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We narrowed to 8,387 results for: BLI;

Showing: 4881 - 4920 of 8387 results

  1. pT7CFE1_EVA71_VP12A_C110A

    Plasmid
    #212976
    Purpose
    Encodes EV-A71 4643 VP12A with active site mutation optimized for expression in mammalian transcription/translation extracts
    Depositor
    Karla Kirkegaard
    Insert
    Enterovirus A71 4643 VP12A C110A
    Use
    Mammalian cell free protein expression
    Tags
    HA tag
    Expression
    Mutation
    changed cysteine 110 from 2A to alanine
    Promoter
    T7
    Available since
    Jan. 26, 2024
    Availability
    Academic Institutions and Nonprofits only
    212976-plasmid-map-sequence-id-420360

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  2. pT7CFE1_polio_VP12A_C109R

    Plasmid
    #212987
    Purpose
    Encodes Poliovirus type I Mahoney VP12A with active site mutation optimized for expression in mammalian transcription/translation extracts
    Depositor
    Karla Kirkegaard
    Insert
    Poliovirus Type I Mahoney VP12A C109R
    Use
    Mammalian cell free protein expression
    Tags
    HA
    Expression
    Mutation
    Cysteine 109 from 2A mutated to arginine
    Promoter
    T7
    Available since
    Jan. 26, 2024
    Availability
    Academic Institutions and Nonprofits only
    212987-plasmid-map-sequence-id-420359

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  3. pCHA_SBV_NSs

    Plasmid
    #208938
    Purpose
    Expresses C-terminally HA-tagged Schmallenberg orthobunyavirus NSs protein from CMV promoter in mammalian cells
    Depositor
    Gerald Barry
    Insert
    NSs
    Use
    Tags
    HA
    Expression
    Mammalian
    Mutation
    Deleted 47 bp at 5' end and 510 bp from 3&#x…
    Promoter
    CMV
    Available since
    Jan. 11, 2024
    Availability
    Academic Institutions and Nonprofits only
    208938-plasmid-map-sequence-id-419026

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  4. pET28-Flag-RBM24-95~236

    Plasmid
    #208634
    Purpose
    for E. coli expression of truncated Flag tagged human RBM24 (ENST00000379052), aa95~236
    Depositor
    Quanfu Ma
    Insert
    human RBM24 (ENST00000216146) aa 95~236 (RBM24 Human)
    Use
    Tags
    6xHis and Flag
    Expression
    Bacterial
    Mutation
    only aa 95~236, other residues not included
    Promoter
    T7
    Available since
    Jan. 9, 2024
    Availability
    Academic Institutions and Nonprofits only
    208634-plasmid-map-sequence-id-418349

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  5. pAS_MmaPylT_EF1_NES-MmaPylRS WT

    Plasmid
    #174901
    Purpose
    expression of Mma PylRS with nuclear export sequence for amber suppression in mammalian cells; transient or piggy bac mediated integration
    Depositor
    Simon Elsaesser
    Insert
    Mma PylRS (pylS Methanosarcina mazei)
    Use
    Tags
    FLAG and NES
    Expression
    Mammalian
    Mutation
    Promoter
    EF1
    Available since
    Nov. 8, 2023
    Availability
    Academic Institutions and Nonprofits only
    174901-plasmid-map-sequence-id-348003

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  6. pAGW-Fray

    Plasmid
    #203979
    Purpose
    GFP-Fray under actin promoter for cell culture
    Depositor
    Andreas Jenny
    Insert
    Frayed (fray Fly)
    Use
    Tags
    EGFP
    Expression
    Insect
    Mutation
    Promoter
    Available since
    Oct. 4, 2023
    Availability
    Academic Institutions and Nonprofits only
    203979-plasmid-map-sequence-id-403220

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  7. pAMW-WnkAAxV

    Plasmid
    #203980
    Purpose
    Myc6 Wnk under actin promoter for cell culture
    Depositor
    Andreas Jenny
    Insert
    Wnk (Wnk Fly)
    Use
    Tags
    6xMyc
    Expression
    Insect
    Mutation
    RFxV mutated to AAxV
    Promoter
    Available since
    Oct. 4, 2023
    Availability
    Academic Institutions and Nonprofits only
    203980-plasmid-map-sequence-id-403221

