We narrowed to 9,260 results for: Coli

1. pCRIS

   Plasmid

   #120424

   Purpose

   This plasmid encodes the complete CRISPR/dCas9 machinery for repressing transposition of the following bacterial Insertion Sequences: IS1, IS3, IS5, and IS150.

   Depositor

   Tamás Fehér

   Article

   Nyerges et al Synth Biol (Oxf). 2019;4(1):ysz008. doi:

   Insert

   CRISPR spacers targeting IS1, IS5, IS3, IS150
   

   Use

   CRISPR and Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   constitutive

   Available since

   Jan. 17, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCAG-AILR-LplA-ER

   Plasmid

   #42574

   Depositor

   Alice Ting

   Article

   Liu et al ACS Nano. 2012 Dec 21;6(12):11080-7. doi

   Insert

   LplA(AILR) with ER-retention sequence KDEL
   

   Use

   Tags

   FLAG

   Expression

   Mutation

   Compared to wild-type LplA: tryptophan 37 to alan…

   Promoter

   CAG

   Available since

   April 11, 2013

   Availability

   Academic Institutions and Nonprofits only

3. pAL612

   Plasmid

   #119860

   Purpose

   precusor outer membrane protein A, ompA, with mutations Cysteine 290 Serine, Glycine 244 Cysteine

   Depositor

   Linda Randall

   Article

   Bariya et al J Bacteriol. 2018 Oct 1. pii: JB.00493-1

   Insert

   outer membrane protein A, ompA, with mutations Cysterine 290 to Serine and Glycine 244 to Cysteine
   

   Use

   Tags

   none

   Expression

   Bacterial

   Mutation

   Cysterine 290 to Serine and Glycine 244 to Cystei…

   Promoter

   lac

   Available since

   Dec. 20, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. pETM41-EcLonA

   Plasmid

   #38330

   Depositor

   Florian Freimoser

   Article

   Werner et al Fungal Genet Biol. 2007 Sep;44(9):845-52

   Insert

   Lon protease fragment (lon Escherichia coli)
   

   Use

   Tags

   His, MBP, and TEV

   Expression

   Bacterial

   Mutation

   Polyphosphate binding fragment of Lon protease. …

   Promoter

   T7

   Available since

   Aug. 10, 2012

   Availability

   Academic Institutions and Nonprofits only

5. pAL725

   Plasmid

   #119862

   Purpose

   precusor D-galactose/methyl-galactoside ABC transporter periplasmic binding protein, mglB, L244C, D287C

   Depositor

   Linda Randall

   Article

   Bariya et al J Bacteriol. 2018 Oct 1. pii: JB.00493-1

   Insert

   D-galactose/methyl-galactoside ABC transporter periplasmic binding protein, mglB, L244C, D287C (mglB Escherichia coli str. K-12)
   

   Use

   Tags

   none

   Expression

   Bacterial

   Mutation

   Leucine 244 to Cysteine, Aspartate 287 to Cysteine

   Promoter

   T5

   Available since

   Dec. 20, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. pcDNA3.1 FLIPE-10u

   Plasmid

   #13542

   Depositor

   Wolf Frommer

   Article

   Okumoto et al Proc Natl Acad Sci U S A. 2005 Jun 14. 1

   Insert

   FLIP-E A207R
   

   Use

   Tags

   ECFP and Venus

   Expression

   Mammalian

   Mutation

   A207R in binding protein

   Promoter

   Available since

   Dec. 15, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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7. pDisplay FLIPE-1m

   Plasmid

   #13548

   Depositor

   Wolf Frommer

   Article

   Okumoto et al Proc Natl Acad Sci U S A. 2005 Jun 14. 1

   Insert

   FLIP-E A207W
   

   Use

   Tags

   ECFP and Venus

   Expression

   Mammalian

   Mutation

   A207W in binding protein

   Promoter

   Available since

   Dec. 15, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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8. P3U2C5T2-v1-2 (Pl-lacO1-tetR) (136p)

   Plasmid

   #70212

   Purpose

   1 Part of 4-part switch

   Depositor

   Richard Murray

   Article

   Sun et al ACS Synth Biol. 2014 Jun 20;3(6):387-97.

   Insert

   tetR
   

   Use

   Tags

   Expression

   Mutation

   Promoter

   Available since

   May 19, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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9. pDisplay FLIPE-10u

   Plasmid

   #13546

   Depositor

   Wolf Frommer

   Article

   Okumoto et al Proc Natl Acad Sci U S A. 2005 Jun 14. 1

   Insert

   FLIP-E A207R
   

   Use

   Tags

   ECFP and Venus

   Expression

   Mammalian

   Mutation

   A207R in binding protein

   Promoter

   Available since

   Dec. 15, 2006

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pSR26-2

    Plasmid

    #104335

    Purpose

    Expresses ompR from a TetR reuglated promoter under inducible ATC control.

    Depositor

    Jeffrey Tabor

    Article

    Landry et al Nat Commun. 2018 Apr 12;9(1):1433. doi:

    Insert

    OmpR (ompR Escherichia coli str. K-12 substr. MG1655)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    PLTetO1

    Available since

    April 17, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. pET28a-HsSENP5

    Plasmid

    #71468

    Purpose

    Encodes human full length SENP5 optimised for E. coli expression. Suitable for in vitro transcription/translation (T7).

    Depositor

    Jorge Eduardo Azevedo

    Article

    Mendes et al Biochim Biophys Acta. 2015 Oct 29. pii:

    Insert

    SUMO1/sentrin specific peptidase 5 (SENP5 Human)
    

    Use

    Tags

    Hexahistidine

    Expression

    Bacterial

    Mutation

    Gene optimised for E. coli expression (Genscript)

    Promoter

    T7

    Available since

    Dec. 10, 2015

    Availability

    Academic Institutions and Nonprofits only

12. pKDC.015

    Plasmid

    #184992

    Purpose

    Express -Eco2 RT and ncRNA in human cells

    Depositor

    Seth Shipman

    Article

    Lopez et al Nat Chem Biol. 2022 Feb;18(2):199-206. d

    Insert

    Eco2: RT and ncRNA(wt), a1/a2 length: 13
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Human codon optimized RT

    Promoter

    TetOn-3g

    Available since

    Nov. 3, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. pRSET FLIPE-10u

    Plasmid

    #13538

    Depositor

    Wolf Frommer

    Article

    Okumoto et al Proc Natl Acad Sci U S A. 2005 Jun 14. 1

    Insert

    FLIP-E A207R
    

    Use

    Tags

    ECFP and Venus

    Expression

    Bacterial

    Mutation

    A207R in binding protein

    Promoter

    Available since

    Dec. 15, 2006

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pRSET FLIPE-1m

    Plasmid

    #13540

    Depositor

    Wolf Frommer

    Article

    Okumoto et al Proc Natl Acad Sci U S A. 2005 Jun 14. 1

    Insert

    FLIP-E A207W
    

    Use

    Tags

    ECFP and Venus

    Expression

    Bacterial

    Mutation

    A207W in binding protein

    Promoter

    Available since

    Dec. 15, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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15. pPJ174(JPUB_006779)

    Plasmid

    #83827

    Purpose

    Carries operon 1 final

    Depositor

    Harry Beller

    Article

    Javidpour et al PLoS One. 2016 Mar 14;11(3):e0151087. do

    Inserts

    Kuste3603
    kuste3605
    kuste3606
    

    Use

    Pfab217 (p15a ori, kanr)

    Tags

    Expression

    Mutation

    Promoter

    op1_3603_for, op1_3605_for, and op1_3606_for

    Available since

    Oct. 20, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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16. EC.SurA.TYB1.a172-274.wt

    Plasmid

    #15934

    Depositor

    David McKay

    Article

    Xu et al J Mol Biol. 2007 Oct 19. 373(2):367-81.

    Insert

    Peptidyl prolyl isomerase domain 1 of SurA (surA E. coli)
    

    Use

    Tags

    intein

    Expression

    Bacterial

    Mutation

    wild type

    Promoter

    Available since

    Nov. 9, 2007

    Availability

    Academic Institutions and Nonprofits only

    

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17. pRSET FLIPglu-600uDelta11 Aphrodite

    Plasmid

    #13569

    Depositor

    Wolf Frommer

    Article

    Deuschle et al Plant Cell. 2006 Sep . 18(9):2314-25.

    Insert

    FLIPglu F16A Delta11 Aphrodite
    

    Use

    Tags

    Aphrodite and ECFP

    Expression

    Bacterial

    Mutation

    Fluorophore-derived linker shortened. F16A mutati…

    Promoter

    Available since

    March 2, 2007

    Availability

    Academic Institutions and Nonprofits only

    

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18. pFLIPglu-3.2mDelta13

    Plasmid

    #12992

    Depositor

    Wolf Frommer

    Article

    Deuschle et al Plant Cell. 2006 Sep . 18(9):2314-25.

    Insert

    FLIPglu F16A S112A Delta13
    

    Use

    Plant nanosensor

    Tags

    ECFP and EYFP

    Expression

    Plant

    Mutation

    F16A, S112A in binding protein

    Promoter

    Available since

    Jan. 5, 2007

    Availability

    Academic Institutions and Nonprofits only

19. pKDsgRNA-ldhA

    Plasmid

    #102288

    Purpose

    Contains arabinose-induced lambda Red and a tet-inducible gRNA targeting ldhA.

