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pFTK038

Plasmid

#171310

Purpose

Part Plasmid Entry Vector (LVL0) from the Fungal Modular Cloning ToolKit.

Depositor

Arnold Driessen

Article

Mozsik et al ACS Synth Biol. 2021 Nov 2. doi: 10.1021

Insert

hph, hygR, hygromycin selection marker

Use

Synthetic Biology

Expression

Bacterial and Yeast

Mutation

n/a

Available Since

Dec. 14, 2021

Availability

Academic Institutions and Nonprofits only

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pFTK037

Plasmid

#171309

Purpose

Part Plasmid Entry Vector (LVL0) from the Fungal Modular Cloning ToolKit.

Depositor

Arnold Driessen

Article

Mozsik et al ACS Synth Biol. 2021 Nov 2. doi: 10.1021

Insert

bleoR phleomycin, selection marker

Use

Synthetic Biology

Expression

Bacterial and Yeast

Mutation

n/a

Available Since

Dec. 14, 2021

Availability

Academic Institutions and Nonprofits only

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pZA2[PLac:mCherry,PTAN:GFP]

Plasmid

#60780

Purpose

Contains PLac driving expression of mCherry and PTAN driving expression of GFP.

Depositor

Matthew Bennett

Article

Shis et al ACS Synth Biol. 2014 Sep 19;3(9):645-51.

Inserts

mCherry
yemGFP

Available Since

April 30, 2015

Availability

Academic Institutions and Nonprofits only

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TDGS50

Plasmid

#183189

Purpose

TEV protease expressed under J23106 promoter; DivIVAsfGFP-TEV protease recognition site-M0050 expressed under J23116 promoter

Depositor

Hsiao-Chun Huang

Article

Hong et al ACS Synth Biol. 2021 Aug 20;10(8):1830-1

Inserts

DivIVA-GFP(tevR M0050)
TEV-S219V

Expression

Bacterial

Available Since

July 12, 2022

Availability

Academic Institutions and Nonprofits only

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pFTK084

Plasmid

#171356

Purpose

Part Plasmid Entry Vector (LVL0) from the Fungal Modular Cloning ToolKit.

Depositor

Arnold Driessen

Article

Mozsik et al ACS Synth Biol. 2021 Nov 2. doi: 10.1021

Insert

TtRNA[Met] A.niger Pol-III terminator

Use

Synthetic Biology

Expression

Bacterial and Yeast

Mutation

n/a

Available Since

Jan. 7, 2022

Availability

Academic Institutions and Nonprofits only

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pFTK039

Plasmid

#171311

Purpose

Part Plasmid Entry Vector (LVL0) from the Fungal Modular Cloning ToolKit.

Depositor

Arnold Driessen

Article

Mozsik et al ACS Synth Biol. 2021 Nov 2. doi: 10.1021

Insert

pyrG AO090011000868 Orotidine 5'-phosphate decarboxylase, selection marker

Use

Synthetic Biology

Expression

Bacterial and Yeast

Mutation

n/a

Available Since

Dec. 14, 2021

Availability

Academic Institutions and Nonprofits only

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pUCSh3-mRFP

Plasmid

#170983

Purpose

Encodes sgRNA scaffold with mRFP in place of target and Tetracycline resistance transposon. New targets can be cloned replacing mRFP using around the horn cloning.

Depositor

Christopher Reisch

Article

Trujillo Rodriguez et al ACS Synth Biol. 2023 Jun 27. doi: 10.102

Insert

mRFP

Use

CRISPR and Synthetic Biology

Expression

Bacterial

Promoter

J23119

Available Since

July 14, 2023

Availability

Academic Institutions and Nonprofits only

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pFTK080

Plasmid

#171352

Purpose

Part Plasmid Entry Vector (LVL0) from the Fungal Modular Cloning ToolKit.

Depositor

Arnold Driessen

Article

Mozsik et al ACS Synth Biol. 2021 Nov 2. doi: 10.1021

Insert

Tcyc1 YJR048W terminator

Use

Synthetic Biology

Expression

Bacterial and Yeast

Mutation

n/a

Available Since

Jan. 5, 2022

Availability

Academic Institutions and Nonprofits only

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pFTK079

Plasmid

#171351

Purpose

Part Plasmid Entry Vector (LVL0) from the Fungal Modular Cloning ToolKit.

Depositor

Arnold Driessen

Article

Mozsik et al ACS Synth Biol. 2021 Nov 2. doi: 10.1021

Insert

Toat1 Pc18g03600 terminator

Use

Synthetic Biology

Expression

Bacterial and Yeast

Mutation

n/a

Available Since

Jan. 5, 2022

Availability

Academic Institutions and Nonprofits only

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pFTK052

Plasmid

#171324

Purpose

Part Plasmid Entry Vector (LVL0) from the Fungal Modular Cloning ToolKit.

