We narrowed to 15,050 results for: pET

1. FL MALT1 Catalytic Mutant in pET29b-Strep

   Plasmid

   #48969

   Purpose

   Expresses Full length catalytic mutant MALT1 in e.coli

   Depositor

   Guy Salvesen

   Article

   Hachmann et al Biochem J. 2012 Apr 1;443(1):287-95. doi

   Insert

   MALT1 isoform A (MALT1 Human)
   

   Tags

   His and Strep

   Expression

   Bacterial

   Mutation

   Catalytic Mutant C464A

   Promoter

   T7

   Available Since

   Nov. 6, 2013

   Availability

   Academic Institutions and Nonprofits only

2. pET-3a-His-MBP-TEV-VC_Cas1_WT

   Plasmid

   #120364

   Purpose

   Expresses wildtype V-C Cas1

   Depositor

   Jennifer Doudna

   Article

   Wright et al Mol Cell. 2019 Feb 21;73(4):727-737.e3.

   Insert

   VC_Cas1
   

   Use

   CRISPR

   Expression

   Bacterial

   Available Since

   April 12, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pET-3a-His-MBP-TEV-VD_Cas1_WT

   Plasmid

   #120365

   Purpose

   Expresses wildtype V-D Cas1

   Depositor

   Jennifer Doudna

   Article

   Wright et al Mol Cell. 2019 Feb 21;73(4):727-737.e3.

   Insert

   VD_Cas1
   

   Use

   CRISPR

   Expression

   Bacterial

   Available Since

   April 12, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. HOXB4 (human) HA-TAT-tag pET

   Plasmid

   #8526

   Depositor

   Corey Largman

   Article

   Shen et al Proc Natl Acad Sci U S A 1989 Nov;86(21)

   Insert

   HOXB4 (HOXB4 Human)
   

   Tags

   HA

   Expression

   Bacterial

   Mutation

   replace T7 and HIS tags of pET with an HA tag;

   Available Since

   May 30, 2006

   Availability

   Academic Institutions and Nonprofits only

5. HOXB7 (human) HIS-tag pET

   Plasmid

   #8538

   Depositor

   Corey Largman

   Article

   Shen et al Proc Natl Acad Sci U S A 1989 Nov;86(21)

   Insert

   HOXB7 (HOXB7 Human)
   

   Tags

   His and T7

   Expression

   Bacterial

   Available Since

   May 30, 2006

   Availability

   Academic Institutions and Nonprofits only

6. pET51b(+)-NADPH-LUCID-4 (C50 6.5 uM)

   Plasmid

   #117911

   Purpose

   Plasmid encoding the bacterial expression of the BRET semi-synthetic biosensor measuring the NADPH concentration with a C50 of 6.5 uM

   Depositor

   Kai Johnsson

   Article

   Yu et al Science. 2018 Sep 14;361(6407):1122-1126

   Insert

   SNAP_Pro30_[cpNLuc23_24]eDHFR(R44A-H45Q)
   

   Tags

   His-tag and Strep-tag

   Expression

   Bacterial

   Mutation

   R44A & H45Q on eDHFR

   Promoter

   T7

   Available Since

   Oct. 25, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. pET-45b-NFYC-IDR-mEGFP

   Plasmid

   #185015

   Purpose

   Bacterial Expression of 6xHis NFYC IDR Sirius-GFP Fusion Protein

   Depositor

   Denes Hnisz

   Article

   Asimi et al Nat Genet. 2022 Aug;54(8):1238-1247. doi

   Insert

   NFYC IDR (Nfyc Mouse)
   

   Expression

   Bacterial

   Available Since

   June 21, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. pET29-hMIGA2(306-593)-6xhis

   Plasmid

   #192837

   Purpose

   Express codon-optimized human MIGA2 fragment 306-593 with a C-terminal 6xhis tag (lipid transfer construct) in bacteria

