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pLsCas13a-gRNA-3

Plasmid

#164867

Purpose

Constitutive expression of single-spacer CRISPR array with spacer #3 targeting deGFP mRNA for LsCas13a in bacteria.

Depositor

Chase Beisel

Article

Wandera et al Methods Mol Biol. 2022;2404:135-153. doi

Insert

LsCas13a-gRNA-3

Use

CRISPR

Expression

Bacterial

Promoter

J23119

Available Since

Feb. 22, 2021

Availability

Academic Institutions and Nonprofits only

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pLsCas13a-gRNA-2

Plasmid

#164866

Purpose

Constitutive expression of single-spacer CRISPR array with spacer #2 targeting deGFP mRNA for LsCas13a in bacteria.

Depositor

Chase Beisel

Article

Wandera et al Methods Mol Biol. 2022;2404:135-153. doi

Insert

LsCas13a-gRNA-2

Use

CRISPR

Expression

Bacterial

Promoter

J23119

Available Since

Feb. 22, 2021

Availability

Academic Institutions and Nonprofits only

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OA-1050I (notch)

Plasmid

#132421

Purpose

express arrays of gRNA targeting Notch under dU6-3 promoter

Depositor

Omar Akbari

Article

Buchman et al CRISPR J. 2020 Jun;3(3):164-176. doi: 10

Insert

notch gRNA array

Expression

Insect

Promoter

U6-3

Available Since

Sept. 15, 2020

Availability

Academic Institutions and Nonprofits only

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OA-1050K (firefly luciferase)

Plasmid

#132422

Purpose

express arrays of gRNA targeting Firefly Luciferase under dU6-3 promoter

Depositor

Omar Akbari

Article

Buchman et al CRISPR J. 2020 Jun;3(3):164-176. doi: 10

Insert

firefly Luciferase gRNA array

Expression

Insect

Promoter

U6-3

Available Since

Sept. 15, 2020

Availability

Academic Institutions and Nonprofits only

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OA-1050U (cinnabar)

Plasmid

#132423

Purpose

express arrays of gRNA targeting Cinnabar under dU6-3 promoter

Depositor

Omar Akbari

Article

Buchman et al CRISPR J. 2020 Jun;3(3):164-176. doi: 10

Insert

cinnabar gRNA array

Expression

Insect

Promoter

U6-3

Available Since

Sept. 15, 2020

Availability

Academic Institutions and Nonprofits only

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OA-1050V (wingless)

Plasmid

#132424

Purpose

express arrays of gRNA targeting Wingless under dU6-3 promoter

Depositor

Omar Akbari

Article

Buchman et al CRISPR J. 2020 Jun;3(3):164-176. doi: 10

Insert

wingless gRNA array

Expression

Insect

Promoter

U6-3

Available Since

Sept. 15, 2020

Availability

Academic Institutions and Nonprofits only

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OA-1050Z4 (yellow)

Plasmid

#132425

Purpose

express arrays of gRNA targeting Yellow under dU6-3 promoter

Depositor

Omar Akbari

Article

Buchman et al CRISPR J. 2020 Jun;3(3):164-176. doi: 10

Insert

yellow gRNA array

Expression

Insect

Promoter

U6-3

Available Since

Sept. 15, 2020

Availability

Academic Institutions and Nonprofits only

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pGEL087

Plasmid

#137895

Purpose

iSTU-CRISPR/Cas12a guide RNA unit insertion vector with OsCDPK2 intron

Depositor

Yong Zhang

Article

Zhong et al Rice (N Y). 2020 Feb 3;13(1):8. doi: 10.

Type

Empty backbone

Use

CRISPR

Expression

Plant

Available Since

Aug. 31, 2020

Availability

Academic Institutions and Nonprofits only

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pGEL052

Plasmid

#137906

Purpose

OsBiP1 promoter driven Cas9-tRNA system

Depositor

Yong Zhang

Article

Ren et al Front Plant Sci. 2019 Sep 20;10:1173. do

Type

Empty backbone

Use

CRISPR

Expression

Plant

Available Since

Aug. 31, 2020

Availability

Academic Institutions and Nonprofits only

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pGEL053

Plasmid

#137907

Purpose

OsBiP1 promoter driven Cas9-csy4 system

Depositor

Yong Zhang

Article

Ren et al Front Plant Sci. 2019 Sep 20;10:1173. do

Type

Empty backbone

Use

CRISPR

Expression

Plant

Available Since

Aug. 31, 2020

Availability

Academic Institutions and Nonprofits only

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pGEL050

Plasmid

#137904

Purpose

Dual-mini35S driven Cas9-Csy4 system

Depositor

Yong Zhang

Article

Ren et al Front Plant Sci. 2019 Sep 20;10:1173. do

Type

Empty backbone

Use

CRISPR

Expression

Plant

Available Since

Feb. 25, 2020

Availability

Academic Institutions and Nonprofits only

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pGEL051

Plasmid

#137905

Purpose

Dual-mini35S-enhancer driven Cas9-Csy4 system

Depositor

Yong Zhang

Article

Ren et al Front Plant Sci. 2019 Sep 20;10:1173. do

Type

Empty backbone

Use

CRISPR

Expression

Plant

Available Since

Feb. 25, 2020

Availability

Academic Institutions and Nonprofits only

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sgRNA HBB

Plasmid

#128120

Purpose

Co-expresses sgRNA HBB, SpyCas9 scaffold (F+E) and GFP (transfection marker)

Depositor

Dominik Niopek

Article

Aschenbrenner et al Sci Adv. 2020 Feb 5;6(6):eaay0187. doi:

Insert

sgRNA HBB + GFP

Use

CRISPR and Synthetic Biology

Expression

Mammalian

Promoter

RSV/U6

Available Since

Feb. 13, 2020

Availability

Academic Institutions and Nonprofits only

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sgRNA RUNX

Plasmid

#128122

Purpose

Co-expresses sgRNA RUNX, SpyCas9 scaffold (F+E) and GFP (transfection marker)

Depositor

Dominik Niopek

Article

Aschenbrenner et al Sci Adv. 2020 Feb 5;6(6):eaay0187. doi:

Insert

sgRNA RUNX + GFP

Use

CRISPR and Synthetic Biology

Expression

Mammalian

Promoter

RSV/U6

Available Since

Feb. 13, 2020

Availability

Academic Institutions and Nonprofits only

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sgRNA HEK

Plasmid

#128123

Purpose

Co-expresses sgRNA HEK, SpyCas9 scaffold (F+E) and GFP (transfection marker)

Depositor

Dominik Niopek

Article

Aschenbrenner et al Sci Adv. 2020 Feb 5;6(6):eaay0187. doi:

Insert

sgRNA HEK + GFP

Use

CRISPR and Synthetic Biology

Expression

Mammalian

Promoter

RSV/U6

Available Since

Feb. 13, 2020

Availability

Academic Institutions and Nonprofits only

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PX330_ZNF503_2

Plasmid

#135755

Purpose

Encodes gRNA for 3' target of human ZNF503

Depositor

Richard M. Myers

Article

Savic et al Genome Res. 2015 Sep 9.

Insert

ZNF503 gRNA (ZNF503 Human)

Use

CRISPR

Promoter

U6

Available Since

Jan. 28, 2020

Availability

Academic Institutions and Nonprofits only

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PX330_ONECUT2_2

Plasmid

#135760

Purpose

Encodes gRNA for 3' target of human ONECUT2

Depositor

Richard M. Myers

Article

Savic et al Genome Res. 2015 Sep 9.

Insert

ONECUT2 gRNA (ONECUT2 Human)

Use

CRISPR

Promoter

U6

Available Since

Jan. 28, 2020

Availability

Academic Institutions and Nonprofits only

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PX330_ONECUT2_1

Plasmid

#135759

Purpose

Encodes gRNA for 3' target of human ONECUT2

Depositor

Richard M. Myers

Article

Savic et al Genome Res. 2015 Sep 9.

Insert

ONECUT2 gRNA (ONECUT2 Human)

Use

CRISPR

Promoter

U6

Available Since

Jan. 27, 2020

Availability

Academic Institutions and Nonprofits only

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PX458_ZBTB21_1

Plasmid

#135741

Purpose

Encodes gRNA for 3' target of human ZBTB21

Depositor

Richard M. Myers

Article

Savic et al Genome Res. 2015 Sep 9.

Insert

ZBTB21 gRNA (ZBTB21 Human)

Use

CRISPR

Promoter

U6

Available Since

Jan. 27, 2020

Availability

Academic Institutions and Nonprofits only

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PX330_MLLT10_iso1_1

Plasmid

#135745

Purpose

Encodes gRNA for 3' target of human MLLT10_iso1

Depositor

Richard M. Myers

Article

Savic et al Genome Res. 2015 Sep 9.

Insert

MLLT10_iso1 gRNA (MLLT10 Human)

Use

CRISPR

Promoter

U6

Available Since

Jan. 24, 2020

Availability

Academic Institutions and Nonprofits only

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