We narrowed to 15,050 results for: pET

1. pET-45b-mEGFP-HMGB1-K184Rfs

   Plasmid

   #194544

   Purpose

   Bacterial Expression of 6xHis mEGFP-HMGB1 Mutant (K184Rfs*44) Fusion Protein

   Depositor

   Denes Hnisz

   Article

   Mensah et al Nature. 2023 Feb;614(7948):564-571. doi:

   Insert

   HMGB1 (HMGB1 Human)
   

   Tags

   mEGFP

   Expression

   Bacterial

   Mutation

   K184Rfs*44

   Available Since

   Feb. 23, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pEW4: pET-32a Dot1L(2-386)

   Plasmid

   #124099

   Purpose

   Dot1L(2-386) expression plasmid

   Depositor

   Cynthia Wolberger

   Article

   Worden et al Cell. 2019 Feb 8. pii: S0092-8674(19)301

   Insert

   Dot1L(2-386) (DOT1L Human)
   

   Tags

   Trx-His-TEV

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   April 2, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. pET-28a+GFP-CAMSAP2 CKK

   Plasmid

   #59032

   Purpose

   expresses CKK domain of CAMSAP2 in E. coli

   Depositor

   Ron Vale

   Article

   Hendershott et al Proc Natl Acad Sci U S A. 2014 Apr 22;11

   Insert

   CAMSAP2 CKK domain (CAMSAP2 Human)
   

   Tags

   His, Strep, TEV, and sfGFP

   Expression

   Bacterial

   Available Since

   Aug. 27, 2014

   Availability

   Academic Institutions and Nonprofits only

4. pET30a-SARS-CoV2-ORF3b (S23Q)

   Plasmid

   #165088

   Purpose

   protein expression in E. coli

   Depositor

   Rick Tarleton

   Article

   Shen et al medRxiv [Preprint]. 2021 Jan 27:2021.01.

   Insert

   Severe acute respiratory syndrome coronavirus 2 ORF3b (S23Q)
   

   Expression

   Bacterial

   Mutation

   Stop codon to glutamine at 23rd amino acid

   Available Since

   Feb. 10, 2021

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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5. pET-FLAG-SpCas9-HF1-plus

   Plasmid

   #126775

   Purpose

   Expression of increased fidelity SpCas9-HF1-plus in bacterial cells. Cleaves both 20nt and 5'-extended 21nt spacer containing sgRNAs with close to the same fidelity as SpCas9-HF1.

   Depositor

   Ervin Welker

   Article

   Kulcsar et al Nat Commun. 2020 Mar 6;11(1):1223. doi:

   Insert

   SpCas9-HF1-plus
   

   Use

   CRISPR

   Tags

   3xFLAG, 6xHis, MBP, and NLS

   Expression

   Bacterial

   Mutation

   N497A; Q695A; Q926A; amino acids 1005-1013 replac…

   Promoter

   T7

   Available Since

   March 6, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. pET28-His8-aCat(660-906)

   Plasmid

   #203333

   Purpose

   Bacterial expression of alpha-catenin F-actin binding domain

   Depositor

   Tina Izard

   Article

   Rangarajan et al J Biol Chem. 2023 Feb;299(2):102817. doi

   Insert

   CTNNA1 (CTNNA1 Human)
   

   Tags

   8xHis with precision protease cleavage site

   Expression

   Bacterial

   Mutation

   aa 660-906 only

   Available Since

   Aug. 21, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pET21c-His-Xa-Gluc-Xa-C9D-TGinsert

   Plasmid

   #124660

   Purpose

   reporter protein which emits bright blue fluorescence

   Depositor

   Yutaka Kuroda

   Article

   Wu et al Sci Rep. 2020 Nov 18;10(1):20069. doi: 1

   Insert

   Gaussia luciferase-C9D-TGinsert
   

   Tags

   IEGRTMGDDDGDDDGDDD（FactorXa,TMG,C9D-Tag） and MHHH…

   Expression

   Bacterial

   Mutation

   changed glutamic acid 100 to alanine, and glycine…

   Promoter

   T7 promoter

   Available Since

   Dec. 1, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. pET-Duet1-6xHis-TEV-LC3BGlydelta5C

   Plasmid

   #190237

   Purpose

   Plasmid for the expression and purification of 6xHis-TEV-LC3BGlydelta5C. Internal reference: SMC893.

