Addgene

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Showing: 5951 - 6000 of 32142 results

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pNY-351

Plasmid

#231048

Purpose

Used for staining GFP

Depositor

Jeremy Nathans

Insert

anti GFP DARPin fused to goat Fc

Use

Affinity Reagent/ Antibody

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 17, 2025

Availability

Academic Institutions and Nonprofits only

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pHDR_DDX4-T2A-tdTomato-puroTK

Plasmid

#222519

Purpose

Homology directed repair template for knocking in tdTomato reporter to DDX4.

Depositor

George Church

Article

Pierson Smela et al bioRxiv 2024.05.31.596483

Insert

tdTomato

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

Jan. 27, 2025

Availability

Academic Institutions and Nonprofits only

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pHDR_REC8-T2A-mGreenLantern-puroTK

Plasmid

#222521

Purpose

Homology directed repair template for knocking in mGreenLantern reporter to REC8.

Depositor

George Church

Article

Pierson Smela et al bioRxiv 2024.05.31.596483

Insert

mGreenLantern

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

Jan. 27, 2025

Availability

Academic Institutions and Nonprofits only

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p42.3A7

Plasmid

#69575

Purpose

OT-I TCR a chain cDNA in pCR1000

Depositor

Francis R. Carbone

Article

Hogquist et al Cell. 1994 Jan 14;76(1):17-27.

Insert

TCR alpha clone 149.42 (Tcra Mouse)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Sept. 30, 2015

Availability

Academic Institutions and Nonprofits only

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pJA252

Plasmid

#21512

Depositor

Julie Ahringer

Article

Zeiser et al PLoS One. 2011;6(5):e20082. Epub 2011 Ma

Insert

pmex-5 (w/o ATG)

Use

Multisite gateway

Tags

Expression

Mutation

Promoter

Available since

Aug. 11, 2009

Availability

Academic Institutions and Nonprofits only

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RV-L3-HyTK-2L

Plasmid

#11684

Depositor

Geoff Wahl

Article

Wong et al Nucleic Acids Res. 2005 . 33(17):e147.

Type

Empty backbone

Use

Cre/Lox and Retroviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

May 4, 2006

Availability

Academic Institutions and Nonprofits only

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pJRT.Agro.CLCrVB1.3

Plasmid

#37974

Depositor

Niki Robertson

Article

Tuttle et al Plant Methods. 2012 Aug 1;8(1):27. doi:

Insert

pJRTCLCrVB1.3

Use

Agrobacterium binary

Tags

Expression

Mutation

Promoter

Available since

Oct. 15, 2012

Availability

Academic Institutions and Nonprofits only

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pLentiN

Plasmid

#37444

Purpose

Control lentiviral plasmid containing FLAG-HA tags

Depositor

Karl Munger

Article

Spangle et al J Virol. 2012 May 2.

Insert

FLAG-HA

Use

Lentiviral

Tags

Flag and HA

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Aug. 3, 2012

Availability

Academic Institutions and Nonprofits only
pMRX-IZ-HaloTag7-Galectin3

Plasmid

#184896

Purpose

Stable expression of HaloTag7-Galectin3 in mammalian cells

Depositor

Noboru Mizushima

Article

Yim et al FEBS Lett. 2022 Mar 11. doi: 10.1002/187

Insert

Galectin3

Use

Tags

HaloTag7

Expression

Mammalian

Mutation

Promoter

Available since

June 13, 2022

Availability

Academic Institutions and Nonprofits only

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p4053 HA-E6AP isoform I

Plasmid

#8657

Depositor

Peter Howley

Article

Kao et al J Virol 2000 Jul;74(14):6408-17.

Insert

E6-AP isoform I (UBE3A Human)

Use

Tags

HA

Expression

Mammalian

Mutation

Promoter

CMV

Available since

June 16, 2005

Availability

Academic Institutions and Nonprofits only

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pMApra-Ptac

Plasmid

#218357

Purpose

Empty vector with lacIq-Ptac expression system

Depositor

Michael Gebhardt

Article

Intorcia et al Appl Environ Microbiol. 2024 Sep 18;90(9

Type

Empty backbone

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

May 2, 2024

Availability

Academic Institutions and Nonprofits only

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pMK433 (mAID-P2A-BSD)

Plasmid

#168228

Purpose

C-terminal tagging with mAID

Depositor

Masato Kanemaki

Article

Saito et al Curr Protoc. 2021 Aug;1(8):e219. doi: 10

Insert

mAID-P2A-BSD

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 21, 2022

Availability

Academic Institutions and Nonprofits only

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PA-GFPmt

Plasmid

#19989

Depositor

Orian Shirihai

Article

Twig et al Am J Physiol Cell Physiol. 2006 Jul . 29

Insert

GFP

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

Sept. 17, 2009

Availability

Academic Institutions and Nonprofits only
pMK432 (mAID-P2A-Hygro)

Plasmid

#168227

Purpose

C-terminal tagging with mAID

Depositor

Masato Kanemaki

Article

Saito et al Curr Protoc. 2021 Aug;1(8):e219. doi: 10

Insert

mAID-P2A-Hygro

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 21, 2022

Availability

Academic Institutions and Nonprofits only

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PaGFP-UtrCH

Plasmid

#26738

Depositor

William Bement

Article

Burkel et al Cell Motil Cytoskeleton. 2007 Nov . 64(1

Insert

Utrophin

Use

Reporter for f-actin

Tags

photoactivatable GFP

Expression

Mammalian

Mutation

Calponin homology domain of utrophin (aa# 1-261)

Promoter

Available since

Feb. 24, 2011

Availability

Academic Institutions and Nonprofits only

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mRFP-UtrCH

Plasmid

#26739

Depositor

William Bement

Article

Burkel et al Cell Motil Cytoskeleton. 2007 Nov . 64(1

Insert

Utrophin

Use

Reporter for f-actin

Tags

monomeric RFP

Expression

Mammalian

Mutation

Calponin homology domain of utrophin (aa# 1-261)

Promoter

Available since

Feb. 24, 2011

Availability

Academic Institutions and Nonprofits only

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pGEX-2TK 14-3-3 sigma GST

Plasmid

#11944

Depositor

Michael Yaffe

Article

Yaffe et al Cell. 1997 Dec 26. 91(7):961-71.

Insert

14-3-3 sigma (SFN Human)

Use

Tags

GST

Expression

Bacterial

Mutation

Promoter

Available since

May 17, 2006

Availability

Academic Institutions and Nonprofits only
pMXs-Tcl1

Plasmid

#13364

Depositor

Shinya Yamanaka

Article

Takahashi et al Cell. 2006 Aug 25. 126(4):663-76.

Insert

T-cell lymphoma breakpoint 1 (Tcl1 Mouse)

Use

Retroviral

Tags

Expression

Mammalian

Mutation

None

Promoter

Available since

Jan. 12, 2007

Availability

Academic Institutions and Nonprofits only
pRK5 HA GST Rheb1

Plasmid

#14951

Depositor

David Sabatini

Article

Sancak et al Mol Cell. 2007 Mar 23. 25(6):903-15.

Insert

Rheb1 (RHEB Human)

Use

Tags

HA-GST

Expression

Mammalian

Mutation

Promoter

Available since

May 10, 2007

Availability

Academic Institutions and Nonprofits only

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pXWS40tetO

Plasmid

#160827

Purpose

40 repeats of tetO sponge

Depositor

Baojun Wang

Article

Wan et al Nat Commun. 2020 Nov 24;11(1):5961. doi:

Insert

(40 X tetO)-t

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

Nov. 19, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pWS3867

Plasmid

#185744

Purpose

Sub-array backbone 3/4

Depositor

Rodrigo Ledesma-Amaro

Article

Shaw et al Nat Commun. 2022 Aug 25;13(1):4984. doi:

Type

Empty backbone

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

Oct. 28, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pBS-pIgR cDNA

Plasmid

#12109

Depositor

Pamela Bjorkman

Article

Hamburger et al Structure. 2004 Nov . 12(11):1925-35.

Insert

human polymeric immunoglobulin receptor (PIGR Human)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Aug. 4, 2006

Availability

Academic Institutions and Nonprofits only
pSSa29

Plasmid

#179503

Purpose

Tet-on red-shifted luciferase expression

Depositor

Dominique Sanglard

Article

Schrevens et al FEMS Yeast Res. 2022 Sep 24;22(1):foac04

Insert

Red-shifted luciferase

Use

Tags

Expression

Mutation

Promoter

Tet-HOP1 (p98CGH)

Available since

Feb. 15, 2022

Availability

Academic Institutions and Nonprofits only

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iDuet101a

Plasmid

#17629

Depositor

Linzhao Cheng

Article

Zhou et al Stem Cells. 2007 Mar . 25(3):779-89.

Insert

TetO7 - EF-1a promoter - GFP - PGK promoter - Hygro

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 27, 2008

Availability

Academic Institutions and Nonprofits only

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pTK503

Plasmid

#114717

Purpose

Express mCherry-NuMA(WT) from Rosa 26 locus

Depositor

Tomomi Kiyomitsu

Article

Okumura et al Elife. 2018 May 31;7. pii: 36559. doi: 1

Insert

NuMA

Use

Tags

Expression

Mutation

Promoter

Available since

April 11, 2019

Availability

Academic Institutions and Nonprofits only

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pET28a-SUMO-SpyTag003

Plasmid

#216282

Purpose

Expresses SUMO with a C-terminal SpyTag003 in bacterial cells

Depositor

Mark Howarth

Article

Driscoll et al Nat Commun. 2024 Mar 16;15(1):2403. doi:

Insert

SUMO-SpyTag003

Use

Tags

His6 and SpyTag003

Expression

Bacterial

Mutation

Promoter

T7 promoter

Available since

June 26, 2024

Availability

Academic Institutions and Nonprofits only

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pHCD1A

Plasmid

#102952

Purpose

gene cloning of human CD1A

Depositor

Lucia Mori, Gennaro De Libero

Article

Manolova et al Eur J Immunol. 2003 Jan;33(1):29-37.

Insert

CD1A cDNA (CD1A Human)

Use

Cloning

Tags

Expression

Mutation

Promoter

Available since

Dec. 12, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pGEX-4T1-14-3-3 zeta GST

Plasmid

#13278

Depositor

Michael Yaffe

Article

Yaffe et al Cell. 1997 Dec 26. 91(7):961-71.

Insert

14-3-3 zeta (YWHAZ Bovine)

Use

Tags

GST

Expression

Bacterial

Mutation

Promoter

Available since

Oct. 7, 2006

Availability

Academic Institutions and Nonprofits only
pMOTag-glmS-4H

Plasmid

#106378

Purpose

Can be used as a PCR template to amplify the glmS-HA fragment for gene specific tagging and regulation

Depositor

Roberto Docampo

Article

Cruz-Bustos et al J Eukaryot Microbiol. 2017 Dec 19. doi:

Insert

GlmS-3xHA

Use

Tags

Expression

Mutation

Promoter

Available since

March 2, 2018

Availability

Industry, Academic Institutions, and Nonprofits

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pCB6-EnvA

Plasmid

#74420

Purpose

Mammalian expression of the subgroup A avian leukosis-sarcoma envelope glycoprotein (EnvA)

Depositor

Judith White

Article

Gilbert et al J Virol. 1994 Sep;68(9):5623-8.

Insert

Env from ASLV-A

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Aug. 27, 2018

Availability

Academic Institutions and Nonprofits only
pBS Sp1 full length

Plasmid

#12096

Depositor

Robert Tjian

Article

Kadonaga et al Cell. 1987 Dec 24. 51(6):1079-90.

Insert

Sp1 (SP1 Human)

Use

Cloning vector

Tags

Expression

Mutation

Promoter

Available since

Aug. 3, 2006

Availability

Academic Institutions and Nonprofits only
pWS3868

Plasmid

#185745

Purpose

Sub-array backbone 4/4

Depositor

Rodrigo Ledesma-Amaro

Article

Shaw et al Nat Commun. 2022 Aug 25;13(1):4984. doi:

Type

Empty backbone

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

Oct. 28, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pHR'CMVGFP_NLS_hRIF1(1-1308)

Plasmid

#23134

Depositor

Lifeng Xu

Article

Xu et al J Cell Biol. 2004 Dec 6. 167(5):819-30.

Insert

RIF1 deletion construct (RIF1 Human)

Use

Lentiviral

Tags

GFP

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 17, 2010

Availability

Academic Institutions and Nonprofits only

Enlarge
pJJH1320

Plasmid

#36915

Depositor

Jürgen Heinisch

Article

Bakota et al Mol Genet Genomics. 2012 Apr;287(4):313-

Insert

Cre recombinase

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

July 17, 2012

Availability

Academic Institutions and Nonprofits only

Enlarge
pQE60-Exp5

Plasmid

#12553

Depositor

Ian Macara

Article

Brownawell et al J Cell Biol. 2002 Jan 7. 156(1):53-64.

Insert

EXPORTIN-5 (XPO5 Human)

Use

Tags

HIS

Expression

Bacterial

Mutation

NONE

Promoter

Available since

Oct. 2, 2006

Availability

Academic Institutions and Nonprofits only
pL3-TRE-MCS-polyA-2L

Plasmid

#11719

Depositor

Geoff Wahl

Article

Wong et al Nucleic Acids Res. 2005 . 33(17):e147.

Type

Empty backbone

Use

Cre/Lox

Tags

Expression

Mammalian

Mutation

Promoter

Available since

May 4, 2006

Availability

Academic Institutions and Nonprofits only

Enlarge
PJEBAN2

Plasmid

#203681

Purpose

Expresses Cyan Fluorescent Protein (CFP) in Listeria monocytogenes

Depositor

Tine Rask Licht

Article

Andersen et al BMC Microbiol. 2006 Oct 3;6:86. doi: 10.

Insert

Cyan Fluorescent Protein (CFP)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Sept. 20, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pTK630

Plasmid

#114697

Purpose

Express RFP-Nano from Rosa 26 locus

Depositor

Tomomi Kiyomitsu

Article

Okumura et al Elife. 2018 May 31;7. pii: 36559. doi: 1

Insert

RFP-Nano

Use

Tags

Expression

Mutation

Promoter

Available since

Oct. 16, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
cmv-mCherry-8xPP7

Plasmid

#185795

Purpose

mCherry labeled with 8xPP7

Depositor

Robin Lee

Article

Guo et al Cell Rep Methods. 2022 May 25;2(6):10022

Insert

cmv-mCherry-8xPP7

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

July 18, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pJW1663

Plasmid

#112037

Purpose

Gal-Estradiol transcription activation expression cassette marked with KanR

Depositor

Jonathan Weissman

Article

Costa et al Science. 2018 Jan 18. pii: science.aar36

Insert

Gal DNA binding-estradiol binding-MSN2 activator

Use

Tags

Expression

Yeast

Mutation

Promoter

ADH1

Available since

March 22, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3.1 FLIPglu-600uDelta13V

Plasmid

#18014

Depositor

Wolf Frommer

Article

Takanaga et al FASEB J. 2010 Aug . 24(8):2849-58.

Insert

FLIPglu F16A Delta13

Use

Tags

ECFP and Venus

Expression

Mammalian

Mutation

F16A in binding protein

Promoter

Available since

Feb. 17, 2011

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3.1(-)-Cygnet2.1

Plasmid

#19737

Depositor

Wolfgang Dostmann

Article

Honda et al Proc Natl Acad Sci U S A. 2001 Feb 27. 9

Insert

Cygnet 2.1

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 27, 2008

Availability

Academic Institutions and Nonprofits only
pXWS320tetO

Plasmid

#160830

Purpose

320 repeats of tetO sponge

Depositor

Baojun Wang

Article

Wan et al Nat Commun. 2020 Nov 24;11(1):5961. doi:

Insert

(320 X tetO)-t

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

Nov. 19, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pMOD_D0000a

Plasmid

#127230

Purpose

Empty Backbone for golden gate cloning compatible with Cermak., et al Toolkit.

Depositor

Daniel Voytas

Article

Maher et al Nat Biotechnol. 2019 Dec 16. pii: 10.103

Type

Empty backbone

Use

Tags

Expression

Plant

Mutation

Promoter

Available since

Dec. 17, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pXWS160tetO

Plasmid

#160829

Purpose

160 repeats of tetO sponge

Depositor

Baojun Wang

Article

Wan et al Nat Commun. 2020 Nov 24;11(1):5961. doi:

Insert

(160 X tetO)-t

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

Nov. 19, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pCMV-UnaG-pA (pE091)

Plasmid

#149354

Purpose

Mammalian Expression vector for UnaG

Depositor

Ralf Kuehn

Article

Danner et al Open Biol. 2021 Jan;11(1):200283. doi: 1

Insert

UnaG

Use

Tags

Expression

Mammalian

Mutation

Promoter

CAG

Available since

Jan. 27, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
sZF15

Plasmid

#43846

Depositor

George Church

Article

Mali et al Nat Methods. 2013 Mar 17. doi: 10.1038/n

Insert

sZF15

Use

Tags

HA Flag and Myc

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 11, 2013

Availability

Academic Institutions and Nonprofits only
pTol2-DRv7

Plasmid

#78627

Purpose

GESTALT V7 barcode for zebrafish

Depositor

Jay Shendure

Article

McKenna et al Science. 2016 May 26. pii: aaf7907.

Insert

GESTALT lineage tracing target V7

Use

Tags

Expression

Mutation

Promoter

Available since

July 12, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pXWS80tetO

Plasmid

#160828

Purpose

80 repeat of tetO sponge

Depositor

Baojun Wang

Article

Wan et al Nat Commun. 2020 Nov 24;11(1):5961. doi:

Insert

(80 X tetO)-t

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

Nov. 19, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
HA.PKD.Y463E

Plasmid

#10812

Depositor

Alex Toker

Article

Storz et al EMBO J. 2003 Jan 2. 22(1):109-20.

Insert

PKD (PRKD1 Human)

Use

Tags

HA

Expression

Mammalian

Mutation

Y463E--active; P467L and L520P

Promoter

CMV

Available since

Oct. 6, 2005

Availability

Academic Institutions and Nonprofits only

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