   Depositor

   Karin Reinisch

   Article

   Hong et al J Cell Biol. 2022 Dec 5;221(12):e2022070

   Insert

   Codon-optimized human Mitoguardin 2 (MIGA2 Human)
   

   Tags

   6xhis

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Dec. 15, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. HOXB1 (human) HIS-tag pET

   Plasmid

   #8520

   Depositor

   Corey Largman

   Article

   Shen et al Nucleic Acids Res 1996 Mar 1;24(5):898-9

   Insert

   HOXB1 (HOXB1 Human)
   

   Tags

   His and T7

   Expression

   Bacterial

   Mutation

   PCR from cDNA clone

   Available Since

   July 6, 2006

   Availability

   Academic Institutions and Nonprofits only

10. pET-28a-His6-SUMO-VAMP8

    Plasmid

    #135553

    Purpose

    Express mouse VAMP8 in E. coli BL21, purified via HisPur Ni-NTA Resin

    Depositor

    Jingshi Shen

    Article

    Yu et al Methods Mol Biol. 2019;1860:237-249. doi

    Insert

    VAMP8
    

    Tags

    6xHis tag, thrombin recognition and cleavage site…

    Expression

    Bacterial

    Mutation

    No ATG start codon

    Promoter

    T7 promoter

    Available Since

    March 2, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. pET22b-ecDHFR-N23PP/L28F/G51PEKN

    Plasmid

    #118585

    Purpose

    Bacterial expression of E.coli DHFR N23PP, L28F, and G51PEKN mutant

    Depositor

    Stephen Benkovic

    Article

    Liu et al Proc Natl Acad Sci U S A. 2013 Jun 18;11

    Insert

    DHFR
    

    Expression

    Bacterial

    Mutation

    N23 mutated to P23, L28 mutated to F28, G51 mutat…

    Promoter

    T7

    Available Since

    Nov. 29, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. HIV-1 integrase W235F in pET-15b

    Plasmid

    #61681

    Purpose

    HIV-1 integrase W235F in pET-15b

    Depositor

    Robert Craigie

    Article

    Li et al EMBO J. 2006 Mar 22;25(6):1295-304. Epub

    Insert

    HIV-1 integrase W235F
    

    Tags

    His

    Mutation

    W235F

    Promoter

    T7

    Available Since

    Feb. 11, 2015

    Availability

    Academic Institutions and Nonprofits only

13. pET28a SUMO Nup53xl 162-267

    Plasmid

    #86002

    Purpose

    bacterial expression of his6 and SUMO tagged RRM motif of Xenopus laevis Nup53

    Depositor

    Wolfram Antonin

    Type

    Empty backbone

    Tags

    SUMO

    Expression

    Bacterial

    Available Since

    April 11, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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14. HOXA9 (murine) HIS-tag pET

    Plasmid

    #8492

    Depositor

    Corey Largman

    Article

    Shen et al Mol Cell Biol 1997 Nov;17(11):6448-58.

    Insert

    HoxA9 (Hoxa9 Mouse)
    

    Tags

    His and T7

    Expression

    Bacterial

    Mutation

    BamHI/BalI fusion into amino acid #2 of HOXA9; ma…

    Available Since

    May 30, 2006

    Availability

    Academic Institutions and Nonprofits only

15. HOXB3 (human) HIS-tag pET

    Plasmid

    #8524

    Depositor

    Corey Largman

    Article

    Shen et al Nucleic Acids Res 1996 Mar 1;24(5):898-9

    Insert

    HOXB3 (HOXB3 Human)
    

    Tags

    His and T7

    Expression

    Bacterial

    Mutation

    Contains 5 additional amino acids before ATG from…

    Available Since

    May 30, 2006

    Availability

    Academic Institutions and Nonprofits only

16. HOXC8 (murine) HIS-tag pET

    Plasmid

    #8567

    Depositor

    Corey Largman

    Insert

    HOXC8 (Hoxc8 Mouse)
    

    Tags

    His and T7

    Expression

    Bacterial

    Mutation

    makes protein by Western

    Available Since

    May 30, 2006

    Availability

    Academic Institutions and Nonprofits only

17. HOXC6 (human) HIS-tag pET

    Plasmid

    #8565

    Depositor

    Corey Largman

    Insert

    HOXC6 (HOXC6 Human)
    

    Tags

    His and T7

    Expression

    Bacterial

    Mutation

    This protein starts at an internal Met initially …

    Available Since

    May 30, 2006

    Availability

    Academic Institutions and Nonprofits only

18. pETDUET1-hUCH37 (ISF1) - hNFRKB (1-156)

    Plasmid

    #61939

    Purpose

    Co-expression of hUCH37 (ISF1) and hNFRKB (1-156) in E.coli

    Depositor

    Chris Hill

    Article

    VanderLinden et al Mol Cell. 2015 Feb 17. pii: S1097-2765(1

    Inserts

    hUCH37 (ISF1) (UCHL5 Human)
    hNFRKB (1-156)
    

    Tags

    6x HIS

    Expression

    Bacterial

    Mutation

    Construct ends at residue 156

    Promoter

    T7

    Available Since

    Oct. 9, 2015

    Availability

    Academic Institutions and Nonprofits only

19. pETDUET1-hUCH37 (ISF2)-NFRKB(39-156)

    Plasmid

    #61938

    Purpose

    Co-expression of hUCH37 (ISF2) and hNFRKB (39-156) in E.coli

    Depositor

    Chris Hill

    Article

    VanderLinden et al Mol Cell. 2015 Feb 17. pii: S1097-2765(1

    Inserts

    hUCH37 (ISF2) (UCHL5 Human)
    hNFRKB (39-156) (NFRKB Human)
    

    Tags

    6x HIS

    Expression

    Bacterial

    Mutation

    Construct ends at residue 156, residues 2-38 dele…

    Promoter

    T7

    Available Since

    Oct. 9, 2015

    Availability

    Academic Institutions and Nonprofits only

20. pET30a-ASXL1-HARE-HTH domain

    Plasmid

    #107164

    Purpose

    Expresses murine ASXL1 HARE-HTH domain (amino acids 2-83) in E. coli

    Depositor

    Douglas Feldman

    Article

    Kweon et al Mol Cell. 2019 Apr 5. pii: S1097-2765(19

    Insert

    ASXL1 (Asxl1 Mouse)
    

    Tags

    Flag

    Expression

    Bacterial

    Mutation

    Contains amino acids 2-83

    Promoter

    T7

    Available Since

    April 20, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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21. pET28a-FgßF3-ddFLN4-HIS-ybbr

    Plasmid

    #101743

    Purpose

    mutant FgßF3 peptide (additional phenylalanine) with ddFLN4 fingerprint domain with C-terminal ybbr tag for covalent immobilization - resulting in high forces

    Depositor

    Hermann Gaub

    Article

    Milles et al Science. 2018 Mar 30;359(6383):1527-1533

    Insert

    FgßF3 peptide, ddFLN4 fingerprint (FGB Human, Dictyostelium discoideum)
    

    Tags

    HIS and ybbr

    Expression

    Bacterial

    Mutation

    C18S in ddFLN4, added 9F in FgßF3

    Promoter

    T7

    Available Since

    March 29, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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22. pET151-GFP-PP-RPN2 (925-953)

    Plasmid

    #73744

    Purpose

    Expression of GFP-PP-RPN2 (925-953) fusion in E.coli

    Depositor

    Chris Hill

    Article

    VanderLinden et al J Biol Chem. 2017 Apr 25. pii: jbc.M117.

    Insert

    RPN2 (RPN2 Human)
    

    Tags

    6x His and GFP

    Expression

    Bacterial

    Mutation

    Construct possesses only residues 932-953 of RPN2

    Promoter

    T7

    Available Since

    April 27, 2017

    Availability

    Academic Institutions and Nonprofits only

23. delNC C-His Ana GvpC in pET28a

    Plasmid

    #85736

    Purpose

    Expresses a truncated His-tagged GvpC from Anabaena flos-aquae without the N-and C-terminal regions in E.coli

    Depositor

    Mikhail Shapiro

    Article

    Lakshmanan et al ACS Nano. 2016 Aug 23;10(8):7314-22. doi

    Insert

    Gas vesicle protein C (truncated and his-tagged)
    

    Tags

    His-tag

    Expression

    Bacterial

    Mutation

    Deletion of N and C terminal regions, addition of…

    Promoter

    T7

    Available Since

    March 29, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pET15b/GgVcl 884-1066 (aka Vt)

    Plasmid

    #46176

    Depositor

    Susan Craig

    Article

    Steimle et al J Biol Chem. 1999 Jun 25;274(26):18414-2

    Insert

    Vcl 884-1066 (VCL Chicken)
    

    Tags

    His

    Expression

    Bacterial

    Mutation

    aa 884-1066 (tail domain)

    Promoter

    T7

    Available Since

    July 10, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pET51b-His-TEV-SNAP-tag_cx

    Plasmid

    #167273

    Purpose

    Overexpression of crystallizing SNAP-tag variant (P179R) in E. coli

    Depositor

    Kai Johnsson

    Article

    Wilhelm et al Biochemistry. 2021 Aug 24;60(33):2560-25

    Insert

    SNAP-tag
    

    Tags

    His x10

    Expression

    Bacterial

    Mutation

    P179R

    Promoter

    T7

    Available Since

    April 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pET28a-ybbR-HIS-CBM-CohI

    Plasmid

    #58709

    Purpose

    Probing molecule to perform single molecule force spectroscopy experiments

    Depositor

    Hermann Gaub

    Article

    Otten et al Nat Methods. 2014 Sep 7. doi: 10.1038/nm

    Insert

    CBM-CohI
    

    Tags

    HIS, HRV 3C, and ybbR

    Expression

    Bacterial

    Mutation

    changed Alanine 2 to Cysteine in CBM domain

    Promoter

    T7

    Available Since

    Aug. 25, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. HOXB13 (human) HIS-tag pET

    Plasmid

    #8543

    Depositor

    Corey Largman

    Article

    Stelnicki et al J Invest Dermatol 1998 Jul;111(1):57-63.

    Insert

    HOXB13 (HOXB13 Human)
    

    Tags

    His and T7

    Expression

    Bacterial

    Mutation

    makes protein by Western and by transcription/tra…

    Available Since

    May 30, 2006

    Availability

    Academic Institutions and Nonprofits only

28. HOXD12 (murine) HIS-tag pET

    Plasmid

    #8553

    Depositor

    Corey Largman

    Article

    Shen et al Mol Cell Biol 1997 Nov;17(11):6448-58.

    Insert

    HOXD12 (Hoxd12 Mouse)
    

    Tags

    His and T7

    Expression

    Bacterial

    Mutation

    Fusion sequence is: ggatccATG

    Available Since

    May 30, 2006

    Availability

    Academic Institutions and Nonprofits only

29. p139 pETF-GST RanBP10 L301I

    Plasmid

    #11092

    Depositor

    Ramesh Shivdasani

    Insert

    RanBP10 (Ranbp10 Mouse)
    

    Tags

    GST

    Expression

    Bacterial

    Mutation

    L301I; see depositor comments below

    Available Since

    Dec. 16, 2005

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. HOXD4 (murine) HIS-tag pET

    Plasmid

    #8548

    Depositor

    Corey Largman

    Article

    Shen et al Nucleic Acids Res 1996 Mar 1;24(5):898-9

    Insert

    HOXD4 (Hoxd4 Mouse)
    

    Tags

    His and T7

    Expression

    Bacterial

    Mutation

    makes protein by Western and by in vitro transcri…

    Available Since

    May 30, 2006

    Availability

    Academic Institutions and Nonprofits only