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pcDNA3.1-MS2-HA-HsRck_AC

Plasmid

#148417

Purpose

Mammalian Expression of HsRck

Depositor

Elisa Izaurralde

Article

Kuzuoglu-Ozturk et al EMBO J. 2016 Jun 1;35(11):1186-203. doi:

Insert

HsRck (DDX6 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pMT3 p38

Plasmid

#12658

Depositor

John Kyriakis

Insert

p38alpha (MAPK14 Human)

Use

Tags

HA

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 21, 2006

Availability

Academic Institutions and Nonprofits only

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pcDNA3-Tet1

Plasmid

#60938

Purpose

Expresses Tet1 in mammalian cells

Depositor

Yi Zhang

Article

Wang et al Cell Rep. 2014 Jan 30;6(2):278-84. doi:

Insert

Ten-Eleven Translocation 1 (Tet1 Mouse)

Use

Tags

flag

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Nov. 10, 2015

Availability

Academic Institutions and Nonprofits only

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pEVOL-pBpF

Plasmid

#31190

Purpose

tRNA synthetase/tRNA pair for the in vivo incorporation of a photocrosslinker, p-benzoyl-l-phenylalanine into proteins in E coli in response to the amber codon, TAG.

Depositor

Peter Schultz

Article

Chin et al Proc Natl Acad Sci U S A. 2002 Aug 20;99

Insert

M.j. p-benzoylphenylalanine RS (2 copies +tRNA)

Use

Tags

Expression

Bacterial

Mutation

Y32G, E107P, D158T, I159S

Promoter

Available since

Dec. 9, 2011

Availability

Academic Institutions and Nonprofits only

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phage UbiC tagRFP-T-DDX6

Plasmid

#119947

Purpose

Lentiviral vector expressing tagRFP-T-DDX6 fusion protein

Depositor

Jeffrey Chao

Article

Wilbertz et al Mol Cell. 2019 Jan 4. pii: S1097-2765(18

Insert

DDX6 (DDX6 Human)

Use

Lentiviral

Tags

tagRFP-T

Expression

Bacterial and Mammalian

Mutation

Promoter

UbiC

Available since

March 20, 2019

Availability

Academic Institutions and Nonprofits only

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pBBK28 pHomL-TDH3p-GFPdropout-Link-Neon(gfp)-SSA1t-HomR (AmpR)

Plasmid

#179012

Purpose

Parent vector for markerless yeast integration cassettes with C-terminal Neon(gfp)-tagged genes

Depositor

Vincent Martin

Article

Bean et al G3 (Bethesda). 2022 Jun 16. pii: 6609175

Type

Empty backbone

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

Aug. 5, 2022

Availability

Academic Institutions and Nonprofits only

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pBBK24 pCAS-Tyr-[gRNA: 7=HIS3] (SplitHygR, AmpR)

Plasmid

#179008

Purpose

Sp.Cas9 and gRNA yeast expression vector with HIS3 gRNA pre-cloned. Selection =SplitHygromycin resistance

Depositor

Vincent Martin

Article

Bean et al G3 (Bethesda). 2022 Jun 16. pii: 6609175

Insert

S. pyogenes Cas9

Use

CRISPR and Synthetic Biology

Tags

Expression

Yeast

Mutation

Promoter

Available since

May 3, 2022

Availability

Academic Institutions and Nonprofits only

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AbVec1.1-IGKC

Plasmid

#80796

Purpose

Expression of immunoglobulin light chains in mammalian cells, human kappa constant region

Depositor

Hedda Wardemann

Article

Tiller et al J Immunol Methods. 2008 Jan 1;329(1-2):1

Type

Empty backbone

Use

Tags

Expression

Mammalian

Mutation

Promoter

hCMV IE1 gene promoter

Available since

June 28, 2017

Availability

Academic Institutions and Nonprofits only

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spNW30

Plasmid

#173485

Purpose

Expresses spNW30 in Bl21

Depositor

Huan Bao

Article

Zhang et al Nat Commun. 2021 Sep 14;12(1):5451. doi:

Insert

spNW30

Use

Tags

Histag

Expression

Bacterial

Mutation

Promoter

Available since

Sept. 15, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA_RFP-TEVprotease

Plasmid

#124947

Purpose

Expression of RFP-TEVprotease

Depositor

Daniel Hammer, Matthew Good, Benjamin Schuster

Article

Schuster et al Nat Commun. 2018 Jul 30;9(1):2985. doi:

Insert

RFP-TEVprotease

Use

Tags

mCherry

Expression

Mammalian

Mutation

Promoter

Available since

July 30, 2019

Availability

Academic Institutions and Nonprofits only

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pCHW110035

Plasmid

#222444

Purpose

Level 0, N-terminal tag module (CCAT-AATG) containing LoxP-mCherry-stop.

Depositor

Chih-Hang Wu

Article

Chiang et al bioRxiv 2024.03.23.586378

Insert

LoxP-mCherry-stop

Use

Synthetic Biology

Tags

Expression

Plant

Mutation

no

Promoter

Available since

Nov. 21, 2024

Availability

Academic Institutions and Nonprofits only

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pEGFP-TYR

Plasmid

#32781

Depositor

Ruth Halaban

Article

Halaban et al Proc Natl Acad Sci U S A. 2000 May 23;97

Insert

Tyrosinase (TYR Human)

Use

Tags

EGFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Oct. 25, 2011

Availability

Academic Institutions and Nonprofits only

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pMBP-FnCas12a

Plasmid

#113432

Purpose

Expresses 10xHis-MBP fused to FnCas12a

Depositor

Jennifer Doudna

Article

Chen et al Science. 2018 Apr 27;360(6387):436-439.

Insert

FnCas12a

Use

Tags

MBP

Expression

Bacterial

Mutation

Promoter

T7

Available since

Aug. 1, 2018

Availability

Academic Institutions and Nonprofits only

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MSGV1-1D3-28Z.1-3 mut

Plasmid

#107227

Purpose

Anti-mouse CD19 CAR with CD3 ITAMs mutated

Depositor

Steven Rosenberg, James Kochenderfer

Article

Kochenderfer et al Blood. 2010 Nov 11;116(19):3875-86. doi:

Insert

1D3-28Z. 1-3 mut

Use

Retroviral

Tags

None

Expression

Mutation

None

Promoter

Available since

March 30, 2018

Availability

Academic Institutions and Nonprofits only

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pHAGE-TGFB1

Plasmid

#116799

Purpose

Lentiviral expression of TGFB1

Depositor

Kenneth Scott, Gordon Mills

Article

Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

Insert

TGFB1 (TGFB1 Human)

Use

Lentiviral

Tags

Expression

Mutation

WT

Promoter

Available since

March 14, 2019

Availability

Academic Institutions and Nonprofits only

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pCDNA3.1 hSCN5A

Plasmid

#145374

Purpose

expresses human Nav1.5 (WT) in mammalian cells

Depositor

Hugues Abriel, Robert Kass

Article

Rougier et al Am J Physiol Cell Physiol. 2005 Mar;288(

Insert

sodium voltage-gated channel alpha subunit 5 (SCN5A Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Oct. 28, 2020

Availability

Academic Institutions and Nonprofits only

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pFLP-hyg

Plasmid

#87831

Purpose

Expresses FLP recombinase with hyg selection marker

Depositor

Pep Charusanti

Article

Huang et al BMC Res Notes. 2014 Jan 8;7:13. doi: 10.

Insert

hyg

Use

Tags

None

Expression

Bacterial

Mutation

None

Promoter

Available since

Aug. 5, 2017

Availability

Academic Institutions and Nonprofits only

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pMSCV-hygro-STING

Plasmid

#102598

Purpose

Express STING for IFN-beta luciferase reporter assay.

Depositor

Nicolas Manel

Article

Cerboni et al J Exp Med. 2017 Jun 5;214(6):1769-1785.

Insert

STING (STING1 Human)

Use

Retroviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 22, 2018

Availability

Academic Institutions and Nonprofits only

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mEGFP-N1

Plasmid

#54767

Purpose

Localization: N1 Cloning Vector, Excitation: 488, Emission: 507

Depositor

Michael Davidson

Has Service

Cloning Grade DNA

Type

Empty backbone

Use

Tags

mEGFP

Expression

Mammalian

Mutation

Promoter

Available since

June 20, 2014

Availability

Industry, Academic Institutions, and Nonprofits

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pBBK18 pCAS-Tyr-[gRNA: 1=FF18] (SplitHygR, AmpR)

Plasmid

#179002

Purpose

Sp.Cas9 and gRNA yeast expression vector with FF18 gRNA pre-cloned. Selection =SplitHygromycin resistance

Depositor

Vincent Martin

Article

Bean et al G3 (Bethesda). 2022 Jun 16. pii: 6609175

Insert

S. pyogenes Cas9

Use

CRISPR and Synthetic Biology

Tags

Expression

Yeast

Mutation

Promoter

Available since

May 3, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge