We narrowed to 30,834 results for: Eng

1. AAV:ITR-U6-sgRNA(LacZ)-pCBh-Cre-WPRE-hGHpA-ITR

   Plasmid

   #60228

   Purpose

   Expresses Cre recombinase from the Cbh promoter and one U6-driven sgRNA control targeting LacZ. AAV backbone.

   Depositor

   Feng Zhang

   Article

   Platt et al Cell. 2014 Sep 24. pii: S0092-8674(14)01

   Inserts

   sgRNA
   Cre recombinase
   

   Use

   AAV, CRISPR, Cre/Lox, and Mouse Targeting

   Tags

   Cre-HA

   Expression

   Mammalian

   Mutation

   Promoter

   CBh and U6

   Available since

   Nov. 3, 2014

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pCBh_3x Flag-NLS-denOsCas12f1-NLS-VPR-NLS-mCherry_pA_phU6_gRNA scaffold-spacer_pCMV_BFP_pA

   Plasmid

   #197031

   Purpose

   Vector encoding a human codon-optimized dead enOsCas12f1 (denOsCas12f1)-VPR driven by CBh promoter, guide RNAs compatible with enOsCas12f1 driven by hU6, and BFP driven by CMV promoter

   Depositor

   Xiangfeng Kong

   Article

   Kong et al Nat Commun. 2023 Apr 11;14(1):2046. doi:

   Insert

   humanized denOsCas12f1-VPR
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   D52R / T132R / D228A / D406A

   Promoter

   CBh, CMV, hU6

   Available since

   May 8, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pRSFDuet-FBXL5-SKP1

   Plasmid

   #226620

   Purpose

   Co-expression of FBXL5-SKP1 complex in E.coli

   Depositor

   Ning Zheng

   Article

   Wang et al Mol Cell. 2020 Apr 2;78(1):31-41.e5. doi

   Inserts

   FBXL5 (FBXL5 Human)
   SKP1 (SKP1 Human)
   

   Use

   Tags

   GB1

   Expression

   Bacterial

   Mutation

   Amino acids 1-198 was deleted, and 420-596 was re…

   Promoter

   T7

   Available since

   Oct. 29, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. AAV2 ITR_phU6_gRNA scaffold-DMD sg1_phU6_gRNA scaffold-DMD sg16_pMHCK7-3x Flag-NLS-enOsCas12f1-NLS_pA_AAV2 ITR

   Plasmid

   #197028

   Purpose

   AAV vector encoding a human codon-optimized enOsCas12f1 driven by MHCK7 promoter, DMD-targeting guide RNAs compatible with enOsCas12f1 driven by hU6

   Depositor

   Xiangfeng Kong

   Article

   Kong et al Nat Commun. 2023 Apr 11;14(1):2046. doi:

   Insert

   humanized enOsCas12f1
   

   Use

   AAV

   Tags

   Expression

   Mutation

   D52R / T132R

   Promoter

   MHCK7, hU6

   Available since

   May 8, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. AAV:ITR-U6-sgRNA(NeuN)-pCBh-Cre-WPRE-hGHpA-ITR

   Plasmid

   #60227

   Purpose

   Expresses Cre recombinase from the Cbh promoter and one U6-driven sgRNA targeting the mouse gene NeuN. AAV backbone.

   Depositor

   Feng Zhang

   Article

   Platt et al Cell. 2014 Sep 24. pii: S0092-8674(14)01

   Inserts

   sgRNA
   Cre recombinase
   

   Use

   AAV, CRISPR, Cre/Lox, and Mouse Targeting

   Tags

   Cre-HA

   Expression

   Mammalian

   Mutation

   Promoter

   CBh and U6

   Available since

   Nov. 3, 2014

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pSH-Csy4-T2A-SpRFN-P2A-GFP-multi-gRNA

   Plasmid

   #85756

   Purpose

   All in one plasmid that expresses Csy4, S.pyogenes FokI-dCas9-NLS, and GFP. Also encodes for multiplexed gRNAs. Backbone derived from pSQT1601 (Addgene #53369).

   Depositor

   Lawrence Stanton

   Article

   Havlicek et al Mol Ther. 2017 Feb 1;25(2):342-355. doi:

   Inserts

   Csy4-T2A-SpRFN-P2A-GFP
   gRNA
   

   Use

   CRISPR

   Tags

   Csy4, Csy4 recognition sequence, FokI fusion via …

   Expression

   Mammalian

   Mutation

   D10A, H840A

   Promoter

   CMV and U6

   Available since

   Jan. 20, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. AAV:ITR-U6-sgRNA(LacZ)-pEFS-Rluc-2A-Cre-WPRE-hGHpA-ITR

   Plasmid

   #60225

   Purpose

   Expresses Cre recombinase and Renilla luciferase from the EFS promoter and one U6-driven sgRNA targeting LacZ. AAV backbone.

   Depositor

   Feng Zhang

   Article

   Platt et al Cell. 2014 Sep 24. pii: S0092-8674(14)01

   Inserts

   sgRNA
   Renilla luciferase
   Cre recombinase
   

   Use

   AAV, CRISPR, Cre/Lox, Luciferase, and Mouse Targe…

   Tags

   Cre-HA and Rluc-P2A

   Expression

   Mammalian

   Mutation

   Promoter

   EFS and U6

   Available since

   Oct. 16, 2014

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pML340_RAB11A-N-term_XTEN80-mEGFP-3xHA_pTMPLT

   Plasmid

   #227907

   Purpose

   Homology repair template for genome engineering. Organelle endogenous marker expressing GFP and epitope tags for mass spectrometry.

   Depositor

   Manuel Leonetti

   Article

   Hein et al Cell. 2024 Dec 26:S0092-8674(24)01344-8.

   Insert

   RAB11A homology arms with XTEN80-mEGFP-3xHA for insertion at N terminus
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Jan. 16, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. AAV2 ITR_phU6_gRNA scaffold-DMD sg1_phU6_gRNA scaffold-DMD sg16_pMHCK7-3x Flag-NLS-ecDHFR-enOsCas12f1-NLS-ecDHFR_pA_AAV2 ITR

   Plasmid

   #197029

   Purpose

   AAV vector encoding a human codon-optimized ecDHFR-enOsCas12f1-ecDHFR driven by MHCK7 promoter, DMD-targeting guide RNAs compatible with enOsCas12f1 driven by hU6

   Depositor

   Xiangfeng Kong

   Article

   Kong et al Nat Commun. 2023 Apr 11;14(1):2046. doi:

   Insert

   humanized ecDHFR-enOsCas12f1-ecDHFR
   

   Use

   AAV

   Tags

   Expression

   Mutation

   D52R / T132R

   Promoter

   MHCK7, hU6

   Available since

   May 8, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. AAV:ITR-U6-sgRNA(NeuN)-hSyn-Cre-2A-EGFP-KASH-WPRE-shortPA-ITR

    Plasmid

    #60230

    Purpose

    Expresses Cre recombinase and KASH-tagged EGFP from the hSyn promoter and one U6-driven sgRNA. AAV backbone with SapI spacer for sgRNA cloning.

    Depositor

    Feng Zhang

    Article

    Platt et al Cell. 2014 Sep 24. pii: S0092-8674(14)01

    Inserts

    sgRNA
    Cre recombinase
    EGFP
    

    Use

    AAV, CRISPR, Cre/Lox, and Mouse Targeting

    Tags

    Cre-HA-P2A and EGFP-KASH

    Expression

    Mammalian

    Mutation

    Promoter

    U6 and hSyn

    Available since

    Nov. 3, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pKan cpRBP-FL P53

    Plasmid

    #174504

    Purpose

    Express Full-length P53 in E.coli with cleavable expression/purification tag (ribose binding protein circular permutant)

    Depositor

    Stewart Loh

    Article

    Ha et al Bio Protoc. 2021 Oct 20;11(20):e4188. do

    Insert

    P53 gene (TP53 Human)
    

    Use

    Tags

    20 amino acid linker containing HRV3C protease si…

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Oct. 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pVRC8400-SARS-CoV-2-S2P-B.1.617.2-AVI

    Plasmid

    #176334

    Purpose

    Expresses SARS-CoV-2 S2P protein from the B.1.617.2 variant with single chain Fc, HRV3C protease cleavage site, and AVI tag

    Depositor

    Peter Kwong

    Article

    Teng et al PLoS One. 2022 May 24;17(5):e0268767. do

    Insert

    SARS-CoV-2-S2P-B.1.617.2 (S )
    

    Use

    Tags

    AVI, HRV3C, and scFc

    Expression

    Mammalian

    Mutation

    T19R, G142D, del156-157, R158G, L452R, T478K, D61…

    Promoter

    Available since

    March 2, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pLIB_3xFlag-His6-nsp13 (SARS-CoV-2)

    Plasmid

    #169189

    Purpose

    Baculoviral transfer vector to express 3xFlag-His6-nsp13 (SARS-CoV-2) in insect cells

    Depositor

    John Diffley

    Article

    Zeng et al Biochem J. 2021 Jul 16;478(13):2405-2423

    Insert

    3xFlag-His6-nsp13 (ORF1ab SARS-CoV-2, Synthetic)
    

    Use

    Tags

    3xFlag-His6

    Expression

    Insect

    Mutation

    Codon optimised for insect cells (Spodoptera frug…

    Promoter

    Polyhedrin

    Available since

    July 6, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pVRC8400-SARS-CoV-2-RBD-BA.2-AVI

    Plasmid

    #184533

    Purpose

    Expresses SARS-CoV-2 RBD domain from the BA.2 variant with single chain Fc, HRV3C protease cleavage site, and AVI tag

    Depositor

    Peter Kwong

    Article

    Teng et al PLoS One. 2022 May 24;17(5):e0268767. do

    Insert

    SARS-CoV-2-RBD-BA.2 (S )
    

    Use

    Tags

    AVI, HRV3C, and scFc

    Expression

    Mammalian

    Mutation

    G339D, S371F, S373P, S375F, T376A, D405N, R408S, …

    Promoter

    Available since

    May 10, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pVRC8400-SARS-CoV-2-RBD-E484K-AVI

    Plasmid

    #176444

    Purpose

    Expresses SARS-CoV-2 RBD domain containing E484K mutation with single chain Fc, HRV3C protease cleavage site, and AVI tag

    Depositor

    Peter Kwong

    Article

    Teng et al PLoS One. 2022 May 24;17(5):e0268767. do

    Insert

    SARS-CoV-2-RBD-E484K (S )
    

    Use

    Tags

    AVI, HRV3C, and scFc

    Expression

    Mammalian

    Mutation

    E484K

    Promoter

    Available since

    March 2, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pQhsCDtRNA+

    Plasmid

    #86014

    Purpose

    Multi-gene expression system for purification of human box C/D RNPs with U14-tRNA minigene

    Depositor

    Hong Li

    Article

    Peng et al PLoS One. 2014 Jul 31;9(7):e103096. doi:

    Inserts

    U14-tRNA chimera (SNORD14A Human)
    15.5K (SNU13 Human)
    fibrillarin (FBL Human)
    NOP56 (NOP56 Human)
    NOP58 (NOP58 Human)
    

    Use

    Co-expression; purification

    Tags

    6x-His

    Expression

    Bacterial

    Mutation

    amino acids 1-401, amino acids 1-411, and amino a…

    Promoter

    LPP and Ptac

    Available since

    Jan. 9, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pVRC8400-SARS-CoV-2-RBD-SD1-L452R-T478K-AVI

    Plasmid

    #176456

    Purpose

    Expresses SARS-CoV-2 RBD-SD1 domain containing L452R-T478K mutations with single chain Fc, HRV3C protease cleavage site, and AVI tag

    Depositor

    Peter Kwong

    Article

    Teng et al PLoS One. 2022 May 24;17(5):e0268767. do

    Insert

    SARS-CoV-2-RBD-SD1-L452R-T478K (S )
    

    Use

    Tags

    AVI, HRV3C, and scFc

    Expression

    Mammalian

    Mutation

    L452R, T478K

    Promoter

    Available since

    March 2, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pVRC8400-SARS-CoV-2-S2P-B.1.1.7-AVI

    Plasmid

    #176325

    Purpose

    Expresses SARS-CoV-2 S2P protein from the B.1.1.7 variant with single chain Fc, HRV3C protease cleavage site, and AVI tag

    Depositor

    Peter Kwong

    Article

    Teng et al PLoS One. 2022 May 24;17(5):e0268767. do

    Insert

    SARS-CoV-2-S2P-B.1.1.7 (S )
    

    Use

    Tags

    AVI, HRV3C, and scFc

    Expression

    Mammalian

    Mutation

    69-70 del, 144del, N501Y, A570D, D614G, P681H, T7…

    Promoter

    Available since

    March 2, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. CCR5-SZ190b-sfGFP

    Plasmid

    #162447

    Purpose

    Expression in HEK293T cell and compete ligand singaling against full-length receptors, the truncated receptor can perform signaling at high ligand concentration

    Depositor

    Shuguang Zhang

    Article

    Qing et al iScience. 2020 Oct 28;23(12):101670. doi

    Insert

    C-C chemokine receptor type 5 (CCR5 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    truncation from aa88 to aa249

    Promoter

    CMV

    Available since

    Aug. 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pVRC8400-SARS-CoV-2-NTD-BA.2-AVI

    Plasmid

    #184532

    Purpose

    Expresses SARS-CoV-2 NTD domain from the BA.2 variant with single chain Fc, HRV3C protease cleavage site, and AVI tag

    Depositor

    Peter Kwong

    Article

    Teng et al PLoS One. 2022 May 24;17(5):e0268767. do

    Insert

    SARS-CoV-2-NTD-BA.2 (S )
    

    Use

    Tags

    AVI, HRV3C, and scFc

    Expression

    Mammalian

    Mutation

    T19I, L24-, P25-, P26-, A27S, G142D, V213G

    Promoter

    Available since

    May 10, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pVRC8400-SARS-CoV-2-S2P-B.1.351-AVI

    Plasmid

    #176326

    Purpose

    Expresses SARS-CoV-2 S2P protein from the B.1.351 variant with single chain Fc, HRV3C protease cleavage site, and AVI tag

    Depositor

    Peter Kwong

    Article

    Teng et al PLoS One. 2022 May 24;17(5):e0268767. do

    Insert

    SARS-CoV-2-S2P-B.1.351 (S )
    

    Use

    Tags

    AVI, HRV3C, and scFc

    Expression

    Mammalian

    Mutation

    L18F, D80A, D215G, 242-244del, R246I, K417N, E484…

    Promoter

    Available since

    March 2, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pVRC8400-SARS-CoV-2-NTD-P.1-AVI

    Plasmid

    #176429

    Purpose

    Expresses SARS-CoV-2 NTD domain from the P.1 variant with single chain Fc, HRV3C protease cleavage site, and AVI tag

    Depositor

    Peter Kwong

    Article

    Teng et al PLoS One. 2022 May 24;17(5):e0268767. do

    Insert

    SARS-CoV-2-NTD-P.1 (S )
    

    Use

    Tags

    AVI, HRV3C, and scFc

    Expression

    Mammalian

    Mutation

    L18F, T20N, P26S, D138Y, R190S

    Promoter

    Available since

    March 2, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pVRC8400-SARS-CoV-2-S2P-P.1-AVI

    Plasmid

    #176327

    Purpose

    Expresses SARS-CoV-2 S2P protein from the P.1 variant with single chain Fc, HRV3C protease cleavage site, and AVI tag

    Depositor

    Peter Kwong

    Article

    Teng et al PLoS One. 2022 May 24;17(5):e0268767. do

    Insert

    SARS-CoV-2-S2P-P.1 (S )
    

    Use

    Tags

    AVI, HRV3C, and scFc

    Expression

    Mammalian

    Mutation

    L18F, T20N, P26S, D138Y, R190S, K417T, E484K, N50…

    Promoter

    Available since

    March 2, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pZE21/UBP1/ClpS_V65I

    Plasmid

    #98567

    Purpose

    Expresses truncated codon-opt S. cerevisiae UBP1 and the V65I engineered variant of E. coli ClpS in an artificial operon

    Depositor

    George Church

    Article

    Kunjapur et al Proc Natl Acad Sci U S A. 2018 Jan 16;11

    Inserts

    Truncated ubiquitin cleavase, codon optimized
    Mutated ATP-dependent Clp protease adapter protein
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Contains the V65I mutation that increases discrim…

    Promoter

    Available since

    Feb. 7, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. Icam 2 SaCas9 YAP sgRNA3

    Plasmid

    #99736

    Purpose

    Expresses SaCas9 and a gRNA targeting mouse YAP

    Depositor

    Yu Huang

    Article

    Wang et al Nature. 2016 Dec 7. doi: 10.1038/nature2

    Insert

    SaCas9 gRNA targeting Yap1 (Yap1 Mouse)
    

    Use

    AAV and CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Dec. 11, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pAAV-TNT4-Zfr-P2A-HA-luciferase-3xMIR122

    Plasmid

    #223184

    Purpose

    Plasmid containing the Zfr/Firefly luciferase/3xMIR122 cassette. Translation of luciferase is controlled by splicing modulation of the Zfr specifically in cardiomyocytes by risdiplam in vivo.

    Depositor

    Yuxuan Guo

    Article

    Chen et al Nat Cardiovasc Res. 2025 Jun 13. doi: 10

    Inserts

    luciferase (LOC116160065 firefly)
    mir122 (Mir122 Mouse)
    

    Use

    AAV

    Tags

    HA and engineered P2A

    Expression

    Mutation

    Promoter

    TNNT2

    Available since

    June 18, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pAAV-TNT4-Zfr-P2A-HA-YAP5SA

    Plasmid

    #223189

    Purpose

    Plasmid containing the Zfr/YAP5SA cassette. Translation of YAP5SA is controlled by splicing modulation of the Zfr by risdiplam.

    Depositor

    Yuxuan Guo

    Article

    Chen et al Nat Cardiovasc Res. 2025 Jun 13. doi: 10

    Insert

    YAP5SA (Yap1 Mouse)
    

    Use

    AAV

    Tags

    HA and engineered P2A

    Expression

    Mutation

    S61A, S109A, S127A, S164A, S381A

    Promoter

    TNNT2

    Available since

    June 18, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pML625_RTN4-N-term_XTEN80-mEGFP-3xHA_pTMPLT

    Plasmid

    #227895

    Purpose

    Homology repair template for genome engineering. Organelle endogenous marker expressing GFP and epitope tags for mass spectrometry.

    Depositor

    Manuel Leonetti

    Article

    Hein et al Cell. 2024 Dec 26:S0092-8674(24)01344-8.

    Insert

    RTN4 homology arms with mEGFP-3xHA for insertion at N terminus
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    April 10, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pML624_RAB1A-N-term_XTEN80-mEGFP-3xHA_pTMPLT

    Plasmid

    #227906

    Purpose

    Homology repair template for genome engineering. Organelle endogenous marker expressing GFP and epitope tags for mass spectrometry.

    Depositor

    Manuel Leonetti

    Article

    Hein et al Cell. 2024 Dec 26:S0092-8674(24)01344-8.

    Insert

    RAB1A homology arms with XTEN80-mEGFP-3xHA for insertion at N terminus
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    April 10, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. YjdC-R82A_pET-14b

    Plasmid

    #233276

    Purpose

    Overexpress his-tag YjdC protein with a site directed mutagenesis, arginine at position 82 was substituted with alanine (R82A)

    Depositor

    Andrew Emili, Mohan Babu

    Article

    Peng et al Cell. 2025 Jan 22:S0092-8674(25)00032-7.

    Insert

    YjdC (yjdC )
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Arginine was replaced with Alanine at position 82…

    Promoter

    T7 promoter

    Available since

    March 17, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. YjdC-R64A_pET-14b

    Plasmid

    #233263

    Purpose

    Overexpress his-tag YjdC protein with a site directed mutagenesis, arginine at position 64 was substituted with alanine (R64A)

    Depositor

    Andrew Emili, Mohan Babu

    Article

    Peng et al Cell. 2025 Jan 22:S0092-8674(25)00032-7.

    Insert

    YjdC (yjdC )
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Arginine was replaced with Alanine at position 64…

    Promoter

    T7 promoter

    Available since

    March 17, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. HemH-R212A_pET-14b

    Plasmid

    #233262

    Purpose

    Overexpress his-tag HemH protein with a site directed mutagenesis, arginine at position 212 was substituted with alanine (R121A)

    Depositor

    Andrew Emili, Mohan Babu

    Article

    Peng et al Cell. 2025 Jan 22:S0092-8674(25)00032-7.

    Insert

    HemH (hemH )
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Arginine was replaced with Alanine at position 21…

    Promoter

    T7 promoter

    Available since

    March 11, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pML484_RPL36-C-term_XTEN80-mEGFP-3xHA_pTMPLT

    Plasmid

    #227923

    Purpose

    Homology repair template for genome engineering. Organelle endogenous marker expressing GFP and epitope tags for mass spectrometry.

    Depositor

    Manuel Leonetti

    Article

    Hein et al Cell. 2024 Dec 26:S0092-8674(24)01344-8.

    Insert

    RPL36 homology arms with XTEN80-mEGFP-3xHA for insertion at C terminus
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 16, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pML390_YWHAQ-C-term_XTEN80-mEGFP-3xHA_pTMPLT

    Plasmid

    #227925

    Purpose

    Homology repair template for genome engineering. Organelle endogenous marker expressing GFP and epitope tags for mass spectrometry.

    Depositor

    Manuel Leonetti

    Article

    Hein et al Cell. 2024 Dec 26:S0092-8674(24)01344-8.

    Insert

    YWHAQ homology arms with XTEN80-mEGFP-3xHA for insertion at C terminus
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 16, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pML389_YWHAB-C-term_XTEN80-mEGFP-3xHA_pTMPLT

    Plasmid

    #227926

    Purpose

    Homology repair template for genome engineering. Organelle endogenous marker expressing GFP and epitope tags for mass spectrometry.

    Depositor

    Manuel Leonetti

    Article

    Hein et al Cell. 2024 Dec 26:S0092-8674(24)01344-8.

    Insert

    YWHAB homology arms with XTEN80-mEGFP-3xHA for insertion at C terminus
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 16, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pML343_RAB14-N-term_XTEN80-mEGFP-3xHA_pTMPLT

    Plasmid

    #227908

    Purpose

    Homology repair template for genome engineering. Organelle endogenous marker expressing GFP and epitope tags for mass spectrometry.

    Depositor

    Manuel Leonetti

    Article

    Hein et al Cell. 2024 Dec 26:S0092-8674(24)01344-8.

    Insert

    RAB14 homology arms with XTEN80-mEGFP-3xHA for insertion at N terminus
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 16, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pML325_GPR107-C-term_XTEN80-mEGFP-3xHA_pTMPLT

    Plasmid

    #227909

    Purpose

    Homology repair template for genome engineering. Organelle endogenous marker expressing GFP and epitope tags for mass spectrometry.

    Depositor

    Manuel Leonetti

    Article

    Hein et al Cell. 2024 Dec 26:S0092-8674(24)01344-8.

    Insert

    GPR107 homology arms with XTEN80-mEGFP-3xHA for insertion at C terminus
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 16, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pML323_GOLGA2-N-term_XTEN80-mEGFP-3xHA_pTMPLT

    Plasmid

    #227910

    Purpose

    Homology repair template for genome engineering. Organelle endogenous marker expressing GFP and epitope tags for mass spectrometry.

    Depositor

    Manuel Leonetti

    Article

    Hein et al Cell. 2024 Dec 26:S0092-8674(24)01344-8.

    Insert

    GOLGA2 homology arms with XTEN80-mEGFP-3xHA for insertion at N terminus
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 16, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pML600_COG8-C-term_XTEN80-mEGFP-3xHA_pTMPLT

    Plasmid

    #227911

    Purpose

    Homology repair template for genome engineering. Organelle endogenous marker expressing GFP and epitope tags for mass spectrometry.

    Depositor

    Manuel Leonetti

    Article

    Hein et al Cell. 2024 Dec 26:S0092-8674(24)01344-8.

    Insert

    COG8 homology arms with XTEN80-mEGFP-3xHA for insertion at C terminus
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 16, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pML494_ATP1B3-C-term_XTEN80-sfGFP-3xHA_pTMPLT

    Plasmid

    #227912

    Purpose

    Homology repair template for genome engineering. Organelle endogenous marker expressing GFP and epitope tags for mass spectrometry.

    Depositor

    Manuel Leonetti

    Article

    Hein et al Cell. 2024 Dec 26:S0092-8674(24)01344-8.

    Insert

    ATP1B3 homology arms with XTEN80-sfGFP-3xHA for insertion at C terminus
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 16, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

41. pML499_SLC3A2-C-term_XTEN80-sfGFP-3xHA_pTMPLT

    Plasmid

    #227913

    Purpose

    Homology repair template for genome engineering. Organelle endogenous marker expressing GFP and epitope tags for mass spectrometry.

    Depositor

    Manuel Leonetti

    Article

    Hein et al Cell. 2024 Dec 26:S0092-8674(24)01344-8.

    Insert

    SLC3A2 homology arms with XTEN80-sfGFP-3xHA for insertion at C terminus
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 16, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

42. pML357_CAV1-C-term_XTEN80-mEGFP-3xHA_pTMPLT

    Plasmid

    #227914

    Purpose

    Homology repair template for genome engineering. Organelle endogenous marker expressing GFP and epitope tags for mass spectrometry.

    Depositor

    Manuel Leonetti

    Article

    Hein et al Cell. 2024 Dec 26:S0092-8674(24)01344-8.

    Insert

    CAV1 homology arms with XTEN80-mEGFP-3xHA for insertion at C terminus
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 16, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

43. pML454_G3BP1-N-term_XTEN80-mEGFP-3xHA_pTMPLT

    Plasmid

    #227915

    Purpose

    Homology repair template for genome engineering. Organelle endogenous marker expressing GFP and epitope tags for mass spectrometry.

    Depositor

    Manuel Leonetti

    Article

    Hein et al Cell. 2024 Dec 26:S0092-8674(24)01344-8.

    Insert

    G3BP1 homology arms with XTEN80-mEGFP-3xHA for insertion at N terminus
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 16, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

44. pML359_CAPRIN1-N-term_XTEN80-mEGFP-3xHA_pTMPLT

    Plasmid

    #227916

    Purpose

    Homology repair template for genome engineering. Organelle endogenous marker expressing GFP and epitope tags for mass spectrometry.

    Depositor

    Manuel Leonetti

    Article

    Hein et al Cell. 2024 Dec 26:S0092-8674(24)01344-8.

    Insert

    CAPRIN1 homology arms with XTEN80-mEGFP-3xHA for insertion at N terminus
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 16, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

45. pML361_DCP1A-N-term_XTEN80-mEGFP-3xHA_pTMPLT

    Plasmid

    #227918

    Purpose

    Homology repair template for genome engineering. Organelle endogenous marker expressing GFP and epitope tags for mass spectrometry.

    Depositor

    Manuel Leonetti

    Article

    Hein et al Cell. 2024 Dec 26:S0092-8674(24)01344-8.

    Insert

    DCP1A homology arms with XTEN80-mEGFP-3xHA for insertion at N terminus
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 16, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

46. pML366_HSPA1B-N-term_XTEN80-mEGFP-3xHA_pTMPLT

    Plasmid

    #227920

    Purpose

    Homology repair template for genome engineering. Organelle endogenous marker expressing GFP and epitope tags for mass spectrometry.

    Depositor

    Manuel Leonetti

    Article

    Hein et al Cell. 2024 Dec 26:S0092-8674(24)01344-8.

    Insert

    HSPA1B homology arms with XTEN80-mEGFP-3xHA for insertion at N terminus
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 16, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

47. pML365_HSP90AA1-N-term_XTEN80-mEGFP-3xHA_pTMPLT

    Plasmid

    #227921

    Purpose

    Homology repair template for genome engineering. Organelle endogenous marker expressing GFP and epitope tags for mass spectrometry.

    Depositor

    Manuel Leonetti

    Article

    Hein et al Cell. 2024 Dec 26:S0092-8674(24)01344-8.

    Insert

    HSP90AA homology arms with XTEN80-mEGFP-3xHA for insertion at N terminus
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 16, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

48. pML364_PSMB7-C-term_XTEN80-mEGFP-3xHA_pTMPLT

    Plasmid

    #227922

    Purpose

    Homology repair template for genome engineering. Organelle endogenous marker expressing GFP and epitope tags for mass spectrometry.

    Depositor

    Manuel Leonetti

    Article

    Hein et al Cell. 2024 Dec 26:S0092-8674(24)01344-8.

    Insert

    PSMB7 homology arms with XTEN80-mEGFP-3xHA for insertion at C terminus
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 16, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

49. pML346_VPS35-C-term_XTEN80-mEGFP-3xHA_pTMPLT

    Plasmid

    #227891

    Purpose

    Homology repair template for genome engineering. Organelle endogenous marker expressing GFP and epitope tags for mass spectrometry.

    Depositor

    Manuel Leonetti

    Article

    Hein et al Cell. 2024 Dec 26:S0092-8674(24)01344-8.

    Insert

    VPS35 homology arms with mEGFP-3xHA for insertion at C terminus
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 16, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

50. pML530_EEA1-N-term_XTEN80-mEGFP-3xHA_pTMPLT

    Plasmid

    #227892

    Purpose

    Homology repair template for genome engineering. Organelle endogenous marker expressing GFP and epitope tags for mass spectrometry.

    Depositor

    Manuel Leonetti

    Article

    Hein et al Cell. 2024 Dec 26:S0092-8674(24)01344-8.

    Insert

    EEA1 homology arms with mEGFP-3xHA for insertion at N terminus
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 16, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge