165,947 results

1. pTU1-A-pdh_RiboJ_mCherry_Bba_B0015

   Plasmid

   #107581

   Purpose

   B. megaterium DSM319 pdh promoter, mCherry (Bacillus codon optimised) for Bacillus cell-free transcription-translation. Bacillus shuttle vector backbone, colE1/AmpR (E. coli), RebB/TetA (Bacillus)

   Depositor

   Paul Freemont

   Article

   Moore et al Proc Natl Acad Sci U S A. 2018 Apr 17. p

   Insert

   mCherry
   

   Use

   Synthetic Biology

   Expression

   Bacterial

   Promoter

   pdh promoter Bacillus megaterium DSM319

   Available Since

   April 26, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. pYPQ131A2.0

   Plasmid

   #99884

   Purpose

   Golden Gate entry vector to express the 1st gRNA with gRNA2.0 scaffold (with four MS2 binding sites) under AtU6 promoter

   Depositor

   Yiping Qi

   Article

   Lowder et al Mol Plant. 2017 Nov 29. pii: S1674-2052(

   Type

   Empty backbone

   Use

   CRISPR

   Expression

   Plant

   Available Since

   March 8, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. pAAV.CAG.(mito).iATPSnFR2.A95A.A119L.miRFP670nano3

   Plasmid

   #209723

   Purpose

   Expresses mitochondrially targeted low affinity ratiometric ATP sensor

   Depositor

   Timothy Ryan

   Article

   Marvin et al Proc Natl Acad Sci U S A. 2024 May 21;12

   Insert

   (mito).iATPSnFR2.A95A.A119L.miRFP670nano3
   

   Use

   AAV

   Expression

   Mammalian

   Promoter

   CAG

   Available Since

   April 1, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pET-pA-Hia5

   Plasmid

   #174372

   Purpose

   SEE DEPOSITOR COMMENTS BELOW. Plasmid for IPTG-inducible expression and purification of protein A-Hia5-6xHis (pA-Hia5) for antibody directed DNA (adenine) methylation (as in DiMeLo-seq)

   Depositor

   Aaron Straight

   Article

   Altemose et al Nat Methods. 2022 Jun;19(6):711-723. doi

   Insert

   protein A - hia5
   

   Tags

   6xHis and Protein A

   Expression

   Bacterial

   Available Since

   Sept. 6, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pSLQ9824: CMV-SV40_NLS-CasMINI-V3.1--3xFlag- c-Myc_NLS-polyA

   Plasmid

   #176271

   Purpose

   Expression of CasMINI-V3.1 in mammalian cells.

   Depositor

   Stanley Qi

   Article

   Xu et al Mol Cell. 2021 Aug 26. pii: S1097-2765(2

   Insert

   CasMINI-V3.1
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Oct. 1, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pSM-mStayGold

   Plasmid

   #234317

   Purpose

   codon-optimized mStayGold with synthetic introns for the expression in C. elegans

   Depositor

   Kota Mizumoto

   Article

   Ko et al MicroPubl Biol. 2025 Jan 18;2025:10.1791

   Insert

   C. elegans codon optimized mStayGOld
   

   Tags

   mStayGold

   Expression

   Worm

   Available Since

   March 26, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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7. PB-TO-ASCL1-DLX2

   Plasmid

   #182307

   Purpose

   Piggybac Tet-ON plasmid for differentiating hiPSCs into GABAergic neuron via ASCL1 and DLX2 expression

   Depositor

   iPSC Neurodegenerative Disease Initiative (iNDI), Michael Ward

   Inserts

   Ascl1 (Ascl1 Mouse)
   Dlx2 (Dlx2 Mouse)
   puroR-T2A-mycNLS-mTagBFP2
   

   Tags

   None and T2A-mycNLS-mTagBFP2

   Expression

   Mammalian

   Promoter

   EF1a and pTRE3G-bidirectional

   Available Since

   April 14, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. pDEST53-LRRK2-WT

   Plasmid

   #25044

   Depositor

   Mark Cookson

   Article

   Greggio et al Neurobiol Dis. 2006 Aug . 23(2):329-41.

   Insert

   LRRK2 (LRRK2 Human)
   

   Tags

   GFP

   Expression

   Mammalian

   Available Since

   June 11, 2010

   Availability

   Academic Institutions and Nonprofits only

   

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9. pcDNA3/Myc-DNMT3B1

   Plasmid

   #35522

   Purpose

   Mammalian expression of human DNMT3B1 with myc tag

   Depositor

   Arthur Riggs

   Article

   Chen et al J Cell Biochem. 2005 Aug 1;95(5):902-17.

   Insert

   DNMT3B1 (DNMT3B Human)
   

   Tags

   Myc

   Expression

   Mammalian

   Available Since

   March 9, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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10. pET His6 MBP TEV LIC cloning vector (1M)

    Plasmid

    #29656

    Depositor

    Scott Gradia

    Has Service

    Cloning Grade DNA

    Type

    Empty backbone

    Tags

    6xHis, MBP, and TEV cleavage site

    Expression

    Bacterial

    Promoter

    T7-lacO (lactose/IPTG inducible)

    Available Since

    Aug. 17, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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11. pAAV.CAG.(mito).cpSFGFP.HaloTag

    Plasmid

    #214925

    Purpose

    Expresses mitochondrially targeted non-responsive control

    Depositor

    Timothy Ryan

    Article

    Marvin et al Proc Natl Acad Sci U S A. 2024 May 21;12

    Insert

    (mito).cpSFGFP.HaloTag
    

    Use

    AAV

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    April 10, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. pEGFP.90 beta

    Plasmid

    #108221

    Purpose

    Mammalian expression vector for EGFP fusion to human Hsp90 beta

    Depositor

    Didier Picard

    Article

    Picard et al Exp Cell Res. 2006 Nov 15. 312(19):3949-

    Insert

    EGFP-Hsp90b (HSP90AB1 Human)
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    April 5, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. lentiCRISPRv2 neo

    Plasmid

    #98292

    Purpose

    Variant of lentiCRISPRv2 that confers G418 resistance

    Depositor

    Brett Stringer

    Article

    Stringer et al Sci Rep. 2019 Mar 20;9(1):4902. doi: 10.

    Has Service

    Cloning Grade DNA

    Type

    Empty backbone

    Use

    CRISPR and Lentiviral

    Promoter

    EF-1a

    Available Since

    July 5, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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14. pK170.AAV-TRE-Cre-WPRE (Supernova)

    Plasmid

    #85040

    Purpose

    For AAV-TRE-Cre based Supernova, pK170 should be used with pK168 etc.

    Depositor

    Takuji Iwasato

    Article

    Luo et al Sci Rep. 2016 Oct 24;6:35747. doi: 10.10

    Insert

    nlsCre-WPRE
    

    Use

    AAV

    Expression

    Mammalian

    Available Since

    Nov. 30, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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15. pTUM4

    Plasmid

    #230905

    Purpose

    Plasmid encoding four periplasmic folding helper proteins to boost the secretion of functional heterologous proteins in E. coli

    Depositor

    Arne Skerra

    Article

    Schlapschy et al Protein Eng Des Sel. 2006 Aug;19(8):385-

    Inserts

    FkpA peptidyl-prolyl cis-trans isomerase FkpA (fkpA E. coli)
    SurA (surA E. coli)
    DsbA (dsbA E. coli)
    DsbC (dsbC E. coli)
    

    Expression

    Bacterial

    Available Since

    Feb. 5, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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16. AAVS1-DRGFP

    Plasmid

    #113193

    Purpose

    DR-GFP substrate (Addgene #26475) flanked by human AAVS1 homology arms. Endogenous AAVS1 promoter drives T2A-hygromycin expression after integration. DR-GFP cassette contains PGK-puromycin.

    Depositor

    Adam Karpf

    Insert

    DR-GFP
    

    Use

    Donor construct with t2a-hygromycin for gene trap…

    Expression

    Mammalian

    Available Since

    Oct. 31, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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17. 2Flag-pDEST-N

    Plasmid

    #118371

    Purpose

    Gateway compatible mammalian expression vector with N-terminal 2Flag to tag proteins of interest.

    Depositor

    Maria Vartiainen

    Article

    Viita et al J Cell Sci. 2019 Apr 17;132(8). pii: jcs

    Type

    Empty backbone

    Use

    Destination

    Tags

    2Flag

    Expression

    Mammalian

    Available Since

    Nov. 28, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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18. pcDNA3.1-3xFLAG-TEV-UBE2O

    Plasmid

    #105718

    Purpose

    Expresses WT full length human N- terminally tagged UBE2O

    Depositor

    Ramanujan Hegde

    Article

    Yanagitani et al Science. 2017 Aug 4;357(6350):472-475. d

    Insert

    UBE2O (UBE2O Human)
    

    Tags

    3xFLAG-TEV

    Expression

    Mammalian

    Promoter

    CMV (from vector)

    Available Since

    Feb. 6, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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19. pEGFP-2xFYVE

    Plasmid

    #140047

    Purpose

    Expresses GFP-tagged HRS 2xFYVE in mammalian cells

    Depositor

    Harald Stenmark

    Article

    Gillooly et al EMBO J. 2000 Sep 1;19(17):4577-88. doi:

    Insert

    tandem HRS FYVE domain (Hgs Mouse)
    

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    two FYVE domains (amino acids 147-223), linked by…

    Promoter

    CMV

    Available Since

    May 3, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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20. pBeloBac11loxP2272

    Plasmid

    #60342

    Purpose

    This is a BAC vector that contains both wt and mutant loxP sites.

    Depositor

    Jon Coren

    Article

    Coren et al BMC Res Notes. 2017 Jul 28;10(1):344. do

    Type

    Empty backbone

    Use

    Cre/Lox

    Expression

    Bacterial

    Available Since

    Aug. 12, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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