We narrowed to 911 results for: tes/1000

1. pcDNA3.1+_FH-AGO2-WT

   Plasmid

   #92006

   Purpose

   Expresses FLAG-HA-AGO2 (WT)

   Depositor

   Joshua Mendell

   Article

   Golden et al Nature. 2017 Feb 9;542(7640):197-202. do

   Insert

   AGO2 (AGO2 Human)
   

   Tags

   FLAG-HA

   Expression

   Mammalian

   Available Since

   Aug. 25, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. pcDNA3.3−3xFLAG−AGO2(D669A)

   Plasmid

   #220461

   Purpose

   Express catalytically-dead mutant human AGO2 with N-terminal 3xFLAG tag in mammalian cells

   Depositor

   David Bartel

   Article

   Wang et al Mol Cell. 2024 Jul 10:S1097-2765(24)0053

   Insert

   Human AGO2 (AGO2 Human)
   

   Tags

   3xFLAG

   Expression

   Mammalian

   Mutation

   Aspartate 669 to alanine

   Promoter

   CMV

   Available Since

   July 17, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pRSF-MjCouRS

   Plasmid

   #229066

   Purpose

   tRNA synthetase/tRNA pair for the in vivo incorporation of (7-hydroxy-4-coumarin-yl) ethylglycine (Hco), into proteins in E. coli in response to the amber (TAG) codon

   Depositor

   Thomas Huber

   Article

   Qianzhu et al Angew Chem Int Ed Engl. 2024 Dec 4:e2024

   Insert

   Methanococcus jannaschii tRNA synthetase
   

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Jan. 30, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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4. Arrb1-RFP

   Plasmid

   #35401

   Depositor

   Robert Lefkowitz

   Article

   Hara et al Nature. 2011 Aug 21;477(7364):349-53. do

   Insert

   beta-arrestin-1 (Arrb1 Rat)
   

   Tags

   RFP

   Expression

   Mammalian

   Promoter

   T7

   Available Since

   March 2, 2012

   Availability

   Academic Institutions and Nonprofits only

5. pEGFP-hGABARAP

   Plasmid

   #87871

   Purpose

   Express a EGFP-human GABARAP in mammalian cells

   Depositor

   Eiki Kominami, Isei Tanida

   Article

   Tanida et al J Biol Chem. 2001 Jan 19;276(3):1701-6.

   Insert

   GABARAP (GABARAP Human)
   

   Expression

   Mammalian

   Available Since

   Aug. 5, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. pETM33_EIF4E_eiF4E

   Plasmid

   #178474

   Purpose

   Bacterial expression of human domain with His-tag and GST tag

   Depositor

   Ylva Ivarsson

   Article

   Benz et al Mol Syst Biol. 2022 Jan;18(1):e10584. do

   Insert

   EIF4E_eiF4E (EIF4E Human, Synthetic)
   

   Tags

   His-GST

   Expression

   Bacterial

   Promoter

   T7/LacO

   Available Since

   March 22, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. MAP4K3-GFP

   Plasmid

   #21731

   Depositor

   L. Miguel Martins

   Article

   Lam et al Proc Natl Acad Sci U S A. 2009 Jul 21;10

   Insert

   MAP4K3 (MAP4K3 Human)
   

   Tags

   GFP

   Expression

   Mammalian

   Available Since

   Aug. 11, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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8. pPBpuro-EGFR(M721)

   Plasmid

   #197379

   Purpose

   Encoding human ErbB1 mutant.

   Depositor

   Michiyuki Matsuda

   Article

   Matsuda et al J Cell Sci. 2023 Jul 31:jcs.261199. doi:

   Insert

   EGFR mutant (EGFR Human)
   

   Expression

   Mammalian

   Mutation

   Lysine 721 to Methionine

   Available Since

   Aug. 7, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. pSpCas9_U6_sgRNA_Mettl3C

   Plasmid

   #165420

   Purpose

   sgRNA construct for targeting Mettl3 C-terminus

   Depositor

   Neil Brockdorff

   Article

   Wei et al Genome Res. 2021 Aug;31(8):1395-1408. do

   Insert

   PspCas9 gRNA targeting Mettl3 C-terminus (Mettl3 Mouse)
   

   Use

   CRISPR

   Expression

   Mammalian

   Available Since

   Oct. 15, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. pMIR-Dsred-IRES-His-Halo-Tev-Keap C151S/C288S

    Plasmid

    #62460

    Purpose

    expresses C151S and C288S double mutant human Keap1 protein

    Depositor

    Yimon Aye

    Article

    Parvez et al J Am Chem Soc. 2014 Dec 31.

    Insert

    Keap1 (KEAP1 Human)
    

    Tags

    His-Halo-Tev

    Expression

    Mammalian

    Mutation

    Changed Cysteines 151 and 288 of Keap1 to respect…

    Promoter

    CMV

    Available Since

    March 21, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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11. px330-CD4

    Plasmid

    #136938

    Purpose

    NHEJ assay. sgRNA/Cas9 plasmid. Target DSB at human CD4; induce CD4+ deletion rearrangement by pairing w/ px330-GAPDH

    Depositor

    Jeremy Stark

    Article

    Bhargava et al J Biol Chem. 2019 Nov 21. pii: RA119.010

    Insert

    sgRNA targeting CD4 (CD4 Human)
    

    Use

    CRISPR

    Expression

    Mammalian

    Available Since

    Feb. 14, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. pLNCX2-STIM1

    Plasmid

    #89817

    Purpose

    Express HUMAN STIM1

    Depositor

    Shengyu Yang

    Article

    Sun et al J Cell Biol. 2014 Nov 24;207(4):535-48.

    Insert

    STIM1 (STIM1 Human)
    

    Use

    Retroviral

    Tags

    NO TAG

    Available Since

    June 13, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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13. EGFP-Rab7a-7

    Plasmid

    #56443

    Purpose

    Localization: Ras GTPase, Excitation: 488, Emission: 507

    Depositor

    Michael Davidson

    Article

    Fang et al Traffic. 2010 Jun;11(6):782-99. doi: 10.

    Insert

    Rab7a (RAB7A Human)
    

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    N117S in NM_004637.5

    Promoter

    CMV

    Available Since

    Jan. 13, 2015

    Availability

    Industry, Academic Institutions, and Nonprofits

14. pSUPER-retro-puro-shSTIM1

    Plasmid

    #89816

    Purpose

    Knockdown human STIM1 expression

    Depositor

    Shengyu Yang

    Article

    Sun et al J Cell Biol. 2014 Nov 24;207(4):535-48.

    Insert

    STIM1 shRNA (STIM1 Human)
    

    Use

    Retroviral

    Available Since

    May 19, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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15. Arrb1-Q394L

    Plasmid

    #35402

    Depositor

    Robert Lefkowitz

    Article

    Hara et al Nature. 2011 Aug 21;477(7364):349-53. do

    Insert

    beta-arrestin-1 (Arrb1 Rat)
    

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Q394L

    Promoter

    CMV

    Available Since

    March 2, 2012

    Availability

    Academic Institutions and Nonprofits only

16. MIGR1-CD5

    Plasmid

    #159329

    Purpose

    Over-expression of mouse CD5

    Depositor

    Nevil Singh

    Article

    Matson et al Proc Natl Acad Sci U S A. 2020 Jun 23;11

    Insert

    CD5 (Cd5 Mouse)
    

    Use

    Retroviral

    Promoter

    LTR

    Available Since

    Dec. 15, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCS2.0 Tag-RFP-T-UtrCH

    Plasmid

    #101279

    Purpose

    Life imaging pCS2.0 Tag-RFP-T_UtrCH

    Depositor

    Alpha Yap

    Article

    Wu et al Nat Cell Biol. 2014 Feb;16(2):167-78. do

    Insert

    Utrophin (UTRN Human)
    

    Tags

    TagRFP-T

    Expression

    Mammalian

    Available Since

    Oct. 23, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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18. pcDNA3.3−3xFLAG−AGO2(S387D)

    Plasmid

    #220463

    Purpose

    Express S387 phosphomimetic mutant human AGO2 with N-terminal 3xFLAG tag in mammalian cells

    Depositor

    David Bartel

    Article

    Wang et al Mol Cell. 2024 Jul 10:S1097-2765(24)0053

    Insert

    Human AGO2 (AGO2 Human)
    

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    Serine 387 to aspartate

    Promoter

    CMV

    Available Since

    July 17, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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19. ePB-PURO-FLAG-METTL3

    Plasmid

    #160252

    Purpose

    For stable integration of FLAG-METTL3 in human cells with trasposase

    Depositor

    Alessandro Fatica

    Article

    Sorci et al Cell Death Dis. 2018 Jul 23;9(8):796. do

    Insert

    methyltransferase like 3 (METTL3 Human)
    

    Tags

    FLAG

    Expression

    Mammalian

    Promoter

    tight TRE promoter

    Available Since

    Nov. 18, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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20. pT5T_hp53_ST

    Plasmid

    #34922

    Depositor

    Thanos Halazonetis

    Article

    Petty et al EMBO J. 2011 Jun 1;30(11):2167-76. Epub

    Insert

    p53 DNA-binding (Res 1-393) (TP53 Human)
    

    Expression

    Bacterial

    Mutation

    C135V, C141V, W146Y, C182S, V203A, R209P, C229Y, …

    Promoter

    T7

    Available Since

    Feb. 22, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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21. pGEX 4T1 RARalpha

    Plasmid

    #35557

    Depositor

    Harish Srinivas, Jonathan Kurie

    Article

    Srinivas et al Mol Cell Biol. 2005 Feb;25(3):1054-69.

    Insert

    RARalpha (RARA Human)
    

    Tags

    GST

    Expression

    Bacterial

    Available Since

    April 5, 2012

    Availability

    Academic Institutions and Nonprofits only

22. B1 Integrin-GFP

    Plasmid

    #186138

    Purpose

    For expression of recombinant beta1 integrin cytosolic domain with C-terminal GFP tag in bacteria

    Depositor

    Michael Rosen

    Article

    Case et al Elife. 2022 Jan 20;11. pii: 72588. doi:

    Insert

    ITGB1 (ITGB1 Human)
    

    Tags

    MBP and mEGFP

    Expression

    Bacterial

    Promoter

    Lac

    Available Since

    July 11, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pGL4.24-Cd36-enhancer4

    Plasmid

    #138576

    Purpose

    Firefly luciferase enhancer reporter fused with 1.5~2 kb fragments of mouse Cd36 enhancers at Chr5:17876439_17877752

    Depositor

    Lora Hooper

    Article

    Kuang et al Science. 2019 Sep 27;365(6460):1428-1434

    Insert

    Cd36 Promoter (Cd36 Mouse)
    

    Use

    Luciferase

    Promoter

    Minimal promoter

    Available Since

    March 26, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pGL4.24-Cd36-enhancer1

    Plasmid

    #138573

    Purpose

    Firefly luciferase enhancer reporter fused with 1.5~2 kb fragments of mouse Cd36 enhancers at Chr5:17835541_17837355

    Depositor

    Lora Hooper

    Article

    Kuang et al Science. 2019 Sep 27;365(6460):1428-1434

    Insert

    Cd36 enhancer (Cd36 Mouse)
    

    Use

    Luciferase

    Promoter

    Minimal promoter

    Available Since

    March 26, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pcDNA3.1+_LN-HA-AGO2-WT

    Plasmid

    #92009

    Purpose

    Expresses Lambda-N-HA-AGO2 (WT)

    Depositor

    Joshua Mendell

    Article

    Golden et al Nature. 2017 Feb 9;542(7640):197-202. do

    Insert

    AGO2 (AGO2 Human)
    

    Tags

    Lambda-N-HA

    Expression

    Mammalian

    Available Since

    Aug. 25, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pPBpuro-ErbB1(DEL1011)

    Plasmid

    #197366

    Purpose

    Encoding human ErbB1 mutant.

    Depositor

    Michiyuki Matsuda

    Article

    Matsuda et al J Cell Sci. 2023 Jul 31:jcs.261199. doi:

    Insert

    EGFR mutant. (EGFR Human)
    

    Expression

    Mammalian

    Mutation

    deleted amino acids 1011-1186

    Available Since

    Aug. 7, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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27. pcDNA-Cx43

    Plasmid

    #60691

    Purpose

    mammalian expression of rat Cx43

    Depositor

    Parmender Mehta

    Article

    Johnson et al Mol Biol Cell. 2013 Mar;24(6):715-33. do

    Insert

    Cx43 (Gja1 Rat)
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    July 6, 2015

    Availability

    Academic Institutions and Nonprofits only

28. pIRESneo-FLAG/HA Ago2

    Plasmid

    #10821

    Depositor

    Thomas Tuschl

    Article

    Meister et al Mol Cell. 2004 Jul 23. 15(2):185-97.

    Insert

    Argonaute 2 (AGO2 Human)
    

    Tags

    FLAG and HA

    Expression

    Mammalian

    Mutation

    R668H. Additionally, N terminus different from wi…

    Available Since

    Oct. 14, 2005

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. tet-pLKO shMKP5.v2 puro

    Plasmid

    #87793

    Purpose

    Expresses an inducible short hairpin targeting human MKP5 sequence

    Depositor

    Kevin Janes

    Article

    Shah et al Mol Cell Proteomics. 2017 Apr;16(4 suppl

    Insert

    shMKP5 (DUSP10 Human)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    TRE tight

    Available Since

    March 31, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. tet-pLKO shMKP5.v1 puro

    Plasmid

    #87792

    Purpose

    Expresses an inducible short hairpin targeting human MKP5 sequence

    Depositor

    Kevin Janes

    Article

    Shah et al Mol Cell Proteomics. 2017 Apr;16(4 suppl

    Insert

    shMKP5 (DUSP10 Human)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    TRE tight

    Available Since

    March 27, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge