We narrowed to 9,416 results for: Coli

1. pMBA333

   Plasmid

   #214745

   Purpose

   Medium copy thyA-infA plasmid containing gfp and no antibiotic resistance gene (ARG)

   Depositor

   Tae Seok Moon

   Article

   Amrofell et al Cell Rep Methods. 2023 Dec 18;3(12):1006

   Insert

   BBa_J23119-RiboJ-RBS(21992)-gfpmut3
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   May 13, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pLicC-MBP-APETx2

   Plasmid

   #72668

   Purpose

   Periplasmic expression of APETx2 in E.coli

   Depositor

   Glenn King

   Article

   Anangi et al Mar Drugs. 2012 Jul;10(7):1605-18. doi:

   Insert

   APETx2
   

   Use

   Tags

   His tag, MBP, MalE signal sequence for periplasmi…

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available Since

   Jan. 14, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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3. pET303-colE1TR

   Plasmid

   #155181

   Purpose

   Expresses colicin E1 residues 1-364

   Depositor

   Joanna Slusky

   Article

   Budiardjo et al Elife. 2022 Feb 24;11. pii: 73297. doi:

   Insert

   colicin E1 residues 1-364
   

   Use

   Tags

   C-terminal 6x Histidine tag

   Expression

   Bacterial

   Mutation

   truncation of colicin E1 including 1-364

   Promoter

   T7

   Available Since

   Aug. 16, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. aav-SYN-tdNfsB(F124W)-mCherry (eNTR)

   Plasmid

   #113760

   Purpose

   AAV-mediated expression of bacterial tdNfsB-mCherry (eNTR) under the SYN promoter

   Depositor

   Luke Lavis

   Article

   Gruber et al ACS Chem Biol. 2018 Aug 29. doi: 10.1021

   Insert

   tdNfsB(F124W)-mCherry
   

   Use

   AAV

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   F124W

   Promoter

   Synapsin

   Available Since

   Oct. 11, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. pAC-4CL1

   Plasmid

   #35947

   Depositor

   Claudia Schmidt-dannert

   Article

   Watts et al BMC Biotechnol. 2006 Mar 21;6:22.

   Insert

   4-coumaroyl CoA ligase 1 (4CL1 Mustard Weed)
   

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   pLac-mod

   Available Since

   Sept. 5, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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6. pPLtetO-ClpX

   Plasmid

   #89657

   Purpose

   Plasmid for titrating synthesis of ClpX with doxycycline

   Depositor

   Uwe Sauer

   Article

   Sekar et al Mol Syst Biol. 2018 Nov 5;14(11):e8623.

   Insert

   clpX
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   May 8, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. PET-3a-chpBK

   Plasmid

   #155201

   Purpose

   To express recombinant ChpBK protein

   Depositor

   Guanzheng Luo

   Article

   Zhang et al Sci Adv. 2019 Jul 3;5(7):eaax0250. doi:

   Inserts

   chpS-chpBK-6×His-Tag
   GyrA-CBD-10xHis-Tag
   

   Use

   Tags

   10xHis and 6xHis

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   Dec. 17, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. pDEINO4

   Plasmid

   #179488

   Purpose

   Multi-host shuttle plasmid for E. coli, D. radiodurans, and S. cerevisiae; contains an oriT for transfer via conjugation

   Depositor

   Bogumil Karas

   Article

   Brumwell et al ACS Synth Biol. 2022 Mar 18;11(3):1068-1

   Insert

   aadA1
   

   Use

   Synthetic Biology; Bacterial and yeast cloning

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   May 10, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pNAS1B split mCherry N-link/link-C

   Plasmid

   #61965

   Purpose

   Negative control duet plasmid for split mCherry assembly assay (i.e., neither half of mCherry is fused to another peptide or protein)

   Depositor

   Lynne Regan

   Article

   Sawyer et al ACS Chem Biol. 2014 Nov 21;9(11):2502-7.

   Inserts

   N-mCherry-link
   link-C-mCherry
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   PBAD and PLtetO

   Available Since

   Feb. 13, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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10. pDisplay FLIPE-600n

    Plasmid

    #13545

    Depositor

    Wolf Frommer

    Article

    Okumoto et al Proc Natl Acad Sci U S A. 2005 Jun 14. 1

    Insert

    FLIP-E WT
    

    Use

    Tags

    ECFP and Venus

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Dec. 15, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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11. pcDNA3.1 FLIPE-1m

    Plasmid

    #13544

    Depositor

    Wolf Frommer

    Article

    Okumoto et al Proc Natl Acad Sci U S A. 2005 Jun 14. 1

    Insert

    FLIP-E A207W
    

    Use

    Tags

    ECFP and Venus

    Expression

    Mammalian

    Mutation

    A207W in binding protein

    Promoter

    Available Since

    Dec. 15, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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12. pDR111_Bgal NLS

    Plasmid

    #188396

    Purpose

    Expresses beta-galactosidase via Phyper-spank which is optimized for Bacillus subtilis. There is a secretion peptide (PhoD) and nuclear localization signal (SV40) that is fused to beta-galactosidase.

    Depositor

    Christopher Contag

    Article

    Madsen et al Commun Biol. 2022 Aug 30;5(1):888. doi:

    Insert

    lacZ
    

    Use

    Tags

    PhoD secretion peptide and SV40 NLS

    Expression

    Bacterial

    Mutation

    Promoter

    Phyper spank

    Available Since

    Sept. 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. pRSFDuet-1_DARPinOFF7

    Plasmid

    #182396

    Purpose

    Bacterial expression of DARPinOFF7

    Depositor

    Dan Mulvihill

    Article

    Eastwood et al Cell Rep Methods. 2023 Feb 2;3(2):100396

    Insert

    DARPinOFF7
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    Feb. 3, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. pBEL1203

    Plasmid

    #196024

    Purpose

    Expression/purification of 6xHis-TEV-MlaC

    Depositor

    Damian Ekiert, Gira Bhabha

    Article

    MacRae et al J Biol Chem. 2023 Apr 24:104744. doi: 10

    Insert

    MlaC
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    May 2, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. pTS2365-Tier1-PhCMV-TetR

    Plasmid

    #169566

    Purpose

    Tier-1 vector encoding PhCMV-driven tetracycline dependent regulator protein TetR (PhCMV-TetR-pA).

    Depositor

    Martin Fussenegger

    Article

    Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

    Insert

    PCMV-driven tetracycline repressor protein class B from transposon Tn10
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    PhCMV

    Available Since

    June 23, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. pAJT248

    Plasmid

    #202289

    Purpose

    RPU integration plasmid (LPattB2)

    Depositor

    Christopher Voigt

    Article

    Triassi et al Cell Syst. 2023 Jun 21;14(6):512-524.e12

    Insert

    J23101-RiboJ00-B0034-YFP
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Sept. 18, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. pcDNA5_NLS-Halo-eGFP-BioID

    Plasmid

    #218448

    Purpose

    expresses the NLS-Halo-eGFP-BioID fusion protein via stable Flp-In integration

    Depositor

    Thorsten Stafforst

    Article

    Hanswillemenke et al Nat Chem Biol. 2024 Jan 17. doi: 10.1038

    Insert

    NLS-Halo-eGFP-BirA
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    June 10, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. pcDNA3-LplA(W37I) Alternative coumarin PRIME ligase

    Plasmid

    #25846

    Depositor

    Alice Ting

    Article

    Uttamapinant et al Proc Natl Acad Sci U S A. 2010 Jun 15. 1

    Insert

    LplA-W37I
    

    Use

    Tags

    Flag and His

    Expression

    Mammalian

    Mutation

    W37I

    Promoter

    Available Since

    July 27, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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19. pRSET FLIPE intramol-1u

    Plasmid

    #13549

    Depositor

    Wolf Frommer

    Article

    Deuschle et al Protein Sci. 2005 Sep . 14(9):2304-14.

    Insert

    FLIP-E intramol WT (gltI E. coli)
    

    Use

    Tags

    EYFP

    Expression

    Bacterial

    Mutation

    ECFP insert in binding protein

    Promoter

    Available Since

    Dec. 15, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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20. pLV-EF1a-PKAsub-mCherry-MinD-IRES-Puro

    Plasmid

    #213118

    Purpose

    Expression of one half of a PKAsub-mCherry-MinD reporter circuit in mammalian cells

    Depositor

    Scott Coyle

    Article

    Rajasekaran et al Cell. 2023 Dec 27:S0092-8674(23)01339-9.

    Insert

    PKAsub-mCherry-MinD (minD Escherichia coli)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available Since

    Jan. 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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21. pCB11

    Plasmid

    #201111

    Purpose

    pSR58.6 (Addgene plasmid #63176) modified to include the tetA gene upstream of sfGFP.

    Depositor

    Mary Dunlop

    Article

    Lugagne et al Nat Commun. 2024 Mar 8;15(1):2148. doi:

    Inserts

    CcaR
    tetA(C)
    sfGFP
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    C-to-T transition at pos. no. 1049 of tetA(C) lea…

    Promoter

    J23100 and PcpcG2-172

    Available Since

    March 11, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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22. cre_CD

    Plasmid

    #66030

    Purpose

    MoClo Basic Part: CDS - cre recombinase (recombines DNA at loxP sites) [C:cre:D]

    Depositor

    Douglas Densmore

    Article

    Iverson et al ACS Synth Biol. 2016 Jan 15;5(1):99-103.

    Insert

    Recombinase
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Jan. 21, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pFLIPglu-2uDelta13

    Plasmid

    #12990

    Depositor

    Wolf Frommer

    Article

    Deuschle et al Plant Cell. 2006 Sep . 18(9):2314-25.

    Insert

    FLIPglu F16F D152A Delta13
    

    Use

    Plant nanosensor

    Tags

    ECFP and EYFP

    Expression

    Plant

    Mutation

    D152A mutation in binding protein

    Promoter

    Available Since

    Jan. 5, 2007

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pLEX-uORF-HA-birA*-STOP-IRES-Puro

    Plasmid

    #120558

    Purpose

    Expresses control HA-birA* in mammalian cells & for virus production

    Depositor

    Paul Khavari

    Article

    Kovalski et al Mol Cell. 2019 Jan 4. pii: S1097-2765(18

    Insert

    birA
    

    Use

    Lentiviral

    Tags

    HA

    Expression

    Mammalian

    Mutation

    R118G

    Promoter

    CMV

    Available Since

    Jan. 30, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. plYJP067-Ptet

    Plasmid

    #202299

    Purpose

    PTet-YFP plasmid for LPattB2 integration

    Depositor

    Christopher Voigt

    Article

    Triassi et al Cell Syst. 2023 Jun 21;14(6):512-524.e12

    Insert

    Ptet-YFP
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Sept. 18, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pcDNA3.1 FLIPE-100u

    Plasmid

    #13543

    Depositor

    Wolf Frommer

    Article

    Okumoto et al Proc Natl Acad Sci U S A. 2005 Jun 14. 1

    Insert

    FLIP-E S118W
    

    Use

    Tags

    ECFP and Venus

    Expression

    Mammalian

    Mutation

    S118W in binding protein

    Promoter

    Available Since

    Dec. 15, 2006

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pFLIPglu-600uDelta13

    Plasmid

    #12991

    Depositor

    Wolf Frommer

    Article

    Deuschle et al Plant Cell. 2006 Sep . 18(9):2314-25.

    Insert

    FLIPglu F16A Delta13
    

    Use

    Plant nanosensor

    Tags

    ECFP and EYFP

    Expression

    Plant

    Mutation

    F16A in binding protein

    Promoter

    Available Since

    Jan. 5, 2007

    Availability

    Academic Institutions and Nonprofits only

28. pMBA323

    Plasmid

    #214751

    Purpose

    To create delta(glmS,nadE) working strains and maintaining their viability

    Depositor

    Tae Seok Moon

    Article

    Amrofell et al Cell Rep Methods. 2023 Dec 18;3(12):1006

    Insert

    BBa_J23105-cas9 + pBAD-λ Red genes + ptet-gRNA (pBR322ori)
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    April 19, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pAJT430

    Plasmid

    #202291

    Purpose

    RPU integration plasmid (LPattB7)

    Depositor

    Christopher Voigt

    Article

    Triassi et al Cell Syst. 2023 Jun 21;14(6):512-524.e12

    Insert

    J23101-RiboJ00-B0034-YFP
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Sept. 18, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pET22b-BirA-CPDSalI

    Plasmid

    #38258

    Depositor

    Aimee Shen, Matthew Bogyo

    Article

    Shen et al PLoS One. 2009 Dec 2;4(12):e8119.

    Insert

    birA (birA E. coli)
    

    Use

    Tags

    6xHis and Vibrio cholerae MARTX toxin cysteine pr…

    Expression

    Bacterial

    Mutation

    cloned from pGEX4T1-BirA

    Promoter

    T7

    Available Since

    July 20, 2012

    Availability

    Academic Institutions and Nonprofits only