4,079 results

1. pX330A-1x2

   Plasmid

   #58766

   Purpose

   Expresses Cas9 nuclease and gRNA

   Depositor

   Takashi Yamamoto

   Article

   Sakuma et al Sci Rep. 2014 Jun 23;4:5400. doi: 10.103

   Insert

   humanized S. pyogenes Cas9 nuclease
   

   Use

   CRISPR

   Tags

   3xFLAG

   Expression

   Mammalian

   Mutation

   Promoter

   CBh

   Available since

   Nov. 7, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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2. pcDNA3-myc-CUL3

   Plasmid

   #19893

   Depositor

   Yue Xiong

   Article

   Ohta et al Mol Cell. 1999 Apr . 3(4):535-41.

   Insert

   cullin 3 (CUL3 Human)
   

   Use

   Tags

   3xmyc

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Dec. 31, 2008

   Availability

   Academic Institutions and Nonprofits only

   

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3. pEGFP-Atg14L

   Plasmid

   #21635

   Depositor

   Tamotsu Yoshimori

   Article

   Matsunaga et al Nat Cell Biol. 2009 Apr . 11(4):385-96.

   Insert

   Atg14L (ATG14 Human)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 5, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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4. mPiezo1-IRES-eGFP

   Plasmid

   #80925

   Purpose

   expresses mouse Piezo1 with GFP

   Depositor

   Ardem Patapoutian

   Article

   Coste et al Science. 2010 Oct 1;330(6000):55-60. doi

   Insert

   mouse Piezo1 CDS (Piezo1 Mouse)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 25, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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5. mEGFP-N1

   Plasmid

   #54767

   Purpose

   Localization: N1 Cloning Vector, Excitation: 488, Emission: 507

   Depositor

   Michael Davidson

   Has Service

   Cloning Grade DNA

   Type

   Empty backbone

   Use

   Tags

   mEGFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   June 20, 2014

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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6. ER-GCaMP6-150

   Plasmid

   #86918

   Purpose

   Fluorescent reporter for ER calcium signaling

   Depositor

   Timothy Ryan

   Article

   de Juan-Sanz et al Neuron. 2017 Jan 30. pii: S0896-6273(17)

   Insert

   ER-GCaMP6-150
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   K78H, T302L, R303P, A317E, D324G, D360G, D380Y, T…

   Promoter

   CAMKII

   Available since

   March 15, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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7. AAV:ITR-U6-sgRNA(Kras)-U6-sgRNA(p53)-U6-sgRNA(Lkb1)-pEFS-Rluc-2A-Cre-shortPA-KrasG12D_HDRdonor-ITR (AAV-KPL)

   Plasmid

   #60224

   Purpose

   Expresses Cre recombinase and Renilla luciferase from the EFS promoter and three U6-driven sgRNAs targeting Kras, p53, and Lkb1. Contains KrasG12D HDR donor. AAV backbone.

   Depositor

   Feng Zhang

   Article

   Platt et al Cell. 2014 Sep 24. pii: S0092-8674(14)01

   Inserts

   sgRNA
   Renilla luciferase
   Cre recombinase
   KrasG12D HDR donor (Kras Mouse)
   

   Use

   AAV, CRISPR, Cre/Lox, and Mouse Targeting

   Tags

   Cre-HA and Rluc-P2A

   Expression

   Mammalian

   Mutation

   Promoter

   EFS, No promoter, and U6

   Available since

   Oct. 9, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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8. pY094

   Plasmid

   #84743

   Purpose

   Expresses huAsCpf1-T2A-GFP and crRNA guide

   Depositor

   Feng Zhang

   Article

   Zetsche et al Nat Biotechnol. 2017 Jan;35(1):31-34. do

   Inserts

   huAsCpf1
   GFP
   

   Use

   Tags

   3xHA, NLS, and T2A

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Jan. 25, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. pLX_311-KRAB-dCas9

   Plasmid

   #96918

   Purpose

   for CRISPRi, lentiviral expression of KRAB-dCas9 and BlastR. Also called pXPR_121

   Depositor

   David Root, William Hahn, John Doench

   Article

   Rosenbluh et al Nat Commun. 2017 May 23;8:15403. doi: 10

   Insert

   dCas9
   

   Use

   Lentiviral

   Tags

   KRAB

   Expression

   Mammalian

   Mutation

   dCas9

   Promoter

   EF1a

   Available since

   June 28, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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10. sgRNA(MS2) cloning backbone

    Plasmid

    #61424

    Purpose

    sgRNA cloning backbone with MS2 loops at tetraloop and stemloop 2. Contains BbsI sites for insertion of spacer sequences.

    Depositor

    Feng Zhang

    Article

    Konermann et al Nature. 2014 Dec 10. doi: 10.1038/nature

    Has Service

    Cloning Grade DNA

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Dec. 15, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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