We narrowed to 15,661 results for: genome
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Plasmid#91158PurposeCas9 only expression, Cas9 Type: AtCas9, Plant Selection: 2x35S:barDepositorInsertAtCas9
UseCRISPRExpressionPlantAvailable SinceJuly 10, 2017AvailabilityAcademic Institutions and Nonprofits only -
pYPQ262m3
Plasmid#149556PurposeGateway entry clone for CRISPR-iSpyMacCas9 ABE7.10 A-G base editing at NAAR PAMsDepositorInsertecTadAwt-ecTadAmut-ziSpyMacCas9(D10A)
UseCRISPRExpressionPlantMutationD10A, R221K, N394K, Protospacer interacting domai…Available SinceJuly 20, 2020AvailabilityAcademic Institutions and Nonprofits only -
pGEX2TK-Num1-PH
Plasmid#71369Purposebacterial expression of PH domain for phosphoinositide binding assayDepositorInsertNum1-PH
TagsGSTExpressionBacterialMutationPleckstrin homology (PH) domain; aa2563-2692PromotertacAvailable SinceFeb. 26, 2016AvailabilityAcademic Institutions and Nonprofits only -
LbCas12a
Plasmid#188493PurposeExpresses FLAG tagged LbCas12aDepositorInsertCas12a
UseCRISPR and LentiviralExpressionMammalianPromoterEF1aAvailable SinceSept. 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
pMOD_A0901
Plasmid#91025PurposeModule A, Promoter: 35S, Gene: TREX2-P2A-AtCas9, Terminator: HSPDepositorInsertTREX2-P2A-AtCas9
UseCRISPRPromoter35SAvailable SinceJuly 7, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCfB9336 (pgRNA_II-1_NatMX)
Plasmid#161589PurposeEasyClone-MarkerFree guiding RNA vector to direct Cas9 to cut at site II-1DepositorInsertguiding RNA
UseCRISPR and Synthetic BiologyExpressionYeastAvailable SinceAug. 9, 2022AvailabilityAcademic Institutions and Nonprofits only -
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pBS-KS-attB1-2-PT-SA-SD-2-2xTY1-V5
Plasmid#61257PurposeCRISPR-RMCE, second step attB plasmid, intron phase 2, exchange dsRed with 2xTY1 and V5 tags cassetteDepositorInsert2xTY1-V5
UseCRISPRTagsTY1 and V5Available SinceNov. 20, 2014AvailabilityAcademic Institutions and Nonprofits only -
2X_pX458_pSpCas9(BB)-2A-GFP_SOX17_bKO
Plasmid#172225PurposeCas9-2A-GFP expression vector bearing two sgRNAs targeting the centromeric human SOX17 CTCF-boundary.DepositorInsertsgRNA
UseCRISPRTags3XFLAG and GFPExpressionMammalianPromoterCbhAvailable SinceSept. 20, 2021AvailabilityAcademic Institutions and Nonprofits only -
pMLS791
Plasmid#188889PurposeTargeting vector for insertion into 5' miniMos Arm; carries floxed Cbr-unc-119 marker, Phsp16.41::Cre and Pmyo-2::2xNLS::cyOFPDepositorInsertfloxed Cbr-unc-119 marker, Phsp16.41::Cre and Pmyo-2::2xNLS::cyOFP
ExpressionWormMutationC. elegans codom optimization and intron additionAvailable SinceAug. 30, 2022AvailabilityAcademic Institutions and Nonprofits only -
T5-sgRNA
Plasmid#113655PurposesgRNA targeting unit plasmid. The sgRNA targeting unit plasmids contain connector ConLS, ConR1, and one sgRNA transcriptional unit.DepositorInsertpromoter T5 and sgRNA
UseCRISPRExpressionBacterialPromoterT5Available SinceSept. 18, 2018AvailabilityAcademic Institutions and Nonprofits only -
pRvCAHS3-mEGFP
Plasmid#205008PurposeThe vector contains 1kbp of the upstream and downstream regions of the cahs3 gene from Ramazzottius varieornatus, with the mEGFP gene positioned in between.DepositorInsertmEGFP flanked by 1kbp upstream and downstream of the cahs3 gene from Ramazzottius varieornatus
UseTardigrade expressionAvailable SinceAug. 22, 2023AvailabilityAcademic Institutions and Nonprofits only -
pRvACT-mCherry
Plasmid#205002PurposeThe vector contains 1kbp of the upstream and downstream regions of the actin gene from Ramazzottius varieornatus, with the mCherry gene with NLS sequence positioned in between.DepositorInsertmCherry-NLS flanked by 1kbp upstream and downstream of the actin gene from R. varieornatus
UseTardigrade expressionAvailable SinceAug. 21, 2023AvailabilityAcademic Institutions and Nonprofits only -
pMOD_A0508
Plasmid#91014PurposeModule A, Promoter: EC1.2, Gene: Csy4-P2A-AtCas9, Terminator: HSPDepositorInsertCsy4-P2A-AtCas9
UseCRISPRPromoterEC1.2Available SinceJuly 7, 2017AvailabilityAcademic Institutions and Nonprofits only -
pEPOR1CB0013
Plasmid#117549PurposeLevel 1 Golden Gate Cassette: LbCas12a expression cassette for dicotyledonous plantsDepositorInsert2x35S+5'UTR OMEGA (pICH51288) + LbCas12a (with stop codon) (pEPOR0SP0007)+ Nos (pICH41421)
ExpressionPlantPromoter2x35S+5'UTR OMEGA (pICH51288)Available SinceNov. 13, 2018AvailabilityAcademic Institutions and Nonprofits only -
pRS426-GFP-Osh1-PH
Plasmid#71372PurposeFor analysis of PH domain localization in yeastDepositorInsertOsh1-PH
TagsEGFPExpressionYeastMutationPleckstrin homology (PH) domain; aa267-388Available SinceFeb. 26, 2016AvailabilityAcademic Institutions and Nonprofits only -
pGEX2TK-Skm1-PH
Plasmid#71371Purposebacterial expression of PH domain for phosphoinositide binding assayDepositorInsertSkm1-PH
TagsGSTExpressionBacterialMutationPleckstrin homology (PH) domain; aa1-132PromotertacAvailable SinceFeb. 26, 2016AvailabilityAcademic Institutions and Nonprofits only -
pBS448 RSV-GFPcre
Plasmid#11919PurposeExpresses Cre-GFP fusion under the control of the Rous sarcoma virus (RSV) LTR/promoterDepositorAvailable SinceSept. 22, 2006AvailabilityAcademic Institutions and Nonprofits only -
plYJP067-Ptet
Plasmid#202299PurposePTet-YFP plasmid for LPattB2 integrationDepositorInsertPtet-YFP
ExpressionBacterialAvailable SinceSept. 18, 2023AvailabilityAcademic Institutions and Nonprofits only -
pBA1032
Plasmid#189580PurposeT4soc deletion HR vector 250bp HR Arms (T4 phage editing)DepositorInsertT4soc Deletion Locus 250bp Homology Arms
UseUnspecifiedMutationEncodes for a full deletion of T4socAvailable SinceAug. 29, 2022AvailabilityAcademic Institutions and Nonprofits only -
px330 p300 gRNA
Plasmid#165591PurposeInsertion of p300 degronDepositorAvailable SinceMarch 12, 2021AvailabilityAcademic Institutions and Nonprofits only -
pMJ915v2+Nterm Spe1 +Cterm Age1 site
Plasmid#88914PurposeFor expression of modified SpyCas9 in e.coli.DepositorTypeEmpty backboneUseCRISPRExpressionBacterialAvailable SinceMay 2, 2017AvailabilityAcademic Institutions and Nonprofits only -
psgRNA2.02-PP7-ccdB
Plasmid#99912PurposeBasic cloning vector for the expression of PP7 inserted sgRNADepositorTypeEmpty backboneUseCRISPRExpressionMammalianAvailable SinceOct. 10, 2017AvailabilityAcademic Institutions and Nonprofits only -
pHiUGE V5-epitope donor ORF0
Plasmid#200377PurposeTagging endogenously expressed proteins with V5 epitope, C-terminalDepositorInsertV5-epitope
ExpressionMammalianMutationNAAvailable SinceJuly 10, 2023AvailabilityAcademic Institutions and Nonprofits only -
pQTEV-VPS28
Plasmid#34803DepositorAvailable SinceMarch 25, 2013AvailabilityAcademic Institutions and Nonprofits only -
px330 RAD21 gRNA
Plasmid#165592PurposeInsertion of RAD21 degronDepositorAvailable SinceMarch 12, 2021AvailabilityAcademic Institutions and Nonprofits only -
psgRNA2.02-MS2-ccdB
Plasmid#99911PurposeBasic cloning vector for the expression of MS2 inserted sgRNADepositorTypeEmpty backboneUseCRISPRExpressionMammalianAvailable SinceOct. 10, 2017AvailabilityAcademic Institutions and Nonprofits only -
pAJT430
Plasmid#202291PurposeRPU integration plasmid (LPattB7)DepositorInsertJ23101-RiboJ00-B0034-YFP
ExpressionBacterialAvailable SinceSept. 18, 2023AvailabilityAcademic Institutions and Nonprofits only -
PGL3-MK17
Plasmid#21486DepositorAvailable SinceJuly 23, 2009AvailabilityAcademic Institutions and Nonprofits only -
pQTEV-TPT1
Plasmid#31558DepositorInserttumor protein, translationally-controlled 1 (TPT1 Human)
TagsHis and TEVExpressionBacterialAvailable SinceMarch 22, 2013AvailabilityAcademic Institutions and Nonprofits only -
pMOD_A0908
Plasmid#91027PurposeModule A, Promoter: EC1.2, Gene: TREX2-P2A-AtCas9, Terminator: HSPDepositorInsertTREX2-P2A-AtCas9
UseCRISPRPromoterEC1.2Available SinceAug. 21, 2017AvailabilityAcademic Institutions and Nonprofits only -
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pAJT254-litR
Plasmid#202308PurposelitR repressor plasmid for LPattB2 integrationDepositorInsertlitR
ExpressionBacterialAvailable SinceSept. 18, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAJT249-psrA
Plasmid#202304PurposepsrA repressor plasmid for LPattB2 integrationDepositorInsertpsrA
ExpressionBacterialAvailable SinceSept. 18, 2023AvailabilityAcademic Institutions and Nonprofits only -
EFS eGFP hPGK BSD
Plasmid#188520PurposeBackbone for CRISPR interference (CRISPRi) isogenic PAM testingDepositorTypeEmpty backboneUseLentiviralTagseGFPExpressionMammalianAvailable SinceSept. 29, 2022AvailabilityAcademic Institutions and Nonprofits only -
pGEM-sgMUC4_dual-MTS-3x-Set I
Plasmid#162762PurposeSeparately expressing 3 unique sgMUC4_dual-MTS (SetI)DepositorInsertsgMUC4_dual-MTS_1; sgMUC4_dual-MTS_2; sgMUC4_dual-MTS_3
ExpressionMammalianPromoterU6Available SinceJan. 6, 2021AvailabilityAcademic Institutions and Nonprofits only -
pTZ tRNA_gRNA empty scaffold
Plasmid#188450PurposeExpression of gRNAs using a human tRNA promoterDepositorTypeEmpty backboneUseCRISPRExpressionMammalianPromotertRNA glutamineAvailable SinceSept. 21, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCF730
Plasmid#121668PurposeEF1a-ProCas9-Flavi-P2A-PuroDepositorInsertCas9(C)
UseLentiviralPromoterEF-1a core promoterAvailable SinceMarch 5, 2019AvailabilityAcademic Institutions and Nonprofits only -
pBS-KS-attB1-2-PT-SA-SD-1-2xTY1-V5
Plasmid#61256PurposeCRISPR-RMCE, second step attB plasmid, intron phase 1, exchange dsRed with 2xTY1 and V5 tags cassetteDepositorInsert2xTY1-V5
UseCRISPRTagsTY1 and V5Available SinceNov. 20, 2014AvailabilityAcademic Institutions and Nonprofits only