    Depositor

    Michael Lynch

    Article

    Moreb et al ACS Synth Biol. 2017 Oct 2. doi: 10.1021

    Insert

    ldhA gRNA
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    pTet

    Available since

    April 12, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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20. pKDsgRNA-rpsL

    Plasmid

    #89953

    Purpose

    Contains arabinose-induced lambda Red and a tet-inducible gRNA targeting rpsL.

    Depositor

    Michael Lynch

    Article

    Moreb et al ACS Synth Biol. 2017 Oct 2. doi: 10.1021

    Insert

    rpsL gRNA
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    pTet

    Available since

    June 19, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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21. pFLIPglu-170nDelta13

    Plasmid

    #12989

    Depositor

    Wolf Frommer

    Article

    Deuschle et al Plant Cell. 2006 Sep . 18(9):2314-25.

    Insert

    FLIPglu F16F Delta13
    

    Use

    Plant nanosensor

    Tags

    ECFP and EYFP

    Expression

    Plant

    Mutation

    WT binding protein (no F16A)

    Promoter

    Available since

    Jan. 5, 2007

    Availability

    Academic Institutions and Nonprofits only

22. pBP-pET-RBS

    Plasmid

    #72981

    Purpose

    PET RBS - derived from pET vectors (5' GTAC/3' CATA fusion)

    Depositor

    Paul Freemont

    Article

    Moore et al ACS Synth Biol. 2016 May 2.

    Insert

    pET RBS derived from pET vectors
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    CAT gene - C435G (nucleotide) - silent mutagenesi…

    Promoter

    Available since

    July 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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23. pDTnLAPP2A frame 0

    Plasmid

    #28226

    Depositor

    Harald von Melchner

    Article

    Schnutgen et al Nat Methods. 2011 Feb . 8(2):103-4.

    Insert

    Hygrophosphotransferase-P2A-Enhanced Green Fluorescent Protein
    

    Use

    Retroviral

    Tags

    EGFP and nLAP-tag

    Expression

    Mutation

    Promoter

    Available since

    Dec. 7, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. HL 818 (=HL 716 + pHL 98)

    Plasmid

    #30006

    Depositor

    Han Lim

    Article

    Hussein et al Proc Natl Acad Sci U S A. 2011 Jan 18;10

    Insert

    pLlacO-1:DsrA-mcherry, pLtetO-1:rpoS::gfp
    

    Use

    Synthetic Biology

    Tags

    mcherry, GFP

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Aug. 25, 2011

    Availability

    Academic Institutions and Nonprofits only

25. pETM6_Ptac_BmHps_BmPhi_BsMdh

    Plasmid

    #129103

    Purpose

    Expresses Mdh from Bacillus subtilis and Hps & Phi from Bacillus methanolicus

    Depositor

    Eleftherios Terry Papoutsakis

    Article

    Whitaker et al Metab Eng. 2017 Jan;39:49-59. doi: 10.10

    Insert

    BsMDH BmHPS BmPHI
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Nov. 11, 2019

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

26. pPB-DEST-R1R2-pNeo

    Plasmid

    #141057

    Purpose

    Mutisite Gateway mediated vector construction

    Depositor

    Hideyuki Okano

    Article

    Yoshimatsu et al PLoS One. 2019 Aug 27;14(8):e0221164. do

    Insert

    Neomycin resistance gene
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 7, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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27. pENTR-L1-PGK-Neo-L2

    Plasmid

    #141007

    Purpose

    Mutisite Gateway mediated vector construction

    Depositor

    Hideyuki Okano

    Article

    Yoshimatsu et al PLoS One. 2019 Aug 27;14(8):e0221164. do

    Insert

    Neomycin resistance gene
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pHCKan-yibDp-mdlC

    Plasmid

    #134590

    Purpose

    Low Phosphate inducible mdlC benzylformate decarboxylase

    Depositor

    Michael Lynch

    Article

    Menacho-Melgar et al Biotechnol Bioeng. 2020 May 22. doi: 10.

    Insert

    mdlC (benzylformate decarboxylase) C -terminal his tag
    

    Use

    Tags

    6x-His

    Expression

    Bacterial

    Mutation

    Promoter

    yibDp

    Available since

    May 5, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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29. JBEI-6823

    Plasmid

    #84986

    Purpose

    For expression of MevTaco-PMKco-MKco(aka 2B) under lacUV5 promoter for isopentenol production

    Depositor

    Taek Soon Lee

    Article

    George et al Sci Rep. 2015 Jun 8;5:11128. doi: 10.103

    Insert

    MevTaco-PMKco-Mkco
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    LacUV5

    Available since

    Feb. 27, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pNIC28-BSA4-G-S10

    Plasmid

    #127828

    Purpose

    Production of recombinant human ribosomal protein S10

    Depositor

    Ivanhoe Leung

    Article

    Correddu et al Sci Rep. 2019 Jun 20;9(1):8884. doi: 10.

    Insert

    RPS10 (RPS10 Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Aug. 5, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge