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HA-Rab8a-CA (Q67L)

Plasmid

#101050

Purpose

Expresses HA-tagged human Rab8-CA (Q67L) in mammalian cells

Depositor

Ken-Ichi Takemaru

Article

Burke et al J Cell Biol. 2014 Oct 13;207(1):123-37.

Insert

Rab8a (RAB8A Human)

Use

Tags

HA

Expression

Mammalian

Mutation

Q67L

Promoter

CMV

Available since

Sept. 20, 2017

Availability

Academic Institutions and Nonprofits only

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U6-DNMT1-hSynapsin-PE2-Nterminal-P2A-EGFP-KASH-lenti

Plasmid

#135955

Purpose

Neuronal expression of N-terminal intein-split prime editor v2 (PE2) with EGFP-KASH marker and U6-mDNMT1 cassette. This plasmid is used by PE2, PE3, and PE3b

Depositor

David Liu

Article

Anzalone et al Nature. 2019 Oct 21. pii: 10.1038/s41586

Insert

Prime editor v2 (PE2) N-terminal

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

hSynapsin

Available since

Jan. 21, 2020

Availability

Academic Institutions and Nonprofits only

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pBABE puro SDF-1 alpha

Plasmid

#12270

Depositor

Bob Weinberg

Article

Orimo et al Cell. 2005 May 6. 121(3):335-48.

Insert

SDF-1 alpha (CXCL12 Human)

Use

Retroviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

July 20, 2006

Availability

Academic Institutions and Nonprofits only

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155_KRAS in pmiRGlo

Plasmid

#78132

Purpose

luciferase reporter for miRNA activity

Depositor

Heidi Schwarzenbach

Article

Eichelser et al Oncotarget. 2014 Oct 30;5(20):9650-63.

Insert

KRAS (KRAS Human)

Use

Luciferase

Tags

luciferase

Expression

Mammalian

Mutation

3'UTR containing miRNA binding sites

Promoter

PGK

Available since

May 16, 2016

Availability

Academic Institutions and Nonprofits only

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PARP1 FL

Plasmid

#169815

Purpose

Expresses codon optimised full length His tagged PARP1 in bacterial cells

Depositor

David Neuhaus

Article

Ogden et al Nucleic Acids Res. 2021 Feb 26;49(4):226

Insert

Poly[ADP-ribose] polymerase 1 (PARP1 Synthetic, Human, Codon optimised)

Use

Tags

MKHHHHHHMKQ

Expression

Bacterial

Mutation

Valine 762 mutated to an alanine

Promoter

T7 promoter

Available since

Aug. 26, 2021

Availability

Academic Institutions and Nonprofits only

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pCS2-LRP6-3XFlag

Plasmid

#180144

Purpose

flag tagged LRP6

Depositor

Edward De Robertis

Article

Colozza et al Sci Rep. 2020 Dec 9;10(1):21555. doi: 10

Insert

Human LRP6 (LRP6 Human)

Use

Tags

FLAG

Expression

Mammalian

Mutation

Promoter

SP6

Available since

March 31, 2022

Availability

Academic Institutions and Nonprofits only

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tet_pLKO.1_puro_shNRF2 #1

Plasmid

#136584

Purpose

Expresses an inducible short hairpin targeting human NRF2 sequence

Depositor

Kevin Janes

Article

Pereira et al Sci Signal. 2020 Apr 14;13(627). pii: 13

Insert

shNRF2v1 (NFE2L2 Human)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

June 13, 2020

Availability

Academic Institutions and Nonprofits only

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pMP71-HLA-A0201-His

Plasmid

#108214

Purpose

HLA-A0201 his tagged

Depositor

Sébastien Walchli

Article

Lampen et al Immunohorizons. 2018 Jul 2;2(6):172-184.

Insert

major histocompatibility complex, class I, B (HLA-A Human)

Use

Retroviral

Tags

his

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 28, 2018

Availability

Academic Institutions and Nonprofits only

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pMXS-IRES-Blast POLA1

Plasmid

#160807

Purpose

Express POLA1

Depositor

Richard Possemato

Article

Sviderskiy et al Mol Cell. 2020 Oct 28. pii: S1097-2765(2

Insert

POLA1 (POLA1 Human)

Use

Retroviral

Tags

Expression

Mutation

Codon Optimized

Promoter

Available since

Jan. 20, 2021

Availability

Academic Institutions and Nonprofits only

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LentiGuide-hygro

Plasmid

#160090

Purpose

Expresses S. pyogenes CRISPR chimeric RNA element with customizable sgRNA from U6 promoter and hygromycin resistance marker

Depositor

Rizwan Haq

Article

Gstalder et al Cancer Discov. 2020 May 5. pii: 2159-829

Type

Empty backbone

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 13, 2020

Availability

Academic Institutions and Nonprofits only

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HA-Rab11-DN (S25N)

Plasmid

#101046

Purpose

Expresses HA-tagged human Rab11-DN (S25N) in mammalian cells

Depositor

Ken-Ichi Takemaru

Article

Burke et al J Cell Biol. 2014 Oct 13;207(1):123-37.

Insert

RAB11 (RAB11A Human)

Use

Tags

HA

Expression

Mammalian

Mutation

S25N

Promoter

CMV

Available since

Sept. 20, 2017

Availability

Academic Institutions and Nonprofits only

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SYKA-c020

Plasmid

#175489

Purpose

Protein expression in bacterial cells. Tandem SH2 domains, M6-N269. Can be used for crystallography.

Depositor

Opher Gileadi

Article

Bashore et al bioRxiv. 2023 Jul 29:2023.07.28.551026.

Insert

SYK (SYK Human)

Use

Tags

His6-TEV

Expression

Bacterial

Mutation

Promoter

Available since

Jan. 27, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA3 HA-PHLPP1 full length

Plasmid

#37100

Purpose

Expresses HA tagged, full-length human PHLPP1 in mammalian cells

Depositor

Alexandra Newton

Article

Warfel et al J Biol Chem. 2011 Jun 3;286(22):19777-88

Insert

PHLPP1 (PHLPP1 Human)

Use

Tags

HA

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Oct. 19, 2012

Availability

Academic Institutions and Nonprofits only

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pLV-EF1A-LivePAR(Y107A)-Hygro

Plasmid

#176073

Purpose

EGFP fused to the C-terminus of a WWE domain containing the mutation Tyr107Ala & a hygromycin resistance cassette

Depositor

Robert Sobol

Article

Koczor et al Cell Rep. 2021 Nov 2;37(5):109917. doi:

Insert

LivePAR (Y107A)

Use

Lentiviral

Tags

EGFP

Expression

Mammalian

Mutation

Point mutation to convert Try107 to Ala

Promoter

EF1A

Available since

Jan. 25, 2022

Availability

Academic Institutions and Nonprofits only

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pLenti-TRE3G-FNLS-PGK-Puro

Plasmid

#110847

Purpose

Lentiviral vector for dox-inducible expression of FNLS in mammalian cells (codon optimized)

Depositor

Lukas Dow

Article

Zafra et al Nat Biotechnol. 2018 Jul 3. pii: nbt.419

Insert

BE3RA-FNLS

Use

Lentiviral

Tags

3X FLAG

Expression

Mutation

D10A and NLS sequence at the N-terminus

Promoter

TRE3G

Available since

June 27, 2018

Availability

Academic Institutions and Nonprofits only

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Tol2-BetaActin-SecA5-YFP

Plasmid

#105664

Purpose

Ubiquitously expresses a secreted and fluorescently tagged Annexin5 for in-vivo apoptosis reporter

Depositor

Qing Deng

Article

Hsu et al Front Immunol. 2018 Mar 29;9:607. doi: 1

Insert

Secreted Annexin5-EYFP (ANXA5 Human)

Use

Tol2-gateway

Tags

EYFP

Expression

Mutation

Promoter

Beta-Actin

Available since

Feb. 14, 2018

Availability

Academic Institutions and Nonprofits only

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Lenti-DIO-P-EF1a-RPL22-3xHA-IRES-YFP

Plasmid

#170327

Purpose

Double-floxed inverse Orientation HA and IRES-YFP tagged RPL22

Depositor

Hemali Phatnani

Article

Gregory et al Cells. 2020 Jun 5;9(6). pii: cells906140

Insert

RPL22 (RPL22 Human)

Use

Lentiviral

Tags

Expression

Mutation

None

Promoter

Available since

May 17, 2021

Availability

Academic Institutions and Nonprofits only

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P-rTetO-EGFP-T2A-RPL22-3xHA

Plasmid

#170326

Purpose

Tetracycline-inducible expression of HA and EGFP tagged RPL22

Depositor

Hemali Phatnani

Article

Gregory et al Cells. 2020 Jun 5;9(6). pii: cells906140

Insert

RPL22 (RPL22 Human)

Use

Lentiviral

Tags

Expression

Mutation

None

Promoter

Available since

May 17, 2021

Availability

Academic Institutions and Nonprofits only

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tet_pLKO.1_puro_shNRF2 #2

Plasmid

#136585

Purpose

Expresses an inducible short hairpin targeting human NRF2 sequence

Depositor

Kevin Janes

Article

Pereira et al Sci Signal. 2020 Apr 14;13(627). pii: 13

Insert

shNRF2v2 (NFE2L2 Human)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

June 13, 2020

Availability

Academic Institutions and Nonprofits only

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P-Ef1a-RPL22-3XHA-P2A-EGFP-T2A-Puro

Plasmid

#170317

Purpose

Expresses HA and EGFP tagged RPL22, puromycin resistance

Depositor

Hemali Phatnani

Article

Gregory et al Cells. 2020 Jun 5;9(6). pii: cells906140

Insert

RPL22 (RPL22 Human)

Use

Lentiviral

Tags

Expression

Mutation

None

Promoter

Available since

May 17, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge