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pcDNA3.1-3xFLAG-V5-ccdB

Plasmid

#87064

Purpose

Mammalian Gateway-compatible expression vector backbone with an N-terminal 3xFLAG-V5 tag

Depositor

Susan Lindquist, Mikko Taipale

Article

Taipale et al Cell. 2012 Aug 31;150(5):987-1001. doi:

Type

Empty backbone

Tags

3xFLAG-V5

Expression

Mammalian

Available Since

March 7, 2017

Availability

Academic Institutions and Nonprofits only

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pSLQ2817 pPB: CAG-PYL1-VPR-IRES-Puro-WPRE-SV40PA PGK-ABI-tagBFP-SpdCas9

Plasmid

#84239

Purpose

Expresses ABA-inducible VPR-Sp dCas9

Depositor

Stanley Qi

Article

Gao et al Nat Methods. 2016 Oct 24. doi: 10.1038/n

Inserts

ABI-tagBFP-Sp dCas9
PYL1-VPR

Use

CRISPR and Synthetic Biology; Piggybac

Tags

2xNLS (SV40), ABI, HA tag, IRES-Puro, PYL1, and t…

Expression

Mammalian

Promoter

CAG and PGK

Available Since

Nov. 9, 2016

Availability

Academic Institutions and Nonprofits only

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pLH-sgRNA1-2XPP7

Plasmid

#75390

Purpose

sgRNA1-2XPP7

Depositor

Thoru Pederson

Article

Ma et al Nat Biotechnol. 2016 Apr 18. doi: 10.103

Insert

sgRNA1-2XPP7

Use

CRISPR and Lentiviral

Tags

no

Expression

Mammalian

Promoter

U6

Available Since

May 25, 2016

Availability

Academic Institutions and Nonprofits only

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pORTMAGE-3

Plasmid

#72678

Purpose

Expresses Lambda Red recombinases and a dominant negative MutL allele all controlled by temperature sensitve cI857 repressor for high precision and efficiency MAGE experiments. Kan resistance marker.

Depositor

Csaba Pál

Article

Nyerges et al Proc Natl Acad Sci U S A. 2016 Feb 16. p

Insert

mutL E32K

Tags

none

Expression

Bacterial

Mutation

E32K mutation conferring dominant mutator phenoty…

Available Since

Feb. 18, 2016

Availability

Academic Institutions and Nonprofits only

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pRetroX-Tight-MCS_PGK-GpNLuc

Plasmid

#70185

Purpose

Constiutive mammalian expression of the bioluminescent GpNLuc LumiFluor reporter for single-cell analysis and sensitive optical imaging of living subjects.

Depositor

Antonio Amelio

Article

Schaub et al Cancer Res. 2015 Sep 30. pii: canres.353

Type

Empty backbone

Use

Retroviral

Expression

Mammalian

Promoter

PGK

Available Since

Dec. 2, 2015

Availability

Academic Institutions and Nonprofits only

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pDEST-FRT/T0-Flag-BRCA1

Plasmid

#71117

Purpose

mammalian expression of Flag-BRCA1 siRNA resistant construct

Depositor

Daniel Durocher

Article

Orthwein et al Nature. 2015 Dec 9. doi: 10.1038/nature1

Insert

BRCA1 (BRCA1 Human)

Tags

Flag

Expression

Mammalian

Promoter

CMV/TO

Available Since

Nov. 12, 2015

Availability

Industry, Academic Institutions, and Nonprofits

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CAG-FLEx(FRT)-TC

Plasmid

#67827

Purpose

Flp-dependent expression of TVA-mCherry fusion to mediate modifed rabies virus expression in cells

Depositor

Liqun Luo

Article

Schwarz et al Nature. 2015 Aug 6;524(7563):88-92. doi:

Insert

TVA-mCherry fusion

Use

AAV

Expression

Mammalian

Available Since

Aug. 10, 2015

Availability

Academic Institutions and Nonprofits only

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Str-KDEL_SBP-EGFP-GPI

Plasmid

#65294

Purpose

synchronize trafficking of EGFP-GPI from the ER (RUSH system)

Depositor

Franck Perez

Article

Boncompain et al Nat Methods. 2012 Mar 11;9(5):493-8. doi

Insert

GPI anchor

Tags

EGFP and IL-2 Signal Sequence and Streptavidin Bi…

Expression

Mammalian

Promoter

CMV

Available Since

June 3, 2015

Availability

Academic Institutions and Nonprofits only

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pMDIAI

Plasmid

#51655

Purpose

E coli genetic modification

Depositor

Sheng Yang

Article

Yang et al Appl Environ Microbiol. 2014 Jul 1;80(13

Insert

apramycin antibiotic resistance gene

Use

Genetic modification

Promoter

unknown

Available Since

June 24, 2014

Availability

Academic Institutions and Nonprofits only

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pACYCT2

Plasmid

#45799

Depositor

Luc Ponchon

Article

Ponchon et al Nucleic Acids Res. 2013 Aug;41(15):e150.

