165,947 results

1. SM282_EF1A-dEnAsCas12a-P300-T2A-BlastR

   Plasmid

   #244217

   Purpose

   Mammalian expression of dEnAsCas12a-P300 with Blasticidin resistance selectable marker

   Depositor

   Tim Reddy

   Insert

   dEnAsCas12a-P300
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Mutation

   dEnAsCas12a: E174R, S542R, K548R, and D908A

   Available Since

   Sept. 16, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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2. p2T-CAG-CIBN-eGFP-CAAX-Puro

   Plasmid

   #240804

   Purpose

   Constitutive expression of CIBN-eGFP

   Depositor

   Hans Clevers

   Article

   Krueger et al Science. 2025 Sep 4;389(6764):eadr8753.

   Insert

   CIBN-eGFP
   

   Use

   Synthetic Biology

   Expression

   Mammalian

   Mutation

   none

   Promoter

   CAG

   Available Since

   Sept. 8, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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3. pAAV-GfaABC1D-cOpn5-T2A-EGFP

   Plasmid

   #237859

   Purpose

   The transfer plasmid for packaging AAV expressing chicken OPN5 was constructed by inserting the OPN5 coding sequence into the multiple cloning site under the control of GfaABC1D promoter

   Depositor

   Minmin Luo

   Article

   Dai et al Sci China Life Sci. 2022 Jul;65(7):1271-

   Insert

   Opn5 (OPN5 Chicken)
   

   Use

   AAV

   Promoter

   GfaABC1D

   Available Since

   Aug. 25, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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4. pLV-BFP-Mito

   Plasmid

   #237398

   Purpose

   Lentivirus plasmid for the stable expression of mitochondrial matrix-localized BFP in mammalian cells

   Depositor

   James Hurley

   Article

   Rose et al Proc Natl Acad Sci U S A. 2025 Aug 5;122

   Insert

   COX8A (COX8A Human)
   

   Use

   Lentiviral

   Tags

   EBFP2

   Expression

   Mammalian

   Promoter

   EF1A

   Available Since

   Aug. 1, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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5. pTMN140 - UCOE-SFFV-VPH-XTEN-dCas9-IRES2-BFP-T2A-Blast

   Plasmid

   #238166

   Purpose

   Express VPH-dCas9 and BFP-T2A-BlasticidinR in mammalian cells. Lentiviral

   Depositor

   Thomas Norman

   Article

   Southard et al Nat Genet. 2025 Aug 6. doi: 10.1038/s415

   Insert

   VPH-dCas9
   

   Use

   CRISPR and Lentiviral

   Tags

   mTagBFP2

   Available Since

   June 18, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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6. CFP-LacI

   Plasmid

   #238277

   Purpose

   For overexpression of CFP-LacI

   Depositor

   Denes Hnisz

   Article

   Zhang et al Nature. 2025 Jun 4. doi: 10.1038/s41586-

   Insert

   CFP-LacI
   

   Expression

   Mammalian

   Available Since

   June 18, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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7. Reckleen_2_SpGCas9

   Plasmid

   #233457

   Purpose

   Level 2 RECKLEEN plasmid, genome editing of Klebsiella pneumoniae

   Depositor

   Anke Becker

   Article

   Elsayed et al Commun Biol. 2025 Oct 30;8(1):1509. doi:

   Insert

   Level 2 RECKLEEN plasmid
   

   Use

   CRISPR

   Available Since

   April 24, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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8. pMDC32B-NbmiR482aTS-B/c

   Plasmid

   #227967

   Purpose

   Expression plasmid with a ccdB cassette flanked with two inverted BsaI restriction sites. For expressing syn-tasiRNAs in Nicotiana benthamiana.

   Depositor

   Alberto Carbonell

   Article

   Cisneros et al Nucleic Acids Res. 2025 Feb 27;53(5):gka

   Inserts

   B/c
   NbmiR482aTS
   

   Expression

   Plant

   Promoter

   35S and None

   Available Since

   April 22, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCMV-mGold2t-Lysosome-N-20

   Plasmid

   #231778

   Purpose

   Visualization of lysosome in mammalian cells using mGold2t

   Depositor

   Francois St-Pierre

   Article

   Lee et al Nat Commun. 2025 Apr 4;16(1):3241. doi:

   Insert

   mGold2t-Lysosome-N-20 (Lamp1 Rat)
   

   Tags

   mGold2s

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Feb. 11, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCMK195

    Plasmid

    #226818

    Purpose

    Integrates KanR into NS2, 1250 bp homology, two homologous HIP1 sites in each of the right and left homology arms

    Depositor

    David Nielsen

    Article

    Kamoku et al Biotechnol Prog. 2025 Jan 23:e3518. doi:

    Type

    Empty backbone

    Use

    Unspecified

    Available Since

    Jan. 24, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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11. pET21-pelB-GST-8

    Plasmid

    #228990

    Purpose

    For bacterial expression of anti-GST nanobody GST-8, with pelB leader for periplasmic secretion, and C-term 6xHIS tag.

    Depositor

    Michael Rout

    Article

    Fridy et al J Biol Chem. 2024 Aug 2;300(9):107623. d

    Insert

    GST-8
    

    Tags

    6xHIS

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Jan. 13, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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12. Epac-S-H326

    Plasmid

    #228242

    Purpose

    mT2Del_EPACdDEPCD_Q270E_L777A_K778A_tdBlackcp173Venus(ST) biosensor to measure realtime changes in FRET reflecting fluctuations in cAMP levels in living cells.

    Depositor

    Kees Jalink

    Article

    Zanetti et al FEBS Lett. 2024 Dec 31. doi: 10.1002/187

    Insert

    mT2Del_EPACdDEPCD_Q270E_L777A_K778A_tdBlackcp173Venus(ST) (RAPGEF3 Human)
    

    Expression

    Mammalian

    Mutation

    Deletion of DEP domain, Catalytically dead T781A …

    Promoter

    CMV

    Available Since

    Jan. 7, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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13. Epac-S-H327

    Plasmid

    #228243

    Purpose

    mT2Del_EPACdDEPCD_Q270E_M312L_L777A_K778A_tdBlackcp173Venus(ST) biosensor to measure realtime changes in FRET reflecting fluctuations in cAMP levels in living cells.

    Depositor

    Kees Jalink

    Article

    Zanetti et al FEBS Lett. 2024 Dec 31. doi: 10.1002/187

    Insert

    mT2Del_EPACdDEPCD_Q270E_M312L_L777A_K778A_tdBlackcp173Venus(ST) (RAPGEF3 Human)
    

    Expression

    Mammalian

    Mutation

    Deletion of DEP domain, Catalytically dead T781A …

    Promoter

    CMV

    Available Since

    Jan. 7, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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14. Epac-S-H328

    Plasmid

    #228244

    Purpose

    mT2Del_EPACdDEPCD_Q270E_M312L_E325T_L777A_K778A_tdBlackcp173Venus(ST) biosensor to measure realtime changes in FRET reflecting fluctuations in cAMP levels in living cells.

    Depositor

    Kees Jalink

    Article

    Zanetti et al FEBS Lett. 2024 Dec 31. doi: 10.1002/187

    Insert

    mT2Del_EPACdDEPCD_Q270E_M312L_E325T_L777A_K778A_tdBlackcp173Venus(ST) (RAPGEF3 Human)
    

    Expression

    Mammalian

    Mutation

    Deletion of DEP domain, Catalytically dead T781A …

    Promoter

    CMV

    Available Since

    Jan. 7, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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15. Epac-S-H329

    Plasmid

    #228245

    Purpose

    mT2Del_EPACdDEPCD_Q270E_E325T_L777A_K778A_tdBlackcp173Venus(ST) biosensor to measure realtime changes in FRET reflecting fluctuations in cAMP levels in living cells.

    Depositor

    Kees Jalink

    Article

    Zanetti et al FEBS Lett. 2024 Dec 31. doi: 10.1002/187

    Insert

    mT2Del_EPACdDEPCD_Q270E_E325T_L777A_K778A_tdBlackcp173Venus(ST) (RAPGEF3 Human)
    

    Expression

    Mammalian

    Mutation

    Deletion of DEP domain, Catalytically dead T781A …

    Promoter

    CMV

    Available Since

    Jan. 7, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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16. Epac-S-H332

    Plasmid

    #228250

    Purpose

    mT2Del_EPACdDEPCD_L777A_K778A_tdBlackcp173Venus(ST) biosensor to measure realtime changes in FRET reflecting fluctuations in cAMP levels in living cells.

    Depositor

    Kees Jalink

    Article

    Zanetti et al FEBS Lett. 2024 Dec 31. doi: 10.1002/187

    Insert

    mT2Del_EPACdDEPCD_L777A_K778A_tdBlackcp173Venus(ST) (RAPGEF3 Human)
    

    Expression

    Mammalian

    Mutation

    Deletion of DEP domain, Catalytically dead T781A …

    Promoter

    CMV

    Available Since

    Jan. 7, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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17. pUASTattB-degradALFA

    Plasmid

    #219663

    Purpose

    Permits Gal4-inducible expression of degradALFA, a fusion of anti-ALFANb to the F-Box of Slmb.

    Depositor

    Markus Affolter

    Article

    Aguilar et al Dev Cell. 2024 Oct 7;59(19):2672-2686.e5

    Insert

    degradALFA
    

    Expression

    Insect

    Available Since

    Nov. 13, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. pWN172

    Plasmid

    #215291

    Purpose

    Expresses sfGFP under the Anderson J23113 promoter with an RSF1010 backbone.

    Depositor

    Brian Pfleger

    Article

    Venkataraman et al ACS Synth Biol. 2023 Dec 15;12(12):3623-

    Insert

    sfGFP
    

    Tags

    His

    Expression

    Bacterial

    Promoter

    J23113

    Available Since

    May 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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19. EC70189

    Plasmid

    #209450

    Purpose

    Cloning protospacers for Cas9 editing

    Depositor

    Wendy Harwood, Cristobal Uauy

    Article

    Lawrenson et al Research Square, 12 March 2024, PREPRINT

    Insert

    Cas9 sgRNA
    

    Use

    CRISPR

    Promoter

    HvU3

    Available Since

    Nov. 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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20. pDisplay-GRAB_g5-HT3.0mut

    Plasmid

    #208712

    Purpose

    Expresses the green 5-HT sensor GRAB_g5-HT3.0mut in mammalian cells

    Depositor

    Yulong Li

    Article

    Deng et al Nat Methods. 2024 Apr;21(4):692-702. doi

    Insert

    Green fluorescent 5-HT sensor GRAB_g5-HT3.0mut
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Oct. 13, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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21. p663-UBC-miniTurbo-V5-MAST3_IDG-K

    Plasmid

    #198007

    Purpose

    Gateway destination clone of MAST3 (human) tagged with N-terminal miniTurbo-V5 for generating protein-proximity networks using proximity-dependent biotinylation proteomics

    Depositor

    Ben Major

    Insert

    miniTurbo-V5-MAST3 (MAST3 Human)
    

    Use

    Lentiviral; Gateway destination

    Tags

    miniTurbo-V5

    Expression

    Mammalian

    Promoter

    Ubiquitin

    Available Since

    April 12, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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22. BASIC_SEVA_67_pKD46.10

    Plasmid

    #178942

    Purpose

    BASIC DNA assembly vector

    Depositor

    Paul Freemont

    Article

    Haines et al Synth Biol (Oxf). 2022 Oct 11;7(1):ysac0

    Insert

    mScarlet counter-selection cassette
    

    Use

    Synthetic Biology

    Available Since

    March 1, 2023

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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23. pBS FGF3 isoform 1

    Plasmid

    #19230

    Depositor

    Cliff Tabin

    Article

    Bandyopadhyay et al Dev Biol. 2008 Sep 1;321(1):162-74. doi:

    Insert

    Fibroblast growth factor receptor 3 isoform 1 (FGFR3 Chicken)
    

    Expression

    Bacterial

    Available Since

    Sept. 19, 2008

    Availability

    Academic Institutions and Nonprofits only

24. pcDNA3.1(-) HuTF-Fc-His tag

    Plasmid

    #183253

    Purpose

    Soluble expression of the extracellular domains of human tissue factor (TF)-Fc

    Depositor

    Alexander McLellan

    Insert

    Tissue factor (F3 Human)
    

    Tags

    8xHis

    Expression

    Mammalian

    Available Since

    Aug. 18, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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25. hCathepsin B

    Plasmid

    #11249

    Purpose

    Mammalian expression of human cathepsin B

    Depositor

    Hyeryun Choe

    Article

    Huang et al J Biol Chem. 2006 Feb 10;281(6):3198-203

    Insert

    cathepsin B (CTSB Human)
    

    Tags

    C9 (GTETSQVAPA)

    Expression

    Mammalian

    Available Since

    May 25, 2006

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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26. pcDNA4TO-24xGCN4_v4-kif18b-24xPP7

    Plasmid

    #74928

    Purpose

    Translation reporter

    Depositor

    Marvin Tanenbaum

    Article

    Yan et al Cell. 2016 May 5;165(4):976-89. doi: 10.

    Insert

    24xGCN4_v4-kif18b-24xPP7
    

    Expression

    Mammalian

    Available Since

    May 10, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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27. Lact-C2-GFP

    Plasmid

    #22852

    Depositor

    Sergio Grinstein

    Article

    Yeung et al Science. 2008 Jan 11. 319(5860):210-3.

    Insert

    Lactadherin-C2 Domain (MFGE8 Bovine)
    

    Tags

    GFP

    Expression

    Mammalian

    Available Since

    Jan. 15, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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28. pSNAPf-Cox8A Control Plasmid

    Plasmid

    #101129

    Purpose

    Expression of SNAP-Tagged Cox8A in Mammalian cells

    Depositor

    New England Biolabs, Ana Egana

    Insert

    Cox8A (COX8A Human)
    

    Tags

    SNAP-tag (SNAPf)

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Sept. 27, 2017

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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29. pBS1C

    Plasmid

    #55168

    Purpose

    Empty vector, integration at amyE, ampr, cmr

    Depositor

    Thorsten Mascher

    Article

    Radeck et al J Biol Eng. 2013 Dec 2;7(1):29. doi: 10.

    Type

    Empty backbone

    Use

    Synthetic Biology; Bacillus biobrick box

    Available Since

    Aug. 6, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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30. SpyTag003-Talin rod-mCherry

    Plasmid

    #133567

    Purpose

    Expresses SpyTag003-Talin rod-mCherry in mammalian cells

    Depositor

    Mark Howarth

    Article

    Keeble et al Proc Natl Acad Sci U S A. 2019 Dec 10. p

    Insert

    SpyTag003-Talin rod-mCherry
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Dec. 19, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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