We narrowed to 5,036 results for: U6...

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AY22_pgRNA.R5.1

Plasmid

#100294

Purpose

CCR5-targeting gRNA expression plasmid

Depositor

Manuel Goncalves

Article

Chen et al Nat Commun. 2017 Sep 22;8(1):657. doi: 1

Insert

gRNA spacer

Use

CRISPR and Synthetic Biology

Expression

Mammalian

Promoter

U6 RNA Pol III promoter human snRNA

Available Since

Sept. 22, 2017

Availability

Academic Institutions and Nonprofits only

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AV44_pCAG.Cas9-D10A.gRNA.S1

Plasmid

#199258

Purpose

Expression construct encoding SpCas9-D10A nickase and an AAVS1-targeting guide RNA

Depositor

Manuel Goncalves

Article

Wang et al Nucleic Acids Res. 2021 Jan 25;49(2):117

Inserts

SpCas9-D10A nickase
AAVS1-targeting gRNA

Use

CRISPR

Tags

SV40 NLS

Expression

Mammalian

Mutation

D10A

Promoter

CAG promoter and RNA polymerase III promoter for …

Available Since

April 28, 2023

Availability

Academic Institutions and Nonprofits only

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AV13_pCAG.Cas9.gRNA.NT

Plasmid

#199260

Purpose

Expression construct encoding SpCas9 nuclease and a control non-targeting guide RNA

Depositor

Manuel Goncalves

Article

Wang et al Nucleic Acids Res. 2021 Jan 25;49(2):117

Inserts

SpCas9 nuclease
Non-targeting (NT) control gRNA

Use

CRISPR

Tags

SV40 NLS

Expression

Mammalian

Promoter

CAG promoter and RNA polymerase III promoter for …

Available Since

April 27, 2023

Availability

Academic Institutions and Nonprofits only

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DKC1 F1.3 gRNA

Plasmid

#90652

Purpose

3rd generation lentiviral gRNA plasmid targeting human DKC1

Depositor

Iain Cheeseman

Article

McKinley et al Dev Cell. 2017 Feb 27;40(4):405-420.e2.

Insert

DKC1 (Guide Designation F1.3)

Use

CRISPR and Lentiviral

Promoter

U6

Available Since

July 20, 2017

Availability

Academic Institutions and Nonprofits only

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DKC1 F2.3 gRNA

Plasmid

#90653

Purpose

3rd generation lentiviral gRNA plasmid targeting human DKC1

Depositor

Iain Cheeseman

Article

McKinley et al Dev Cell. 2017 Feb 27;40(4):405-420.e2.

Insert

DKC1 (Guide Designation F2.3)

Use

CRISPR and Lentiviral

Promoter

U6

Available Since

May 31, 2019

Availability

Academic Institutions and Nonprofits only

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pC0043-PspCas13b crRNA backbone

Plasmid

#103854

Purpose

For cloning of guide RNAs compatible with PspCas13b. Contains a 3' direct repeat. Clone using BbsI (BpiI). F overhang cacc. R overhang caac

Depositor

Feng Zhang

Article

Cox et al Science. 2017 Oct 25. pii: eaaq0180. doi

Has Service

Cloning Grade DNA

Insert

U6-BbsI-BbsI-PspCas13b DR-polyT

Use

CRISPR

Expression

Mammalian

Available Since

Nov. 28, 2017

Availability

Industry, Academic Institutions, and Nonprofits

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PX333-T2A-EGFP

Plasmid

#197417

Purpose

SpCas9-T2A-EGFP with dual sgRNA cloning backbones

Depositor

Yeh-Hsing Lao

Article

Yoshinaga et al Nano Lett. 2023 Jan 17. doi: 10.1021/acs

Type

Empty backbone

Use

CRISPR

Expression

Mammalian

Promoter

CBh; U6

Available Since

March 30, 2023

Availability

Academic Institutions and Nonprofits only

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pHSG1C3

Plasmid

#164423

Purpose

single guide RNA (sgRNA) and Prime editing guide RNA (pegRNA) cloning and mammalian cell expression. BbsI cloning for sgRNAs and BbsI/PstI for pegRNAs.

Depositor

Xiao Wang

Article

Standage-Beier et al ACS Synth Biol. 2021 Feb 19;10(2):422-42

Insert

sgRNA

Use

CRISPR and Synthetic Biology

Expression

Mammalian

Promoter

U6

Available Since

Aug. 4, 2021

Availability

Academic Institutions and Nonprofits only

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pSLQ-mgU2-47-Mango II

Plasmid

#127586

Purpose

Expresses mgU2-47-Mango II scaRNA from a mU6 promoter

Depositor

David Rueda

Article

Autour et al Nat Commun. 2018 Feb 13;9(1):656. doi: 1

Insert

mgU2-47 scaRNA with Mango II tag

Expression

Mammalian

Promoter

modified U6

Available Since

Oct. 4, 2019

Availability

Academic Institutions and Nonprofits only

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pLib6.4B-Aalb_743

Plasmid

#176676

Purpose

Expression of sgRNA under Ae. albopictus U6 (AALF029743) promoter & delivery of D.mel-Actin5C::EBFP2-2A-puro|sgRNA cassette through PhiC31 RCME to AttP acceptor cell line/organism

Depositor

Norbert Perrimon

Article

Viswanatha et al Nat Commun. 2021 Nov 24;12(1):6825. doi:

Type

Empty backbone

Use

Crispr

Expression

Insect

Available Since

Nov. 23, 2021

Availability

Academic Institutions and Nonprofits only

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pSLQ-5S-F30-Mango IV

Plasmid

#127584

Purpose

Expresses 5S-F30-Mango IV rRNA from a mU6 promoter

Depositor

David Rueda

Article

Autour et al Nat Commun. 2018 Feb 13;9(1):656. doi: 1

Insert

5S rRNA with F30 folding scaffold and Mango IV tag

Expression

Mammalian

Promoter

modified U6

Available Since

Oct. 4, 2019

Availability

Academic Institutions and Nonprofits only

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px330-mcherry

Plasmid

#98750

Purpose

Cas9 from S.pyogenes with CMV-mcherry cassette, and cloning backbone for sgRNA

Depositor

Jinsong Li

Article

Wu et al Cell Stem Cell. 2013 Dec 5;13(6):659-62.

Type

Empty backbone

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available Since

Oct. 30, 2017

Availability

Academic Institutions and Nonprofits only

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LRG2.1

Plasmid

#108098

Purpose

Lentiviral expression plasmid of sgRNA with GFP

Depositor

Christopher Vakoc

Article

Tarumoto et al Mol Cell. 2018 Feb 28. pii: S1097-2765(1

Type

Empty backbone

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

U6 promoter for sgRNA expression and EFS promoter…

Available Since

June 1, 2018

Availability

Academic Institutions and Nonprofits only

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pBSU6-shTim23/CMV-eGFP

Plasmid

#89795

Purpose

Expresses shRNA against TIM23 with a separate promoter for expression of GFP.

Depositor

Robert Friedlander

Article

Yano et al Nat Neurosci. 2014 Jun;17(6):822-31. doi

Insert

shRNA Tim23 (Timm23 Mouse)

Tags

eGFP under separate promoter

Expression

Mammalian

Promoter

U6

Available Since

May 31, 2017

Availability

Academic Institutions and Nonprofits only

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LRCherry2.1

Plasmid

#108099

Purpose

Lentiviral expression plasmid of sgRNA with mCherry

Depositor

Christopher Vakoc

Article

Tarumoto et al Mol Cell. 2018 Feb 28. pii: S1097-2765(1

Type

Empty backbone

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

U6 promoter for sgRNA expression and EFS promoter…

Available Since

May 8, 2018

Availability

Academic Institutions and Nonprofits only

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pLib6.4B-Aaeg_774

Plasmid

#176673

Purpose

Expression of sgRNA under Ae. Aegypti U6 (AAEL017774) promoter & delivery of D.mel-Actin5C::EBFP2-2A-puro|sgRNA cassette through PhiC31 RCME to AttP acceptor cell line/organism

Depositor

Norbert Perrimon

Article

Viswanatha et al Nat Commun. 2021 Nov 24;12(1):6825. doi:

Type

Empty backbone

Use

Crispr

Expression

Insect

Available Since

Nov. 24, 2021

Availability

Academic Institutions and Nonprofits only

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LRG2.1_Puro

Plasmid

#125594

Purpose

Lentiviral expression of sgRNA with GFP and puromycin resistance gene

Depositor

Christopher Vakoc

Article

Tarumoto et al Blood. 2019 Nov 1. pii: 422697. doi: 10.

Type

Empty backbone

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

U6 promoter for sgRNA expression and EFS promoter…

Available Since

June 10, 2019

Availability

Academic Institutions and Nonprofits only

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eSpCas9(1.1)_No_FLAG_AAVS1_T2

Plasmid

#79888

Purpose

Expresses the AAVS1 T2 gRNA in combination with FLAGless eSpCas9(1.1) to target the AAVS1 "safe harbor" locus.

Depositor

Yannick Doyon

Article

Dalvai et al Cell Rep. 2015 Oct 7. pii: S2211-1247(15

Type

Empty backbone

Use

CRISPR

Tags

Untagged eSpCas9(1.1)

Expression

Mammalian

Promoter

CBh

Available Since

July 29, 2016

Availability

Academic Institutions and Nonprofits only

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Cas9 sgRNA vector

Plasmid

#68463

Purpose

Cas9 sgRNA vector for cloning

Depositor

Su-Chun Zhang

Article

Chen et al Cell Stem Cell. 2015 Jul 1. pii: S1934-5

Type

Empty backbone

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available Since

Nov. 25, 2015

Availability

Academic Institutions and Nonprofits only

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PX552

Plasmid

#60958

Purpose

pAAV-U6sgRNA(SapI)_hSyn-GFP-KASH-bGH (SpGuide acceptor). AAV plasmid for sgRNA cloning. GFP-KASH fusion facilitates FACS sorting of cells and nuclei.

Depositor

Feng Zhang

Article

Swiech et al Nat Biotechnol. 2014 Oct 19. doi: 10.103

Inserts

U6_(SpaI)_sgRNA
Syn_EGFP-KASH

Use

AAV, CRISPR, and Mouse Targeting

Expression

Mammalian

Promoter

Synapsin and U6

Available Since

Nov. 17, 2014

Availability

Academic Institutions and Nonprofits only

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