Mayinga) under control of the CAG promoterDepositorInsertVP30 gene of Ebola virus (VP30 Zaire ebolavirus, Mayinga)
UseTagsExpressionMammalianMutationPromoterCAGAvailable sinceDec. 20, 2017AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-Plagl2
Plasmid#228061Purposetransiently express mouse Plagl2DepositorInsertPlagl2 (Plagl2 Mouse)
UseTagsExpressionMammalianMutationPromoterAvailable sinceNov. 4, 2024AvailabilityAcademic Institutions and Nonprofits only -
pDOE11 Lat52:mCherry transient
Plasmid#124249PurposeAllows bimolecular fluorescence complementation via transient expression of two proteins in directly transfected pollen tubes.DepositorTypeEmpty backboneUseTagsExpressionPlantMutationPromoterAvailable sinceApril 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
pMM0646
Plasmid#129007PurposeIntegration vector for optogenetic machinery (pSTRONG-VP16-CIB1/pMEDIUM-ZCRY2PHR) and pZF-mRUBY2 with loxable KIURA3 markerDepositorInsertKanR-ColE1 URA 5'-pTEF1-SV40NLS-VP16-CIB1-tENO1-pRPL18B-SV40NLS-ZCRY2PHR-tSSA1-pZF(BS)-mRUBY2-tADH1-loxP-KIURA3-loxP-URA3 3'
UseTagsExpressionYeastMutationPromoterAvailable sinceOct. 16, 2019AvailabilityAcademic Institutions and Nonprofits only -
pHDR-USR-eGFP
Plasmid#208830PurposeHDR Universal Surrogate Reporter with a sgRNA and consistently expressed eGFP but without Cas9. The selective PuroR reporter gene was designed to be repaired only by the sgRNA/Cas9-triggered HDR.DepositorTypeEmpty backboneUseTagsExpressionMammalianMutationPromoterCMV, U6Available sinceNov. 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
E407V Nuclear Myosin I YFP
Plasmid#60242PurposeExpresses a E407V mutant of the nuclear isoform of myosin IC attached to YFP and an NLS, which has impaired actin binding and motor activitiesDepositorInsertNuclear Myosin 1 (Myo1c Mouse)
UseTagsEYFP and NLSExpressionMammalianMutationE407VPromoterAvailable sinceNov. 21, 2014AvailabilityAcademic Institutions and Nonprofits only -
pL-SFFV.Reporter.RFP657.PAC
Plasmid#61395PurposeLentiviral vector for RFP657 NHEJ reporter system expression. Allows insertion of target sites between the SFFV promoter and RFP657 for detection of fluorescence knockdown. Puromycin resistance.DepositorTypeEmpty backboneUseLentiviralTagsExpressionMammalianMutationPromoterSFFVAvailable sinceMay 18, 2015AvailabilityAcademic Institutions and Nonprofits only -
pET28a-B4E
Plasmid#162067PurposeFor expresion of the B4E fusion protein, consists of the ampR gene (encoding the enzyme β-lactamase without the native signal sequence) fused to residues 28-215 of the murine eIF4EDepositorInsertampR-eIF4E fusion protein
UseTags6xHis tagExpressionBacterialMutationeIF4E incorporating the K119A mutation, which is …PromoterT7Available sinceFeb. 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLenti-U6sgbbBsmbI-puro-2A-Fluc
Plasmid#100277PurposeLentiviral vector for CRISPR mediated gene editing in cells while expressing Firefly Luciferase for in vivo imagingDepositorTypeEmpty backboneUseCRISPR and LentiviralTagsExpressionMutationPromoterAvailable sinceOct. 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
OmpA-mNG-mCh
Plasmid#124216PurposePeriplasmic OM bound mNeongreen mCherry tandem, ~16 % energy transfer. pNM004 - pTHV037-OmpA-SA1-177-(SA-1)-LEDPPAEL-mNG-mChDepositorInsertOmpA-mNG-mCh
UseTagsExpressionBacterialMutationPromoterAvailable sinceMay 8, 2019AvailabilityAcademic Institutions and Nonprofits only