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pGX-5Dual

Plasmid

#226869

Purpose

transient expression

Depositor

Guoyong Xu

Article

Niu et al Database (Oxford). 2020 Jan 1;2020:baaa0

Insert

Luciferase and hRluc

Expression

Plant

Available Since

Dec. 5, 2024

Availability

Academic Institutions and Nonprofits only

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LentiCRISPRv2-Opti

Plasmid

#163126

Purpose

LentiCRISPRv2 variant with modified sgRNA scaffold from Chen et al. 2013 Cell.

Depositor

David Sabatini

Article

Adelmann et al Nature. 2020 Nov 18. pii: 10.1038/s41586

Type

Empty backbone

Use

CRISPR and Lentiviral

Available Since

Feb. 16, 2021

Availability

Academic Institutions and Nonprofits only

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pCMV4-BlaM-Vpr

Plasmid

#21950

Depositor

Warner Greene

Article

Cavrois et al Nat Biotechnol. 2002 Nov . 20(11):1151-4

Insert

BlaM-Vpr (vpr Fusion of BlaM and HIV-1 Vpr)

Expression

Mammalian

Available Since

Oct. 1, 2009

Availability

Academic Institutions and Nonprofits only
pCMV-PTEN

Plasmid

#28298

Depositor

Maria-Magdalena Georgescu

Article

Takahashi et al EMBO J. 2006 Feb 22. 25(4):910-20.

Insert

PTEN (PTEN Human)

Tags

FLAG

Expression

Mammalian

Available Since

Sept. 14, 2011

Availability

Academic Institutions and Nonprofits only

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ER-mRFP

Plasmid

#62236

Purpose

Plasmid expresses endoplasmic reticulum targeted mRFP in mammalian cells.

Depositor

Erik Snapp

Article

Snapp et al Proc Natl Acad Sci U S A. 2006 Apr 25;10

Insert

ER-mRFP

Tags

mRFP1

Expression

Mammalian

Promoter

cmv

Available Since

March 25, 2015

Availability

Academic Institutions and Nonprofits only

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Zhi3-mCherry

Plasmid

#106295

Purpose

CMV driven mCherry, zeocin resistance

Depositor

Sanford Simon

Article

Belote et al J Cell Biol. 2020 Jan 6;219(1). pii: 132

Insert

mCherry

Use

Lentiviral

Expression

Mammalian

Promoter

CMV

Available Since

March 22, 2022

Availability

Academic Institutions and Nonprofits only

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pHPRT-DRGFP

Plasmid

#26476

Purpose

DR-GFP substrate (Addgene 26475) flanked by mouse hprt targeting sequences

Depositor

Maria Jasin

Article

Pierce et al Genes Dev. 2001 Dec 15. 15(24):3237-42.

Insert

pHPRT-DRGFP

Use

Mouse Targeting

Expression

Mammalian

Available Since

Nov. 3, 2010

Availability

Academic Institutions and Nonprofits only

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pYPQ131A

Plasmid

#69273

Purpose

Golden Gate entry vector; 1st gRNA under AtU6 promoter

Depositor

Yiping Qi

Article

Lowder et al Plant Physiol. 2015 Aug 21. pii: pp.0063

Type

Empty backbone

Use

CRISPR

Available Since

Sept. 18, 2015

Availability

Academic Institutions and Nonprofits only

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pJPC259

Plasmid

#234531

Purpose

Cre/Lox 71/66 Recombinase switch; plant T-DNA

Depositor

Daniel Voytas

Article

Chamness et al Plant Physiol. 2025 Mar 1;197(3):kiaf073

Insert

RUBY

Expression

Plant

Mutation

none

Available Since

April 18, 2025

Availability

Academic Institutions and Nonprofits only

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Src-EGFP

Plasmid

#110496

Purpose

Fluorescent Src

Depositor

Terence Dermody, Bernardo Mainou

Article

Mainou et al J Virol. 2011 Apr;85(7):3203-13. doi: 10

Insert

c-Src (Src Mouse)

Tags

EGFP

Expression

Mammalian

Available Since

May 15, 2018

Availability

Academic Institutions and Nonprofits only

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pRF-TetR

Plasmid

#49374

Depositor

Christopher Voigt

Article

Stanton et al Nat Chem Biol. 2013 Dec 8. doi: 10.1038/

Insert

TetR repressor

Use

Synthetic Biology

Expression

Bacterial

Available Since

Aug. 25, 2015

Availability

Academic Institutions and Nonprofits only

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GFP-N-WASP

Plasmid

#47406

Depositor

Peter McPherson

Article

Hussain et al Nat Cell Biol. 2001 Oct;3(10):927-32.

