We narrowed to 42,011 results for: LAS

Showing: 2381 - 2400 of 42011 results

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pLAS3W-S100P

Plasmid

#125009

Purpose

express in mammalian cell

Depositor

Ruey-Hwa Chen

Article

Tan et al Oncogene. 2019 Jul;38(28):5612-5626. doi

Insert

S100P (S100P Human)

Expression

Mammalian

Promoter

CAG

Available Since

April 1, 2022

Availability

Academic Institutions and Nonprofits only

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Plas-CRISPR_reporter

Plasmid

#157658

Purpose

CRISPR reporter for mRNA quantification, integrative plasmid into NPR2 gene

Depositor

Frank Albert

Article

Brion et al Elife. 2020 Nov 16;9. pii: 60645. doi: 1

Insert

dCAS9-VP64, mCherry, KanMX

Use

Insert storage (replicative in e. coli)

Mutation

N28D in mCherry- please see depositor comments be…

Available Since

Sept. 15, 2020

Availability

Academic Institutions and Nonprofits only

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pAdTrack shALAS-1

Plasmid

#22748

Depositor

Bruce Spiegelman

Article

Estall et al Proc Natl Acad Sci U S A. 2009 Dec 29. 1

Insert

shALAS-1 (Alas1 Mouse)

Use

Adenoviral and RNAi

Expression

Mammalian

Available Since

July 14, 2010

Availability

Academic Institutions and Nonprofits only
p2T-cmv-SpCas9-TadCBEd-V106W-BlastR

Plasmid

#193849

Purpose

Express TadCBEd-V106W in mammalian cells with BlastR selection, Tol2 integration

Depositor

David Liu

Article

Neugebauer et al Nat Biotechnol. 2022 Nov 10. pii: 10.103

Insert

TadCBEd-V106W

Expression

Mammalian

Available Since

Dec. 15, 2022

Availability

Academic Institutions and Nonprofits only

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qTAG-AAVS1-Ef1a-Blast

Plasmid

#227267

Purpose

Empty AAVS1 targeting donor for the insertion of Blast and a strong EF1a promoter. A CDS can be cloned adjacent to the promoter via restriction or gibson cloning using the MluI cut site.

Depositor

Laurence Pelletier

Article

Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

Type

Empty backbone

Use

CRISPR; Donor cassette

Expression

Mammalian

Available Since

Nov. 12, 2024

Availability

Academic Institutions and Nonprofits only

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pCW57.1 blast tBID-flag

Plasmid

#211533

Purpose

Expresses truncated BID upon doxycycline addition to trigger apoptosis

Depositor

Richard Possemato

Article

Nam et al Nat Metab. 2024 Dec;6(12):2338-2353. doi

Insert

tBID-flag (BID Human)

Use

Retroviral

Tags

FLAG

Expression

Mammalian

Mutation

Truncated (CASP8) form (p15)

Available Since

Jan. 31, 2024

Availability

Academic Institutions and Nonprofits only

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pJAM1.18_PB-SDC4-PGK-Blast

Plasmid

#205563

Purpose

Express human SDC4

Depositor

Michael Elowitz

Article

Horns et al Cell. 2023 Jul 10:S0092-8674(23)00689-X.

Insert

SDC4 (SDC4 Human)

Use

Piggybac

Expression

Mammalian

Mutation

WT

Promoter

EF1

Available Since

Sept. 20, 2023

Availability

Academic Institutions and Nonprofits only

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U6-sgH11-eCas9-T2A-BlastR

Plasmid

#172492

Purpose

For CRISPR-assisted HDR, Hipp11 locus

Depositor

Tyler Jacks

Article

Freed-Pastor et al Cancer Cell. 2021 Oct 11;39(10):1342-136

Insert

, BlastR and sgRNA targeting the Hipp11 (H11) safe harbor locus

Use

CRISPR, Lentiviral, and Mouse Targeting

Available Since

June 30, 2023

Availability

Academic Institutions and Nonprofits only

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pCRISPaint-mScarlet-BlastR [M1G]

Plasmid

#171814

Purpose

Universal donor plasmid for CRISPitope method encoding mScarlet fluorescent protein and blasticidin resistance cassette [p.M1G]

Depositor

Michael Hoelzel

Article

Effern et al Immunity. 2020 Sep 15;53(3):564-580.e9.

Insert

mScarlet-T2A-BlastR

Use

Gene tagging

Mutation

BlastR: Changed Methionin 1 to Glycine.

Available Since

July 21, 2021

Availability

Academic Institutions and Nonprofits only

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Endoplasmic reticulum-targeted optodPLD

Plasmid

#140059

Purpose

Encodes CRY2-mCherry-dPLD-P2A-CIBN-Sac1(TM) for mammalian expression

Depositor

Jeremy Baskin

Article

Tei et al J Cell Biol. 2020 Mar 2;219(3). pii: 133

Insert

Phospholipase D

Tags

CIBN, CRY2, P2A, Sac1(TM), and mCherry

Expression

Mammalian

Mutation

H170A (catalytic dead)

Available Since

March 24, 2020

Availability

Academic Institutions and Nonprofits only

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pLentiCMV-Blast mLST8-Flag

Plasmid

#124915

Purpose

For stable expression sgRNA resistant Flag tagging mLST8_

Depositor

Jin Chen

Article

Hwang et al Cancer Res. 2019 Jul 1;79(13):3178-3184.

Insert

MLST8 (MLST8 Human)

Use

Lentiviral

Tags

Flag

Expression

Mammalian

Mutation

Insert ORF was sirently mutated to be resistent t…

Available Since

May 20, 2019

Availability

Academic Institutions and Nonprofits only

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pscALPS gag-gfp Blasti

Plasmid

#115803

Purpose

Express gag-gfp fusion driven by SFFV promoter

Depositor

Jeremy Luban

Article

Yurkovetskiy et al Nat Microbiol. 2018 Dec;3(12):1354-1361.

Insert

gag-gfp

Use

Lentiviral

Promoter

SFFV

Available Since

Jan. 3, 2019

Availability

Academic Institutions and Nonprofits only

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pMXS-IRES-Blast SHMT2 CD

Plasmid

#106302

Purpose

Expresses SHMT2 K280A catalytic site mutant

Depositor

Richard Possemato

Article

Minton et al Mol Cell. 2018 Feb 15;69(4):610-621.e5.

Insert

serine hydroxymethyltransferase 2 (SHMT2 Human)

Use

Retroviral

Expression

Mammalian

Mutation

K280A catalytic site mutation, Silent mutations d…

Available Since

April 30, 2018

Availability

Academic Institutions and Nonprofits only

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SHP2(WT)@pLKO-Trex-SBP

Plasmid

#85457

Purpose

Gene expression

Depositor

Darrin Stuart

Article

Chen et al Nature. 2016 Jul 7;535(7610):148-52.

Insert

SHP2 (PTPN11 Human)

Use

Lentiviral

Tags

SBP Tag

Expression

Mammalian

Promoter

RSV

Available Since

Feb. 6, 2017

Availability

Academic Institutions and Nonprofits only

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pLV-SFFVp-CRISPRoffv2.1-IRES-BlastR

Plasmid

#207181

Purpose

Lentiviral Expression Plasmid for CRISPRoff2.1 driven by SFFV Promoter

Depositor

David Bersten

Article

Allen et al bioRxiv 2023.11.21.568191

Insert

CRISPRoffv2.1

Use

CRISPR and Lentiviral

Tags

HA and TagBFP

Expression

Mammalian

Promoter

SFFVp

Available Since

April 25, 2024

Availability

Academic Institutions and Nonprofits only

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p2T-CMV-evoCjCas9-ABE8e-BlastR

Plasmid

#194060

Purpose

Mammalian expression of evoCjCas9 ABE8e

Depositor

Gerald Schwank

Article

Schmidheini et al Nat Chem Biol. 2023 Sep 21. doi: 10.1038

Insert

evoCjCas9-ABE8e

Use

CRISPR

Expression

Mammalian

Available Since

Sept. 21, 2023

Availability

Academic Institutions and Nonprofits only

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p2T-CMV-evoCjCas9-PEmax_dRnH-BlastR

Plasmid

#194063

Purpose

Mammalian expression of evoCjCas9 PEmax_deltaRnH

Depositor

Gerald Schwank

Article

Schmidheini et al Nat Chem Biol. 2023 Sep 21. doi: 10.1038

Insert

evoCjCas9-PEmax_dRnH

Use

CRISPR

Expression

Mammalian

Available Since

Sept. 21, 2023

Availability

Academic Institutions and Nonprofits only

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pCW57.1 blast OFF 3xflag IREB2

Plasmid

#169889

Purpose

Express 3x flag IREB2, suppressible by doxycycline addition

Depositor

Richard Possemato

Article

Terzi et al Sci Adv. 2021 May 26;7(22). pii: 7/22/ea

Insert

IREB2 (IREB2 Human)

Use

Lentiviral; Doxycycline mediated suppression

Tags

3x Flag

Expression

Mammalian

Mutation

Silent mutations in sgIREB2_1 and sgIREB2_2 targe…

Promoter

TRE

Available Since

May 28, 2021

Availability

Academic Institutions and Nonprofits only

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Dual opposing T7 promoters reporter plasmid

Plasmid

#156457

Purpose

A mutational reporter plasmid that can gain Kanamycin resistance upon mutation. Contains two T7 promoters flanking the kanamycin resistance gene.

Depositor

Matthew D. Shoulders

Article

Moore et al J Am Chem Soc. 2018 Sep 19;140(37):11560

Insert

NeoR/KanR

Expression

Bacterial

Mutation

ATG start codon mutated to ACG

Promoter

Em7

Available Since

Oct. 21, 2020

Availability

Academic Institutions and Nonprofits only

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pGHUC w-ERBIN (2-hybrid prey plasmid)

Plasmid

#128163

Purpose

UV5 promoter drives expression of omega-erbin fusion. Pair with pB1H2 UV5 Zif268-cons as a positive control (turns on HIS3/GFP).

Depositor

Marcus Noyes

Article

Ichikawa et al ACS Synth Biol. 2019 May 17;8(5):918-928

Insert

ERBIN PDZ domain (ERBIN Synthetic)

Use

Synthetic Biology

Tags

FLAG and Omega subunit of RNA polymerase

Expression

Bacterial

Promoter

UV5

Available Since

Jan. 3, 2020

Availability

Academic Institutions and Nonprofits only

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