We narrowed to 8,446 results for: gnal

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2xP53_RE::NLuc

Plasmid

#124533

Purpose

p53 NLuc luciferase reporter

Depositor

Koen Venken

Article

Sarrion-Perdigones et al Curr Protoc Mol Biol. 2020 Jun;131(1):e1

Insert

P53 NLuc reporter

Use

Luciferase and Synthetic Biology

Expression

Mammalian

Available Since

June 17, 2020

Availability

Academic Institutions and Nonprofits only

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pTRIPZ-EGFP:LIMD1

Plasmid

#108230

Purpose

Lentiviral vector for Tet-inducible LIMD1 expression

Depositor

Kenneth Irvine

Article

Ibar et al J Cell Sci. 2018 Feb 13. pii: jcs.214700

Insert

LIMD1 (LIMD1 Human)

Use

Lentiviral

Tags

EGFP

Expression

Mammalian

Promoter

Tet/ON Minimal CMV promoter

Available Since

July 25, 2018

Availability

Academic Institutions and Nonprofits only

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TFAM-SNAPf

Plasmid

#187909

Purpose

Mammalian cell expression of SNAPf-labeled TFAM

Depositor

Erika Holzbaur

Article

Harding et al Mol Cell. 2023 Sep 7;83(17):3188-3204.e7

Insert

TFAM (TFAM Human)

Tags

SNAPf

Expression

Mammalian

Promoter

CMV

Available Since

April 2, 2024

Availability

Academic Institutions and Nonprofits only

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EGFP-Ubiquitin

Plasmid

#187910

Purpose

Mammalian cell expression of EGFP-tagged Ubiquitin

Depositor

Erika Holzbaur

Article

Harding et al Mol Cell. 2023 Sep 7;83(17):3188-3204.e7

Insert

UBB (UBB Human)

Tags

EGFP

Expression

Mammalian

Promoter

CMV

Available Since

April 2, 2024

Availability

Academic Institutions and Nonprofits only

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pLVX-NPC1(WT)-FLAG

Plasmid

#164972

Purpose

Lentiviral vector for NPC1 expression in mammalian cells

Depositor

Roberto Zoncu

Article

Davis et al Dev Cell. 2020 Dec 7. pii: S1534-5807(20

Insert

NPC1 (NPC1 Human)

Use

Lentiviral

Tags

FLAG

Expression

Mammalian

Promoter

EF1a

Available Since

Jan. 4, 2022

Availability

Academic Institutions and Nonprofits only

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pEYFP-N1-A2BR

Plasmid

#37202

Depositor

Robert Tarran

Article

Watson et al FASEB J. 2011 Sep;25(9):2996-3003. Epub

Insert

A2BR (ADORA2B Human)

Tags

EYFP

Expression

Mammalian

Promoter

CMV

Available Since

July 16, 2012

Availability

Academic Institutions and Nonprofits only

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pcDNA3-MEK5DD-HA

Plasmid

#65247

Purpose

Expression of HA tagged dominant active MEK5 in mammalian cells

Depositor

Axel Ullrich

Article

Buschbeck et al J Biol Chem. 2002 Aug 16;277(33):29503-9

Insert

MEK5DD (MAP2K5 Human)

Tags

HA

Expression

Mammalian

Mutation

changed Ser311 to Asp, Ser315 to Asp

Promoter

CMV

Available Since

Nov. 7, 2016

Availability

Academic Institutions and Nonprofits only

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HA-mRIN2

Plasmid

#108940

Purpose

Mammalian expression of mRIN2

Depositor

Guido Serini

Article

Sandri et al Cell Res. 2012 Oct;22(10):1479-501. doi:

Insert

Ras and Rab interactor 2 (Rin2 Mouse)

Tags

HA

Expression

Mammalian

Promoter

CMV

Available Since

June 25, 2018

Availability

Academic Institutions and Nonprofits only

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pLenti_Puro_DEST mouse_p65promoter_mouse_p65-Clover

Plasmid

#124549

Purpose

Lentiviral vector to express p65-Clover under p65 promoter (With puromycin resistance)

Depositor

Markus Covert

Article

Lane et al Cell Syst. 2017 Apr 26;4(4):458-469.e5.

