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Showing: 4921 - 4960 of 41926 results
  1. pSR657

    Plasmid
    #65214
    Purpose
    Baculovirus-mediated rAAV production: Expresses bicistronic AAV-2 rep gene and polycistronic AAV-2 cap gene in Sf9 insect cells
    Depositor
    Inserts
    modified AAV-2 rep78
    modified AAV-2 cap
    Use
    AAV
    Expression
    Insect
    Promoter
    p10 and polyhedrin
    Available Since
    June 17, 2015
    Availability
    Academic Institutions and Nonprofits only
  2. pAD-KPS12-Pman

    Plasmid
    #133832
    Purpose
    Fluorescent protein mKate(KPS12) variant for optimized expression in Bacillus mycoides; under control of mannose inducible promoter.
    Depositor
    Insert
    mKate(KPS12)
    Use
    E.coli/bacillus spp. shuttle vector
    Expression
    Bacterial
    Promoter
    Pman
    Available Since
    Nov. 1, 2019
    Availability
    Academic Institutions and Nonprofits only
  3. pHM024Env

    Plasmid
    #137691
    Purpose
    Express HIV Env which is isolated a from IRB approved HIV infected patient samples from Singapore and cloned into expression vector (pcDNA 3.1)
    Depositor
    Insert
    HIV-1 Envelope Glycoprotein
    Expression
    Mammalian
    Available Since
    Feb. 28, 2020
    Availability
    Academic Institutions and Nonprofits only
  4. 3xFLAG-dCas9/pMXs-neo

    Plasmid
    #51260
    Purpose
    Expresses 3xFLAG-dCas9 in mammalian cells for enChIP analysis to purify specific genomic regions of interest.
    Depositor
    Insert
    3xFLAG-dCas9
    Use
    CRISPR and Retroviral
    Tags
    3xFLAG tag and NLS (nuclear localization signal)
    Expression
    Mammalian
    Mutation
    human codon-optimized, D10A + H840A
    Promoter
    LTR
    Available Since
    Feb. 20, 2014
    Availability
    Academic Institutions and Nonprofits only
  5. pSR646

    Plasmid
    #65215
    Purpose
    Baculovirus-mediated rAAV production: Expresses bicistronic AAV-2 rep gene and polycistronic AAV-6 cap gene in Sf9 insect cells
    Depositor
    Inserts
    modified AAV-2 rep78
    modified AAV-6 cap
    Use
    AAV
    Expression
    Insect
    Promoter
    P10 and polyhedrin
    Available Since
    May 19, 2015
    Availability
    Academic Institutions and Nonprofits only
  6. 3xFLAG-dCas9/pMXs-puro

    Plasmid
    #51240
    Purpose
    Expresses 3xFLAG-dCas9 in mammalian cells for enChIP analysis to purify specific genomic regions of interest.
    Depositor
    Insert
    3xFLAG-dCas9
    Use
    CRISPR and Retroviral
    Tags
    3xFLAG tag and NLS (nuclear localization signal)
    Expression
    Mammalian
    Mutation
    human codon-optimized, D10A + H840A
    Promoter
    LTR
    Available Since
    Aug. 14, 2014
    Availability
    Academic Institutions and Nonprofits only
  7. pX330_VCL sgRNA / hSpCas9

    Plasmid
    #172837
    Purpose
    Mammalian expression of a sgRNA targeting the intron 21 (last intron) of VCL (Zhong et al, eLife 2021) under the U6 promotor and hSpCas9 under the CAG promotor. This construct is based on pX330.
    Depositor
    Insert
    sgRNA targeting intron 21 of VCL under the U6 promotor and hSpCas9 under the CAG promotor
    Use
    CRISPR
    Expression
    Mammalian
    Available Since
    Aug. 24, 2021
    Availability
    Academic Institutions and Nonprofits only
  8. pSR660

    Plasmid
    #65216
    Purpose
    Baculovirus-mediated rAAV production: Expresses bicistronic AAV-2 rep gene and polycistronic AAV-8 cap gene in Sf9 insect cells
    Depositor
    Inserts
    modified AAV-2 rep78
    modified AAV-8 cap
    Use
    AAV
    Expression
    Insect
    Promoter
    P10 and polyhedrin
    Available Since
    June 10, 2015
    Availability
    Academic Institutions and Nonprofits only
  9. pAL149

    Plasmid
    #22275
    Purpose
    Expresses PhyB-mCherry with prenylation sequence from Kras, for red-light-induced recruitment of PIF to the plasma membrane
    Depositor
    Insert
    PhyB(1-908) (PHYB Mustard Weed)
    Use
    Synthetic Biology
    Tags
    mCherry-Kras4BCT (prenylation sequence)
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Oct. 21, 2009
    Availability
    Academic Institutions and Nonprofits only
  10. pX330_TOMM20 sgRNA / hSpCas9

    Plasmid
    #172836
    Purpose
    Mammalian expression of a sgRNA targeting the intron 4 (last intron) of TOMM20 (Zhong et al, eLife 2021) under the U6 promotor and hSpCas9 under the CAG promotor. This construct is based on pX330.
    Depositor
    Insert
    sgRNA targeting intron 4 of TOMM20 under the U6 promotor and hSpCas9 under the CAG promotor
    Use
    CRISPR
    Expression
    Mammalian
    Available Since
    Aug. 24, 2021
    Availability
    Academic Institutions and Nonprofits only
  11. pQCXIP-SHMY-PAUF

    Plasmid
    #182645
    Purpose
    Mammalian expression vector encoding a protein fusion composed of an N-terminal ER targeting sequence of hemagglutinin (HA), one tyrosine sulfation (TS) motif (Y), myc (M), His6 (H) and PAUF sequence
    Depositor
    Insert
    Pancreatic adenocarcinoma upregulated factor (PAUF)
    Use
    Retroviral
    Tags
    His6-tag, N-terminal ER targeting sequence of hem…
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    April 22, 2022
    Availability
    Academic Institutions and Nonprofits only
  12. pCRISPEY-GAL-Kan-ADE2

    Plasmid
    #232105
    Purpose
    Expresses galactose-inducible CRISPR guide RNA and repair template for creating a frameshift mutation in ADE2 gene of yeast.
    Depositor
    Insert
    ADE2 gRNA and repair template
    Use
    CRISPR
    Expression
    Yeast
    Mutation
    Repair template introduces C at nt 466 of ADE2 to…
    Promoter
    GAL7
    Available Since
    Feb. 21, 2025
    Availability
    Academic Institutions and Nonprofits only
Showing: 4921 - 4960 of 41926 results