Insert

N-WASP

Tags

EGFP

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 9, 2018

Availability

Academic Institutions and Nonprofits only

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ZE32 Prickle-1-GFP-pCS2+

Plasmid

#17073

Depositor

Randall Moon

Article

Veeman et al Curr Biol. 2003 Apr 15. 13(8):680-5.

Insert

Prickle-1 (prickle1a Zebrafish)

Use

Zebrafish expression

Tags

GFP

Available Since

Feb. 1, 2008

Availability

Academic Institutions and Nonprofits only
pJPC430

Plasmid

#234529

Purpose

CinH Recombinase switch; plant T-DNA

Depositor

Daniel Voytas

Article

Chamness et al Plant Physiol. 2025 Mar 1;197(3):kiaf073

Insert

RUBY

Expression

Plant

Mutation

none

Available Since

April 18, 2025

Availability

Academic Institutions and Nonprofits only

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CAG-rtTA

Plasmid

#185562

Purpose

Ubiquitous expression of reverse tetracycline transactivator for TetON system

Depositor

Connie Cepko

Article

Amamoto et al Elife. 2022 Mar 22;11. pii: 76389. doi:

Insert

rtTA

Expression

Mammalian

Promoter

CAG

Available Since

June 23, 2022

Availability

Academic Institutions and Nonprofits only

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FIRES-PBCS-CLV3

Plasmid

#228697

Purpose

Sensor cassette FIRE1, FIRE fluorescent ratiometric sensor

Depositor

Carolyn Sangokoya

Article

Sangokoya et al Cell Rep Methods. 2025 Jan 11:100960. do

Insert

FIRE1 SENSOR

Use

Synthetic Biology and Unspecified

Tags

mClover3

Expression

Mammalian

Promoter

hpgk

Available Since

Jan. 28, 2025

Availability

Academic Institutions and Nonprofits only

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FIREC-PBCS-MC2

Plasmid

#228698

Purpose

Control cassette FIRE1, FIRE fluorescent ratiometric sensor

Depositor

Carolyn Sangokoya

Article

Sangokoya et al Cell Rep Methods. 2025 Jan 11:100960. do

Insert

FIRE1 CONTROL

Use

Synthetic Biology and Unspecified

Tags

mCherry

Expression

Mammalian

Promoter

EF1-alpha

Available Since

Jan. 28, 2025

Availability

Academic Institutions and Nonprofits only

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mCherry-GFP1-10

Plasmid

#78591

Purpose

Express mCherry-GFP1-10 in mammalian cells

Depositor

Yihong Ye

Article

Lee et al Nat Cell Biol. 2016 Jul;18(7):765-76. do

Insert

GFP 1-10

Tags

mCherry2

Expression

Mammalian

Promoter

CMV

Available Since

June 9, 2016

Availability

Academic Institutions and Nonprofits only

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pJPC263

Plasmid

#234530

Purpose

Cre/Lox P1 Recombinase switch; plant T-DNA

Depositor

Daniel Voytas

Article

Chamness et al Plant Physiol. 2025 Mar 1;197(3):kiaf073

Insert

RUBY

Expression

Plant

Mutation

none

Available Since

April 18, 2025

Availability

Academic Institutions and Nonprofits only

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pYPQ132B

Plasmid

#69282

Purpose

Golden Gate entry vector; 2nd gRNA under AtU3 promoter

Depositor

Yiping Qi

Article

Lowder et al Plant Physiol. 2015 Aug 21. pii: pp.0063

Type

Empty backbone

Use

CRISPR

Available Since

Sept. 14, 2015

Availability

Academic Institutions and Nonprofits only

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Slug_pGL2

Plasmid

#31695

Depositor

Paul Wade

Article

Fujita et al Cell. 2003 Apr 18. 113(2):207-19.

Insert

Slug (SNAI2 Human)

Use

Luciferase

Expression

Mammalian

Available Since

Aug. 29, 2011

Availability

Academic Institutions and Nonprofits only

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pUBQ10-YFP-GW

Plasmid

#209362

Purpose

Gateway cloning compatible binary vector for agrobacterium mediated transformation. Arabidopsis UBQ10 promoter driving expression of N-terminal YFP fusion protein.

Depositor

Lucia Strader

Article

Michniewicz et al BMC Res Notes. 2015 Mar 3;8:63. doi: 10.

Type

Empty backbone

Expression

Plant

Available Since

Jan. 30, 2024

Availability

Academic Institutions and Nonprofits only

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TOP-H2B-YFP-DD

Plasmid

#96891

Purpose

Reporter to monitor translation of 5'TOP mRNA

Depositor

Tobias Meyer

Article

Han et al Nat Methods. 2014 Jan;11(1):86-93. doi:

Insert

TOP-H2B-YFP-DD

Use

Lentiviral

Expression

Mammalian

Available Since

Aug. 9, 2017

Availability

Academic Institutions and Nonprofits only

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Flag-RapGAP

Plasmid

#118324

Purpose

Expression of RapGAP

Depositor

Philip Stork

Article

Liu et al Mol Cell Biol. 2010 Aug;30(16):3956-69.

Insert

RapGAP

Tags

Flag

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 13, 2018

Availability

Academic Institutions and Nonprofits only

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TOP-H2B-mCherry-DD

Plasmid

#96894

Purpose

Reporter to monitor translation of 5'TOP mRNA

Depositor

Tobias Meyer

Article

Han et al Nat Methods. 2014 Jan;11(1):86-93. doi:

Insert

TOP-H2B-mCherry-DD

Use

Lentiviral

Expression

Mammalian

Available Since

Aug. 9, 2017

Availability

Academic Institutions and Nonprofits only

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PB-U6

Plasmid

#120358

Purpose

piggyBac transposon vector for sgRNA expression.

Depositor

Knut Woltjen

Article

Kagawa et al Stem Cell Reports. 2018 Dec 28. pii: S22

Type

Empty backbone

Use

CRISPR; Piggybac transposon

Expression

Mammalian

Promoter

U6

Available Since

April 8, 2021

Availability

Academic Institutions and Nonprofits only

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pCAG1.1

Plasmid

#173685

Purpose

The expression vector for genes of interest under CAG promoter.

Depositor

Masahito Ikawa

Article

Ikawa et al J Biol Chem. 2011 Feb 18;286(7):5639-46.

Type

Empty backbone

Expression

Mammalian

Promoter

CAG promoter

Available Since

Sept. 10, 2021

Availability

Academic Institutions and Nonprofits only

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pCMK195

Plasmid

#226818

Purpose

Integrates KanR into NS2, 1250 bp homology, two homologous HIP1 sites in each of the right and left homology arms

Depositor

David Nielsen

Article

Kamoku et al Biotechnol Prog. 2025 Jan 23:e3518. doi:

Type

Empty backbone

Use

Unspecified

Available Since

Jan. 24, 2025

Availability

Academic Institutions and Nonprofits only

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pcDNA-deadMalibu-mScarlet

Plasmid

#229555

Purpose

Fluorescent reporter for malonyl-CoA that does not bind malonyl-CoA (control)

Depositor

Danielle Schmitt

Article

Ranzau et al bioRxiv [Preprint]. 2024 Sep 28:2024.09.

Insert

Malibu-mScarlet

Tags

mScarlet

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 3, 2025

Availability

Academic Institutions and Nonprofits only

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pET-6xHis-sfGFP

Plasmid

#199171

Purpose

used to express a superfolder GFP in E. coli for comparison to supercharged variants

Depositor

Allie Obermeyer

Article

Yeong et al ACS Cent Sci. 2020 Dec 23;6(12):2301-231

Insert

superfolder GFP

Tags

MGHHHHHHGGA

Promoter

T7

Available Since

May 10, 2023

Availability

Academic Institutions and Nonprofits only

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