We narrowed to 9,416 results for: Coli

1. pAJM.1642

   Plasmid

   #108535

   Purpose

   CinRAM + PCin-YFP Reporter. OHC14 sensor for use in non-Marionette strain.

   Depositor

   Christopher Voigt

   Article

   Meyer et al Nat Chem Biol. 2018 Nov 26. pii: 10.1038

   Insert

   CinRAM + PCin-YFP
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   CinR: S72C V80I G114D G115D R137H K220R A224T *24…

   Promoter

   Available Since

   Aug. 8, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. pNAS1B

   Plasmid

   #61968

   Purpose

   Duet vector for co-expression of GFP and mCherry from PBAD and PLtetO promoters, respectively; parent vector for split mCherry assembly vectors

   Depositor

   Lynne Regan

   Article

   Sawyer et al ACS Chem Biol. 2014 Nov 21;9(11):2502-7.

   Inserts

   mCherry
   GFP
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Serine 65 to Glycine (GGT), Serine 72 to Alanine …

   Promoter

   PBAD and PLtetO

   Available Since

   Jan. 30, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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3. pAJT194

   Plasmid

   #202293

   Purpose

   Type IIS cloning plasmid for LPattB5 integrations w/ OriT

   Depositor

   Christopher Voigt

   Article

   Triassi et al Cell Syst. 2023 Jun 21;14(6):512-524.e12

   Insert

   sfGFP
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   Sept. 18, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. pFRET-HSP33 cys

   Plasmid

   #16076

   Depositor

   Ulrich Hammerling

   Article

   Guzy et al Cell Metab. 2005 Jun;1(6):401-8. doi: 10

   Insert

   HSP33 cys
   

   Use

   Tags

   ECFP and EYFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Nov. 2, 2007

   Availability

   Academic Institutions and Nonprofits only

5. pFW2000

   Plasmid

   #224211

   Purpose

   E. coli-Bacteroides shuttle plasmid; pB8-51 (Bacteroides replication origin), p15A (E. coli replication origin)

   Depositor

   Ophelia Venturelli

   Article

   Feng et al Cell Host Microbe. 2022 Feb 9;30(2):200-

   Type

   Empty backbone

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   Sept. 11, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pMS17

   Plasmid

   #127088

   Purpose

   Integrating vector containing a tetracycline inducible promoter for use in Streptomyces sp.

   Depositor

   Maggie Smith

   Article

   Rodriguez-Garcia et al Nucleic Acids Res. 2005 May 25;33(9):e87

   Inserts

   tcp830
   TetRiS
   terminator mmrt
   Terminator fdt
   

   Use

   Streptomyce integration vector derived from phic3…

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   July 10, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. pLG018 (AVAST type 2)

   Plasmid

   #157896

   Purpose

   Expresses the AVAST type 2 defense system

   Depositor

   Feng Zhang

   Article

   Gao et al Science. 2020 Aug 28;369(6507):1077-1084

   Insert

   AVAST type 2 (STAND ATPase with uncharacterized helical N-terminal domain)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   Dec. 17, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. pEaaK-NanR-J23113-pNanA-CadC-pCadBA-GFP

   Plasmid

   #200277

   Purpose

   Characterisation of the sialic acid-responsive circuit with fluorescent reporter

   Depositor

   Matthew Chang

   Article

   Koh et al Nat Commun. 2022 Jul 4;13(1):3834. doi:

   Inserts

   CadC
   NanR
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   Aug. 1, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. pLG020 (AVAST type 4)

   Plasmid

   #157898

   Purpose

   Expresses the AVAST type 4 defense system

   Depositor

   Feng Zhang

   Article

   Gao et al Science. 2020 Aug 28;369(6507):1077-1084

   Insert

   AVAST type 4 (STAND ATPase with Mrr-like N-terminal domain)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   Dec. 16, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. pTU2S-a (p15A origin)

    Plasmid

    #74088

    Purpose

    Secondary module (BpiI) site in pTU2-a backbone with p15A (low-copy) origin of replication

    Depositor

    Paul Freemont

    Article

    Moore et al ACS Synth Biol. 2016 May 2.

    Insert

    Golden Gate destination vector - Level 2, 2 TU's with secondary module and p15A origin
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    None

    Available Since

    April 30, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. pMBA334

    Plasmid

    #214746

    Purpose

    High copy thyA-infA plasmid containing gfp and no antibiotic resistance gene (ARG)

    Depositor

    Tae Seok Moon

    Article

    Amrofell et al Cell Rep Methods. 2023 Dec 18;3(12):1006

    Insert

    BBa_J23119-RiboJ-RBS(21992)-gfpmut3
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    May 13, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. pAT222

    Plasmid

    #40942

    Depositor

    Angela Taddei

    Article

    Dubarry et al Genes Dev. 2011 Jul 1;25(13):1365-70.

    Insert

    GFP-LacI**
    

    Use

    Tags

    GFP(S65T, V163A) and SV40 NLS

    Expression

    Yeast

    Mutation

    LacI**/LacI2G(4 mutations) (see comment below); l…

    Promoter

    His3 promoter fragment

    Available Since

    Feb. 12, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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13. placZα-tdMCP

    Plasmid

    #191132

    Purpose

    Expression of tdMCP from a lacZα inducible promoter

    Depositor

    Ariel Lindner

    Article

    Guo et al Cell. 2022 Sep 29;185(20):3823-3837.e23.

    Insert

    dMCP_1
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    lac-inducible promoter

    Available Since

    June 12, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. pLV-EF1a-MinE(L4E)-EGFP_IRES-Blast

    Plasmid

    #213117

    Purpose

    Expression of a faster-running MinE mutant in mammalian cells

    Depositor

    Scott Coyle

    Article

    Rajasekaran et al Cell. 2023 Dec 27:S0092-8674(23)01339-9.

    Insert

    MinE(L4E)-EGFP (minE Escherichia coli)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    L4E

    Promoter

    EF1a

    Available Since

    Jan. 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. pLV-EF1a-FRB-mCherry-MinD-IRES-Puro

    Plasmid

    #213116

    Purpose

    Expression of FRB-mCherry-MinD in mammalian cells for payload delivery

    Depositor

    Scott Coyle

    Article

    Rajasekaran et al Cell. 2023 Dec 27:S0092-8674(23)01339-9.

    Insert

    FRB-mCherry-MinD (minD Escherichia coli)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available Since

    Jan. 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. pMVA

    Plasmid

    #121149

    Purpose

    This plamid was used for improving the production of DMAPP (IPP) in E.coli

    Depositor

    Minjuan Xu

    Article

    He et al ACS Synth Biol. 2018 Sep 21;7(9):2094-21

    Insert

    MVA Pathway (ERG12, ERG8, MVD1, IDI, MvaE, MvaS )
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    June 5, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCHA1.1-Rap2a-V5-Hygro

    Plasmid

    #195510

    Purpose

    lentiviral vector expressing RAP2A CDS with hygromycin selection

    Depositor

    Joanna Wysocka

    Article

    Bajpai et al Science. 2023 Aug 11;381(6658):eade6289.

    Insert

    Hygromycin
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hPGK

    Available Since

    Feb. 8, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. pBEL2511

    Plasmid

    #195625

    Purpose

    Complementation of MlaA(WT)

    Depositor

    Gira Bhabha, Damian Ekiert

    Article

    MacRae et al J Biol Chem. 2023 Apr 24:104744. doi: 10

    Insert

    MlaA
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    May 2, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pAJT195

    Plasmid

    #202294

    Purpose

    Type IIS cloning plasmid for LPattB7 integrations w/ OriT

    Depositor

    Christopher Voigt

    Article

    Triassi et al Cell Syst. 2023 Jun 21;14(6):512-524.e12

    Insert

    sfGFP
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Sept. 18, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pJB042 (FtsZ-mEos2)

    Plasmid

    #49764

    Purpose

    Inducible expression of FtsZ-mEos2 in bacteria

    Depositor

    Jie Xiao

    Article

    Buss et al Mol Microbiol. 2013 Sep;89(6):1099-120.

    Insert

    FtsZ-mEos2
    

    Use

    Tags

    mEos2

    Expression

    Bacterial

    Mutation

    Promoter

    T5-lac

    Available Since

    March 7, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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21. pISFba1

    Plasmid

    #226820

    Purpose

    Expressing ISEre1 controled by pCas promoter, λ-red recombinase, SacB and ISFba1 guide RNA targeting pMB1 ori

    Depositor

    Sheng Yang

    Article

    Zhou et al Biotechnol J. 2025 Mar;20(3):e70005. doi

    Insert

    ISFba1
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    March 27, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pISFba1reRNA

    Plasmid

    #226822

    Purpose

    Expressing ISFba1 guide RNA targeting maeB site controled by PJ23119

    Depositor

    Sheng Yang

    Article

    Zhou et al Biotechnol J. 2025 Mar;20(3):e70005. doi

    Insert

    ISFba1 none target guide RNA
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    March 27, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pSLS.491

    Plasmid

    #184956

    Purpose

    Test effect of a1/a2 length on editing rpoB using retron recombineering

    Depositor

    Seth Shipman

    Article

    Lopez et al Nat Chem Biol. 2022 Feb;18(2):199-206. d

    Insert

    Eco1 RT and recombineering ncRNA, rpoB T1534C, a1/a2 length: 12
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    rpoB donor T1534C

    Promoter

    T7/lac

    Available Since

    Nov. 2, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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24. J23100_GB

    Plasmid

    #65983

    Purpose

    MoClo Basic Part: Constitutive promoter - Anderson series - high strength [G:J23100:B]

    Depositor

    Douglas Densmore

    Article

    Iverson et al ACS Synth Biol. 2016 Jan 15;5(1):99-103.

    Insert

    Constitutive promoter
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Jan. 21, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. P823_eSpCas9(1.1)-RecA

    Plasmid

    #87264

    Purpose

    Human codon optimized RecA protein was fused to eSpCas9(1.1) for enhanced genome editing efficiency

    Depositor

    Yonglun Luo

    Article

    Lin et al J Biotechnol. 2017 Mar 1;247:42-49. doi:

    Insert

    RecA
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CBh

    Available Since

    May 3, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pFW5000

    Plasmid

    #224214

    Purpose

    E. coli-Bacteroides shuttle plasmid; pB8-51 (Bacteroides replication origin), p15A (E. coli replication origin), tetQ

    Depositor

    Ophelia Venturelli

    Article

    Feng et al Cell Host Microbe. 2022 Feb 9;30(2):200-

    Type

    Empty backbone

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Sept. 11, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pcDNA3.1-subtilase A

    Plasmid

    #35948

    Depositor

    Zhaoyang Feng

    Article

    Yuan et al PLoS One. 2011;6(8):e22354. Epub 2011 Au

    Insert

    Subtilase A (subA E. Coli)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Nov. 14, 2012

    Availability

    Academic Institutions and Nonprofits only

28. pSCL.139

    Plasmid

    #184994

    Purpose

    Express Cas9-P2A-Eco1RT in human cells

    Depositor

    Seth Shipman

    Article

    Lopez et al Nat Chem Biol. 2022 Feb;18(2):199-206. d

    Insert

    SpCas9-P2A-Eco1RT
    

    Use

    Tags

    SV40NLS

    Expression

    Mammalian

    Mutation

    Human codon optimized RT

    Promoter

    TetOn-3g

    Available Since

    Nov. 3, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pPBCAG-rtTAM2-IH

    Plasmid

    #140827

    Purpose

    Expresses rtTAM2-IRES-HygR in mammalian cells

    Depositor

    Hideyuki Okano

    Article

    Kisa et al Stem Cell Reports. 2017 Dec 12;9(6):1825

    Inserts

    rtTAM2
    HygR
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    July 28, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pMBA328

    Plasmid

    #214748

    Purpose

    Medium copy glmS-nadE plasmid containing mCherry and no antibiotic resistance gene (ARG)

    Depositor

    Tae Seok Moon

    Article

    Amrofell et al Cell Rep Methods. 2023 Dec 18;3(12):1006

    Insert

    BBa_J23119-RBS(22821)-mcherry
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    June 6, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge