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pAc5.1B-lambdaN-HA-Dm4E-T_1-58-DmeIF4Ga_687-1666_T

Plasmid

#147580

Purpose

Insect Expression of Dm4E-T_1-58-DmeIF4Ga_687-1666

Depositor

Elisa Izaurralde

Article

Igreja et al Nat Commun. 2014 Sep 2;5:4790. doi: 10.1

Insert

Dm4E-T_1-58-DmeIF4Ga_687-1666 (4E-T Fly)

Expression

Insect

Available Since

May 6, 2022

Availability

Academic Institutions and Nonprofits only

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pJH2028

Plasmid

#179771

Purpose

Pnlf-1::NLF::2XGFP unc-54 3'UTR C.elegans neural expression of NLF::2XGFP

Depositor

Mei Zhen

Article

Xie et al Neuron. 2013 Mar 20;77(6):1069-82. doi:

Insert

NLF-1::2XGFP (nlf-1 Nematode)

Tags

2XGFP

Expression

Worm

Promoter

nlf-1

Available Since

March 14, 2022

Availability

Industry, Academic Institutions, and Nonprofits

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pJH2437

Plasmid

#179780

Purpose

Prgef-1 NLF-1 unc-54 3' UTR C.elegans pan-neural expression of NLF

Depositor

Mei Zhen

Article

Xie et al Neuron. 2013 Mar 20;77(6):1069-82. doi:

Insert

NLF-1 (nlf-1 Nematode)

Tags

no

Expression

Worm

Promoter

Prgef-1

Available Since

March 2, 2022

Availability

Industry, Academic Institutions, and Nonprofits

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pAc5.1B-lambdaN-HA-Dm4E-T_1-510-V5His6_D

Plasmid

#146151

Purpose

Insect Expression of Dm4E-T_1-510

Depositor

Elisa Izaurralde

Article

Igreja et al Nat Commun. 2014 Sep 2;5:4790. doi: 10.1

Insert

Dm4E-T_1-510 (4E-T Fly)

Expression

Insect

Available Since

Feb. 25, 2022

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-EGFP-Dm4E-T_1-510-V5His6_D

Plasmid

#146149

Purpose

Insect Expression of Dm4E-T_1-510

Depositor

Elisa Izaurralde

Article

Igreja et al Nat Commun. 2014 Sep 2;5:4790. doi: 10.1

Insert

Dm4E-T_1-510 (4E-T Fly)

Expression

Insect

Available Since

Feb. 25, 2022

Availability

Academic Institutions and Nonprofits only

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pPPC031

Plasmid

#171152

Purpose

pGNW2 derivative with integration site at P. putida prophage1 for integration of J3-BBa_J23117-mRFP cassette

Depositor

Jesse Zalatan

Article

Kiattisewee et al Metab Eng. 2021 Apr 27. pii: S1096-7176(

Insert

J3-BBa_J23117-mRFP

Expression

Bacterial

Promoter

J3-BBa_J23117

Available Since

Dec. 3, 2021

Availability

Academic Institutions and Nonprofits only

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pPPC034

Plasmid

#171153

Purpose

pGNW2 derivative with integration site at P. putida prophage2 for integration of J3(106)-BBa_J23111-sfGFP cassette

Depositor

Jesse Zalatan

Article

Kiattisewee et al Metab Eng. 2021 Apr 27. pii: S1096-7176(

Insert

J3(106)- BBa_J23111-sfGFP

Expression

Bacterial

Promoter

J3(106)-BBa_J23111

Available Since

Dec. 3, 2021

Availability

Academic Institutions and Nonprofits only

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pBbdCas9S_P0526-sgRNArodA

Plasmid

#149658

Purpose

all-in-one CRISPRi vector for targeting B. burgdorferi rodA

Depositor

Christine Jacobs-Wagner

Article

Takacs et al Appl Environ Microbiol. 2020 Nov 30. pii

Insert

dCas9, lacI, sgRNArodA

Use

CRISPR

Expression

Bacterial

Promoter

PflaB, PpQE30, P0526

Available Since

Sept. 28, 2021

Availability

Academic Institutions and Nonprofits only

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pCDNA3_dPKA-CAT1N17-mVenus

Plasmid

#168497

Purpose

Mammalian expression of the N-terminal 17 residues of Drosophila PKA catalytic subunit 1 C-terminally tagged by monomeric Venus.

Depositor

Haining Zhong

Article

Xiong et al Proc Natl Acad Sci U S A. 2021 Apr 13;11

Insert

N-terminal 17 residues of Drosophila PKA catalytic subunit 1 C-terminally tagged by monomeric Venus

Expression

Mammalian

Available Since

May 11, 2021

Availability

Academic Institutions and Nonprofits only

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pCDNA3_PKA-CβN15-mVenus

Plasmid

#168496

Purpose

Mammalian expression of the N-terminal 15 residues of mouse PKA catalytic subunit β C-terminally tagged by monomeric Venus.

Depositor

Haining Zhong

Article

Xiong et al Proc Natl Acad Sci U S A. 2021 Apr 13;11

Insert

N-terminal 15 residues of mouse PKA catalytic subunit β C-terminally tagged by monomeric Venus

Expression

Mammalian

Available Since

May 11, 2021

Availability

Academic Institutions and Nonprofits only

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pBbdCas9H(-10TC)_arr2

Plasmid

#149587

Purpose

parental, all-in-one CRISPRi vector for B. burgdorferi, parental for gRNA cloning

Depositor

Christine Jacobs-Wagner

Article

Takacs et al Appl Environ Microbiol. 2020 Nov 30. pii

Type

Empty backbone

Use

CRISPR

Expression

Bacterial

Promoter

PflaB, PpQE30

Available Since

Jan. 25, 2021

Availability

Academic Institutions and Nonprofits only

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pBSV2H_Psyn-sgRNAflaB

Plasmid

#149561

Purpose

flaB gRNA expression in B. burgdorferi

Depositor

Christine Jacobs-Wagner

Article

Takacs et al Appl Environ Microbiol. 2020 Nov 30. pii

Insert

sgRNAflaB

Use

CRISPR

Expression

Bacterial

Promoter

Psyn

Available Since

Jan. 7, 2021

Availability

Academic Institutions and Nonprofits only

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pHA847 - mec-4p::hrp-1mScarlet

Plasmid

#139201

Purpose

Expresses mScarlet tagged hrp-1 in mec-4 neurons

Depositor

Anne Hart

Article

Ryan et al EMBO J. 2021 Feb 1;40(3):e105001. doi: 1

Insert

hrp-1 (hrp-1 Nematode)

Expression

Worm

Mutation

mScarlet added between RRMs and LC

Available Since

Dec. 7, 2020

Availability

Academic Institutions and Nonprofits only

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pHA844 - hrp-1p::hrp-1mScarlet

Plasmid

#139204

Purpose

Expresses mScarlet tagged hrp-1

Depositor

Anne Hart

Article

Ryan et al EMBO J. 2021 Feb 1;40(3):e105001. doi: 1

Insert

hrp-1 (hrp-1 Nematode)

Expression

Worm

Mutation

mScarlet added between RRMs and LC

Available Since

Dec. 7, 2020

Availability

Academic Institutions and Nonprofits only

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pJ4M_HRP-1_FL

Plasmid

#139117

Purpose

express HRP-1 with a C-terminal maltose binding protein tag

Depositor

Nicolas Fawzi

Article

Ryan et al EMBO J. 2021 Feb 1;40(3):e105001. doi: 1

Insert

hrp-1 (hrp-1 Nematode)

Expression

Bacterial

Mutation

None

Available Since

Dec. 7, 2020

Availability

Academic Institutions and Nonprofits only

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pBbdCas9S_PflaBS-sgRNAflaB

Plasmid

#149643

Purpose

all-in-one CRISPRi vector for targeting B. burgdorferi flaB

Depositor

Christine Jacobs-Wagner

Article

Takacs et al Appl Environ Microbiol. 2020 Nov 30. pii

Insert

dCas9, lacI, sgRNAflaB

Use

CRISPR

Expression

Bacterial

Promoter

PflaB, PpQE30, PflaBS

Available Since

Dec. 1, 2020

Availability

Academic Institutions and Nonprofits only

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pBbdCas9S(-10TC)_arr2

Plasmid

#149582

Purpose

parental, all-in-one CRISPRi vector for B. burgdorferi, parental for gRNA cloning

Depositor

Christine Jacobs-Wagner

Article

Takacs et al Appl Environ Microbiol. 2020 Nov 30. pii

Type

Empty backbone

Use

CRISPR

Expression

Bacterial

Promoter

PflaB, PpQE30

Available Since

Nov. 30, 2020

Availability

Academic Institutions and Nonprofits only

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pBSV2H_Psyn-sgRNArodA

Plasmid

#149569

Purpose

rodA gRNA expression in B. burgdorferi

Depositor

Christine Jacobs-Wagner

Article

Takacs et al Appl Environ Microbiol. 2020 Nov 30. pii

Insert

sgRNArodA

Use

CRISPR

Expression

Bacterial

Promoter

Psyn

Available Since

Nov. 30, 2020

Availability

Academic Institutions and Nonprofits only

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pBbdCas9S_P0826L-sgRNAflaB

Plasmid

#149648

Purpose

all-in-one CRISPRi vector for targeting B. burgdorferi flaB

Depositor

Christine Jacobs-Wagner

Article

Takacs et al Appl Environ Microbiol. 2020 Nov 30. pii

Insert

dCas9, lacI, sgRNAflaB

Use

CRISPR

Expression

Bacterial

Promoter

PflaB, PpQE30, P0826L

Available Since

Nov. 25, 2020

Availability

Academic Institutions and Nonprofits only

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pBbdCas9S_Psyn-sgRNArodA

Plasmid

#149655

Purpose

all-in-one CRISPRi vector for targeting B. burgdorferi rodA

Depositor

Christine Jacobs-Wagner

Article

Takacs et al Appl Environ Microbiol. 2020 Nov 30. pii

Insert

dCas9, lacI, sgRNArodA

Use

CRISPR

Expression

Bacterial

Promoter

PflaB, PpQE30, Psyn

Available Since

Oct. 26, 2020

Availability

Academic Institutions and Nonprofits only

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