   Depositor

   Sascha Martens

   Article

   Fracchiolla et al J Cell Biol. 2020 Jul 6;219(7). pii: 151

   Insert

   MAP1LC3B (MAP1LC3B Human)
   

   Tags

   6xHis-TEV

   Expression

   Bacterial

   Available Since

   Sept. 29, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. HOXA11 (murine) HIS-tag pET

   Plasmid

   #8517

   Depositor

   Corey Largman

   Article

   Shen et al Mol Cell Biol 1997 Nov;17(11):6448-58.

   Insert

   HOXA11 (Hoxa11 Mouse)
   

   Tags

   T7

   Expression

   Bacterial

   Mutation

   makes protein by Western

   Available Since

   May 30, 2006

   Availability

   Academic Institutions and Nonprofits only

10. HOXA2 (murine) HIS-tag pET

    Plasmid

    #8557

    Depositor

    Corey Largman

    Insert

    HOXA2 (Hoxa2 Mouse)
    

    Tags

    His and T7

    Expression

    Bacterial

    Mutation

    contains 5 additional amino acids from 5' un…

    Available Since

    May 30, 2006

    Availability

    Academic Institutions and Nonprofits only

11. HOXB2 (human) HIS-tag pET

    Plasmid

    #8522

    Depositor

    Corey Largman

    Article

    Shen et al Nucleic Acids Res 1996 Mar 1;24(5):898-9

    Insert

    HOXB2 (HOXB2 Human)
    

    Tags

    His and T7

    Expression

    Bacterial

    Mutation

    Starts at AA #2; makes protein by Western

    Available Since

    May 30, 2006

    Availability

    Academic Institutions and Nonprofits only

12. pET-28a+GFP-CAMSAP1 CKK

    Plasmid

    #59031

    Purpose

    expresses CKK domain of CAMSAP1 in E. coli

    Depositor

    Ron Vale

    Article

    Hendershott et al Proc Natl Acad Sci U S A. 2014 Apr 22;11

    Insert

    CAMSAP1 CKK domain (CAMSAP1 Human)
    

    Tags

    His, Strep, TEV, and sfGFP

    Expression

    Bacterial

    Available Since

    Aug. 27, 2014

    Availability

    Academic Institutions and Nonprofits only

13. pET28a-ybbr-HIS-ddFLN4-K10

    Plasmid

    #101718

    Purpose

    K10 peptide with ddFLN4 fingerprint domain and N-terminal ybbr tag for covalent immobilization - resulting in high forces

    Depositor

    Hermann Gaub

    Article

    Milles et al Science. 2018 Mar 30;359(6383):1527-1533

    Insert

    ddFLN4 fingerprint, K10 peptide (KRT10 Human, Dictyostelium discoideum)
    

    Tags

    HIS and ybbr

    Expression

    Bacterial

    Mutation

    C18S in ddFLN4

    Promoter

    T7

    Available Since

    March 29, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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14. pET-dSpCas9-HF1-VP64-6xHis

    Plasmid

    #92118

    Purpose

    Expression of dead/inactive increased fidelity SpCas9-HF1-VP64-6xHis in bacterial cells

    Depositor

    Ervin Welker

    Article

    Kulcsar et al Genome Biol. 2017 Oct 6;18(1):190. doi:

    Insert

    dead/inactive SpCas9-HF1-NLS-3xFLAG-VP64
    

    Use

    CRISPR

    Tags

    3xFLAG, 6xHis, NLS, and VP64

    Expression

    Bacterial

    Mutation

    D10A, N497A, R661A, Q695A, H840A, Q926A

    Promoter

    T7

    Available Since

    Oct. 11, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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15. Arabidopsis thaliana Atm3 d80 pET21a+

    Plasmid

    #173045

    Purpose

    Expresses AtAtm3 in E.coli

    Depositor

    Douglas Rees

    Article

    Fan et al Elife. 2022 Mar 25;11. pii: 76140. doi:

    Insert

    Arabidopsis thaliana Atm3 (ABCB25 Mustard Weed)
    

    Tags

    6xHis tag

    Expression

    Bacterial

    Mutation

    with N-terminal 80 amino acids deletion

    Available Since

    Oct. 13, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. pET23A-Fucosidase-E1_10125-Ruminococcus_gnavus-His

    Plasmid

    #207665

    Purpose

    Expresses fucosidase E1_10125 from Ruminococcus gnavus in BL21 cells, purified using His-tag

    Depositor

    Robert de Vries

    Article

    Spruit et al bioRxiv 2023.12.15.571923

    Insert

    Fucosidase E1_10125
    

    Tags

    His-tag

    Expression

    Bacterial

    Mutation

    amino acids 34-584 were used

    Promoter

    T7 promoter

    Available Since

    Dec. 18, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. pET11-His6-Pif6-sfGFP-ssrA

    Plasmid

    #140891

    Purpose

    Expression of PIF-sfGFP-ssrA in E. coli

    Depositor

    Neal Devaraj

    Article

    Bhattacharya et al Proc Natl Acad Sci U S A. 2020 Aug 4;117

    Insert

    PIF-sfGFP-ssrA
    

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Sept. 29, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. pET-28a(+)-His(6)-TEV-AtPCO4-1(wt)

    Plasmid

    #191801

    Purpose

    Full length wt AtPCO4 with Nt-His tag

    Depositor

    Emily Flashman

    Article

    White et al J Biol Chem. 2018 Jul 27;293(30):11786-1

    Insert

    AtPCO4-1
    

    Tags

    6xHis, TEV cleavage site

    Expression

    Bacterial

    Available Since

    Dec. 15, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. pET-28a-His6-SUMO-VAMP2

    Plasmid

    #135551

    Purpose

    Express mouse VAMP2 in E. coli BL21, purified via HisPur Ni-NTA Resin

    Depositor

    Jingshi Shen

    Article

    Yu et al Methods Mol Biol. 2019;1860:237-249. doi

    Insert

    VAMP2
    

    Tags

    6xHis tag, thrombin recognition and cleavage site…

    Expression

    Bacterial

    Mutation

    No ATG start codon

    Promoter

    T7 promoter

    Available Since

    Feb. 11, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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20. pET-GST-TNRC6A-(935-984)

    Plasmid

    #42050

    Depositor

    Kumiko Ui-Tei

    Article

    Nishi et al RNA. 2013 Jan;19(1):17-35. doi: 10.1261/

    Insert

    TNRC6A (TNRC6A Human)
    

    Tags

    GST and His

    Expression

    Bacterial

    Mutation

    contain aa935-984

    Available Since

    March 6, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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21. pET51b(+)-NADPH-LUCID-1 (C50 5.1 nM)

    Plasmid

    #117908

    Purpose

    Plasmid encoding the bacterial expression of the BRET semi-synthetic biosensor measuring the NADPH concentration with a C50 of 5.1 nM

    Depositor

    Kai Johnsson

    Article

    Yu et al Science. 2018 Sep 14;361(6407):1122-1126

    Insert

    SNAP_Pro30_[cpNLuc23_24]eDHFR
    

    Tags

    His-tag and Strep-tag

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Oct. 25, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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22. HOXA1 (murine) HIS-tag pET

    Plasmid

    #8556

    Depositor

    Corey Largman

    Insert

    HOXA1 (Hoxa1 Mouse)
    

    Tags

    His and T7

    Expression

    Bacterial

    Mutation

    clone contains 130 AA including the homeodomain, …

    Available Since

    July 5, 2006

    Availability

    Academic Institutions and Nonprofits only

23. pF708-pET28a-Hs-delta38-METTL20-NHis wt

    Plasmid

    #85109

    Purpose

    expression of recombinant human mature (delta38) METTL20 wt with N-terminal 6xHis-tag

    Depositor

    Pal Falnes

    Article

    Malecki et al J Biol Chem. 2015 Jan 2;290(1):423-34. d

    Insert

    human mature (delta38) METTL20 (METTL20 Human)
    

    Tags

    6xHis

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Nov. 12, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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24. pF711-pET28a-Hs-delta25-MCAD-NHis wt

    Plasmid

    #85112

    Purpose

    expression of recombinant human mature (delta25) MCAD wt with N-terminal 6xHis-tag

    Depositor

    Pal Falnes

    Article

    Malecki et al J Biol Chem. 2015 Jan 2;290(1):423-34. d

    Insert

    human mature (delta25) MCAD (ACADM Human)
    

    Tags

    6xHis

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Nov. 12, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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25. pET21b-LOV-ipaA L514K L531E

    Plasmid

    #40253

    Depositor

    Brian Kuhlman

    Article

    Lungu et al Chem Biol. 2012 Apr 20;19(4):507-17.

    Insert

    LOV-ipaA L514K L531E
    

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    L514K; L531E; Jα helix region hybridized (AsLOV2 …

    Promoter

    T7

    Available Since

    Sept. 4, 2012

    Availability

    Academic Institutions and Nonprofits only

26. pET28a-ybbr-HIS-ddFLN4-Fgß

    Plasmid

    #101719

    Purpose

    wildtype Fgß peptide with ddFLN4 fingerprint domain with N-terminal ybbr tag for covalent immobilization - resulting in weak forces

    Depositor

    Hermann Gaub

    Article

    Milles et al Science. 2018 Mar 30;359(6383):1527-1533

    Insert

    ddFLN4 fingerprint, Fgß peptide (FGB Human, Dictyostelium discoideum)
    

    Tags

    HIS and ybbr

    Expression

    Bacterial

    Mutation

    C18S in ddFLN4

    Promoter

    T7

    Available Since

    March 29, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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27. BPV00605 pET21GG2-His(6)-BECN(1-265)

    Plasmid

    #136686

    Purpose

    Expresses the Beclin-1 IDR+CC domains (a.a. 1-265) with a N-terminal hexa-Histidine tag

    Depositor

    Colin Garvie

    Article

    Ranaghan et al Biochemistry. 2017 Dec 26;56(51):6639-66

    Insert

    Beclin-1 IDR + CC (BECN1 Human)
    

    Tags

    Hexa-Histadine

    Expression

    Bacterial

    Mutation

    DNA optimized for E. coli expression

    Promoter

    T7

    Available Since

    Feb. 14, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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28. PET-B-SpCas9-HF1-NLS-6xHis

    Plasmid

    #207385

    Purpose

    Expression of increased-fidelity (i.e. high-fidelity) SpCas9 variant in bacterial cells

    Depositor

    Ervin Welker

    Article

    Kulcsar et al Nat Commun. 2023 Sep 16;14(1):5746. doi:

    Insert

    B-SpCas9-HF1
    

    Use

    CRISPR

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    N497A, R661A, Q695A, Q926A, amino acids 1005-1013…

    Promoter

    T7

    Available Since

    Sept. 25, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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29. HIV-1 integrase F185H/C280S in pET15b

    Plasmid

    #61672

    Purpose

    HIV-1 integrase F185H/C280S in pET-15b

    Depositor

    Robert Craigie

    Article

    Jenkins et al J Biol Chem. 1996 Mar 29;271(13):7712-8.

    Insert

    HIV-1 integrase F185H/C280S
    

    Use

    Synthetic Biology

    Tags

    His

    Promoter

    T7 promotor

    Available Since

    Feb. 11, 2015

    Availability

    Academic Institutions and Nonprofits only

30. human KANK1 Ankyrin Repeats pET151

    Plasmid

    #164053

    Purpose

    Expresses human KANK1 Ank domain in bacterial cells

    Depositor

    Ben Goult

    Insert

    Kidney Ankyrin Repeat-Containing Protein 1 (KANK1 Human)
    

    Tags

    His-tag, TEV cleavage site

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Oct. 6, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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