161,273 results

1. pCMV-PEmax-P2A-hP53DD-P2A-hMLH1dn

   Plasmid

   #214102

   Purpose

   Mammalian expression of SpCas9 PEmax prime editor with P2A-human P53DD and P2A-human MLH1dn

   Depositor

   Ting Zhou

   Article

   Wu et al Nat Commun. 2024 Dec 30;15(1):10824. doi

   Insert

   PE-Plus
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   June 6, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCS4083

   Plasmid

   #131739

   Purpose

   Two Color Control Assay bicistronic BFP-mCherry-sTRSVctl

   Depositor

   Christina Smolke

   Article

   Xiang et al Nat Commun. 2019 Sep 23;10(1):4327. doi:

   Insert

   sTRSV
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   June 9, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. AttB _EGFP

   Plasmid

   #171597

   Purpose

   Strong EGFP fluorescence from Bxb1 landing pad

   Depositor

   Kenneth Matreyek

   Article

   Shukla et al PLoS Pathog. 2021 Jul 16;17(7):e1009715.

   Insert

   EGFP
   

   Use

   Synthetic Biology; Promoterless bxb1 dna recombin…

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   July 21, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pSBBi-Pur GFP-TM

   Plasmid

   #139185

   Purpose

   SB-transposon with constitutive bi-directional promoter. One side contains transmembrane-anchored GFP; the other side contains the puromycin resistance gene.

   Depositor

   Neal Devaraj

   Insert

   GFP-TM
   

   Use

   Transposon

   Tags

   Expression

   Mammalian

   Mutation

   fused to membrane anchor

   Promoter

   EF1α

   Available since

   March 27, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pDONR221-Sen1

   Plasmid

   #99994

   Purpose

   Shuttle yeast Sen1 into Gateway destination vector

   Depositor

   Susan Baserga

   Article

   Vincent et al RNA. 2017 Oct 20. pii: rna.062927.117. d

   Insert

   Sen1 (SEN1 Budding Yeast)
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   Aug. 25, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. pGWT35S

   Plasmid

   #182790

   Purpose

   Destination vector for transient expression in plant

   Depositor

   Yutaka Kodama

   Article

   Fujii et al Biotechniques. 2018 Apr;64(4):153-161. d

   Type

   Empty backbone

   Use

   Tags

   Expression

   Plant

   Mutation

   Promoter

   Available since

   June 9, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. pKC518 pBABE EGFP-LIN52

   Plasmid

   #192697

   Purpose

   Retroviral transgene expression of human LIN52

   Depositor

   Iain Cheeseman

   Article

   Funk et al Cell. 2022 Nov 23;185(24):4634-4653.e22.

   Insert

   LIN52 (LIN52 Human)
   

   Use

   Retroviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Oct. 31, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCR-U6-gRNA-miniCMV-TdTomato

   Plasmid

   #192656

   Purpose

   A plasmid encoding miniCMV sgRNA and miniCMV-promoter-TdTomato

   Depositor

   Jeffrey Miner

   Article

   Omachi et al PLoS One. 2022 Jun 28;17(6):e0270008. do

   Insert

   TdTomato
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6, miniCMV

   Available since

   Dec. 15, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pDB-His-MBP-SARM1

   Plasmid

   #195762

   Purpose

   Purification of SARM1 from E.coli

   Depositor

   Hao Wu

   Insert

   SARM1 (SARM1 Human)
   

   Use

   Tags

   HIS-MBP-TEV

   Expression

   Bacterial

   Mutation

   truncation of first 19 residues

   Promoter

   T7

   Available since

   Feb. 16, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. 4xCSL-luciferase

    Plasmid

    #41726

    Depositor

    Raphael Kopan

    Article

    Saxena et al J Biol Chem. 2001 Oct 26;276(43):40268-7

    Insert

    4xCSL binding sites
    

    Use

    Luciferase

    Tags

    Firefly luciferase

    Expression

    Mammalian

    Mutation

    Contains a multimerized high affinity CSL binding…

    Promoter

    Available since

    Dec. 13, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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11. 5aa SunTag VP64 CLV3

    Plasmid

    #115485

    Purpose

    CRISPR-Cas9 SunTag system to target VP64 to the CLV3 locus with two guide RNAs

    Depositor

    Steven Jacobsen

    Article

    Papikian et al Nat Commun. 2019 Feb 13;10(1):729. doi:

    Insert

    g2_U6_g1_U6_NOS_NLS_GB1_NLS_linker_VP64_linker_sfGFP_scFv_UBQ10_Insulator_UBQ10_Ω_dCas9_1xHA_2xNLS_linker_10xGCN4_OCS
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    March 21, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. pTHSSd_34

    Plasmid

    #59960

    Purpose

    PTac GFP expression

    Depositor

    Christopher Voigt

    Article

    Segall-Shapiro et al Mol Syst Biol. 2014 Jul 30;10(7):742.

    Insert

    sfGFP
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    N/A

    Promoter

    PTac

    Available since

    Sept. 17, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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13. pAV hH1-ARRET

    Plasmid

    #111374

    Purpose

    ARRET expression contains approximately 130 anti-sense telomere hexanucleotide repeats of TTAGGG and TTGGGG variant sequences

    Depositor

    Klaus Holzmann

    Insert

    ARRET w/o polyA
    

    Use

    Adenoviral

    Tags

    Expression

    Mutation

    Promoter

    hH1

    Available since

    Sept. 25, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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14. pAV10.F3

    Plasmid

    #63199

    Purpose

    BioBrick pSB1C3 backbone (Cm), HIS3, RFP flanked by BsaI sites with CAGT & TTTT overhangs for transcription unit (TU) assembly using yGG. Integration into chrVI: 97873-98803 (NotI or BciVI).

    Depositor

    Jef Boeke

    Article

    Agmon et al ACS Synth Biol. 2015 Jul 17;4(7):853-9.

    Type

    Empty backbone

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial and Yeast

    Mutation

    Promoter

    Available since

    Nov. 28, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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15. PLKO.1-SMG6-CDS-1

    Plasmid

    #136046

    Purpose

    SMG6 shRNA (Targeting CDS #1) inserted into the PLKO.1 plasmid (AACTTGTAAGTAACCTGCAGC)

    Depositor

    Anthony Leung

    Article

    Fischer et al Mol Cell. 2020 Apr 2;78(1):70-84.e6. doi

    Insert

    SMG6 (SMG6 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 4, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. PLKO.1-SMG6-CDS-2

    Plasmid

    #136047

    Purpose

    SMG6 shRNA (Targeting CDS #2) inserted into the PLKO.1 plasmid (AAGGAGTTCCAGGTGTTACTG)

    Depositor

    Anthony Leung

    Article

    Fischer et al Mol Cell. 2020 Apr 2;78(1):70-84.e6. doi

    Insert

    SMG6 (SMG6 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 4, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. pHR-scFv-GCN4-sfGFP-GB1-NLS-dWPRE

    Plasmid

    #60906

    Purpose

    The plasmid encodes an antibody that binds to the GCN4 peptide from the SunTag system, and is fused to sfGFP and a NLS for imaging

    Depositor

    Ron Vale

    Article

    Tanenbaum et al Cell. 2014 Oct 8. pii: S0092-8674(14)012

    Insert

    scFv-GCN4
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 17, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. 5aa SunTag VP64 AP3

    Plasmid

    #115484

    Purpose

    CRISPR-Cas9 SunTag system to target VP64 to the AP3 locus with two guide RNAs

    Depositor

    Steven Jacobsen

    Article

    Papikian et al Nat Commun. 2019 Feb 13;10(1):729. doi:

    Insert

    g2_U6_g1_U6_NOS_NLS_GB1_NLS_linker_VP64_linker_sfGFP_scFv_UBQ10_Insulator_UBQ10_Ω_dCas9_1xHA_2xNLS_linker_10xGCN4_OCS
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    March 21, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. KSR1-NanoLuc Fusion Vector

    Plasmid

    #237175

    Purpose

    Express KSR1-NanoLuc(R) Fusion Protein in Mammalian Cells under a CMV promoter

    Depositor

    Promega Corporation

    Has Service

    DNA

    Insert

    KSR1 (KSR1 Human)
    

    Use

    Tags

    NanoLuc (R)

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 14, 2025

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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20. NanoBRET BiBRET Vector HaloTag-HRAS WT-BRAF RBD-NanoLuc

    Plasmid

    #236850

    Purpose

    Express HaloTag(R)-HRAS WT-BRAF RBD-NanoLuc(R) in Mammalian Cells under a CMV promoter

    Depositor

    Promega Corporation

    Has Service

    DNA

    Inserts

    HRAS (HRAS Human)
    BRAF (RBD) (BRAF Human)
    

    Use

    Tags

    HaloTag (R) and NanoLuc (R)

    Expression

    Mammalian

    Mutation

    None

    Promoter

    CMV

    Available since

    July 14, 2025

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

21. GLP-1R-SmBiT Fusion Vector

    Plasmid

    #236845

    Purpose

    Express GLP-1R-SmBiT in Mammalian Cells under a CMV promoter

    Depositor

    Promega Corporation

    Has Service

    DNA

    Insert

    GLP-1R (GLP1R Human)
    

