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plenti-UBC-gate-3xHA-pGK-PUR

Plasmid

#107393

Purpose

Lentiviral gateway destination vector

Depositor

William Kaelin

Article

Lu et al Science. 2014 Jan 17;343(6168):305-9. do

Type

Empty backbone

Use

Lentiviral; Gateway destination

Tags

3xHA

Expression

Mammalian

Available Since

May 22, 2018

Availability

Academic Institutions and Nonprofits only

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pLenti CMV/TO SV40 small + Large T (w612-1)

Plasmid

#22298

Depositor

Eric Campeau

Insert

SV40 small + Large T antigen

Use

Lentiviral

Expression

Mammalian

Available Since

Dec. 15, 2009

Availability

Academic Institutions and Nonprofits only

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plenti-ef1a-dCasRx-FTO-HA-T2A-BSD

Plasmid

#177120

Purpose

dCasRx mediated RNA tethering of FTO

Depositor

Wenbo Li

Article

Lee et al Mol Cell. 2021 Aug 19;81(16):3368-3385.e

Insert

dCasRx-FTO

Use

Lentiviral

Tags

HA and SV40 NLS

Mutation

dCasRX contains R239A/H244A/R858A/H863A mutations…

Promoter

Ef1a

Available Since

Nov. 24, 2021

Availability

Academic Institutions and Nonprofits only

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pLenti-EF1a-Luciferase-IRES-Blast-WPRE

Plasmid

#108542

Purpose

Lentivirus vector expressing Luciferase with blasticidin resistance

Depositor

Javier Alcudia

Inserts

Luciferase
Neomycin

Use

Lentiviral

Promoter

EF1apha and PGK

Available Since

April 19, 2018

Availability

Academic Institutions and Nonprofits only

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lenti sgRNA(MS2)_puro optimized backbone

Plasmid

#73797

Purpose

optimized lenti sgRNA cloning backbone with MS2 loops at tetraloop and stemloop 2 and EF1a-puro resistance marker. Contains BsmBI sites for insertion of spacer sequences.

Depositor

Feng Zhang

Article

Joung et al Nat Protoc. 2017 Apr;12(4):828-863. doi:

Has Service

Cloning Grade DNA

Type

Empty backbone

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

U6 and EF1A

Available Since

March 15, 2016

Availability

Academic Institutions and Nonprofits only

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plentiCMV-sct-pp65-b2m-HLA-A0201

Plasmid

#196511

Purpose

Express single-chain peptide-MHC (pp65 CMV epitope on HLA-A0201)

Depositor

Howard Chang

Article

Yu et al Cell. 2022 Dec 22;185(26):4904-4920.e22.

Insert

single chain of a pp65 peptide, b2m and HLA A0201

Use

Lentiviral

Tags

NA

Expression

Mammalian

Mutation

Y84C

Promoter

CMV

Available Since

March 1, 2023

Availability

Academic Institutions and Nonprofits only

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pLenti-EF1a-HIF2a dPA (P405A, P531A)

Plasmid

#232954

Purpose

Stably express a constitutively stable version of HIF2a (HIF2a dPA)

Depositor

William Kaelin

Article

Jiang et al Cell. 2025 Feb 25:S0092-8674(25)00156-4.

Insert

HIF2a (EPAS1 Human)

Use

Lentiviral

Expression

Mammalian

Mutation

P405A, P531A

Promoter

EF1a

Available Since

March 3, 2025

Availability

Academic Institutions and Nonprofits only

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Lenti-mCherry-G12V-KRAS-IRES-Blast

Plasmid

#153336

Purpose

Lentiviral vector plasmid for integration and constitutive expression of mCherry fused to oncogenic G12V-KRAS in mammalian cells

Depositor

Josef Penninger

Article

Chabloz et al Commun Biol. 2020 Jul 3;3(1):342. doi: 1

Insert

mCherry-G12V-KRAS (KRAS Human)

Use

Lentiviral

Expression

Mammalian

Available Since

July 21, 2020

Availability

Academic Institutions and Nonprofits only

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pLenti-hSFTPCpromoter(2kb)-EGFP-EF1a-TagRFP

Plasmid

#201681

Purpose

Lentiviral vector for human SFTPC promoter (2kb)-driven expression of EGFP and EF1a-driven TagRFP expression

Depositor

Emma Rawlins

Article

Lim et al bioRxiv [Preprint]. 2023 Sep 4:2023.08.3

Inserts

SFTPC promoter region (2kb upstream)
TagRFP

Use

Lentiviral

Expression

Mammalian

Available Since

Nov. 15, 2024

Availability

Academic Institutions and Nonprofits only

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pLenti-Synapsin-hChR2(H134R)-EYFP-WPRE

Plasmid

#20945

Purpose

3rd gen lentiviral expression of humanized ChR2 with H134R mutation fused to EYFP driven by human Synapsin I promoter for optogenetic activation

Depositor

Karl Deisseroth

Article

Zhang et al Nature. 2007 Apr 5. 446(7136):633-9.