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  8. pAG426GAL-FES

    Plasmid
    #203501
    Purpose
    Expresses human FES kinase from a GAL promoter with a URA3 marker
    Depositor
    Alejandro Chavez
    Insert
    FES (FES Human)
    Use
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    GAL1
    Available since
    Aug. 14, 2023
    Availability
    Academic Institutions and Nonprofits only
    203501-plasmid-map-sequence-id-402839

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  9. pAG416GAL10-c-Src

    Plasmid
    #203462
    Purpose
    Expresses human c-Src kinase from a GAL10 promoter with a URA3 marker
    Depositor
    Alejandro Chavez
    Insert
    c-Src (CSK Human)
    Use
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    GAL10
    Available since
    July 31, 2023
    Availability
    Academic Institutions and Nonprofits only
    203462-plasmid-map-sequence-id-402862

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  10. lenti-Cas9-Exo-Blast-BFP

    Plasmid
    #196721
    Purpose
    Sortable and selectable SpCas9 fused to exodeoxyribonuclease I (sbcB) from E. coli. For the creation of longer deletions.
    Depositor
    Bernhard Schmierer
    Insert
    Cas9-Exo1-T2A-BSD-TagBFP
    Use
    CRISPR and Lentiviral
    Tags
    Nucleoplasmin NLS and SV40 NLS
    Expression
    Mutation
    Promoter
    EF1a
    Available since
    April 12, 2023
    Availability
    Academic Institutions and Nonprofits only
    196721-plasmid-map-sequence-id-389753

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  11. pGB-06-07-Vps34

    Plasmid
    #187989
    Purpose
    Plasmid for the protein expression of Vps34 in a bacterial and insect expression system. Internal_Reference: SMC1323
    Depositor
    Sascha Martens
    Insert
    Vps34 (PIK3C3 Human)
    Use
    Tags
    Expression
    Bacterial and Insect
    Mutation
    Promoter
    Available since
    Feb. 7, 2023
    Availability
    Academic Institutions and Nonprofits only
    187989-plasmid-map-sequence-id-374438

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  12. pAIO-Ef1a-PE2-GFP:KCNQ2-C201R_pp

    Plasmid
    #185061
    Purpose
    Ef1a driven PE2 with pegRNA for editing C201R mutation (including silent PAM mutation) in KCNQ2. See Addgene plasmid #184445
    Depositor
    Bobby Koeleman
    Insert
    U6:pegRNA:scaffold:PBS+RT template
    Use
    CRISPR and Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    U6
    Available since
    Nov. 7, 2022
    Availability
    Academic Institutions and Nonprofits only
    185061-plasmid-map-sequence-id-376807

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  13. p2151 pHAGE-hSPC-TFEB-TagBFP-W

    Plasmid
    #188722
    Purpose
    Lentiviral vector allowing for SFTPC-driven expression of TFEB:tagBFP fusion protein
    Depositor
    Darrell Kotton
    Insert
    TFEB (TFEB Human)
    Use
    Lentiviral
    Tags
    tagBFP
    Expression
    Mutation
    Promoter
    hSP-C
    Available since
    Sept. 22, 2022
    Availability
    Academic Institutions and Nonprofits only
    188722-plasmid-map-sequence-id-372785

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  14. pCW57.1-GFP_mmu-miR-292b-3p_sponge

    Plasmid
    #132622
    Purpose
    Expresses a TurboGFP transcript with test microRNA sponge for mmu-miR-292b-3p as 3'UTR; serves as template for other microRNA sponges
    Depositor
    Constance Ciaudo
    Insert
    stop codon, mmu-miR-292b-3p sponge sequence and polyA signal
    Use
    Lentiviral; Doxycycline inducible
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    TRE
    Available since
    Aug. 15, 2022
    Availability
    Academic Institutions and Nonprofits only
    132622-plasmid-map-sequence-id-259572

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  15. mT2A-TagRFP-T-linker-dre-PBD*-FNF

    Plasmid
    #182342
    Purpose
    Recombineering cassette plasmid for T2A-TagRFP-T-linker-dre-PBD*-FNF
    Depositor
    Mario Capecchi
    Insert
    T2A-TagRFP-T-linker-dre-PBD*-FNF
    Use
    Mouse Targeting
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    March 31, 2022
    Availability
    Academic Institutions and Nonprofits only
    182342-plasmid-map-sequence-id-358194

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  18. CNN3A-c007

    Plasmid
    #175457
    Purpose
    Protein expression in bacterial cells. Lacking N-terminal 10 residues, G11-Y329.
    Depositor
    Opher Gileadi
    Insert
    CNN3 (CNN3 Human)
    Use
    Tags
    His6-SUMO
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Jan. 27, 2022
    Availability
    Academic Institutions and Nonprofits only
    175457-plasmid-map-sequence-id-353125

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  19. pLV V5-Eva1b

    Plasmid
    #175158
    Purpose
    Lentiviral expression of V5-tagged mouse Eva1b
    Depositor
    Larry Gerace
    Insert
    Eva1b (Eva1b Mouse)
    Use
    Lentiviral
    Tags
    V5
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Jan. 18, 2022
    Availability
    Academic Institutions and Nonprofits only
    175158-plasmid-map-sequence-id-352269

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  20. pLV Cenpv-V5

    Plasmid
    #175125
    Purpose
    Lentiviral expression of mouse Cenpv-V5
    Depositor
    Larry Gerace
    Insert
    Cenpv (Cenpv Mouse)
    Use
    Lentiviral
    Tags
    V5
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Jan. 12, 2022
    Availability
    Academic Institutions and Nonprofits only
    175125-plasmid-map-sequence-id-351968

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  21. pX330.orup

    Plasmid
    #110404
    Purpose
    CRISPR/Cas9 vector with sgRNA expression and puromycin resistance
    Depositor
    Sandra Martha Gomes Dias
    Type
    Empty backbone
    Use
    CRISPR
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CBh
    Available since
    Feb. 23, 2021
    Availability
    Academic Institutions and Nonprofits only
    110404-plasmid-map-sequence-id-307700

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  22. Clim Act MCH (CAM)

    Plasmid
    #104443
    Purpose
    Expresses mCherry under endogenous actin promoter. It can be used to transfect Corallochytrium limacisporum (Corallochytrea) cells and visualize them by fluorescent microscope.
    Depositor
    Inaki Ruiz-Trillo, Elena Casacuberta
    Insert
    mCherry
    Use
    Expression in corallochytrium (single-celled euka…
    Tags
    Expression
    Mutation
    Promoter
    actin
    Available since
    Feb. 17, 2021
    Availability
    Academic Institutions and Nonprofits only
    104443-plasmid-map-sequence-id-200869

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  23. Clim EF1 MCH (CEM)

    Plasmid
    #104444
    Purpose
    Expresses mCherry under elongation factor 1 promoter. It can be used to transfect Corallochytrium limacisporum (Corallochytrea) cells and visualize them by fluorescent microscope.
    Depositor
    Inaki Ruiz-Trillo, Elena Casacuberta
    Insert
    mCherry
    Use
    Expression in corallochytrium (single-celled euka…
    Tags
    Expression
    Mutation
    Promoter
    EF1
    Available since
    Feb. 17, 2021
    Availability
    Academic Institutions and Nonprofits only
    104444-plasmid-map-sequence-id-200864

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  24. Clim Tub MCH (CTM)

    Plasmid
    #104445
    Purpose
    Expresses mCherry under tubulin promoter. It can be used to transfect Corallochytrium limacisporum (Corallochytrea) cells and visualize them by fluorescent microscope.
    Depositor
    Inaki Ruiz-Trillo, Elena Casacuberta
    Insert
    mCherry
    Use
    Expression in corallochytrium (single-celled euka…
    Tags
    Expression
    Mutation
    Promoter
    tubulin promoter
    Available since
    Feb. 17, 2021
    Availability
    Academic Institutions and Nonprofits only
    104445-plasmid-map-sequence-id-200865

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  25. Lentiviral human Citron shRNA 1 GFP

    Plasmid
    #155286
    Purpose
    Lentiviral expression of human CIT shRNA, GFP expression, based on Addgene 12247
    Depositor
    Martin Carroll
    Insert
    Citron (CIT Human)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Aug. 31, 2020
    Availability
    Academic Institutions and Nonprofits only
    155286-plasmid-map-sequence-id-310000

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  26. pHAGE-hSPC-TFEB(delta30)eGFP-W

    Plasmid
    #126693
    Purpose
    Lineage specific over expression of a mutated TFEB in lung alveolar epithelial cells, where TFEB is missing the first 30 amino acids of the sequence.
    Depositor
    Darrell Kotton
    Insert
    TFEB(delta30) (TFEB Human)
    Use
    Lentiviral
    Tags
    Expression
    Mutation
    Deleted amino acids 1-30, H32D, Deleted Stop Codo…
    Promoter
    hSPC
    Available since
    July 9, 2020
    Availability
    Academic Institutions and Nonprofits only
    126693-plasmid-map-sequence-id-245693

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  27. pLL1273

    Plasmid
    #129288
    Purpose
    Expression plasmid with pLL1270 as backbone and alpha-Xylp_1211 and alpha-L-Galp_687 as inserts
    Depositor
    Lee Lynd
    Inserts
    alpha-Xylp_1211 (Ga0256695_ 1211)
    alpha-L-Galp_687 (Ga0256695_ 0687)
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Clostridium thermocellum DSM1313 Cellobiose Phosp…
    Available since
    March 19, 2020
    Availability
    Academic Institutions and Nonprofits only
    129288-plasmid-map-sequence-id-261300

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  28. pLL1274

    Plasmid
    #129385
    Purpose
    Expression plasmid with pLL1270 as backbone and alpha-Xylp_1211 and alpha-L-Araf_996 as inserts
    Depositor
    Lee Lynd
    Inserts
    alpha-Xylp_1211 (Ga0256695_ 1211)
    alpha-L-AraF_996 (Ga0256695_ 0996)
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Clostridium thermocellum DSM1313 Cellobiose Phosp…
    Available since
    March 19, 2020
    Availability
    Academic Institutions and Nonprofits only
    129385-plasmid-map-sequence-id-260448

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  29. Myc var1 K51/52 to NQ

    Plasmid
    #127287
    Purpose
    Expresses Myc with putative Ub site mutated
    Depositor
    Christine Milcarek
    Insert
    Myc (NM_001177352) (Myc Mouse)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    K51 and 52 to N and Q by Q5 mutagenesis
    Promoter
    CMV
    Available since
    Nov. 14, 2019
    Availability
    Academic Institutions and Nonprofits only
    127287-plasmid-map-sequence-id-250022

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  30. ELL3 Streptag CtoA

    Plasmid
    #127283
    Purpose
    Expresses mouse ELL3 with streptactin tag; To interrogate if the mutation decreases putative Myc activity
    Depositor
    Christine Milcarek
    Insert
    ELL3 (NM_145973) (ELL3 Mouse)
    Use
    Tags
    Twin strep-tag
    Expression
    Mammalian
    Mutation
    CEYLHNK to AEYLHQK
    Promoter
    EF1a
    Available since
    Aug. 7, 2019
    Availability
    Academic Institutions and Nonprofits only
    127283-plasmid-map-sequence-id-250049

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  31. ELL1 Streptag

    Plasmid
    #127278
    Purpose
    Expresses mouse ELL1 with streptactin tag in mammalian cells
    Depositor
    Christine Milcarek
    Insert
    ELL1 (NM_007924) (ELL Mouse)
    Use
    Tags
    Twin strep-tag
    Expression
    Mammalian
    Mutation
    Promoter
    EF1a
    Available since
    July 15, 2019
    Availability
    Academic Institutions and Nonprofits only
    127278-plasmid-map-sequence-id-250019

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  32. Myc var1 K397R

    Plasmid
    #127285
    Purpose
    Expresses Myc with putative Ub site mutated
    Depositor
    Christine Milcarek
    Insert
    Myc (NM_001177352) (Myc Mouse)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    K397R by Q5 mutagenesis
    Promoter
    CMV
    Available since
    July 15, 2019
    Availability
    Academic Institutions and Nonprofits only
    127285-plasmid-map-sequence-id-249995

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  33. Myc var1

    Plasmid
    #127284
    Purpose
    Expresses Myc to see if degraded by ELLs
    Depositor
    Christine Milcarek
    Insert
    Myc (NM_001177352) (Myc Mouse)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    transcript variant 1
    Promoter
    CMV
    Available since
    July 15, 2019
    Availability
    Academic Institutions and Nonprofits only
    127284-plasmid-map-sequence-id-249994

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  34. Myc var 1 K396/397 to NQ

    Plasmid
    #127286
    Purpose
    Expresses Myc with putative Ub site mutated
    Depositor
    Christine Milcarek
    Insert
    Myc (NM_001177352) (Myc Mouse)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    K396 and 397 to N and Q by Q5 mutagenesi
    Promoter
    CMV
    Available since
    July 15, 2019
    Availability
    Academic Institutions and Nonprofits only
    127286-plasmid-map-sequence-id-249993

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  35. pHH0103 UTROPHIN WW domain

    Plasmid
    #104275
    Purpose
    Bacterial expression of WW domain from UTROPHIN
    Depositor
    Sachdev Sidhu, Marius Sudol
    Insert
    UTROPHIN WW domain (UTRN Human)
    Use
    Tags
    GST
    Expression
    Bacterial
    Mutation
    codon optimized for expression in bacteria
    Promoter
    tac
    Available since
    Aug. 28, 2018
    Availability
    Academic Institutions and Nonprofits only
    104275-plasmid-map-sequence-id-203220

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  36. pHH0103 KIAA1688 WW domain #1

    Plasmid
    #104277
    Purpose
    Bacterial expression of WW domain #1 from KIAA1688
    Depositor
    Sachdev Sidhu, Marius Sudol
    Insert
    KIAA1688 WW domain #1 (ARHGAP39 Human)
    Use
    Tags
    GST
    Expression
    Bacterial
    Mutation
    codon optimized for expression in bacteria
    Promoter
    tac
    Available since
    Aug. 28, 2018
    Availability
    Academic Institutions and Nonprofits only
    104277-plasmid-map-sequence-id-202577

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  37. pHH0103 KIAA1688 WW domain #2

    Plasmid
    #104278
    Purpose
    Bacterial expression of WW domain #2 from KIAA1688
    Depositor
    Sachdev Sidhu, Marius Sudol
    Insert
    KIAA1688 WW domain #2 (ARHGAP39 Human)
    Use
    Tags
    GST
    Expression
    Bacterial
    Mutation
    codon optimized for expression in bacteria
    Promoter
    tac
    Available since
    Aug. 28, 2018
    Availability
    Academic Institutions and Nonprofits only
    104278-plasmid-map-sequence-id-203477

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  38. pHH0103 IQGAP2 WW domain

    Plasmid
    #104280
    Purpose
    Bacterial expression of WW domain from IQGAP2
    Depositor
    Sachdev Sidhu, Marius Sudol
    Insert
    IQGAP2 WW domain (IQGAP2 Human)
    Use
    Tags
    GST
    Expression
    Bacterial
    Mutation
    codon optimized for expression in bacteria
    Promoter
    tac
    Available since
    Aug. 28, 2018
    Availability
    Academic Institutions and Nonprofits only
    104280-plasmid-map-sequence-id-203486

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  39. pHH0103 ARHGAP27 WW domain #1

    Plasmid
    #104282
    Purpose
    Bacterial expression of WW domain #1 from ARHGAP27
    Depositor
    Sachdev Sidhu, Marius Sudol
    Insert
    ARHGAP27 WW domain #1 (ARHGAP27 Human)
    Use
    Tags
    GST
    Expression
    Bacterial
    Mutation
    codon optimized for expression in bacteria
    Promoter
    tac
    Available since
    Aug. 28, 2018
    Availability
    Academic Institutions and Nonprofits only
    104282-plasmid-map-sequence-id-203481

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  40. pHH0103 ARHGAP27 WW domain #2

    Plasmid
    #104283
    Purpose
    Bacterial expression of WW domain #2 from ARHGAP27
    Depositor
    Sachdev Sidhu, Marius Sudol
    Insert
    ARHGAP27 WW domain #2 (ARHGAP27 Human)
    Use
    Tags
    GST
    Expression
    Bacterial
    Mutation
    codon optimized for expression in bacteria
    Promoter
    tac
    Available since
    Aug. 28, 2018
    Availability
    Academic Institutions and Nonprofits only
    104283-plasmid-map-sequence-id-203211

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Showing: 4881 - 4920 of 8387 results