    Use

    Tags

    SmBiT

    Expression

    Mammalian

    Mutation

    None

    Promoter

    CMV

    Available since

    July 14, 2025

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

22. KSR2-HaloTag Fusion Vector

    Plasmid

    #236793

    Purpose

    Express KSR2-HaloTag(R) Fusion Protein in Mammalian Cells under a CMV promoter

    Depositor

    Promega Corporation

    Has Service

    DNA

    Insert

    KSR2 (KSR2 Human)
    

    Use

    Tags

    HaloTag (R)

    Expression

    Mammalian

    Mutation

    None

    Promoter

    CMV

    Available since

    July 14, 2025

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

23. KSR1-HaloTag Fusion Vector

    Plasmid

    #236794

    Purpose

    Express KSR1-HaloTag(R) Fusion Protein in Mammalian Cells under a CMV promoter

    Depositor

    Promega Corporation

    Has Service

    DNA

    Insert

    KSR1 (KSR1 Human)
    

    Use

    Tags

    HaloTag (R)

    Expression

    Mammalian

    Mutation

    None

    Promoter

    CMV

    Available since

    July 14, 2025

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

24. pIDTsmart-RC2-delVP1-VP1-588TAG

    Plasmid

    #240122

    Purpose

    Express AAV2 capsid with ncAA mutant at site 588 of VP1

    Depositor

    Abhishek Chatterjee

    Article

    Pham et al bioRxiv 2024.07.20.604406

    Inserts

    RC2-DelVP1
    VP1-588-TAG
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 11, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. Cyclin B1-Venus ND

    Plasmid

    #234673

    Purpose

    expresses a non-degradable cyclin B1 in fusion with Venus

    Depositor

    Lienhard Schmitz

    Article

    Seibert et al J Cell Biol. 2019 Apr 1;218(4):1164-1181

    Inserts

    Cyclin B1 (CCNB1 Human)
    Cyclin B1 (CCNB1 Human)
    

    Use

    Tags

    Venus

    Expression

    Mammalian

    Mutation

    double mutation R42A and L45A

    Promoter

    CMV

    Available since

    June 5, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pRCA892 - TFORF Empty Vector Puro-T2A-GFP (pRCA886 backbone)

    Plasmid

    #238180

    Purpose

    Lentiviral empty vector

    Depositor

    Thomas Norman

    Article

    Southard et al bioRxiv [Preprint]. 2024 Aug 3:2024.07.3

    Type

    Empty backbone

    Use

    Lentiviral

    Tags

    GFP

    Expression

    Mutation

    Promoter

    Available since

    June 3, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. makeTCR_Lentiviral_Backbone

    Plasmid

    #233943

    Purpose

    backbone for makeTCR assembly

    Depositor

    Michael Platten

    Type

    Empty backbone

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    EF1a (long)

    Available since

    April 24, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pQE-meGFP-DLC1

    Plasmid

    #192048

    Purpose

    Plasmid expresses DLC1 binding peptide to Talin R8 domain in chimera with meGFP

    Depositor

    Ionel Popa

    Article

    Dahal et al Sci Adv. 2022 Jul 15;8(28):eabl7719. doi

    Insert

    DLC1 (DLC1 Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Feb. 21, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pHAGE-RFP-SKL

    Plasmid

    #183726

    Purpose

    For expression of RFP targeted to peroxisomes

    Depositor

    Jeffrey Gerst

    Article

    Haimovich et al bioRxiv 2024.11.06.622258

    Insert

    mRFP1
    

    Use

    Lentiviral

    Tags

    SKL (peroxisomal targeting sequence 1)

    Expression

    Mutation

    Promoter

    UbiC

    Available since

    Nov. 11, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. SpCas9-NG-APOBEC-nCas9-Ung

    Plasmid

    #198539

    Purpose

    Expresses human codon-optimized SpCas9-NG-APOBEC-nCas9-Ung and blasticidin resistance: EFS promoter-SpCas9-NG-APOBEC-nCas9-Ung-NLS-FLAG-P2A-BSD

    Depositor

    Hyongbum Kim

    Article

    Kim et al Nat Biotechnol. 2024 Mar;42(3):484-497.

    Insert

    SpCas9-NG-APOBEC-nCas9-Ung
    

    Use

    CRISPR and Lentiviral

    Tags

    BSD, FLAG, and NLS

    Expression

    Mammalian

    Mutation

    Promoter

    EFS

    Available since

    Nov. 5, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge