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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 26,905 results for: STI

Showing: 5081 - 5120 of 26905 results

  2. APRT

    Plasmid
    #64028
    Purpose
    Expression of S. crervisiae strain APRTase in E. coli. Contains N-terminal HistidineX6 tag.
    Depositor
    Vern Schramm
    Article
    Shi et al Biochemistry. 2001 Sep 11;40(36):10800-9
    Insert
    adenine phosphoribosyltransferase (APT1 Budding Yeast)
    Tags
    Histidine x 6
    Expression
    Bacterial
    Available Since
    April 29, 2015
    Availability
    Academic Institutions and Nonprofits only

  3. pJYDNp

    Plasmid
    #162451
    Purpose
    Positive control plasmid carrying the eUnaG2 bilirubin dependent fluorescent protein fused to the C-terminus of Aga2p protein. This plasmid serves as an expression control.
    Depositor
    Gideon Schreiber
    Article
    Zahradník et al bioRxiv Preprint 2020
    Insert
    Aga2p-eUnaG2
    Use
    Shuttle vector bacteria/yeast
    Tags
    HA and myc
    Promoter
    GAL1
    Available Since
    Feb. 19, 2021
    Availability
    Academic Institutions and Nonprofits only
    162451-plasmid-map-sequence-id-321446

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  4. pFGL815

    Plasmid
    #52322
    Purpose
    A vector backbone for ATMT (Agrobacterium tumerfaciens-mediated transformation). There is a 69-bp MCS (multiple cloning site) which contains 12 unique restriction sites.
    Depositor
    Naweed Naqvi
    Article
    Yang et al Fungal Genet Biol. 2014 Apr 24. pii: S10
    Type
    Empty backbone
    Use
    Agrobacterium
    Expression
    Bacterial
    Available Since
    July 8, 2014
    Availability
    Academic Institutions and Nonprofits only
    52322-plasmid-map-sequence-id-81025

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  7. pEZY19

    Plasmid
    #18668
    Purpose
    pET19b converted Gateway Destination vector for expressing His tagged fusion proteins in bacteria. See note in comments section below regarding the mutated enterokinase site.
    Depositor
    Yu-Zhu Zhang
    Article
    Guo et al Biotechnol J. 2008 Mar . 3(3):370-7.
    Type
    Empty backbone
    Use
    Gateway destination vector
    Tags
    His
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    July 26, 2008
    Availability
    Academic Institutions and Nonprofits only
    18668-plasmid-map-sequence-id-8812

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  15. pET28a-HIS-TagGFP2-TEV-GGG-ELP(120nm)-Cys

    Plasmid
    #90473
    Purpose
    Elastin Like Polypeptide: [(VPGEG)-(VPGVG)4-(VPGAG)2-(VPGGG)2-(VPGEG)]6, with N-Term TagGFP2, and a TEV-cleavage site, leaving an N-terminal Sortase Recognition Sequence GGG and a C-terminal Cysteine
    Depositor
    Hermann Gaub
    Insert
    TagGFP2-TEV-GGG-ELP(120nm)-Cys
    Tags
    Cysteine and Sortase-Tag
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    Aug. 21, 2017
    Availability
    Academic Institutions and Nonprofits only
    90473-plasmid-map-sequence-id-191118

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  16. pJYDC3

    Plasmid
    #162460
    Purpose
    Yeast display plasmid for expression of C-terminal fusions with Aga2p yeast agglutinin. The reporter DnbALFA was fused to the N-terminus of Aga2p.
    Depositor
    Gideon Schreiber
    Article
    Zahradník et al bioRxiv Preprint 2020
    Type
    Empty backbone
    Use
    Shuttle vector bacteria/yeast
    Tags
    DnbALFA designed ALFA nanobody, HA tag, HDEL sequ…
    Available Since
    April 9, 2021
    Availability
    Academic Institutions and Nonprofits only
    162460-plasmid-map-sequence-id-330057

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  17. p663-UBC-2xV5-TurboID-NES-STOP_IDG-K

    Plasmid
    #158162
    Purpose
    Gateway destination clone of TurboID-NES (as control) tagged with N-terminal V5 for generating protein-proximity networks using proximity-dependent biotinylation proteomics
    Depositor
    Ben Major
    Insert
    2xV5-TurboID-NES
    Use
    Lentiviral; Gateway destination
    Tags
    V5
    Expression
    Mammalian
    Promoter
    Ubiquitin
    Available Since
    Oct. 16, 2020
    Availability
    Academic Institutions and Nonprofits only
    158162-plasmid-map-sequence-id-314581

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  25. CD55-full length

    Plasmid
    #220874
    Purpose
    Expresses CD55 variant in mammalian cells
    Depositor
    Ferenc Scheeren
    Insert
    CD55 (CD55 Human)
    Tags
    EGFP
    Expression
    Mammalian
    Promoter
    CMW
    Available Since
    Aug. 23, 2024
    Availability
    Academic Institutions and Nonprofits only
    220874-plasmid-map-sequence-id-443039

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  39. pJYDC2

    Plasmid
    #162459
    Purpose
    Yeast display plasmid for expression of C-terminal fusions. Modification of pJYDC1 vector with ALFA tag in MCS.
    Depositor
    Gideon Schreiber
    Article
    Zahradník et al bioRxiv Preprint 2020
    Type
    Empty backbone
    Use
    Shuttle vector bacteria/yeast
    Tags
    ALFA-tag, HA tag, HDEL sequence, and eUnaG2 fluor…
    Available Since
    Feb. 19, 2021
    Availability
    Academic Institutions and Nonprofits only
    162459-plasmid-map-sequence-id-321477

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Showing: 5081 - 5120 of 26905 results