Insert

channelrhodopsin-2

Use

Lentiviral

Expression

Mammalian

Mutation

H134R mutation in ChR2

Promoter

hSyn

Available Since

May 28, 2009

Availability

Academic Institutions and Nonprofits only

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pLenti-tetON-NOTUM-EF1a-TagRFP-2A-tet3G

Plasmid

#204188

Purpose

Doxycycline inducible lentiviral vector for human NOTUM overexpression

Depositor

Emma Rawlins

Article

Lim et al Cell Stem Cell. 2023 Jan 5;30(1):20-37.e

Insert

NOTUM (NOTUM Human)

Use

Lentiviral

Expression

Mammalian

Promoter

TetON

Available Since

Aug. 28, 2023

Availability

Academic Institutions and Nonprofits only

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pLenti-hU6-cs1-PuroR-T2A-BFP

Plasmid

#230934

Purpose

gRNA cloning vector with direct capture sequence 1 in stem loop 2

Depositor

Charles Gersbach

Article

Liu et al Cell Genom. 2025 Feb 4:100766. doi: 10.1

Type

Empty backbone

Use

CRISPR and Lentiviral

Tags

TagBFP

Available Since

Feb. 3, 2025

Availability

Academic Institutions and Nonprofits only

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Lenti-(BB)-EF1a-KRAB-dCas9-P2A-BlastR

Plasmid

#118154

Purpose

Catalytically inactive Cas9 from S. pyogenes with P2A-BlastR under the EF1a core promoter, and cloning backbone for sgRNA. Contains BsmBI sites for insertion of spacer sequences.

Depositor

Jorge Ferrer

Insert

KRAB-dCas9-P2A-BlastR

Use

CRISPR and Lentiviral

Tags

HA

Expression

Mammalian

Mutation

See depositor comments below

Promoter

EF1a core and U6

Available Since

March 27, 2019

Availability

Academic Institutions and Nonprofits only

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pLenti-tetON-NKX2-1-EF1a-TagRFP-2A-tet3G

Plasmid

#167942

Purpose

Inducible NKX2-1 overexpression

Depositor

Emma Rawlins

Article

Sun et al eLife. 2021 Oct 6;10. pii: 67886. doi: 1

Insert

TTF1 (NKX2-1 Human)

Use

Lentiviral

Promoter

tetON

Available Since

June 7, 2021

Availability

Academic Institutions and Nonprofits only

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plenti-CAG-gate-FLAG-IRES-GFP

Plasmid

#107398

Purpose

Lentiviral gateway destination vector

Depositor

William Kaelin

Article

Lu et al Science. 2014 Jan 17;343(6168):305-9. do

Type

Empty backbone

Use

Lentiviral; Gateway destination

Tags

FLAG

Expression

Mammalian

Available Since

May 17, 2018

Availability

Academic Institutions and Nonprofits only

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plenti-CAG-IKZF1-V1-FLAG-IRES-GFP

Plasmid

#107387

Purpose

Lentiviral expression of IKZF1-V1-FLAG

Depositor

William Kaelin

Article

Lu et al Science. 2014 Jan 17;343(6168):305-9. do

Insert

IKZF1-V1 (IKZF1 Human)

Use

Lentiviral

Tags

FLAG

Expression

Mammalian

Promoter

CAG

Available Since

June 7, 2018

Availability

Academic Institutions and Nonprofits only

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pLenti-PGK-Neo-PIP-NLS-mVenus

Plasmid

#118619

Purpose

Lentiviral expression of the cell cycle marker PIP-NLS-mVenus

Depositor

Jean Cook

Article

Grant et al Cell Cycle. 2018;17(21-22):2496-2516. do

Insert

PIP-NLS-mVenus

Use

Lentiviral

Tags

mVenus

Expression

Mammalian

Promoter

hPGK

Available Since

May 20, 2019

Availability

Academic Institutions and Nonprofits only

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Lenti-Trono-EFS-Blast-P2A-TurboRFP

Plasmid

#228893

Purpose

Cloning and expression of gRNAs or pegRNAs with EcoRI/Esp3I insertion.

Depositor

Francisco J Sánchez-Rivera

Article

Gould et al Nat Biotechnol. 2024 Mar 12. doi: 10.103

Type

Empty backbone

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

Dec. 6, 2024

Availability

Academic Institutions and Nonprofits only

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plenti-CAG-IRF4-FLAG-IRES-GFP

Plasmid

#107389

Purpose

Lentiviral expression of IRF4-FLAG

Depositor

William Kaelin

Article

Lu et al Science. 2014 Jan 17;343(6168):305-9. do

Insert

IRF4 (IRF4 Human)

Use

Lentiviral

Tags

FLAG

Expression

Mammalian

Promoter

CAG

Available Since

May 18, 2018

Availability

Academic Institutions and Nonprofits only

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pLenti-FNLS-P2A-GFP-PGK-Puro

Plasmid

#110869

Purpose

Lentiviral vector for constitutive expression of FNLS-P2A-GFP in mammalian cells (codon optimized)

Depositor

Lukas Dow

Article

Zafra et al Nat Biotechnol. 2018 Jul 3. pii: nbt.419

Insert

BE3RA-FNLS

Use

Lentiviral

Tags

3X FLAG

Mutation

D10A and NLS sequence at the N-terminus

Promoter

EF1s

Available Since

June 27, 2018

Availability

Academic Institutions and Nonprofits only

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