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pDEST-mScarlet-SH3PXD2A

Plasmid

#215534

Purpose

Expression vector with Tks5 tagged with N-terminal mScarlet

Depositor

Johanna Ivaska

Article

Conway et al Nat Cell Biol. 2025 May 26. doi: 10.1038

Insert

SH3PXD2A (SH3PXD2A Human)

Tags

mScarlet

Expression

Mammalian

Promoter

CMV

Available Since

May 29, 2025

Availability

Industry, Academic Institutions, and Nonprofits

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myc-Rictor

Plasmid

#1860

Purpose

Please see Addgene item #11367 for an updated version of this plasmid.

Depositor

David Sabatini

Article

Sarbassov et al Curr Biol 2004 Jul 27;14(14):1296-302.

Insert

Rictor (RICTOR Human)

Tags

Myc

Expression

Mammalian

Available Since

June 20, 2005

Availability

Academic Institutions and Nonprofits only

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pLEX304-mNeonGreen

Plasmid

#162034

Purpose

Tagged vector control

Depositor

David Bryant

Article

Nacke et al Nat Commun. 2021 Mar 12;12(1):1623. doi:

Type

Empty backbone

Use

Lentiviral

Tags

Neon Green

Available Since

Dec. 4, 2020

Availability

Academic Institutions and Nonprofits only

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pFX-EGFP-Rab7

Plasmid

#174457

Purpose

Express EGFP with late endosome-targeting sequence in mammalian cells

Depositor

Yusuke Ohba

Article

Kashiwagi et al Cell Struct Funct. 2019 Dec 26;44(2):183

Insert

EGFP with late endosome-targeting sequence

Tags

small GTPase (Rab7)

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 16, 2022

Availability

Academic Institutions and Nonprofits only

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HIVGKO

Plasmid

#112234

Purpose

HIVGKO contains a codon-switched eGFP under the control of the HIV-1 specific promoter, and mKO2 under the control of the constitutive promoter EF1a

Depositor

Eric Verdin

Article

Battivelli et al Elife. 2018 May 1;7. pii: 34655. doi: 10

Insert

csGFP-EF1a-mKO2

Use

Lentiviral

Available Since

Aug. 21, 2018

Availability

Academic Institutions and Nonprofits only

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Human V5-IRF3-pcDNA3

Plasmid

#32713

Depositor

Saumen Sarkar

Article

Zhu et al J Immunol. 2010 May 15;184(10):5768-76.

Insert

IRF3 (IRF3 Human)

Tags

V5

Expression

Mammalian

Promoter

CMV

Available Since

March 2, 2012

Availability

Academic Institutions and Nonprofits only

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pcDNA3 p53 WT

Plasmid

#69003

Purpose

mammalian expression of wild type human p53

Depositor

David Meek

Article

Loughery et al Nucleic Acids Res. 2014 Jul;42(12):7666-

Insert

p53 (TP53 Human)

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 9, 2015

Availability

Academic Institutions and Nonprofits only

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Lenti:FlipGFP-T2A-mCherry

Plasmid

#223198

Purpose

Lentiviral vector encoding FlipGFP-T2A-mCherry (w/ caspase-3 cleave site in FlipGFP for restoring GFP conformation)

Depositor

Yeh-Hsing Lao

Article

Chi et al Lab Chip. 2024 Jan 30;24(3):396-407. doi

Insert

FlipGFP-T2A-mCherry

Use

Lentiviral

Expression

Mammalian

Promoter

EF1a

Available Since

Sept. 12, 2024

Availability

Academic Institutions and Nonprofits only

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PLG-bio-His

Plasmid

#53427

Purpose

Expresses full-length Plasminogen precursor ectodomain in mammalian cells. C-terminal rat Cd4d3+4 tag, biotinylation sequence and His tag.

Depositor

Gavin Wright

Article

Sun et al Mol Cell Proteomics. 2015 Feb 23. pii: m

Insert

PLG (PLG Human)

Tags

His tag, enzymatic biotinylation sequence, and ra…

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 27, 2015

Availability

Academic Institutions and Nonprofits only

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pFX-mCherry-Rab7

Plasmid

#174458

Purpose

Express mCherry with late endosome-targeting sequence in mammalian cells

Depositor

Yusuke Ohba

Article

Kashiwagi et al Cell Struct Funct. 2019 Dec 26;44(2):183

Insert

mCherry with late endosome-targeting sequence

Tags

small GTPase (Rab7)

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 16, 2022

Availability

Academic Institutions and Nonprofits only

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pOsTIR1w/oGFP

Plasmid

#102883

Purpose

Expresses OsTIR1 via the ADH1 promoter in yeast. The cassette is integrated in the HO locus. Assembly and characterization of the auxin-inducible degradation.

Depositor

Matthias Heinemann

Article

Papagiannakis et al Mol Cell. 2017 Jan 19;65(2):285-295. doi

Insert

OsTIR1

Tags

3x myc

Expression

Yeast

Promoter

ADH1

Available Since

Jan. 5, 2018

Availability

Academic Institutions and Nonprofits only

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HB901: pMVP (L3-L2) polyA

Plasmid

#121746

Purpose

pMVP L3-L2 entry plasmid, contains human growth hormone polyadenylation signal (polyA) for 3- or 4-component MultiSite Gateway Pro assembly

Depositor

Christopher Newgard

Article

Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

Insert

Polyadenylation signal (polyA)

Use

Synthetic Biology; Pmvp gateway entry plasmid

Available Since

June 27, 2019

Availability

Academic Institutions and Nonprofits only

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pLenti-myc-GLUT4-mCherry

Plasmid

#64049

Purpose

3rd generation lentiviral transfer plasmid. Reporter for GLUT4 translocation

Depositor

Weiping Han

Article

Lim et al Nat Commun. 2015 Jan 9;6:5951. doi: 10.1

Insert

GLUT4 (Slc2a4 Mouse)

Use

Lentiviral

Tags

c-Myc and mCherry

Expression

Mammalian

Promoter

Ubiquitin

Available Since

July 10, 2015

Availability

Academic Institutions and Nonprofits only

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pcDNA3.2/Envelope

Plasmid

#120821

Purpose

Ectopic expression of ZIKV protein

Depositor

Jan Rehwinkel

Article

Hertzog et al Eur J Immunol. 2018 Mar 23. doi: 10.1002

Insert

Envelope ZIKV

Tags

V5

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 5, 2019

Availability

Academic Institutions and Nonprofits only

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PB533-44B12m23-sfGFP

Plasmid

#186778

Purpose

RNAP2 Ser5ph-mintbody expression vector for mammalian cells

Depositor

Hiroshi Kimura

Article

Ohishi et al Nat Commun. 2022 Dec 20;13(1):7672. doi:

Insert

44B12m23-scFv

Tags

sfGFP

Expression

Mammalian

Available Since

Aug. 9, 2022

Availability

Academic Institutions and Nonprofits only

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pCMV-mCherry-MHC-IIA

Plasmid

#35687

Depositor

Venkaiah Betapudi

Article

Dulyaninova et al Mol Biol Cell. 2007 Aug;18(8):3144-55. E

Insert

MHC IIA (MYH9 Human)

Tags

mCherry

Expression

Mammalian

Promoter

CMV

Available Since

April 24, 2012

Availability

Academic Institutions and Nonprofits only

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pQE60 R196A wgMDH

Plasmid

#204271

Purpose

Bacterial expression of R196A wgMDH with IPTG induction - Active site mutant

Depositor

Malate dehydrogenase CUREs Community (MCC)

Article

Cox et al FEBS J. 2005 Feb;272(3):643-54. doi: 10.

Insert

MDHG_CITLA

Tags

6X His

Expression

Bacterial

Mutation

R196A

Available Since

Oct. 23, 2023

Availability

Academic Institutions and Nonprofits only

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phage ubc nls ha 2xmcp HALO

Plasmid

#64540

Purpose

Used to label reporter mRNAs. Lentiviral expression of the MCP protein fused to HALO.

Depositor

Jeffrey Chao

Article

Halstead et al Science. 2015 Mar 20;347(6228):1367-671.

Insert

MCP

Use

Lentiviral

Tags

HA, HALO, and NLS

Expression

Mammalian

Promoter

human ubiquitin C promoter

Available Since

May 1, 2015

Availability

Academic Institutions and Nonprofits only

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MPRA Empty Vector

Plasmid

#204865

Purpose

Vector used for high-throughput cloning and subsequent transfection of MPRA libraries into mammalian cells.

Depositor

Philipp Maass

Article

Oliveros et al Cell Genom. 2023 May 24;3(7):100330. doi

Type

Empty backbone

Expression

Bacterial and Mammalian

Available Since

Sept. 5, 2023

Availability

Academic Institutions and Nonprofits only

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pEGFP-C1-hArfaptin2

Plasmid

#206405

Purpose

Mammalian expression of hArfaptin2

Depositor

Kazuhisa Nakayama, Hye-Won Shin

Article

Shin et al J Cell Sci. 2005 Sep 1;118(Pt 17):4039-4

Insert

Arfaptin2 (ARFIP2 Human)

Tags

EGFP

Expression

Mammalian

Available Since

Oct. 19, 2023

Availability

Academic Institutions and Nonprofits only

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