Depositor

Arnold Driessen

Article

Mozsik et al ACS Synth Biol. 2021 Nov 2. doi: 10.1021

Insert

Firefly luciferase reporter

Use

Synthetic Biology

Expression

Bacterial and Yeast

Mutation

n/a

Available Since

Jan. 3, 2022

Availability

Academic Institutions and Nonprofits only

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pFTK049

Plasmid

#171321

Purpose

Part Plasmid Entry Vector (LVL0) from the Fungal Modular Cloning ToolKit.

Depositor

Arnold Driessen

Article

Mozsik et al ACS Synth Biol. 2021 Nov 2. doi: 10.1021

Insert

mCherry fluorescent reporter

Use

Synthetic Biology

Expression

Bacterial and Yeast

Mutation

n/a

Available Since

Dec. 22, 2021

Availability

Academic Institutions and Nonprofits only

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pENTR APLP2 shRNA 3

Plasmid

#30161

Depositor

Dennis Selkoe, Tracy Young-Pearse

Article

Young-Pearse et al Neural Dev. 2008 Jun 23;3:15. doi: 10.11

Insert

APLP2 shRNA 3

Expression

Mammalian

Available Since

July 28, 2011

Availability

Academic Institutions and Nonprofits only
pFTK051

Plasmid

#171323

Purpose

Part Plasmid Entry Vector (LVL0) from the Fungal Modular Cloning ToolKit.

Depositor

Arnold Driessen

Article

Mozsik et al ACS Synth Biol. 2021 Nov 2. doi: 10.1021

Insert

eBFP fluorescent reporter

Use

Synthetic Biology

Expression

Bacterial and Yeast

Mutation

n/a

Available Since

Jan. 3, 2022

Availability

Academic Institutions and Nonprofits only

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pCG-A-sacB(SP)-B

Plasmid

#87370

Purpose

An ORF part-containing vector harbouring the signal peptide from SacB with 4 bp overhangs specific for the ‘signal peptide’ position within an ORF assembly

Depositor

Tom Ellis

Article

Gilbert et al ACS Synth Biol. 2017 Mar 7. doi: 10.1021

Insert

SacB signal peptide

Use

Synthetic Biology

Available Since

July 21, 2017

Availability

Academic Institutions and Nonprofits only

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msEGFP-PACR_pRSETB

Plasmid

#55775

Purpose

Genetically encoded caged Ca2+

Depositor

Takeharu Nagai

Article

Fukuda et al ACS Chem Biol. 2014 May 16;9(5):1197-203

Insert

PACR

Tags

6xHis and msEGFP

Expression

Bacterial

Promoter

T7 promoter

Available Since

July 3, 2014

Availability

Academic Institutions and Nonprofits only
pBP-TL4

Plasmid

#72988

Purpose

RBS_H5

Depositor

Paul Freemont

Article

Moore et al ACS Synth Biol. 2016 May 2.

Insert

H5 RBS

Use

Synthetic Biology

Mutation

CAT gene - C435G (nucleotide) - silent mutagenesi…

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only
pBP-TL8

Plasmid

#72992

Purpose

RBS_L1

Depositor

Paul Freemont

Article

Moore et al ACS Synth Biol. 2016 May 2.

Insert

L1 RBS

Use

Synthetic Biology

Mutation

CAT gene - C435G (nucleotide) - silent mutagenesi…

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only
pBP-L1U1H09

Plasmid

#73008

Purpose

L1U1H09 terminator

Depositor

Paul Freemont

Article

Moore et al ACS Synth Biol. 2016 May 2.

Insert

L1U1H09 terminator

Use

Synthetic Biology

Mutation

CAT gene - C435G (nucleotide) - silent mutagenesi…

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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pRSET B-BKAR

Plasmid

#14877

Depositor

Alexandra Newton

Article

Kunkel et al J Biol Chem. 2005 Feb 18. 280(7):5581-7.

Insert

B kinase activity reporter

Tags

CFP and YFP

Expression

Bacterial

Available Since

Aug. 9, 2007

Availability

Academic Institutions and Nonprofits only
pMulti-sgRNA-LacZ

Plasmid

#99913

Purpose

Receptor plasmid for the assembly of multiple sgRNAs

Depositor

Yujie Sun

Article

Shao et al ACS Synth Biol. 2017 Sep 7. doi: 10.1021

Type

Empty backbone

Use

CRISPR

Expression

Mammalian

Available Since

Oct. 10, 2017

Availability

Academic Institutions and Nonprofits only

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