Insert

p65 promoter- p65 CDS-Clover

Use

Lentiviral

Tags

Clover

Expression

Mammalian

Available Since

May 15, 2019

Availability

Academic Institutions and Nonprofits only

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AAV-FLEX-FLIM-AKAR

Plasmid

#60445

Purpose

Expresses the Fluorescence Lifetime Imaging Microscopy (FLIM)-compatible PKA activity sensor FLIM-AKAR in a Cre dependent manner in an AAV backbone

Depositor

Bernardo Sabatini

Article

Chen et al Front Pharmacol. 2014 Apr 2;5:56. doi: 1

Insert

FLIM-AKAR

Use

AAV and Cre/Lox

Expression

Mammalian

Promoter

CAG

Available Since

Oct. 22, 2014

Availability

Academic Institutions and Nonprofits only

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Parkin

Plasmid

#187897

Purpose

Mammalian cell expression of untagged Parkin

Depositor

Erika Holzbaur

Article

Harding et al Mol Cell. 2023 Sep 7;83(17):3188-3204.e7

Insert

PRKN (PRKN Human)

Tags

None

Expression

Mammalian

Mutation

None

Promoter

CMV

Available Since

April 2, 2024

Availability

Academic Institutions and Nonprofits only

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pRK5-HA-FNIP1

Plasmid

#72293

Purpose

overexpression

Depositor

David Sabatini

Article

Tsun et al Mol Cell. 2013 Nov 21;52(4):495-505. doi

Insert

FNIP1 (FNIP1 Human)

Tags

HA

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 2, 2016

Availability

Academic Institutions and Nonprofits only
pGEX6P-2-human RNF146 full length

Plasmid

#132610

Purpose

expresses human RNF146 full length in E. coli

Depositor

Wenqing Xu

Article

DaRosa et al Nature. 2015 Jan 8;517(7533):223-6. doi:

Insert

RNF146 (RNF146 Human)

Expression

Bacterial

Available Since

Oct. 17, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-HA-mSTING

Plasmid

#214157

Purpose

Transient expression of STING

Depositor

Nan Yan

Article

Chu et al Nature. 2021 Aug;596(7873):570-575. doi:

Insert

STING (Sting1 Mouse)

Tags

HA

Expression

Mammalian

Promoter

CMV

Available Since

March 5, 2024

Availability

Academic Institutions and Nonprofits only

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pSG5-HA-p300

Plasmid

#89094

Purpose

mammalian expression of full-length human p300 in pSG5

Depositor

Elizabeth Wilson

Article

Askew et al J Biol Chem. 2010 Jul 9;285(28):21824-36

Insert

p300 (EP300 Human)

Tags

HA

Expression

Mammalian

Promoter

SV40

Available Since

April 21, 2017

Availability

Academic Institutions and Nonprofits only

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pRK5-p18-FLAG

Plasmid

#42331

Depositor

David Sabatini

Article

Bar-Peled et al Cell. 2012 Sep 14;150(6):1196-208. doi:

Insert

p18 (LAMTOR1 Human)

Tags

Flag

Expression

Mammalian

Promoter

CMV, SP6

Available Since

March 6, 2013

Availability

Academic Institutions and Nonprofits only

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pcDNA5FRT/TO-UBXD7-Strep-HA

Plasmid

#113479

Purpose

Expression of human UBXD7 with C-terminal strep-HA tag

Depositor

Hemmo Meyer

Article

Hulsmann et al Mol Cell Proteomics. 2018 Mar 29. pii: R

Insert

UBXD7 (UBXN7 Human)

Tags

2xstrep-HA

Expression

Mammalian

Mutation

A834C silent mutation, wildtype without stop codon

Promoter

CMV

Available Since

Aug. 15, 2018

Availability

Academic Institutions and Nonprofits only

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pLenti6/V5/DEST-VPS35

Plasmid

#21691

Depositor

Lynda Chin

Article

Scott et al Nature. 2009 Jun 25. 459(7250):1085-90.

Insert

Vacuolar Protein Sorting 35 homolog (VPS35 Human)

Use

Lentiviral; Invitrogen gateway destination vector

Tags

V5

Expression

Mammalian

Available Since

July 28, 2009

Availability

Academic Institutions and Nonprofits only

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pDL025_HA-rb_IgG_HC_15F11

Plasmid

#193622

Purpose

HA-tag heavy chain hypervariable regions grafted into 15F11 scaffold (Zhao et al., 2019) + rabbit heavy chain constant regions + exogenous N-term signal peptide. For co-expression with pDL026.

Depositor

Jennifer DeLuca

Article

DeLuca et al Elife. 2021 Dec 31;10. pii: 72093. doi:

Insert

Anti_HA_15F11 rabbit heavy chain

Tags

signal peptide

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 21, 2022

Availability

Industry, Academic Institutions, and Nonprofits

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pDL026_HA-rb_IgG_LC_15F11

Plasmid

#193623

Purpose

HA-tag light chain hypervariable regions grafted into 15F11 scaffold (Zhao et al., 2019) + rabbit light chain constant regions + exogenous N-term signal peptide. For co-expression with pDL025.

Depositor

Jennifer DeLuca

Article

DeLuca et al Elife. 2021 Dec 31;10. pii: 72093. doi:

Insert

Anti-HA_15F11 rabbit light chain

Tags

signal peptide

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 21, 2022

Availability

Industry, Academic Institutions, and Nonprofits

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pGEX6P1-mCherry-anti-mouse-IgG(kappa)-nanobody

Plasmid

#138130

Purpose

Expresses GST-tagged mCherry-anti-mouse-IgG(kappa)-nanobody in E. coli cells.

Depositor

Kazuhisa Nakayama

Article

Katoh et al Mol Biol Cell. 2020 Jul 29:mbcE20040250.

Insert

mCherry-anti-mouse-IgG(kappa)-nanobody

Tags

GST

Expression

Bacterial

Available Since

July 30, 2020

Availability

Academic Institutions and Nonprofits only

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pGEX6P1-EGFP-anti-mouse-IgG(kappa)-nanobody

Plasmid

#138132

Purpose

Expresses GST-tagged EGFP-anti-mouse-IgG(kappa)-nanobody in E. coli cells.

Depositor

Kazuhisa Nakayama

Article

Katoh et al Mol Biol Cell. 2020 Jul 29:mbcE20040250.

Insert

EGFP-anti-mouse-IgG(kappa)-nanobody

Tags

GST

Expression

Bacterial

Available Since

July 30, 2020

Availability

Academic Institutions and Nonprofits only

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pEM705 Gs

Plasmid

#109350

Purpose

Wildtype Gαs

Depositor

Robert Lucas

Article

Ballister et al BMC Biol. 2018 Jan 16;16(1):10. doi: 10.

Insert

GαS (GNAS Human)

Tags

None

Expression

Mammalian

Promoter

CAG

Available Since

May 14, 2018

Availability

Academic Institutions and Nonprofits only

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pLVX-NPC1(P691S)-FLAG

Plasmid

#164973

Purpose

Lentiviral vector for NPC1 expression in mammalian cells

Depositor

Roberto Zoncu

Article

Davis et al Dev Cell. 2020 Dec 7. pii: S1534-5807(20

Insert

NPC1 (NPC1 Human)

Use

Lentiviral

Tags

FLAG

Expression

Mammalian

Mutation

P691S

Promoter

EF1a

Available Since

March 19, 2021

Availability

Academic Institutions and Nonprofits only

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pCMV BiP-Myc-KDEL-wt

Plasmid

#27164

Purpose

Mammalian expression of human Bip with Myc tag near the C-terminus, retaining the C-terminal KDEL ER localization sequence

Depositor

Ron Prywes

Article

Shen et al Dev Cell. 2002 Jul . 3(1):99-111.

Insert

BiP (HSPA5 Human)

Tags

KDEL and Myc

Expression

Mammalian

Available Since

Jan. 25, 2011

Availability

Academic Institutions and Nonprofits only

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HA-Vps34

Plasmid

#86749

Purpose

N-terminal HA-tagged protein expression in mammalian cells

Depositor

Do-Hyung Kim

Article

Park et al Autophagy. 2016;12(3):547-64. doi: 10.10

Insert

human Vps34 (PIK3C3 Human)

Tags

HA

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 26, 2017

Availability

Academic Institutions and Nonprofits only

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pCI-neo beta catenin WT

Plasmid

#16518

Depositor

Bert Vogelstein

Article

Morin et al Science. 1997 Mar 21. 275(5307):1787-90.

Insert

beta catenin (CTNNB1 Human)

Expression

Mammalian

Available Since

April 7, 2008

Availability

Academic Institutions and Nonprofits only

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opto-Cdc42

Plasmid

#174510

Purpose

Expresses opto-Cdc42 (mCherry-GGGSx2-Cdc42-GGGSx2-BcLOV4-GGGS-3xFLAG) in a pcDNA3.1 backbone.

Depositor

Brian Chow

Article

Berlew et al ACS Synth Biol. 2022 Jan 21;11(1):515-52

Insert

opto-Cdc42-mCherry

Tags

3xFLAG and mCherry

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 7, 2023

Availability

Academic Institutions and Nonprofits only

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HA-MBP-TEVpCS-ADGRC3 CTF-FLAG

Plasmid

#217686

Purpose

Mammalian expression of Adhesion G Protein-Coupled Receptor transmembrane domains

Depositor

Stephen Blacklow

Article

Dates et al bioRxiv 2022.07.14.500097

Insert

CELSR3 (CELSR3 Human)

Tags

FLAG and HA

Expression

Mammalian

Mutation

Deletion of residues 1-2516

Available Since

April 18, 2024

Availability

Academic Institutions and Nonprofits only

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pSOMA-NLS

Plasmid

#118937

Purpose

Used to express the SOMA FRET sensor with a nuclear localization signal in Arabidopsis thaliana

Depositor

Patrick Krysan

Article

Zaman et al Plant J. 2018 Nov 16. doi: 10.1111/tpj.1

Insert

SOMA Sensor for Arabidopsis MAPK activity with Nuclear Localization Signal

Expression

Plant

Available Since

Feb. 20, 2019

Availability

Academic Institutions and Nonprofits only

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