4,075 results

1. pCAG-CreERT2

   Plasmid

   #14797

   Purpose

   Mammalian expression of conditionally active form of Cre recombinase (not as tightly regulated as pCAG-ERT2CreERT2)

   Depositor

   Connie Cepko

   Article

   Matsuda et al Proc Natl Acad Sci U S A. 2007 Jan 16;10

   Insert

   Cre-ERT2 fusion protein
   

   Use

   Cre/Lox

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   April 20, 2007

   Availability

   Academic Institutions and Nonprofits only

   

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2. pLentiPGK Blast DEST ERKKTRmRuby2

   Plasmid

   #90231

   Purpose

   Lentiviral vector to express ERK KTR mRuby2 under PGK promoter (With Blasticidin Resistance)

   Depositor

   Markus Covert

   Article

   Kudo et al Nat Protoc. 2018 Jan;13(1):155-169. doi:

   Insert

   ERK Kinase Translocation Reporter
   

   Use

   Lentiviral

   Tags

   mRuby2

   Expression

   Mammalian

   Mutation

   Promoter

   PGK

   Available since

   March 17, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. mEmerald-N1

   Plasmid

   #53976

   Purpose

   Localization: N1 Cloning Vector, Excitation: 487, Emission: 509

   Depositor

   Michael Davidson

   Type

   Empty backbone

   Use

   Tags

   mEmerald

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   June 16, 2014

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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4. pRSFDuet-1(nsp8-nsp7)(nsp12)

   Plasmid

   #165451

   Purpose

   Expresses the RNA Dependent RNA polymerase complex of SARS-CoV-2 in Escherichia Coli

   Depositor

   Marc Delarue

   Article

   Madru et al PLoS One. 2021 Apr 29;16(4):e0250610. do

   Inserts

   nsp12
   nsp8
   nsp7
   

   Use

   Tags

   14Histidine-TEV

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   May 25, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pANAP

   Plasmid

   #48696

   Purpose

   Allows co-translational incorporation of the fluorescent ncAA Anap directly into the target protein; expresses amber suppressor tRNA and the synthetase AnapRS

   Depositor

   Peter Schultz

   Article

   Chatterjee et al J Am Chem Soc. 2013 Aug 28;135(34):12540

   Inserts

   8 copies of the amber suppressor tRNACUA-Leu
   AnapRS
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   mutant E.coli leucyl synthetase specific for Anap…

   Promoter

   CMV and human H1 promoter

   Available since

   Nov. 25, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCI HA NEDD4L

   Plasmid

   #27000

   Depositor

   Joan Massague

   Article

   Gao et al Mol Cell. 2009 Nov 13. 36(3):457-68.

   Insert

   NEDD4L (NEDD4L Human)
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Jan. 5, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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7. rAAV2/MG1.1

   Plasmid

   #184540

   Purpose

   AAV packaging plasmid, expressing Rep2 and AAV-MG1.1 capsid

   Depositor

   Minmin Luo

   Article

   Lin et al Nat Methods. 2022 Jul 25. pii: 10.1038/s

   Insert

   AAV-MG1.1 capsid
   

   Use

   AAV; Helper vector for packaging

   Tags

   Expression

   Mutation

   changes on AAV9 capsid: K449R, A587L, Q588M; inse…

   Promoter

   Available since

   July 22, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. pAcBac1.tR4-MbPyl

   Plasmid

   #50832

   Purpose

   baculovirus-based delivery system that enables the efficient incorporation of unnatural amino acids into proteins in mammalian cells

   Depositor

   Peter Schultz

   Article

   Chatterjee et al Proc Natl Acad Sci U S A. 2013 Jul 16;11

   Inserts

   two-copy Pyl tRNA cassette
   MbPyl
   two copy Pyl tRNA cassette
   

   Use

   Baculoviral

   Tags

   Expression

   Insect

   Mutation

   Promoter

   CMV and U6 and H1

   Available since

   Feb. 13, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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9. pTD103luxI_sfGFP

   Plasmid

   #48885

   Purpose

   Positive feedback plasmid from oscillator

   Depositor

   Jeff Hasty

   Article

   Prindle et al Nature. 2011 Dec 18;481(7379):39-44. doi

   Insert

   LuxI, sfGFP, and LuxR
   

   Use

   Synthetic Biology

   Tags

   ssrA tag (AANDENYALAA)

   Expression

   Bacterial

   Mutation

   Promoter

   pLux

   Available since

   Feb. 6, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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10. pT3-EF1a-c-Met

    Plasmid

    #31784

    Depositor

    Xin Chen

    Article

    Tward et al Proc Natl Acad Sci U S A. 2007 Sep 11;10

    Insert

    Met (MET Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1alpha

    Available since

    Oct. 21, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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