163,606 results

1. AAV pCAG-FLEX-tdTomato-WPRE

   Plasmid

   #51503

   Purpose

   Can be used to generate AAV virus that will express tdTomato in the presence of Cre

   Depositor

   Hongkui Zeng

   Article

   Oh et al Nature. 2014 Apr 10;508(7495):207-14. do

   Insert

   tdTomato
   

   Use

   AAV

   Tags

   Expression

   Mutation

   Promoter

   pCAG

   Available Since

   Aug. 29, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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2. pET-28b-RfxCas13d-His

   Plasmid

   #141322

   Purpose

   Plasmid for bacterial expression and purification of RfxCas13d protein

   Depositor

   Ariel Bazzini, Miguel Angel Moreno-Mateos

   Article

   Kushawah et al Dev Cell. 2020 Sep 28;54(6):805-817.e7.

   Insert

   Rfx-Cas13d
   

   Use

   CRISPR

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available Since

   Aug. 7, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. pFRT_TO_FlagHA_G3BP1

   Plasmid

   #127106

   Depositor

   Thomas Tuschl

   Article

   Meyer et al Mol Cell. 2020 Mar 19;77(6):1193-1205.e5

   Insert

   G3BP1 (G3BP1 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   July 31, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. pLJC5-3XHA-EGFP-PEX26

   Plasmid

   #139054

   Purpose

   HA-PEROXO Lentiviral Construct. Expresses a Peroxisomal Tag.

   Depositor

   David Sabatini

   Article

   Ray et al iScience. 2020 May 22;23(5):101109. doi:

   Insert

   3XHA-EGFP-PEX26
   

   Use

   Lentiviral

   Tags

   HA

   Expression

   Mutation

   Promoter

   UBC

   Available Since

   March 11, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. pHW394 (15xUAS::GFP::let-858 3'UTR)

   Plasmid

   #85584

   Purpose

   cGAL effector (15xUAS::GFP::let-858 3'UTR) construct for C. elegans

   Depositor

   Paul Sternberg

   Article

   Wang et al Nat Methods. 2016 Dec 19. doi: 10.1038/n

   Insert

   15xUAS-delta pes-10-GFP
   

   Use

   Tags

   Expression

   Worm

   Mutation

   GFP(S65C) with three introns for expression in C.…

   Promoter

   15xUAS-delta pes-10-GFP

   Available Since

   Jan. 30, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. pcDNA3.1_V5-TWIST1

   Plasmid

   #215030

   Purpose

   Mammalian expression of human TWIST1

   Depositor

   Joanna Wysocka

   Article

   Kim et al Cell. 2024 Feb 1;187(3):692-711.e26. doi

   Insert

   twist family bHLH transcription factor 1 (TWIST1 Human)
   

   Use

   Tags

   V5

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   March 20, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pAAV-Ef1a-Coff/Fon-mCherry

   Plasmid

   #137134

   Purpose

   Intersectional viral expression of mCherry in cells expressing Flp AND NOT Cre

   Depositor

   Karl Deisseroth, INTRSECT 2.0 Project

   Article

   Fenno et al Neuron. 2020 Jun 20. pii: S0896-6273(20)

   Has Service

   AAV8

   Insert

   Coff/Fon-mCherry
   

   Use

   AAV, Cre/Lox, and Synthetic Biology ; Flp/frt

   Tags

   Expression

   Mutation

   n/a

   Promoter

   EF1a

   Available Since

   June 25, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. pAAV-flex-taCasp3-TEVp

   Plasmid

   #45580

   Depositor

   Jim Wells, Nirao Shah

   Article

   Yang et al Cell. 2013 May 9;153(4):896-909. doi: 10

   Has Service

   AAV1 and AAV5

   Inserts

   Caspase 3 (CASP3 Synthetic, Human)
   Tobacco Etch Virus protease
   

   Use

   AAV; Adeno associated viral vector

   Tags

   Expression

   Mutation

   Linker replaced with a TEV protease cleavage site

   Promoter

   EF1a

   Available Since

   July 10, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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9. pBabe_Zeo_cOVA_T2A_mStrawberry

   Plasmid

   #161736

   Purpose

   Expression of cOVA and mStrawberry

   Depositor

   Alec Kimmelman

   Article

   Yamamoto et al Nature. 2020 May;581(7806):100-105. doi:

   Insert

   cytoplasmic ovalbumin
   

   Use

   Retroviral

   Tags

   mStrawberry

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Dec. 18, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. pGEx-4T-1-YTHDF2

    Plasmid

    #52301

    Purpose

    Encode YTHDF2 isoform 2 with N-terminal GST tag for E. coli expression

    Depositor

    Chuan He

    Article

    Wang et al Nature. 2014 Jan 2;505(7481):117-20. doi

    Insert

    YTHDF2 (YTHDF2 Human)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    Promoter

    tac

    Available Since

    April 14, 2014

    Availability

    Academic Institutions and Nonprofits only

11. pEG BacMam

    Plasmid

    #160451

    Purpose

    vector optimized for use in screening assays, as well as for efficient production of baculovirus and robust expression of the target protein

    Depositor

    Eric Gouaux

    Article

    Goehring et al Nat Protoc. 2014 Nov;9(11):2574-85. doi:

    Type

    Empty backbone

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Dec. 9, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. pEF-GFP

    Plasmid

    #11154

    Purpose

    Mammalian expression vector for expression of GFP (EF1a promoter)

    Depositor

    Connie Cepko

    Article

    Matsuda et al Proc Natl Acad Sci U S A. 2004 Jan 6. 10

    Has Service

    Cloning Grade DNA

    Insert

    EF1 alpha promoter (EEF1A1 Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    May 25, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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13. plRL19

    Plasmid

    #163634

    Purpose

    L5 Integrase delivery plasmid to allow integration of L5 attP only plasmids (e.g. plRL61, ID #163633).

    Depositor

    Jeremy Rock

    Article

    Bosch et al Cell. 2021 Jul 16. pii: S0092-8674(21)00

    Insert

    L5 Integrase
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Mycobacterial Optimized Promoter (MOP) and Tet-On

    Available Since

    Aug. 11, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. 6xHis-MBP-huTsCas12a

    Plasmid

    #174687

    Purpose

    Expresses 6xHis-MBP tagged TsCas12a

    Depositor

    Piyush Jain

    Article

    Nguyen et al medRxiv 2021.07.21

    Insert

    huTsCas12a
    

    Use

    CRISPR and Synthetic Biology

    Tags

    6xHis, MBP, TEV, NLS and NLS, 6xHis, 3xHA

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    Oct. 25, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. mini-pHelper-1.0

    Plasmid

    #230933

    Purpose

    The mini verison (8.4 kb) of Ad helper plasmid to provide E2A, E4orf6, and VA RNA for AAV production

    Depositor

    Qizhao Wang

    Type

    Empty backbone

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Jan. 16, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pSMART Tmem192-3X HA (targeting vector for genomic tagging)

    Plasmid

    #175777

    Purpose

    pSMART Tmem192-3X HA (targeting vector for genomic tagging)

    Depositor

    Wade Harper

    Article

    Eapen et al eLife. 2021 Sep 29;10. pii: 72328. doi:

    Insert

    TMEM192 (TMEM192 Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Feb. 24, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. pEB2-mScarlet

    Plasmid

    #104006

    Purpose

    Plasmid encoding mScarlet

    Depositor

    Philippe Cluzel

    Article

    Balleza et al Nat Methods. 2018 Jan;15(1):47-51. doi:

    Insert

    mScarlet
    

    Use

    Low copy

    Tags

    Expression

    Bacterial

    Mutation

    Mutations relative to DsRed (MSKGE AVIKEF… N6A, R…

    Promoter

    proC

    Available Since

    Dec. 27, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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18. pLKO-Tet-On-shRNA-Control

    Plasmid

    #98398

    Purpose

    Lentivirus for expression of non-targeting control shRNA (Dox-inducible)

    Depositor

    Roland Friedel

    Article

    Junqueira Alves et al Nat Commun. 2021 Oct 14;12(1):6019. doi:

    Insert

    non-targeting randomized sequence
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    July 12, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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19. loxP-G418-LoxP-TurboID-Rab11 HR

    Plasmid

    #230026

    Purpose

    Homology repair plasmid for endogenous tagging of Rab11 at the N-terminus with TurboID and a V5 epitope tag. Contains a G418 resistance cassette for selection of edited cells

    Depositor

    Francesca Bottanelli

    Article

    Adarska et al STAR Protoc. 2025 Aug 4;6(3):103949. doi

    Insert

    genomic sequence Rab11 locus (RAB11A Human)
    

    Use

    CRISPR

    Tags

    Turbo-ID, V5

    Expression

    Mutation

    Promoter

    Available Since

    Jan. 3, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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20. pT7-TaGu-WT-3xF-ORF

    Plasmid

    #238487

    Purpose

    For production of C-term flag-tagged TaGu R2 ORF mRNA for mRNA transfection in PRINT

    Depositor

    Kathleen Collins

    Article

    Zhang et al Nat Biotechnol. 2024 Feb 20. doi: 10.103

    Insert

    R2 retroelement mRNA
    

    Use

    Ivt template

    Tags

    3x-Flag

    Expression

    Mutation

    Promoter

    Available Since

    May 14, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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21. ABE8e-SpRY

    Plasmid

    #226858

    Purpose

    Expresses ABE8e-SpRY base editor in mammalian cells with eGFP marker

    Depositor

    Alexis Komor

    Article

    Osgood et al Methods in Enzymology, February 5, 2025

    Insert

    ABE8e-SpRY
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Feb. 10, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pBad-sfGFP 150TAG

    Plasmid

    #85483

    Purpose

    Arabinose inducible E. coli codon optimized superfolder GFP with TAG stop codon at position 150

    Depositor

    Ryan Mehl

    Article

    Miyake-Stoner et al Biochemistry. 2010 Mar 2;49(8):1667-77.

