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hSyn-GW-dCas9-SET2-R195G-C201A-IRES2-mCherry

Plasmid

#202090

Purpose

Neuron-specific expression vector of catalytically-dead dCas9-Set2 containing R195G and C201A mutations

Depositor

Elizabeth Heller

Article

Xu et al Neuron. 2021 Sep 15;109(18):2943-2966.e8

Insert

dCas9-Set2(R195G, C201A)

Use

Tags

3x FLAG and mCherry

Expression

Mammalian

Mutation

R195G and C201A abolish catalytic activity

Promoter

human Synapsin

Available since

Jan. 17, 2024

Availability

Academic Institutions and Nonprofits only

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pFSynW SYT9 D197, 199N IRES GFP

Plasmid

#195701

Purpose

Lentiviral plasmid encoding SYT9 with D197, 199N mutations followed by an internal ribosomal entry site followed by EGFP under the human synapsin promoter

Depositor

Edwin Chapman

Article

Seibert et al J Neurosci. 2023 Mar 1;43(9):1475-1491.

Insert

Syt9 (Syt5 Mouse)

Use

Lentiviral

Tags

Expression

Mutation

full-length mouse SYT9 with D197, 199N mutations

Promoter

Available since

Oct. 16, 2023

Availability

Academic Institutions and Nonprofits only

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pFSynW SYT9 D330, 332N IRES GFP

Plasmid

#195702

Purpose

Lentiviral plasmid encoding SYT9 with D330, 332N mutations followed by an internal ribosomal entry site followed by EGFP under the human synapsin promoter

Depositor

Edwin Chapman

Article

Seibert et al J Neurosci. 2023 Mar 1;43(9):1475-1491.

Insert

Syt9 (Syt5 Mouse)

Use

Lentiviral

Tags

Expression

Mutation

full-length mouse SYT9 with D330, 332N mutations

Promoter

Available since

Oct. 16, 2023

Availability

Academic Institutions and Nonprofits only

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AAV-RO1.7-Hsp90ab1-FLAG

Plasmid

#206355

Purpose

AAV plasmid expressing FLAG-tagged HSP90AB1 in photoreceptors

Depositor

Connie Cepko

Article

Xue et al Elife. 2024 May 10;12:RP90749. doi: 10.7

Insert

Hsp90ab1 (Hsp90ab1 Mouse)

Use

AAV

Tags

3xFLAG

Expression

Mutation

Promoter

1.7kb human red opsin

Available since

Aug. 28, 2023

Availability

Academic Institutions and Nonprofits only

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PHR-GFP-STAT2

Plasmid

#183931

Purpose

Optogenetic PHR domain coupled to GFP and STAT2 activation domain; binds to CIBN upon blue light exposure

Depositor

Karsten Rippe

Article

Trojanowski et al Mol Cell. 2022 May 4. pii: S1097-2765(22

Insert

PHR-GFP-STAT2 (CRY2 Mustard Weed, Synthetic)

Use

Tags

Expression

Mammalian

Mutation

none

Promoter

CMV

Available since

July 5, 2022

Availability

Academic Institutions and Nonprofits only

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PHR-GFP-Rta

Plasmid

#183930

Purpose

Optogenetic PHR domain coupled to GFP and Rta activation domain; binds to CIBN upon blue light exposure

Depositor

Karsten Rippe

Article

Trojanowski et al Mol Cell. 2022 May 4. pii: S1097-2765(22

Insert

PHR-GFP-Rta (CRY2 Mustard Weed, Synthetic)

Use

Tags

Expression

Mammalian

Mutation

none

Promoter

CMV

Available since

July 5, 2022

Availability

Academic Institutions and Nonprofits only

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pAAV-hSyn-mCherry-SynaptoZip

Plasmid

#177318

Purpose

AAV expression of the synaptic activity-marker SynaptoZip fused to mCherry driven by human Synapsin promoter

Depositor

Antonio Malgaroli

Article

Lamanna et al Sci Rep. 2022 Jun 30;12(1):11055. doi: 1

Insert

mCherry-SynaptoZip (Vamp2 Rat)

Use

AAV

Tags

Myc and mCherry

Expression

Mammalian

Mutation

Promoter

hSyn

Available since

June 24, 2022

Availability

Academic Institutions and Nonprofits only

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PHR-GFP-VPR

Plasmid

#183928

Purpose

Optogenetic PHR domain coupled to GFP and VPR activation domain; binds to CIBN upon blue light exposure

Depositor

Karsten Rippe

Article

Trojanowski et al Mol Cell. 2022 May 4. pii: S1097-2765(22

Insert

PHR-GFP-VPR (CRY2 Mustard Weed, Synthetic)

Use

Tags

Expression

Mammalian

Mutation

none

Promoter

CMV

Available since

June 14, 2022

Availability

Academic Institutions and Nonprofits only

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PHR-GFP-FUSN

Plasmid

#183932

Purpose

Optogenetic PHR domain coupled to GFP and FUSN domain with a high phase separation propensity; binds to CIBN upon blue light exposure

Depositor

Karsten Rippe

Article

Trojanowski et al Mol Cell. 2022 May 4. pii: S1097-2765(22

Insert

PHR-GFP-FUSN (CRY2 Mustard Weed, Synthetic)

Use

Tags

Expression

Mammalian

Mutation

none

Promoter

CMV

Available since

June 13, 2022

Availability

Academic Institutions and Nonprofits only

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pAAV.U6gT28.4.hSyn.flex.H2B.RFP

Plasmid

#170386

Purpose

Plasmid used for Crispr/cas9 based disruption of mouse Trim28, guide targeting exon 4. Cre-inducible expression of nuclear RFP.

Depositor

Johan Jakobsson

Article

Jonsson et al EMBO J. 2021 Mar 1:e106423. doi: 10.1525

Insert

Cre inducible H2B RFP

Use

AAV

Tags

Expression

Mutation

Promoter

human Synapsin

Available since

Jan. 4, 2022

Availability

Academic Institutions and Nonprofits only

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pAAV.U6gT28.3.hSyn.flex.H2B.RFP

Plasmid

#170374

Purpose

Plasmid used for Crispr/cas9 based disruption of mouse Trim28, guide targeting exon 3. Cre-inducible expression of nuclear RFP.

Depositor

Johan Jakobsson

Article

Jonsson et al EMBO J. 2021 Mar 1:e106423. doi: 10.1525

Insert

Cre inducible H2B RFP

Use

AAV

Tags

Expression

Mutation

Promoter

human Synapsin

Available since

Jan. 4, 2022

Availability

Academic Institutions and Nonprofits only

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pAAV.U6gT28.13.hSyn.flex.H2B.RFP

Plasmid

#170376

Purpose

Plasmid used for Crispr/cas9 based disruption of mouse Trim28, guide targeting exon 13. Cre-inducible expression of nuclear RFP.

Depositor

Johan Jakobsson

Article

Jonsson et al EMBO J. 2021 Mar 1:e106423. doi: 10.1525

Insert

Cre inducible H2B RFP

Use

AAV

Tags

Expression

Mutation

Promoter

human Synapsin

Available since

Jan. 4, 2022

Availability

Academic Institutions and Nonprofits only

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pEntr1a-SspB-HaloTag-TIAM1-DHPH

Plasmid

#176134

Purpose

Heterodimerization with iLID, Rac1 activation

Depositor

Dorus Gadella

Article

Mahlandt et al Elife. 2023 Jul 14;12:RP84364. doi: 10.7

Insert

TIAM1 (TIAM1 Human)

Use

Entry vector

Tags

Expression

Mutation

Promoter

Available since

Oct. 25, 2021

Availability

Academic Institutions and Nonprofits only

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LIC-Z-delCry2

Plasmid

#153545

Purpose

this is the non-clustering version of LIC-Z tagged with mcherry

Depositor

Katharina Gaus

Article

Ma et al Int J Mol Sci. 2020 May 15;21(10). pii:

Insert

human TCR ζ-Chain (CD247 Human)

Use

Tags

mCherry

Expression

Mammalian

Mutation

with Cry2 sequence deleted from LIC-Z

Promoter

CMV

Available since

July 17, 2020

Availability

Academic Institutions and Nonprofits only

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CRY-GalVP16 (B695)

Plasmid

#92031

Purpose

Expresses CRY2 (full length, intact NLS) fusion with Gal4DBD(aa1-147) fused to VP16AD (truncated), downstream of mCherry-IRES.

Depositor

Chandra Tucker

Article

Pathak et al Nucleic Acids Res. 2017 Apr 20. doi: 10.

Insert

CRY2 (CRY2 Mustard Weed)

Use

Tags

GalBD-VP16AD

Expression

Mammalian

Mutation

Promoter

Available since

July 25, 2017

Availability

Academic Institutions and Nonprofits only

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pFSW Doc2beta 6X mutant

Plasmid

#128816

Purpose

To make virus expressing Doc2beta 6X mutant

Depositor

Edwin Chapman

Article

Courtney et al Neuron. 2018 Jun 6;98(5):977-991.e5. doi

Insert

Doc2beta (Doc2b Rat)

Use

Lentiviral

Tags

IRES-EGFP

Expression

Mutation

D163A, D218A, D220N, D303A, D357A, D359A

Promoter

hSyn (human synapsin I promoter)

Available since

Availability

Academic Institutions and Nonprofits only

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pAAV-hSyn-miniDi-P2A-mCherry

Plasmid

#204358

Purpose

AAV vector for miniDi expression under the control of human synapsin promoter

Depositor

Masao Doi

Article

Miyake et al Cell Rep Methods. 2024 Oct 21;4(10):1008

Insert

miniDi-P2A-mCherry

Use

AAV

Tags

mCherry

Expression

Mutation

The third intracellular loop (ICL3) of hM4Di was …

Promoter

hSyn

Available since

Oct. 22, 2024

Availability

Academic Institutions and Nonprofits only

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pAAV-hSyn-ChRmine-mScarlet-Kv2.1-WPRE

Plasmid

#130995

Purpose

AAV vector to drive the expression of soma-targeted ChRmine-mScarlet under the control of human synapsin promoter

Depositor

Karl Deisseroth

Article

Marshel et al Science. 2019 Aug 9;365(6453). pii: scie

Has Service

AAV8

Insert

ChRmine

Use

AAV

Tags

mScarlet-Kv2.1

Expression

Mutation

Promoter

hSyn

Available since

Sept. 6, 2019

Availability

Academic Institutions and Nonprofits only

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pHR-FUSN-mCh-Cry2WT

Plasmid

#101223

Purpose

FUS(1-214) fused to mCh-Cry2WT

Depositor

Clifford Brangwynne

Article

Shin et al Cell. 2017 Jan 12;168(1-2):159-171.e14.

Insert

FUS(aa1-214) (FUS Human)

Use

Lentiviral

Tags

mCherry-Cry2

Expression

Mammalian

Mutation

Promoter

SFFV

Available since

Feb. 1, 2018

Availability

Academic Institutions and Nonprofits only

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pAAV-hSyn-miniDq-P2A-mCherry

Plasmid

#204357

Purpose

AAV vector for miniDq expression under the control of human synapsin promoter

Depositor

Masao Doi

Article

Miyake et al Cell Rep Methods. 2024 Oct 21;4(10):1008

Insert

miniDq-P2A-mCherry

Use

AAV

Tags

mCherry

Expression

Mutation

The third intracellular loop (ICL3) of hM3Dq was …

Promoter

hSyn

Available since

Oct. 22, 2024

Availability

Academic Institutions and Nonprofits only

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