Type

Empty backbone

Expression

Bacterial

Available Since

Sept. 5, 2013

Availability

Academic Institutions and Nonprofits only

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pCMV-Pam-FL

Plasmid

#42570

Depositor

Vijaya Ramesh

Article

Santos et al J Neurosci Res. 2006 Feb 1;83(2):222-32.

Insert

human MYCBP2 (MYCBP2 Human)

Tags

Myc

Expression

Mammalian

Mutation

Pam-FL clone lacks alternatively spliced exons 52…

Promoter

CMV

Available Since

March 14, 2013

Availability

Academic Institutions and Nonprofits only

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pMXs-IP-EGFP-ULK1

Plasmid

#38193

Depositor

Noboru Mizushima

Article

Hara et al J Cell Biol. 2008 May 5. 181(3):497-510.

Insert

a novel protein kinase structurally related to C. elegans UNC-51 (Ulk1 Mouse)

Use

Retroviral

Tags

EGFP

Expression

Mammalian

Available Since

Nov. 27, 2012

Availability

Academic Institutions and Nonprofits only

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pCXN2-BCL6 (CXN-BCL6)

Plasmid

#40346

Depositor

Alexander Dent

Article

Vasanwala et al J Immunol. 2002 Aug 15;169(4):1922-9.

Insert

BCL6 (BCL6 Human)

Tags

FLAG

Expression

Mammalian

Promoter

pCAG

Available Since

Oct. 15, 2012

Availability

Academic Institutions and Nonprofits only

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GFP-Rab5CA (Q79L)

Plasmid

#35140

Depositor

Sergio Grinstein

Article

Bohdanowicz et al Mol Biol Cell. 2012 Jan;23(1):176-87. Ep

Insert

Rab5a (RAB5A Human)

Tags

EGFP

Expression

Mammalian

Mutation

Constitutively Active- Q79L

Promoter

CMV

Available Since

March 20, 2012

Availability

Academic Institutions and Nonprofits only

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pDONR223-ERBB4

Plasmid

#23875

Depositor

William Hahn, David Root

Article

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

ERBB4 (ERBB4 Human)

Use

Gateway Cloning

Available Since

July 30, 2010

Availability

Academic Institutions and Nonprofits only

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pDONR223-FLT1

Plasmid

#23912

Depositor

William Hahn, David Root

Article

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

FLT1 (FLT1 Human)

Use

Gateway Cloning

Mutation

S755P

Available Since

July 30, 2010

Availability

Academic Institutions and Nonprofits only

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pDONR223-FGFR3

Plasmid

#23933

Depositor

William Hahn, David Root

Article

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

FGFR3 (FGFR3 Human)

Use

Gateway Cloning

Available Since

July 30, 2010

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1hSIRT3_H248Y_HA

Plasmid

#24492

Depositor

Eric Verdin

Article

Hirschey et al Nature. 2010 Mar 4. 464(7285):121-5.

Insert

Human Sirtuin 3 catalytically inactive mutant (SIRT3 Human)

Tags

HA

Expression

Mammalian

Mutation

H248Y

Available Since

April 23, 2010

Availability

Academic Institutions and Nonprofits only

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pWS-TK6

Plasmid

#20350

Depositor

Mario Capecchi

Article

Wu et al Nat Protoc. 2008 . 3(6):1056-76.

Type

Empty backbone

Use

Mouse Targeting

Available Since

March 11, 2009

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1/Myc-His(-)-HSulf-2

Plasmid

#13004

Depositor

Steven Rosen

Article

Morimoto-Tomita et al J Biol Chem. 2002 Dec 20. 277(51):49175-

Insert

human sulfatase 2 (SULF2 Human)

Tags

6x His and Myc

Expression

Mammalian

Available Since

Oct. 30, 2006

Availability

Academic Institutions and Nonprofits only

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