2,226 results

1. AAV Cag Flex H2B Gcamp6f reverse

   Plasmid

   #74155

   Purpose

   Calcium Sensor

   Depositor

   Loren Looger

   Insert

   Gcamp6f
   

   Use

   AAV

   Tags

   H2B

   Expression

   Mutation

   Promoter

   CAG

   Available since

   April 19, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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2. pVQ CMV NanoV1-2a-EGFP ferritin

   Plasmid

   #79649

   Purpose

   Expresses camelid anti-GFP nanobody fused to TRPV1 and GFP-ferritin chimera fusion protein

   Depositor

   Jeffrey Friedman

   Article

   Stanley et al Nature. 2016 Mar 31;531(7596):647-50. do

   Inserts

   transient receptor potential vanillin 1 (Trpv1 Rat)
   camelid anti-GFP nanobody
   Enhanced green fluorescent protein
   mouse ferritin light chain (Ftl1 Mouse)
   mouse ferritin heavy chain (Fth1 Mouse)
   

   Use

   Adenoviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Aug. 16, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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3. His-GFP SNAP25

   Plasmid

   #170872

   Purpose

   Mammalian expression of His-GFP SNAP25.

   Depositor

   Justin Taraska

   Article

   Prasai et al Nat Commun. 2021 Jun 25;12(1):3970. doi:

   Insert

   SNAP 25 (Snap25 Rat)
   

   Use

   Tags

   (His)6 and EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   June 22, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. FUGW-VGLUT2-pHluorin

   Plasmid

   #207469

   Purpose

   Lentiviral expression of VGLUT2-pHluorin

   Depositor

   Robert Edwards

   Article

   Silm et al Neuron. 2019 May 22;102(4):786-800.e5. d

   Insert

   VGLUT2 (Slc17a6 Rat)
   

   Use

   Lentiviral

   Tags

   pHluorin

   Expression

   Mutation

   Promoter

   Available since

   Sept. 13, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pGP-AAV-syn-jGCaMP7s-WPRE

   Plasmid

   #104487

   Purpose

   AAV-mediated expression of ultrasensitive protein calcium sensor under the Syn promoter

   Depositor

   Douglas Kim, GENIE Project

   Article

   Dana et al Nat Methods. 2019 Jul;16(7):649-657. doi

   Has Service

   AAV PHP.eB, AAV Retrograde, AAV1, and AAV9

   Insert

   jGCaMP7s
   

   Use

   AAV

   Tags

   T7 epitope, Xpress tag, 6xHis

   Expression

   Mammalian

   Mutation

   Promoter

   Synapsin

   Available since

   April 20, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. Rlucrarr3-Flash5

   Plasmid

   #74133

   Purpose

   FlAsH-BRET reporter to monitor conformational changes in Arrestin3. Insertion of FlAsH motif (CCPGCC) following amino acid residue 263 of Arrestin.

   Depositor

   Louis Luttrell

   Article

   Lee et al Nature. 2016 Mar 31;531(7596):665-8. doi

   Insert

   Arrestin3
   

   Use

   Tags

   Renilla luciferase (rLuc)

   Expression

   Mammalian

   Mutation

   Insertion of FlAsH motif (CCPGCC) following amino…

   Promoter

   Available since

   April 1, 2016

   Availability

   Academic Institutions and Nonprofits only

7. p3 FLAG_CMV14/B0,+

   Plasmid

   #112798

   Purpose

   Expression of ATB0,+

   Depositor

   Katarzyna Nalęcz

   Article

   Samluk et al Biochem Biophys Res Commun. 2012 May 25;

   Insert

   B(0,+)/SLC6A14 (Slc6a14 Rat)
   

   Use

   Tags

   3 x Flag

   Expression

   Mammalian

   Mutation

   L350S

   Promoter

   Available since

   Sept. 7, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. p3 FLAG_CMV14/OCTN2 (wt)

   Plasmid

   #112799

   Purpose

   Expression of OCTN2

   Depositor

   Katarzyna Nalęcz

   Article

   Czeredys et al PLoS One. 2013 Dec 13;8(12):e82105. doi:

   Insert

   OCTN2/SLC22A5 (Slc22a5 Rat)
   

   Use

   Tags

   3 x Flag

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 23, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. pmcherry-LCa

   Plasmid

   #53972

   Purpose

   Expresses the light chain of clathrin

   Depositor

   Tom Kirchhausen

   Article

   Kural et al Cell Rep. 2012 Nov 29;2(5):1111-9. doi:

   Insert

   clathrin light chain
   

   Use

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Aug. 1, 2014

   Availability

   Academic Institutions and Nonprofits only

10. pFsynW SYP-GFP

    Plasmid

    #197283

    Purpose

    Encodes for viral expression of full-length wild type synaptophysin (SYP). Visualized by a C-terminal GFP.

    Depositor

    Edwin Chapman

    Article

    Watson et al Elife. 2023 Feb 2;12:e82568. doi: 10.755

    Insert

    SYP and GFP (Syp Rat)
    

    Use

    Lentiviral

    Tags

    1D4 epitope

    Expression

    Mutation

    N/A

    Promoter

    hSyn (human synapsin I promoter)

    Available since

    July 28, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. pAAV-EF1a-DIO-PSD95-EGFP-WPRE

    Plasmid

    #133785

    Purpose

    Cre-dependent EGFP Fluorescent reporter for overexpressed postsynaptic marker PSD95 in mammalian cell

    Depositor

    Michael Stryker

    Article

    Sun et al Proc Natl Acad Sci U S A. 2019 Oct 22;11

    Insert

    PSD95-EGFP (Dlg4 Rat)
    

    Use

    AAV and Cre/Lox

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 23, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. pRSET-RCaMP1h

    Plasmid

    #42874

    Purpose

    a single-wavelength red-shifted genetically encoded calcium indicator constructed from calmodulin and cp-mRuby

    Depositor

    Loren Looger

    Article

    Akerboom et al Front Mol Neurosci. 2013;6:2. doi: 10.33

    Insert

    Red Fluorescent Calcium binding Protein
    

    Use

    Tags

    EK (Enterokinase) Recognition Site, His Tag (6x),…

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Feb. 22, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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13. pEGFP-C1-PRKAA1

    Plasmid

    #30305

    Depositor

    Jay Brenman

    Article

    Kazgan et al Mol Biol Cell. 2010 Oct 1. 21(19):3433-4

    Insert

    AMP-activated Protein Kinase α1 (Prkaa1 Rat)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 21, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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14. CMV::SypHy A4

    Plasmid

    #24478

    Depositor

    Leon Lagnado

    Article

    Granseth et al Neuron. 2006 Sep 21. 51(6):773-86.

    Insert

    SypHy (Syp Rat)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 1, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCMV-GCaMP5G

    Plasmid

    #31788

    Purpose

    a single-wavelength GCaMP3-based calcium indicator with improved response. Please also see the GCaMP6s/m/f indicators.

    Depositor

    Loren Looger, Douglas Kim, GENIE Project

    Article

    Akerboom et al J Neurosci. 2012 Oct 3;32(40):13819-40.

    Insert

    GCaMP5G
    

    Use

    Tags

    6xHis, T7 epitope, and Xpress tag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 22, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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16. pLenti UBC LC3Flux (HaloTag7-mGFP-LC3-STOP)

    Plasmid

    #215014

    Purpose

    Express an autophagy (LC3) reporter in mammalian cells (untested)

    Depositor

    Wade Harper

    Insert

    MAP1LC3B (Map1lc3b Rat)
    

    Use

    Lentiviral

    Tags

    HaloTag(7)-mGFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. pORANGE Dlg4-GFP KI

    Plasmid

    #131477

    Purpose

    Endogenous tagging of PSD95: C-terminal (amino acid position: R721)

    Depositor

    Harold MacGillavry

    Article

    Willems et al PLoS Biol. 2020 Apr 10;18(4):e3000665. d

    Insert

    gRNA and GFP donor (Dlg4, Dlg4 Mouse, Rat)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6 and Cbh

    Available since

    Sept. 19, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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18. pCMV-APOBEC1-YTHmut

    Plasmid

    #131637

    Purpose

    Expresses APOBEC1 fused to a mutant YTH domain with diminished m6A binding

    Depositor

    Kate Meyer

    Article

    Meyer et al Nat Methods. 2019 Sep 23. pii: 10.1038/s

    Insert

    APOBEC1-YTHmut-HA (YTHDF2 Human, Rat)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    YTH domain lacks AA 385-409 comprising the m6A-bi…

    Promoter

    CMV

    Available since

    Oct. 23, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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19. Lamp1-YFP

    Plasmid

    #1816

    Purpose

    Mammalian expression of Lamp1 fused to YFP. Used for localization to the lysosome.

    Depositor

    Walther Mothes

    Article

    Sherer et al Traffic 2003 Nov;4(11):785-801.

    Insert

    lysosome associated membrane protein 1 (Lamp1 Rat)
    

    Use

    Tags

    YFP

    Expression

    Mammalian

    Mutation

    D50E (not important for function of plasmid)

    Promoter

    Available since

    Feb. 16, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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20. pAAV-hSyn-DIO-rM3D(Gs)-mCherry

    Plasmid

    #50458

    Purpose

    Double floxed Gs-coupled rM3D DREADD fused with mCherry under the control of human synapsin promoter

    Depositor

    Bryan Roth

    Has Service

    AAV8

    Insert

    rM3D-mCherry
    

    Use

    AAV

    Tags

    HA and mCherry

    Expression

    Mutation

    See supplemental documents for DREADD mutations

    Promoter

    human Synapsin 1

    Available since

    April 16, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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