8,736 results

1. pSNAPf-Cox8A Control Plasmid

   Plasmid

   #101129

   Purpose

   Expression of SNAP-Tagged Cox8A in Mammalian cells

   Depositor

   New England Biolabs, Ana Egana

   Insert

   Cox8A (COX8A Human)
   

   Use

   Tags

   SNAP-tag (SNAPf)

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Sept. 27, 2017

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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2. pNeae2

   Plasmid

   #168300

   Purpose

   Bacterial surface display of polypeptides (nanobodies) in E. coli

   Depositor

   Luis Ángel Fernández

   Article

   Salema et al PLoS One. 2013 Sep 23;8(9):e75126. doi:

   Insert

   Enterohemorraghic E. coli Intimin N-terminal fragment, E-tag, 6xHis tag, and myc-tag at the C-terminus
   

   Use

   Tags

   6xHis, E-tag, and myc-tag

   Expression

   Bacterial

   Mutation

   Promoter

   LacI-Plac

   Available since

   June 9, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pRho-DsRed

   Plasmid

   #11156

   Depositor

   Connie Cepko

   Article

   Matsuda et al Proc Natl Acad Sci U S A. 2004 Jan 6. 10

   Insert

   rhodopsin promoter (RHO Bovine)
   

   Use

   Tags

   DsRed2

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   May 25, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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4. cyto-Ruby3-iATPSnFR1.0

   Plasmid

   #102551

   Purpose

   expresses cytoplasmic ATP sensor in mammalian cells with red reference protein

   Depositor

   Baljit Khakh

   Article

   Lobas et al Nat Commun. 2019 Feb 12;10(1):711. doi:

   Insert

   Ruby3-iATPSnFR1.0
   

   Use

   Tags

   Ruby3

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Jan. 10, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. pcDNA3-EGFP-Cdc42(wt)

   Plasmid

   #12599

   Depositor

   Klaus Hahn

   Article

   Nalbant et al Science. 2004 Sep 10. 305(5690):1615-9.

   Insert

   EGFP-Cdc42 (WT) (CDC42 Human)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Oct. 16, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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6. pLIX-hN1ICD

   Plasmid

   #91897

   Purpose

   Doxycycline inducible expression of human Notch1 ICD

   Depositor

   Julien Sage

   Insert

   Notch1 intracellular domain (NOTCH1 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   codons 1770 to 2555 of human NOTCH1

   Promoter

   TRE promoter, Tet ON

   Available since

   June 6, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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7. DsRed2-Mito-7

   Plasmid

   #55838

   Purpose

   Localization: Mitochondria, Excitation: 558, Emission: 583

   Depositor

   Michael Davidson

   Insert

   Mito (COX8A Human)
   

   Use

   Tags

   DsRed2

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   March 12, 2015

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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8. pNBU2_erm-TetR-P1T_DP-GH023

   Plasmid

   #90324

   Purpose

   Integration vector at an attB site in Bacteroides; contains 1kb cassette with both the P1T_DP aTC-inducible promoter (RBS GH023) and the consitutively-driven TetR by the P2-A21 promoter

   Depositor

   Andrew Goodman

   Article

   Lim et al Cell. 2017 Apr 20;169(3):547-558.e15. do

   Type

   Empty backbone

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   May 31, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. pZS2-123

   Plasmid

   #26598

   Depositor

   Michael Elowitz

   Article

   Cox RS et al J Biol Eng. 2010 Jul 21;4:10. doi: 10.11

   Insert

   Synthetic
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   N/A

   Promoter

   Available since

   Feb. 23, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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10. pRK1043

    Plasmid

    #8835

    Depositor

    David Waugh

    Article

    Kapust et al Protein Eng. 2001 Dec . 14(12):993-1000.

    Insert

    tobacco etch virus protease, catalytic domain
    

    Use

    Tags

    MBP and polyarginine

    Expression

    Bacterial

    Mutation

    Autolysis-resistant S219V mutant

    Promoter

    Available since

    Feb. 14, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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11. pMT525

    Plasmid

    #131392

    Purpose

    L1RP retrotransposition reporter

    Depositor

    Kathleen Burns

    Article

    Ardeljan et al Nat Struct Mol Biol. 2020 Feb;27(2):168-

    Insert

    L1RP GFP-AI
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 8, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pBT372

    Plasmid

    #125613

    Purpose

    Expresses evoCDA1-BE4max-NG in mammalian cells

    Depositor

    David Liu

    Article

    Thuronyi et al Nat Biotechnol. 2019 Jul 22. pii: 10.103

    Insert

    evoCDA1-BE4max-NG
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    F23S A123V I195F

    Promoter

    Available since

    July 25, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. hLRP1-pEZT-BM

    Plasmid

    #177985

    Purpose

    Expression the full-length ectodomain of human LRP1 [residue 1-4414] in mammalian cells

    Depositor

    Lora Hooper

    Article

    Bang et al Science. 2021 Sep 17;373(6561):eabf9232.

    Insert

    LDL receptor related protein 1 (LRP1 Human)
    

    Use

    Tags

    No

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Dec. 15, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pDEST51-LRRK2-G2019S

    Plasmid

    #29401

    Depositor

    Mark Cookson

    Insert

    LRRK2 (LRRK2 Human)
    

    Use

    Destination vector

    Tags

    6XHis and V5

    Expression

    Mammalian

    Mutation

    Gly2019Ser

    Promoter

    Available since

    April 1, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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15. CD3 delta YFP

    Plasmid

    #11951

    Depositor

    Nico Dantuma

    Article

    Menendez-Benito et al Hum Mol Genet. 2005 Oct 1. 14(19):2787-9

    Insert

    CD3delta-YFP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 22, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCMV4-p52

    Plasmid

    #23289

    Depositor

    Warner Greene

    Article

    Beraud et al Mol Cell Biol. 1994 Feb . 14(2):1374-82.

    Insert

    p52 (NFKB2 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 31, 2010

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. PCV-Cas9

    Plasmid

    #123643

    Purpose

    Expresses Cas9 with an N-terminal HUH-tag called PCV2

    Depositor

    Wendy Gordon

    Article

    Aird et al Commun Biol. 2018 May 31;1:54. doi: 10.1

    Inserts

    PCV2 (ORF1 )
    Cas9
    

    Use

    Tags

    (EAAAK)8 linker and His6 + SUMO

    Expression

    Bacterial

    Mutation

    2-116

    Promoter

    Available since

    March 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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18. pcDNA3-ICUE3

    Plasmid

    #61622

    Purpose

    FRET-based biosensor for monitoring cAMP

    Depositor

    Jin Zhang

    Article

    DiPilato et al Mol Biosyst. 2009 Aug;5(8):832-7. doi: 1

    Insert

    ICUE3 (RAPGEF3 Human)
    

    Use

    Tags

    ECFP and cpVenus[L194]

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 13, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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19. H3K9me3 biosensor

    Plasmid

    #120802

    Purpose

    FRET biosensor. To monitor histone H3 lysine 9 tri-methylation in mammalian cells by FRET

    Depositor

    Yingxiao Wang

    Article

    Peng et al Proc Natl Acad Sci U S A. 2018 Nov 26. p

    Insert

    Truncated YPet-HP1-EV linker-ECFP-mouse histone H3
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 11, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. Rtn4a-GFP

    Plasmid

    #61807

    Purpose

    Human Rtn4a fluorescently tagged with GFP on the C-terminus for mammalian expression

    Depositor

    Gia Voeltz

    Article

    Shibata et al J Biol Chem. 2008 Jul 4. 283(27):18892-9

    Insert

    Rtn4a (RTN4 Human)
    

    Use

    Tags

    AcGFP

    Expression

    Mammalian

    Mutation

    Rtn4a Stop Codon removed

    Promoter

    CMV

    Available since

    Feb. 2, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge