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We narrowed to 1,334 results for: piggyBac

Showing: 441 - 480 of 1334 results

  1. PB-iRFP-STOP-ReNL

    Plasmid
    #113965
    Purpose
    Piggybac transposon plasmid CRISPR gene deletion activatable fluorescence. Constitutive iRFP670 under EF1A promoter, CMV promoter with two SV40 polyA followed by red-enhanced nanolantern (ReNL)
    Depositor
    Jordan Green
    Article
    Rui et al Sci Adv. 2019 Dec 6;5(12):eaay3255. doi:
    Inserts
    iRFP670
    ReNL
    Use
    Piggybac transposon
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CMV - SV40-PolyA - SV40-PolyA and EF1A
    Available Since
    Dec. 9, 2019
    Availability
    Academic Institutions and Nonprofits only
    113965-plasmid-map-sequence-id-223037

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  5. EW371 (PB) (ZNF35/ZNF35tar-6bp spacers)x4-H2B-BFP insul CMV-TO-ZNF35(5-8)-ZNF35(5-8)-deimlink-NZF

    Plasmid
    #236173
    Purpose
    PiggyBac-integrated plasmid containing hygromycin resistance gene, ZNF35/ZNF35-driven BFP, and ZNF35/ZNF35-NZF transcription factor
    Depositor
    Xiaojing Gao
    Article
    Wolfsberg et al bioRxiv 2025.02.24.639918
    Inserts
    ZNF35(5-8)-ZNF35(5-8)-deImmunLink-NZF
    H2B (H2BC11 Human)
    Use
    Tags
    mTagBFP2
    Expression
    Mammalian
    Mutation
    Promoter
    CMV and four ZNF35/ZNF35 sites
    Available Since
    May 2, 2025
    Availability
    Academic Institutions and Nonprofits only
    236173-plasmid-map-sequence-id-471240

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  6. EW370 (PB) (ZNF35/ZNF250tarv3-6bp spacers)x4-H2B-BFP insul CMV-TO-ZNF35(5-8)-ZNF250(1-4)-deimlink-NZF

    Plasmid
    #236172
    Purpose
    PiggyBac-integrated plasmid containing hygromycin resistance gene, ZNF35/ZNF250-driven BFP, and ZNF35/ZNF250-NZF transcription factor
    Depositor
    Xiaojing Gao
    Article
    Wolfsberg et al bioRxiv 2025.02.24.639918
    Inserts
    ZNF35(5-8)-ZNF250(1-4)-deImmunLink-NZF
    H2B (H2BC11 Human)
    Use
    Tags
    mTagBFP2
    Expression
    Mammalian
    Mutation
    Promoter
    CMV and four ZNF35/ZNF250 sites
    Available Since
    April 9, 2025
    Availability
    Academic Institutions and Nonprofits only
    236172-plasmid-map-sequence-id-468453

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  7. EW372 (PB) (ZNF35/IKZF1tar-6bp spacers)x4-H2B-BFP insul CMV-TO-ZNF35(5-8)-IKZF1(2-4)-deimlink-NZF

    Plasmid
    #236174
    Purpose
    PiggyBac-integrated plasmid containing hygromycin resistance gene, ZNF35/IKZF1-driven BFP, and ZNF35/IKZF1-NZF transcription factor (with the IKZF1 portion containing ZFs 2-4 from that protein)
    Depositor
    Xiaojing Gao
    Article
    Wolfsberg et al bioRxiv 2025.02.24.639918
    Inserts
    ZNF35/IKZF1(2-4)-deimmunLink-NZF
    H2B (H2BC11 Human)
    Use
    Tags
    mTagBFP2
    Expression
    Mammalian
    Mutation
    Promoter
    CMV and four ZNF35/IKZF1 sites
    Available Since
    April 9, 2025
    Availability
    Academic Institutions and Nonprofits only
    236174-plasmid-map-sequence-id-468370

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  8. XZ393 (PB) EF1a-IL2Rbsp-FLAG-IL2Rb(ECD+TM)-TS-ADAR2dd-export-IRESv0-IL2Rgsp-3xHA-IL2Rg(ECD+TM)-TS-tdMCP-export

    Plasmid
    #233388
    Purpose
    PiggyBac donor plasmid with optimized IL-2-sensing eLIDAR receptor components separated by EMCV IRES, Puromycin resistance and BFP as marker
    Depositor
    Xiaojing Gao
    Article
    Zhang et al Nat Chem Biol. 2025 Mar 28. doi: 10.1038
    Insert
    IL2Rb(ecto+TM)-ADAR2dd
    Use
    Tags
    FLAG
    Expression
    Mammalian
    Mutation
    E488Q, T501A on ADAR2dd
    Promoter
    EF1a
    Available Since
    April 2, 2025
    Availability
    Academic Institutions and Nonprofits only
    233388-plasmid-map-sequence-id-467483

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  11. pPB9P3

    Plasmid
    #210034
    Purpose
    Contains cloning site for PE3/3b prime editing gRNA constructs, inducible prime editor 2, rtTA-T2A-puro
    Depositor
    Michael Talkowski
    Article
    Nuttle et al Cell Rep Methods. 2023 Dec 5:100672. doi
    Inserts
    prime editor 2
    rtta-T2A-puro
    Use
    CRISPR
    Tags
    SV40 NLS
    Expression
    Mammalian
    Mutation
    Promoter
    EF-1α and TRE3G
    Available Since
    Feb. 6, 2025
    Availability
    Academic Institutions and Nonprofits only
    210034-plasmid-map-sequence-id-414000

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  19. pPB-hTHp-Cre-pNeo

    Plasmid
    #214122
    Purpose
    Gene expression by Piggybac transposase in mammalian cells
    Depositor
    Sho Yoshimatsu
    Insert
    Cre
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Codon-optimized for mammalian cells
    Promoter
    Available Since
    Feb. 23, 2024
    Availability
    Academic Institutions and Nonprofits only
    214122-plasmid-map-sequence-id-423955

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  20. pPB-spCas9-pNeo

    Plasmid
    #214120
    Purpose
    Gene expression by Piggybac transposase in mammalian cells
    Depositor
    Sho Yoshimatsu
    Insert
    spCas9
    Use
    CRISPR
    Tags
    Expression
    Mammalian
    Mutation
    Codon-optimized for mammalian cells
    Promoter
    Available Since
    Feb. 22, 2024
    Availability
    Academic Institutions and Nonprofits only
    214120-plasmid-map-sequence-id-423617

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  21. pPB-spCas9-pZeo

    Plasmid
    #214121
    Purpose
    Gene expression by Piggybac transposase in mammalian cells
    Depositor
    Sho Yoshimatsu
    Insert
    spCas9
    Use
    CRISPR
    Tags
    Expression
    Mammalian
    Mutation
    Codon-optimized for mammalian cells
    Promoter
    Available Since
    Feb. 22, 2024
    Availability
    Academic Institutions and Nonprofits only
    214121-plasmid-map-sequence-id-423619

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  22. pPB-CAG-PlatTET-wpA-pPuroTK

    Plasmid
    #214125
    Purpose
    Gene expression by Piggybac transposase in mammalian cells
    Depositor
    Sho Yoshimatsu
    Insert
    dCas9-TET
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Codon-optimized for mammalian cells
    Promoter
    Available Since
    Feb. 27, 2024
    Availability
    Academic Institutions and Nonprofits only
    214125-plasmid-map-sequence-id-424237

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  23. BII-Sh-BnTW

    Plasmid
    #133365
    Purpose
    Piggybac vector for shRNA-mediated knockdown, with co-expression with tdTomato-NLS
    Depositor
    Blair Madison
    Insert
    tdTomato-NLS
    Use
    Tags
    Flag-tagged tdTomato
    Expression
    Mammalian
    Mutation
    N/A
    Promoter
    CMV enhancer and hEf1a (CpG free)
    Available Since
    Jan. 10, 2020
    Availability
    Academic Institutions and Nonprofits only
    133365-plasmid-map-sequence-id-268571

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  24. TK4-EGFP

    Plasmid
    #239047
    Purpose
    PiggyBac plasmid for stable transgene expression during human pluripotent stem cell differentiation
    Depositor
    Marius Wernig
    Inserts
    EGFP
    puroR
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CAG and EF1a
    Available Since
    May 21, 2025
    Availability
    Academic Institutions and Nonprofits only
    239047-plasmid-map-sequence-id-473421

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  25. pPB-CAG-dCas9-VPR-wpA-pPuroTK

    Plasmid
    #214127
    Purpose
    Gene expression by Piggybac transposase in mammalian cells
    Depositor
    Sho Yoshimatsu
    Insert
    dCas9-VPR
    Use
    CRISPR
    Tags
    Expression
    Mammalian
    Mutation
    Codon-optimized for mammalian cells
    Promoter
    Available Since
    Feb. 27, 2024
    Availability
    Academic Institutions and Nonprofits only
    214127-plasmid-map-sequence-id-424240

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  26. PB-TRE-dCas9-VPR-pA-3xsgRNA-EF1a-Tet.O-T2A-PuroR-polyA

    Plasmid
    #166693
    Purpose
    Express dCas9-VPR in a doxycyclin-inducable system and 3 sgRNAs targeting the murine Cnga1 promoter. Can be stably integrated into the genome via the PiggyBac Transposon system.
    Depositor
    Elvir Becirovic
    Article
    Bohm et al Sci Adv. 2020 Aug 19;6(34):eaba5614. doi
    Inserts
    dCas9-VPR
    3x Cnga1 promoter-targeting sgRNAs
    Use
    CRISPR
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    TRE and U6
    Available Since
    Feb. 1, 2023
    Availability
    Academic Institutions and Nonprofits only
    166693-plasmid-map-sequence-id-331043

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  27. PB-U6-CNCB_sgEIF3D-4

    Plasmid
    #236766
    Purpose
    A piggybac-based vector containing mouse U6 promoter-driven EIF3D sgRNA #4 and CAG promoter-driven nuclear-localized Clover-T2A-BSR.
    Depositor
    Kazutoshi Takahashi
    Article
    Okubo et al Sci Adv. 2025 Apr 11;11(15):eadq5484. do
    Insert
    EIF3D sgRNA-4 (EIF3D Human)
    Use
    Piggybac
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    mouse U6 promoter and mouse U6 promoter, CAG prom…
    Available Since
    May 22, 2025
    Availability
    Academic Institutions and Nonprofits only
    236766-plasmid-map-sequence-id-473592

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  28. pTRE-SOX17-P2A-Venus-rpEF1a-Zeo

    Plasmid
    #169174
    Purpose
    piggyBac transposon to generate a Tet inducible SOX17 expressing cell line. Tet-On inducible when paired with #169175
    Depositor
    Igor Slukvin
    Article
    Jung et al Cell Rep. 2021 Feb 16;34(7):108758. doi:
    Insert
    SOX17-P2A-Venus (SOX17 Synthetic, Human)
    Use
    Piggybac transposon
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    pTREtight (tet-inducible promoter)
    Available Since
    Jan. 21, 2022
    Availability
    Academic Institutions and Nonprofits only
    169174-plasmid-map-sequence-id-348368

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  32. PB-U6-CNCB_sgEIF3D-1

    Plasmid
    #236765
    Purpose
    A piggybac-based vector containing mouse U6 promoter-driven EIF3D sgRNA #1 and CAG promoter-driven nuclear-localized Clover-T2A-BSR.
    Depositor
    Kazutoshi Takahashi
    Article
    Okubo et al Sci Adv. 2025 Apr 11;11(15):eadq5484. do
    Insert
    EIF3D sgRNA-1 (EIF3D Human)
    Use
    Piggybac
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    mouse U6 promoter and mouse U6 promoter, CAG prom…
    Available Since
    July 8, 2025
    Availability
    Academic Institutions and Nonprofits only
    236765-plasmid-map-sequence-id-477909

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  33. PB-TRE3G-dN40-TP53-CCRB

    Plasmid
    #236774
    Purpose
    A piggybac-based cloning vector containing TRE3G promoter-driven short-form of TP53 and CAG promoter-driven nuclear-localized Clover-P2A-rtTA-IRES-BSD.
    Depositor
    Kazutoshi Takahashi
    Article
    Okubo et al Sci Adv. 2025 Apr 11;11(15):eadq5484. do
    Insert
    TP53 (TP53 Human)
    Use
    Piggybac
    Tags
    Expression
    Mammalian
    Mutation
    deleted amino acids 1-40
    Promoter
    TRE3G promoter and TRE3G promoter, CAG promoter
    Available Since
    May 29, 2025
    Availability
    Academic Institutions and Nonprofits only
    236774-plasmid-map-sequence-id-473566

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  34. PB-TRE3G-TP53-CCRB

    Plasmid
    #236773
    Purpose
    A piggybac-based cloning vector containing TRE3G promoter-driven full-length TP53 and CAG promoter-driven nuclear-localized Clover-P2A-rtTA-IRES-BSD.
    Depositor
    Kazutoshi Takahashi
    Article
    Okubo et al Sci Adv. 2025 Apr 11;11(15):eadq5484. do
    Insert
    TP53 (TP53 Human)
    Use
    Piggybac
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    TRE3G promoter and TRE3G promoter, CAG promoter
    Available Since
    May 29, 2025
    Availability
    Academic Institutions and Nonprofits only
    236773-plasmid-map-sequence-id-473536

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  35. XZ377 (PB) EF1a-VEGFR1sp-3xFlag-VEGFR1(ecto+TM)-TS-ADAR2dd(QA)-export-IRESv0-VEGFR2sp-HA-VEGFR2(Ecto+TM)-TS-tdMCP-export

    Plasmid
    #233385
    Purpose
    PiggyBac donor plasmid with optimized VEGF-sensing eLIDAR receptor components separated by EMCV IRES, Puromycin resistance and BFP as marker
    Depositor
    Xiaojing Gao
    Article
    Zhang et al Nat Chem Biol. 2025 Mar 28. doi: 10.1038
    Insert
    VEGFR1(ecto+TM)-ADAR2dd
    Use
    Tags
    FLAG, HA
    Expression
    Mammalian
    Mutation
    E488Q, T501A on ADAR2dd
    Promoter
    EF1a
    Available Since
    April 2, 2025
    Availability
    Academic Institutions and Nonprofits only
    233385-plasmid-map-sequence-id-467482

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  36. BII-gR-PnTW

    Plasmid
    #133356
    Purpose
    Piggybac vector for CRISPR/SpCas9 applications (nuclease, base editing, or epigenetic modifications) coexpressed with tdTomato-NLS
    Depositor
    Blair Madison
    Insert
    tdTomato-NLS
    Use
    Tags
    HA-tagged tdTomato
    Expression
    Mammalian
    Mutation
    N/A
    Promoter
    CMV enhancer and hEf1a (CpG free)
    Available Since
    Jan. 2, 2020
    Availability
    Academic Institutions and Nonprofits only
    133356-plasmid-map-sequence-id-267393

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  37. pB-CAG-PE2-NatMX

    Plasmid
    #158567
    Purpose
    Piggybac vector that allows for the generation of PE2-expressing stable cell lines
    Depositor
    Alejandro Chavez
    Insert
    PE2
    Use
    CRISPR
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available Since
    Aug. 28, 2020
    Availability
    Academic Institutions and Nonprofits only
    158567-plasmid-map-sequence-id-309882

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  38. PB-H2A-LSSmOrange2-2A-ERK-KTR-LSS-SGFP2

    Plasmid
    #129629
    Purpose
    Visualization of ERK activity
    Depositor
    Dorus Gadella
    Insert
    PB-H2A-LSSmOrange2-2A-ERK-KTR-LSS-SGFP2
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available Since
    April 1, 2025
    Availability
    Academic Institutions and Nonprofits only
    129629-plasmid-map-sequence-id-260251

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Showing: 441 - 480 of 1334 results