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We narrowed to 18,759 results for: nls

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Showing: 4721 - 4760 of 18759 results

  5. pHR_Gal4UAS_4D5-High-CAR-mCherry_PGK_4D5-Low-SynNotch_Gal4VP64_RHL133

    Plasmid
    #164822
    Purpose
    Lentiviral vector for Gal4 inducible AntiHER2 4D5-High CAR-mCherry and constitutive expression of antiHER2 4D5-Low Gal4VP64 Synthetic Notch
    Depositor
    Wendell Lim
    Publication
    Hernandez-Lopez et al Science. 2021 Mar 12;371(6534):1166-1171
    Inserts
    AntiHER2 4D5-High Chimeric Antigen receptor (CD8alphaTM-41BB-CD3z)
    AntiHER2 4D5-Low Gal4VP64 synthetic Notch receptor
    Use
    Lentiviral
    Tags
    mCherry
    Promoter
    miniCMV and pGK
    Available since
    June 28, 2022
    Availability
    Academic Institutions and Nonprofits only
    164822-plasmid-map-sequence-id-330530

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  11. pMGS56 (GFP-ARF16-PB1-P2A-OsTIR1)

    Plasmid
    #129668
    Purpose
    A repair construct to express GFP-ARF16-PB1 and OsTIR1 under the control of the CMV promoter from the human AAVS1 locus
    Depositor
    Michael Guertin
    Publication
    Sathyan et al Genes Dev. 2019 Oct 1;33(19-20):1441-145
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    OsARF16-PB1 domain
    Tags
    GFP
    Expression
    Mammalian
    Available since
    Aug. 15, 2019
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    129668-plasmid-map-sequence-id-254335

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  14. pCMV-dCpf1-eBE

    Plasmid
    #107688
    Purpose
    Expresses dLbCpf1-eBE in mammalian cells
    Depositor
    Jia Chen
    Publication
    Li et al Nat Biotechnol. 2018 Mar 19. pii: nbt.41
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    dLbCpf1-eBE
    Expression
    Mammalian
    Mutation
    dLbCpf1(D832A/E925A/D1148A)
    Promoter
    CMV
    Available since
    April 25, 2018
    Availability
    Academic Institutions and Nonprofits only
    107688-plasmid-map-sequence-id-207603

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  15. PB-SAM-Dest

    Plasmid
    #102563
    Purpose
    Piggybac transposon DEST vector encoding dCAS9-VP64 and MS2-P65-HSF1 activator helper complex. It is used for insertion of multiple U6-sgRNA expression cassettes.
    Depositor
    Ying Liu
    Publication
    Li et al Mol Ther Nucleic Acids. 2017 Sep 15;8:64
    Type
    Empty backbone
    Use
    CRISPR; Piggybac transposon
    Expression
    Mammalian
    Promoter
    N/A
    Available since
    Jan. 5, 2018
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    102563-plasmid-map-sequence-id-504859

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  17. pLenti_dCas9-2xAM

    Plasmid
    #92220
    Purpose
    Lentiviral plasmid expressing dCas9-2xAM and gRNA cloned in Bbs I sites
    Depositor
    Hodaka Fujii
    Publication
    Fujita et al BMC Res Notes. 2018 Feb 27;11(1):154. do
    Inserts
    Sp-dCas9-2xAM tag
    gRNA to be inserted into Bbs I sites
    Use
    CRISPR and Lentiviral
    Tags
    2xAM tag
    Expression
    Mammalian
    Mutation
    human codon-optimized, D10A + H840A
    Promoter
    CBh and U6
    Available since
    June 12, 2017
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    92220-plasmid-map-sequence-id-181084

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  27. pSH-Csy4-T2A-SpRFN

    Plasmid
    #85754
    Purpose
    Expresses S. pyogenes FokI-dCas9-NLS with a 25 amino acid linker (GGGGS)5 fusion. Also expresses Csy4 for cleavage of multiplexed gRNA transcripts. Derived from pSQT1601 (Addgene #53369).
    Depositor
    Lawrence Stanton
    Publication
    Havlicek et al Mol Ther. 2017 Feb 1;25(2):342-355. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Csy4-T2A-FokI-dCas9
    Use
    CRISPR
    Tags
    Csy4 and FokI fusion via 25 amino acid (GGGGS)5 l…
    Expression
    Mammalian
    Mutation
    D10A, H840A
    Promoter
    CAG
    Available since
    Jan. 20, 2017
    Availability
    Academic Institutions and Nonprofits only
    85754-plasmid-map-sequence-id-170272

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  29. pCM66

    Plasmid
    #261136
    Purpose
    PAR-6::VC155 and mCherry expression under elt-2 minimal promoter expression. Can be integrated at single-copy into genome using RMCE.
    Depositor
    John Calarco
    Publication
    Martin et al bioRxiv 2025.02.28.640866
    Insert
    elt-2p::AID::nls::mcherry::2a::AID::PAR-6::VC155::tbb-2 3' in pLF3FShC (par-6 Nematode)
    Expression
    Worm
    Mutation
    PAR-6
    Promoter
    elt-2 minimal promoter
    Available since
    Aug. 31, 2026
    Availability
    Academic Institutions and Nonprofits only
    261136-plasmid-map-sequence-id-520099

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  31. pCC_11 - hU6-BsmBI-sgRNA(E+F)-barcode-EFS-KRAB-dxCas9-NLS-2A-Puro-WPRE

    Plasmid
    #139096
    Purpose
    Expresses human codon-optimized inactive xCas9 3.7 fused to a transcriptional repressor KRAB in mammalian cells. For cloning of sgRNAs using BsmBI. Contains a barcode downstream of sgRNA cassette.
    Depositor
    Neville Sanjana
    Publication
    Legut et al Cell Rep. 2020 Mar 3;30(9):2859-2868.e5.
    Insert
    KRAB-dxCas9 3.7
    Use
    CRISPR and Lentiviral
    Expression
    Mammalian
    Mutation
    D10A, H840A, A262T, R324L,S409I, E480K, E543D, M6…
    Available since
    March 6, 2020
    Availability
    Academic Institutions and Nonprofits only
    139096-plasmid-map-sequence-id-273894

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  34. Click editor (CE1) - pCMV-T7-PCV2-nCas9-EcKlenow (EMK488)

    Plasmid
    #208942
    Purpose
    The CE1 construct, expressed from CMV or T7 promoters.
    Depositor
    Benjamin Kleinstiver
    Publication
    Ferreira da Silva et al Nat Biotechnol. 2024 Jul 22. doi: 10.103
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    PCV2-XTEN-nSpCas9-BPNLS-EcKlenow(-exo)-BPNLS
    Use
    CRISPR; In vitro transcription; t7 promoter
    Tags
    BPNLS
    Expression
    Mammalian
    Mutation
    nSpCas9(H840A); EcKlenow(-exo;D355A/E357A)
    Promoter
    CMV and T7
    Available since
    Nov. 22, 2023
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    208942-plasmid-map-sequence-id-415667

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  38. pM-Cas12a

    Plasmid
    #196291
    Purpose
    35S promoter-driven expression of the positive-sense TSWV M RNA segment encoding LbCas12a in place of viral GP
    Depositor
    Zhenghe Li
    Publication
    Liu et al Mol Plant. 2023 Mar 6;16(3):616-631. doi
    Insert
    Full length TSWV M antigenome encoding LbCas12a in place of viral GP (cas12a Synthetic)
    Use
    CRISPR
    Tags
    Flag
    Expression
    Plant
    Mutation
    See depositor comments below
    Promoter
    duplicated cauliflower mosaic virus 35S promoter
    Available since
    May 3, 2023
    Availability
    Academic Institutions and Nonprofits only
    196291-plasmid-map-sequence-id-389824

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  39. pEF_PE2

    Plasmid
    #199267
    Purpose
    Mammalian expression of SpCas9 prime editor 2 under EF1α promoter
    Depositor
    Ting Zhou
    Publication
    Li et al Nat Commun. 2022 Oct 27;13(1):6354. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    PE2-P2A-mp53DD
    Expression
    Mammalian
    Mutation
    Δ40-903
    Available since
    April 13, 2023
    Availability
    Academic Institutions and Nonprofits only
    199267-plasmid-map-sequence-id-390160

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Showing: 4721 - 4760 of 18759 results