    Insert

    superfolder GFP 150TAG
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    150TAG (amino acid 150 mutated to stop codon)

    Promoter

    Arabinose

    Available Since

    Jan. 10, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pcDNA3 HA-Sumo2 WT

    Plasmid

    #48967

    Purpose

    Expresses HA SUMO2 Wild Type in mammalian cells

    Depositor

    Guy Salvesen

    Article

    Bekes et al J Biol Chem. 2011 Mar 25;286(12):10238-4

    Insert

    SUMO2 (SUMO2 Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    T7

    Available Since

    Oct. 23, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pcsII-LMP7-flag

    Plasmid

    #86773

    Purpose

    C-terminal flag-tagged protein expression in mammalian cells, lentiviral vector

    Depositor

    Do-Hyung Kim

    Article

    Yun et al Mol Cell. 2016 Feb 18;61(4):625-39. doi:

    Insert

    immunoproteasome beta subunit 5 (LMP7) (PSMB8 Human)
    

    Use

    Lentiviral

    Tags

    flag

    Expression

    Mammalian

    Mutation

    Promoter

    EF-1a

    Available Since

    Jan. 26, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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25. pLenti.CAG.H2B-Dendra2.W

    Plasmid

    #51005

    Purpose

    Lentivector that expresses H2B-Dendra2 from internal CAG enhancer-promoter

    Depositor

    Rusty Lansford

    Insert

    H2B-Dendra2
    

    Use

    Lentiviral

    Tags

    histone2B

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available Since

    June 2, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pCEP4-M2L

    Plasmid

    #20926

    Purpose

    used in the derivation of human iPS cells using non-integrating episomal vectors; expresses cMyc and Lin28

    Depositor

    James Thomson

    Article

    Yu et al Science. 2009 Mar 26.

    Inserts

    MYC (MYC Human)
    LIN28 (LIN28A Human)
    

    Use

    Episomal/ebna

    Tags

    Expression

    Mutation

    Promoter

    CMV and IRES

    Available Since

    May 20, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pAAV-syn-iGluSnFR4f-PDGFR-WPRE

    Plasmid

    #234436

    Purpose

    AAV-mediated expression of glutamate sensor with improved sensitivity and fast deactivation kinetics (4f = fast); NGR vector matches or outperforms PDGFR in all conditions tested.

    Depositor

    GENIE Project

    Article

    Aggarwal et al bioRxiv 2025.03.20.643984

    Insert

    iGluSnFR4f-PDGFR
    

    Use

    AAV

    Tags

    IgK chain and Myc epi tag-PDGFR TM Domain

    Expression

    Mammalian

    Mutation

    Promoter

    Synapsin

    Available Since

    March 20, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pAAV-mDlx-ChR2-mCherry-Fishell-3

    Plasmid

    #83898

    Purpose

    ChR2-mCherry fusion expression in forebrain GABA-ergic interneurons under the control of the mDlx enhancer element

    Depositor

    Gordon Fishell

    Article

    Dimidschstein et al Nat Neurosci. 2016 Oct 31. doi: 10.1038/

    Has Service

    AAV Retrograde, AAV1, and AAV9

    Insert

    ChR2
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    mDlx

    Available Since

    Oct. 31, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. hCas9

    Plasmid

    #41815

    Purpose

    Expresses human codon optimized Cas9 nuclease for genome engineering

    Depositor

    George Church

    Article

    Mali et al Science. 2013 Feb 15;339(6121):823-6. do

    Insert

    Cas9
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    human codon-optimized

    Promoter

    CMV

    Available Since

    Jan. 11, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. MYC_pLX307

    Plasmid

    #98363

    Purpose

    Lentiviral expression of MYC

    Depositor

    Sefi Rosenbluh, William Hahn

    Article

    Rosenbluh et al Cell Syst. 2016 Sep 28;3(3):302-316.e4.

    Insert

    MYC (MYC Human)
    

    Use

    Lentiviral

    Tags

    NA

    Expression

    Mammalian

    Mutation

    Promoter

    E1Fa

    Available Since

    Aug. 23, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge