We narrowed to 15,575 results for: NOL
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EK0499 SFFV-mCherry-SGc(PPIB.s1)cSG-EGFP-(3xMS2) (FLP-IN)
Plasmid#191183PurposeExpression of PPIB sensor in mammalian cellsDepositorInsertmCherry:PPIB.s1:EGFP-3xMS2
ExpressionMammalianMutationWTPromoterSFFVAvailable SinceOct. 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
pBM13-sd4U
Plasmid#179691PurposeBacterial expression of base editorDepositorInsert[sd4U] UGI-TALE3-DddA-G1397-N (Q1310R + S1330I + T1380I)
ExpressionBacterialMutationQ1310R, S1330I, T1380IAvailable SinceApril 4, 2022AvailabilityAcademic Institutions and Nonprofits only -
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EK0289 SFFV-mCherry-SG(s17d9b)SG-EGFP (FLP-IN)
Plasmid#191142PurposeExpression of Sensor 2 (for EK0211) in mammalian cells; mCherry marker, EGFP output.DepositorInsertmCherry:s17d9b:EGFP
ExpressionMammalianMutationWTPromoterSFFVAvailable SinceOct. 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
pJBL729
Plasmid#140399PurposeExpresses CadC-regulated 3WJdB RNA aptamer driven by T7 promoter with the cadO sequence 5BP downstream from the promoterDepositorInsertT7-cadO-3WJdB-T
UseSynthetic BiologyAvailable SinceJuly 8, 2020AvailabilityAcademic Institutions and Nonprofits only -
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pPV230.13
Plasmid#14902DepositorInsertStrongyloides stercoralis transitional endoplasmic reticulum ATPase promoter region
ExpressionWormMutationSs era-1 promoter region as a HindIII-PstI fragme…Available SinceJuly 18, 2007AvailabilityAcademic Institutions and Nonprofits only -
Zebrafish-gRNA-0007
Plasmid#42247PurposegRNA targeted to zebrafish gene th1DepositorAvailable SinceJan. 31, 2013AvailabilityAcademic Institutions and Nonprofits only -
pJBL718
Plasmid#140388PurposeExpresses MobR-regulated 3WJdB RNA aptamer driven by T7 promoter with the mobO sequence 5BP downstream from the promoterDepositorInsertT7-mobO-3WJdB-T
UseSynthetic BiologyAvailable SinceJuly 8, 2020AvailabilityAcademic Institutions and Nonprofits only -
pACYC-Skp-hLYZ
Plasmid#83924PurposeCo-expresses cytoplasmic copies of the Skp protein folding chaperone and wild type human lysozyme..DepositorAvailable SinceNov. 28, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCellFree_G03 PPARG
Plasmid#67099PurposeStandard for cell-free expression benchmarking.DepositorAvailable SinceNov. 6, 2015AvailabilityAcademic Institutions and Nonprofits only -
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pCoofy62
Plasmid#122014PurposeMammalian vector for parallel SLIC cloning containing the VEGF signal sequence for secretion, N-terminal His6-Smt3Star (SumoStar*) and different C-tag optionsDepositorTypeEmpty backboneTagsHis6-Smt3Star and Multi3: depending on the LP2 pr…ExpressionMammalianAvailable SinceMay 3, 2019AvailabilityAcademic Institutions and Nonprofits only -
pPB-DEST-R1R2-pNeo
Plasmid#141057PurposeMutisite Gateway mediated vector constructionDepositorInsertNeomycin resistance gene
ExpressionMammalianAvailable SinceJuly 7, 2020AvailabilityAcademic Institutions and Nonprofits only -
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pCfB6681
Plasmid#106157PurposeEasyCloneYALI system-based yeast integrative vector for markerfree integration via CRISPR/Cas9 to be used in combination with pCfB6637, integration into Yarrowia lipolytica chromosomal location IntE_3, USER site for cloning, amp resistanceDepositorTypeEmpty backboneExpressionYeastAvailable SinceMay 11, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCellFree_G03 PSMC1
Plasmid#67087PurposeStandard for cell-free expression benchmarking.DepositorInsertPSMC1 (PSMC1 Human)
UseCell-free expressionTagsEGFP and HisMutationE275G, I287V and R294GPromoterT7Available SinceNov. 6, 2015AvailabilityAcademic Institutions and Nonprofits only -
pTH1-SaSrtA-KA
Plasmid#64981PurposeExpresses the E105K/E108A mutant of Staphylococcus aureus sortase A in E. coliDepositorInsertsortase A
TagsHis6 tag, Mxe GyrA intein, and chitin-binding pro…ExpressionBacterialMutationdeleted amino acids 1-59, E105K, E108AAvailable SinceJune 5, 2015AvailabilityAcademic Institutions and Nonprofits only -
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pENTR-L1-PGK-Neo-L2
Plasmid#141007PurposeMutisite Gateway mediated vector constructionDepositorInsertNeomycin resistance gene
ExpressionMammalianAvailable SinceJuly 6, 2020AvailabilityAcademic Institutions and Nonprofits only -
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pMB1-spect-PthrC3-eGFP
Plasmid#107409PurposeThis plasmid contain promoter PthrC3, which is used for study about Short, Auto-inducible Promoters for Well-Controlled Protein Expression in Escherichia coli.DepositorInserteGFP
ExpressionBacterialPromoterPthrC3Available SinceMay 17, 2018AvailabilityAcademic Institutions and Nonprofits only -
pXylA-agrCA-IV
Plasmid#53438Purposexylose-dependent expression of the type-IV agrC and type-I agrA genes of S. aureusDepositorInsertagrCA locus
UseSynthetic BiologyTagsnoneExpressionBacterialMutation"MVQTS" sequence added to the N-terminu…PromoterPxylAAvailable SinceJune 3, 2014AvailabilityAcademic Institutions and Nonprofits only -
pEU3-NII-HLICNot
Plasmid#140181PurposeUsed for producing recombinant proteins by wheat germ-based cell-free translation, contains T7 promoter, 6x His-tag and NotI restriction siteDepositorTypeEmpty backboneUseExpression vector for a cell-free systemTagspEU3-NII-HLICNotPromoterT7Available SinceJune 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
pHCKan-yibDp-mdlC
Plasmid#134590PurposeLow Phosphate inducible mdlC benzylformate decarboxylaseDepositorInsertmdlC (benzylformate decarboxylase) C -terminal his tag
Tags6x-HisExpressionBacterialPromoteryibDpAvailable SinceMay 5, 2020AvailabilityAcademic Institutions and Nonprofits only -
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ROZA
Plasmid#64198Purpose"Reporter of Zap-70 Activity" Fluorescent reporter of ZAP-70 tyrosine kinase activityDepositorInsertROZA
ExpressionMammalianPromoterCMVAvailable SinceApril 23, 2015AvailabilityAcademic Institutions and Nonprofits only -
mKO-Golgi-7
Plasmid#57843PurposeLocalization: Golgi Complex, Excitation: 548, Emission: 561DepositorInsertGolgi (B4GALT1 Human)
TagsmKOExpressionMammalianMutationaa 1-82 of NM_001497.3PromoterCMVAvailable SinceOct. 24, 2014AvailabilityAcademic Institutions and Nonprofits only -
pEU3-NII-GLICNot
Plasmid#140182PurposeUsed for producing recombinant proteins by wheat germ-based cell-free translation, contains T7 promoter, GST-tag and NotI siteDepositorTypeEmpty backboneUseExpression vector for a cell-free systemTagsGSTPromoterT7Available SinceJune 23, 2020AvailabilityAcademic Institutions and Nonprofits only -
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pILGFPQ3
Plasmid#83558PurposeUsed to evaluate the expression output of GAL2 promoter with yEGFP used as the reporter gene.DepositorInsertGAL2 promoter-yEGFP
ExpressionYeastPromoterGAL2Available SinceDec. 15, 2016AvailabilityAcademic Institutions and Nonprofits only -
Gal4pBS4-Pleum
Plasmid#60329PurposeContains a promoter of a gal binding site coupled with a core promoter derived from native Leucine promoter.DepositorInsertpromoter
UseSynthetic Biology; Yeast synthetic hybrid promote…ExpressionYeastAvailable SinceFeb. 11, 2015AvailabilityIndustry, Academic Institutions, and Nonprofits -
pENTR-L3-Cas9-L2
Plasmid#141038PurposeMutisite Gateway mediated vector constructionDepositorInsertCas9
ExpressionMammalianAvailable SinceJune 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
VPCT-SUV39H2-L
Plasmid#89137Purposemammalian expression of full-length 410 amino acid SUV39H2 histone methyltransferase linked to VP16 activation domainDepositorAvailable SinceApril 10, 2017AvailabilityAcademic Institutions and Nonprofits only
