Addgene

Showing: 221 - 240 of 40798 results

Results per page:

Sort results by:

pRSFDuet-1_VNp-StefinA

Plasmid

#182403

Purpose

Bacterial expression of Vesicle Nucleating peptide-stefinA fusion

Depositor

Dan Mulvihill

Article

Eastwood et al Cell Rep Methods. 2023 Feb 2;3(2):100396

Insert

VNp-stefin (CSTA Human)

Use

Tags

Vesicle Nucleating peptide (VNp)

Expression

Bacterial

Mutation

Promoter

T7

Available since

Feb. 3, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pPOTv7-blast-mCardinal

Plasmid

#179796

Purpose

Trypanosoma brucei PCR only tagging (pPOT) vector for endogenous gene tagging

Depositor

Keith Gull, Samuel Dean

Article

Paterou et al Open Biol. 2025 Apr;15(4):240361. doi: 1

Insert

mCardinal

Use

Unspecified

Tags

Expression

Mutation

Promoter

read through

Available since

April 24, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pRS424-TUB1-Cterminal6xHis

Plasmid

#60387

Purpose

Expression of yeast alpha tubulin (TUB1)- Cterminal 6xHis using a GAL promoter

Depositor

Ron Vale

Article

Sirajuddin et al Nat Cell Biol. 2014 Apr;16(4):335-44. do

Insert

TUB1 (TUB1 Budding Yeast)

Use

Tags

6xHIS

Expression

Yeast

Mutation

Promoter

GAL

Available since

Jan. 26, 2015

Availability

Academic Institutions and Nonprofits only
CMV_Npu-saBE3.9max_KKH C-terminal

Plasmid

#137181

Purpose

Expresses the C-terminal split-intein fragment of S. aureus BE3.9max (KKH variant) from the CMV promoter

Depositor

David Liu

Article

Levy et al Nat Biomed Eng. 2020 Jan;4(1):97-110. do

Insert

Npu-saBE3.9max_KKH_C-terminal

Use

Tags

Expression

Mammalian

Mutation

E782K;N968K;R1015H conferring recognition of NNNR…

Promoter

CMV

Available since

Jan. 30, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
Human Mevalonate Kinase (MK)

Plasmid

#52429

Purpose

bacterial expression of human Mevalonate Kinase (MK)

Depositor

Henry Miziorko

Article

Potter et al J Biol Chem. 1997 Oct 10;272(41):25449-5

Insert

Human Mevalonate Kinase (MVK Human)

Use

Tags

Expression

Bacterial

Mutation

see comments

Promoter

T7

Available since

April 10, 2014

Availability

Academic Institutions and Nonprofits only
mCardinal-Alpha-Actinin-19

Plasmid

#56152

Purpose

Localization: Focal Adhesions / Actin, Excitation: 604, Emission: 659

Depositor

Michael Davidson

Article

Chu et al Nat Methods. 2014 Mar 16. doi: 10.1038/n

Insert

Alpha-Actinin (ACTN1 Human)

Use

Tags

mCardinal

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 27, 2015

Availability

Industry, Academic Institutions, and Nonprofits
pcDNA3-c-terminal mouseFc

Plasmid

#128024

Purpose

Mammalian expression vector with C terminal mFC tag

Depositor

Oskar Laur

Type

Empty backbone

Use

Tags

mFC

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 21, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
ninaE-stops myc

Plasmid

#26856

Depositor

Craig Montell

Article

Wang et al Neuron. 2008 Jan 10. 57(1):56-68.

Insert

Slow Termination of Phototransduction (stops Fly)

Use

Tags

myc

Expression

Insect

Mutation

Promoter

Available since

Jan. 5, 2011

Availability

Academic Institutions and Nonprofits only
pPTK012-3a-Inulinase-αMFΔ

Plasmid

#84971

Purpose

Type 3a, Inulinase followed by αMFΔ - secretion signal

Depositor

Volker Sieber

Article

Obst et al ACS Synth Biol. 2017 Mar 15. doi: 10.102

Insert

Inulinase followed by αMFΔ secretion tag

Use

Synthetic Biology

Tags

Expression

Yeast

Mutation

Promoter

Non

Available since

April 3, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pM-ErbB4-delta-kinase

Plasmid

#17800

Depositor

Marius Sudol

Article

Komuro et al J Biol Chem. 2003 Aug 29. 278(35):33334-

Insert

ErbB4 (ERBB4 Human)

Use

Tags

GAL4-BD

Expression

Mammalian

Mutation

Carboxy-terminal fragment, amino acids 988-1292, …

Promoter

Available since

May 9, 2008

Availability

Academic Institutions and Nonprofits only
pBS-germinal histone H4

Plasmid

#26600

Depositor

Phillip Newmark

Article

Wang et al Proc Natl Acad Sci U S A. 2007 Apr 3. 10

Insert

germinal histone H4

Use

Tags

Expression

Mutation

Promoter

Available since

Feb. 16, 2011

Availability

Academic Institutions and Nonprofits only
RNF168 C-terminal sgRNA

Plasmid

#207100

Purpose

pX330 based plasmid for expression of Cas9 and the GAGATGCACAAAGTAAGGCC sgRNA to target the RNF168 locus.

Depositor

Jens Schmidt

Article

Heyza et al Elife. 2023 Jun 21;12:e87086. doi: 10.75

Insert

GAGATGCACAAAGTAAGGCC

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV and U6

Available since

May 1, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
RIF C-terminal sgRNA

Plasmid

#207096

Purpose

pX330 based plasmid for expression of Cas9 and the AATACTAAATAGAATTTTCA sgRNA to target the RIF1 locus.

Depositor

Jens Schmidt

Article

Heyza et al Elife. 2023 Jun 21;12:e87086. doi: 10.75

Insert

AATACTAAATAGAATTTTCA

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV and U6

Available since

May 1, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
REV7 C-terminal sgRNA

Plasmid

#207095

Purpose

pX330 based plasmid for expression of Cas9 and the GCTCATAAAGGCAGCTGAGG sgRNA to target the REV7 locus.

Depositor

Jens Schmidt

Article

Heyza et al Elife. 2023 Jun 21;12:e87086. doi: 10.75

Insert

GCTCATAAAGGCAGCTGAGG

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV and U6

Available since

May 1, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
N-Terminal Halo XRCC4

Plasmid

#207541

Purpose

Homologous recombination donor to insert halo tag at the N terminal of the endogenous XRCC4 locus.

Depositor

Jens Schmidt

Article

Mikhova et al Nat Commun. 2024 Nov 23;15(1):10159. doi

Insert

HaloTag with internal PuroR cassette flanked by human XRCC4 locus sequences

Use

Tags

HaloTag

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 16, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
N-terminal Halo-Ku70

Plasmid

#207547

Purpose

Homologous recombination donor to insert halo tag at the N terminal of the endogenous Ku70 locus.

Depositor

Jens Schmidt

Article

Mikhova et al Nat Commun. 2024 Nov 23;15(1):10159. doi

Insert

HaloTag with flanked by human Ku70 locus sequences

Use

Tags

HaloTag

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 16, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
SAM-DNMT3A-inactiveEmpty

Plasmid

#213168

Purpose

Empty Vector (no sgRNA) used as a negative control (inactive DNMT3A) for induction of global DNA methylation.

Depositor

Sefi Rosenbluh

Article

Hosseinpour et al Nat Commun. 2024 Dec 1;15(1):10449. doi:

Type

Empty backbone

Use

CRISPR, Lentiviral, and Synthetic Biology

Tags

Expression

Mammalian

Mutation

Promoter

E1Fa and U6

Available since

Dec. 2, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pHAGE-mCardinal-SKL

Plasmid

#183728

Purpose

For exrpression of mCardinal targeted to peroxisomes

Depositor

Jeffrey Gerst

Article

Haimovich et al bioRxiv 2024.11.06.622258

Insert

mCardinal

Use

Lentiviral

Tags

SKL (peroxisomal targeting sequence 1)

Expression

Mutation

Promoter

UbiC

Available since

Nov. 11, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
SHLD3 N-terminal sgRNA

Plasmid

#207094

Purpose

pX330 based plasmid for expression of Cas9 and the TTACTGCAGAATGACTACAG sgRNA to target the SHLD3 locus.

Depositor

Jens Schmidt

Article

Heyza et al Elife. 2023 Jun 21;12:e87086. doi: 10.75

Insert

TTACTGCAGAATGACTACAG

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV and U6

Available since

May 6, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
NBS1 C-terminal sgRNA

Plasmid

#207092

Purpose

pX330 based plasmid for expression of Cas9 and the TAAAAAGGAGAAGATAACTG sgRNA to target the NBS1 locus.

Depositor

Jens Schmidt

Article

Heyza et al Elife. 2023 Jun 21;12:e87086. doi: 10.75

Insert

TAAAAAGGAGAAGATAACTG

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV and U6

Available since

May 6, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge