We narrowed to 15,050 results for: pET

1. pET-16b-10His-TEV-MCES_VACCA_D1_D12

   Plasmid

   #203671

   Purpose

   The D1 and D12 subunits of the vaccinia capping enzyme were cloned in tandem into the vector pET-16b (Novagen). An N-terminal His10 tag was added to the D1 subunit and the original factor Xa cleavage

   Depositor

   Stephen Cusack

   Article

   Kyrieleis et al Structure. 2014 Mar 4;22(3):452-65. doi:

   Insert

   MCES_VACCA_D1_D12
   

   Tags

   10xHis

   Expression

   Bacterial

   Available Since

   Aug. 3, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pET22b-PelB-6H-Ag43-160N-sfGFP

   Plasmid

   #172886

   Purpose

   Display sfGFP protein on E. coli cell surface

   Depositor

   Urartu Seker

   Article

   Ahan et al ACS Synth Biol. 2019 Apr 19;8(4):686-696

   Inserts

   SfGFP
   Ag43, partial (flu )
   

   Tags

   HHHHHH and TEV recognition site (ENLYFQG)

   Expression

   Bacterial

   Mutation

   Deleted 1-160 (N terminal)

   Promoter

   T7 Promoter

   Available Since

   June 3, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. PET-B-Sniper SpCas9-NLS-6xHis

   Plasmid

   #207377

   Purpose

   Expression of increased-fidelity (i.e. high-fidelity) SpCas9 variant in bacterial cells

   Depositor

   Ervin Welker

   Article

   Kulcsar et al Nat Commun. 2023 Sep 16;14(1):5746. doi:

   Insert

   B-Sniper SpCas9
   

   Use

   CRISPR

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   F539S, M763I, K890N, amino acids 1005-1013 replac…

   Promoter

   T7

   Available Since

   Sept. 25, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. pet28a-His6-Halo-TEV-Nrf2

   Plasmid

   #62455

   Purpose

   Halo-His6 tagged Nrf2 for E.coli expression

   Depositor

   Yimon Aye

   Article

   Parvez et al J Am Chem Soc. 2014 Dec 31.

   Insert

   Nrf2 (NFE2L2 Human)
   

   Tags

   His6-Halo-TEV

   Expression

   Bacterial

   Available Since

   April 6, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. pET-26b-NbVHH05-ALFA-His

   Plasmid

   #171569

   Purpose

   Bacterial expression of ALFA-His-tagged anti-UBC6e (human) recombinant alpaca nanobody (NbVHH05-ALFA-His)

   Depositor

   Norbert Perrimon, Hidde Ploegh

   Article

   Xu et al Elife. 2022 Jan 25;11. pii: 74326. doi:

   Insert

   NbVHH05-ALFA-His (UBE2J1 Human)
   

   Tags

   ALFA-tag/His-tag and PelB signal peptide

   Expression

   Bacterial

   Available Since

   June 30, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pET-29b(+)_His-CBD-3C-EmPNGaseF

   Plasmid

   #218193

   Purpose

   Bacterial expression of a His-tagged Chitin Binding fusion of Elizabethkingia miricola PNGaseF

   Depositor

   Florence Pojer

   Insert

   PNGaseF
   

   Tags

   tufA-His-CBD-3C

   Expression

   Bacterial

   Mutation

   codon optimized Ecoli, residues 41-354

   Promoter

   T7

   Available Since

   April 30, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pET-21a(+)/MBP-His-PINK1

   Plasmid

   #38005

   Depositor

   Michael J Fox Foundation MJFF

   Insert

   PINK1 (PINK1 Human)
   

   Tags

   6xHIS and MBP

   Expression

   Bacterial

   Available Since

   March 10, 2014

   Availability

   Academic Institutions and Nonprofits only

8. PET-Sniper SpCas9-NLS-6xHis

   Plasmid

   #207374

   Purpose

   Expression of increased-fidelity (i.e. high-fidelity) SpCas9 variant in bacterial cells

   Depositor

   Ervin Welker

   Article

   Kulcsar et al Nat Commun. 2023 Sep 16;14(1):5746. doi:

   Insert

   Sniper SpCas9
   

   Use

   CRISPR

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   F539S, M763I, K890N

   Promoter

   T7

   Available Since

   Sept. 25, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. PET-28a-YTH domain of YTHDF2

   Plasmid

   #228776

   Purpose

   Protein expression plasmid for YTH domain of YTHDF2 in E.Coli.

   Depositor

   Huiqing Zhou

   Article

   Wang et al RSC Chem Biol. 2024 Jul 25;5(9):914-923.

   Insert

   YTH domain of YTHDF2 (YTHDF2 Human)
   

   Tags

   6*His

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Jan. 2, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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10. pET21-10XHis-GST-HRV-dL5

    Plasmid

    #73214

    Purpose

    Bacterial expression vector for dL5(E52D) protein with N-terminal His10, GST and HRV3C cleavage site (MBIC5, dL5**, FAP)

    Depositor

    Marcel Bruchez

    Article

    Wang et al Bioconjug Chem. 2015 Jan 21;26(1):137-44

    Insert

    EGFR (EGFR Human, Schistosoma japonicum)
    

    Tags

    10XHis-GST-HRV

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    March 9, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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11. PET-HiFi SpCas9-NLS-6xHis

    Plasmid

    #207376

    Purpose

    Expression of increased-fidelity (i.e. high-fidelity) SpCas9 variant in bacterial cells

    Depositor

    Ervin Welker

    Article

    Kulcsar et al Nat Commun. 2023 Sep 16;14(1):5746. doi:

    Insert

    HiFi SpCas9
    

    Use

    CRISPR

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    R691A

    Promoter

    T7

    Available Since

    Sept. 25, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. pETDuet-1 Hepatitis C Virus NS3/NS4A

    Plasmid

    #61696

    Purpose

    inducible dual expression of NS3 protease along with its cofactor NS4A

    Depositor

    Michael Johnson

    Article

    Lee et al PLoS One. 2013 Oct 9;8(10):e75144. doi:

    Inserts

    NS4A
    NS3
    

    Tags

    his

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    April 10, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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13. pET51b-His-TEV-SNAP-tag_fast

    Plasmid

    #167271

    Purpose

    Overexpression of the fast variant (E30R) of SNAP-tag in E. coli

    Depositor

    Kai Johnsson

    Article

    Wilhelm et al Biochemistry. 2021 Aug 24;60(33):2560-25

    Insert

    SNAPf-tag
    

    Tags

    His x10

    Expression

    Bacterial

    Mutation

    E30R

    Promoter

    T7

    Available Since

    April 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. pET-30a-NSP14-SARS-CoV-2

    Plasmid

    #236423

    Purpose

    bacterial protein expression of NSP14

    Depositor

    Michele Pagano

    Article

    Rona et al Cell Death Differ. 2022 Feb;29(2):285-29

    Insert

    NSP14 (ORF1ab SARS-CoV-2)
    

    Tags

    6xHis

    Expression

    Bacterial

    Available Since

    May 28, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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15. pET21b(+)_SARS-CoV-2_3CLpro-Q306A

    Plasmid

    #177334

    Purpose

    bacterial expression of active SARS-CoV-2 3C-like proteinase

    Depositor

    Christopher Overall

    Article

    Pablos et al Cell Rep. 2021 Oct 26;37(4):109892. doi:

    Insert

    SARS-CoV-2 3C-like proteinase (ORF1ab Severe acute respiratory syndrome coronavirus 2) (ORF1ab SARS-CoV-2 virus)
    

    Tags

    Factor Xa site-3xFlag-Myc-6xHis

    Expression

    Bacterial

    Mutation

    silent mutation C to T at position 10546 (elimina…

    Promoter

    T7

    Available Since

    Nov. 23, 2021

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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16. pPS22 (pET23a-Cas10/Csm/csm2H6N)

    Plasmid

    #70038

    Purpose

    Bacterial expression of the S. epidermidis Cas10·Csm complex

    Depositor

    Luciano Marraffini

    Article

    Hatoum-Aslan et al J Biol Chem. 2013 Sep 27;288(39):27888-9

    Insert

    Cas10/Csm complex
    

    Tags

    6xHIS on N terminus of Csm2

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Oct. 20, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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17. pET-28a+GFP-CAMSAP3 CKK

    Plasmid

    #59033

    Purpose

    expresses CKK domain of CAMSAP3 in E. coli

    Depositor

    Ron Vale

    Article

    Hendershott et al Proc Natl Acad Sci U S A. 2014 Apr 22;11

    Insert

    CAMSAP3 CKK domain (CAMSAP3 Mouse)
    

    Tags

    His, Strep, TEV, and sfGFP

    Expression

    Bacterial

    Available Since

    Sept. 2, 2014

    Availability

    Academic Institutions and Nonprofits only

18. pET-26b-Nb127D01-ALFA-His

    Plasmid

    #171568

    Purpose

    Bacterial expression of ALFA-His-tagged anti-CXCR2 (human) recombinant llama nanobody (Nb127D01-ALFA-His)

    Depositor

    Norbert Perrimon, Hidde Ploegh

    Article

    Xu et al Elife. 2022 Jan 25;11. pii: 74326. doi:

    Insert

    Nb127D01-ALFA-His (CXCR2 Human)
    

    Tags

    ALFA-tag/His-tag and PelB signal peptide

    Expression

    Bacterial

    Available Since

    June 30, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. [pSK122] pETDuet GST-p18(delta5)

    Plasmid

    #136144

    Purpose

    bacterial expression of GST-p18

    Depositor

    Kuang Shen, David Sabatini

    Article

    Shen et al Proc Natl Acad Sci U S A. 2018 Sep 18;11

    Insert

    GST-p18
    

    Tags

    GST

    Expression

    Bacterial

    Available Since

    Feb. 27, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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20. pET-21a(+)-BMTU UDG-6xHis

    Plasmid

    #172197

    Purpose

    expresses His-tagged uracil-DNA-glycosylase enzyme from marine bacterium BMTU 3346 for the purpose of cross contamination prevention in nucleic acid amplification reactions

    Depositor

    Andrea Pauli

    Insert

    BMTU UDG
    

    Tags

    6xHis tag

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    April 3, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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21. pET-22b(+) pCopA RBS30 mscarlet

    Plasmid

    #226370

    Purpose

    Plasmid carries copper sensor circuit genes for whole-cell sensing of copper ions with RBS30

    Depositor

    Urartu Seker

    Article

    Koksaldi et al Biosens Bioelectron. 2024 Aug 10;264:116

    Insert

    mScarlet
    

    Tags

    6x Histag

    Expression

    Bacterial

    Promoter

    copA promoter (pCopA)

    Available Since

    Feb. 5, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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22. pET51b(+)-NADPH-LUCID-3 (C50 1.4 uM)

    Plasmid

    #117910

    Purpose

    Plasmid encoding the bacterial expression of the BRET semi-synthetic biosensor measuring the NADPH concentration with a C50 of 1.4 uM

    Depositor

    Kai Johnsson

    Article

    Yu et al Science. 2018 Sep 14;361(6407):1122-1126

    Insert

    SNAP_Pro30_[cpNLuc23_24]eDHFR(R44A)
    

    Tags

    His-tag and Strep-tag

    Expression

    Bacterial

    Mutation

    R44A on eDHFR

    Promoter

    T7

    Available Since

    Oct. 25, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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23. pET22b Ag43 160N 6H sfGFP-R5 AmpR

    Plasmid

    #225139

    Purpose

    Recombinant Ag43 protein fused to silaffin-R5 and sfGFP. pelB as the periplasmic signal. His-tag for purification. TEV enzyme recognition site between Ag43 alpha subunit and the fluorescent protein.

    Depositor

    Urartu Seker

    Article

    Kirpat Konak et al Mater Today Bio. 2022 Oct 10;17:100461.

    Insert

    Ag43 protein fused to silaffin-R5 and sfGFP
    

    Tags

    His tag

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Feb. 5, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. PET-28a-YTH domain of YTHDF1

    Plasmid

    #228775

    Purpose

    Protein expression plasmid for YTH domain of YTHDF1 in E.Coli.

    Depositor

    Huiqing Zhou

    Article

    Wang et al RSC Chem Biol. 2024 Jul 25;5(9):914-923.

    Insert

    YTH domain of YTHDF1 (YTHDF1 Human)
    

    Tags

    6*His

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Jan. 2, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pET28a-GGG-ELP(40nm)-Cys

    Plasmid

    #91571

    Purpose

    Elastin Like Polypeptide: [(VPGEG)-(VPGVG)4-(VPGAG)2-(VPGGG)2-(VPGEG)]2, with N-Terminal Sortase Recognition Sequence GGG and a C-terminal Cysteine

    Depositor

    Hermann Gaub

    Article

    Ott et al ACS Nano. 2017 Jun 12. doi: 10.1021/acsn

    Insert

    GGG-ELP(40nm)-Cys
    

    Tags

    Cysteine and Sortase-Tag

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Feb. 1, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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26. PET-28a-YTH domain of YTHDF3

    Plasmid

    #228777

    Purpose

    Protein expression plasmid for YTH domain of YTHDF3 in E.Coli.

    Depositor

    Huiqing Zhou

    Article

    Wang et al RSC Chem Biol. 2024 Jul 25;5(9):914-923.

    Insert

    YTH domain of YTHDF3 (YTHDF3 Human)
    

    Tags

    6*His

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Jan. 2, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pET-29b-3xGluGlu-c-jun-His6

    Plasmid

    #129227

    Purpose

    Encodes 3xGluGlu tagged human c-Jun for purification by nickel affinity

    Depositor

    Kevin Janes

    Article

    Smolko et al Sci Rep. 2019 Dec 19;9(1):19409. doi: 10

    Insert

    cJun (JUN Human)
    

    Tags

    6xHistidine

    Expression

    Bacterial

    Available Since

    Jan. 21, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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28. pET51b-His-TEV-CLIP-tag_fast

    Plasmid

    #167275

    Purpose

    Overexpression of the fast variant (E30R) of CLIP-tag in E. coli

    Depositor

    Kai Johnsson

    Article

    Wilhelm et al Biochemistry. 2021 Aug 24;60(33):2560-25

    Insert

    CLIPf-tag
    

    Tags

    His x10

    Expression

    Bacterial

    Mutation

    E30R

    Promoter

    T7

    Available Since

    April 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pETDuet-1_6xHis-TEV-mCh-NDP52

    Plasmid

    #187829

    Purpose

    Plasmid for the expression and purification of 6xHis-TEV-mCh-NDP52 in E. coli Rosetta pLysS. Internal Reference: SMC1148

    Depositor

    Sascha Martens

    Article

    Nguyen et al Mol Cell. 2023 May 18;83(10):1693-1709.e

    Insert

    NDP52 (CALCOCO2 Human)
    

    Tags

    6xHisTag, TEV, and mCherry

    Expression

    Bacterial, Insect, and Mamm…

    Available Since

    Oct. 14, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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30. pET-Duet1_6xHis-TEV-eGFP-P62

    Plasmid

    #190929

    Purpose

    Plasmid for the expression and purification of GFP labelled p62. Internal reference: SMC390

    Depositor

    Sascha Martens

    Article

    Turco et al Nat Commun. 2021 Sep 1;12(1):5212. doi:

    Insert

    P62 (SQSTM1 Human)
    

    Tags

    EGFP

    Expression

    Bacterial

    Available Since

    Sept. 29, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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