We narrowed to 2,608 results for: ERK

Showing: 481 - 520 of 2608 results

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pGEX6P1-HA-MEK5

Plasmid

#53166

Purpose

Expresses GST-HA tagged human MEK5 from bacterial expression vector

Depositor

Christopher Counter

Article

Brady et al Nature. 2014 Apr 9. doi: 10.1038/nature1

Insert

MEK5 (MAP2K5 Human)

Tags

GST and HA

Expression

Bacterial

Available Since

June 24, 2014

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-HaloEKAR2.2-T2A-EGFP

Plasmid

#216495

Purpose

Expression of chemigenetic ERK biosensor (low basal brightness, extremly high dynamic range) and EGFP transfection marker in mammalian cells

Depositor

Jin Zhang

Article

Frei et al Nat Biotechnol. 2025 Apr 21. doi: 10.103

Insert

HaloEKAR2.2

Tags

T2A-EGFP

Expression

Mammalian

Promoter

CMV

Available Since

June 20, 2025

Availability

Academic Institutions and Nonprofits only

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pGEX6P1-HA-MEK1

Plasmid

#53159

Purpose

Expresses GST-HA tagged human MEK1 from bacterial expression vector

Depositor

Christopher Counter

Article

Brady et al Nature. 2014 Apr 9. doi: 10.1038/nature1

Insert

MEK1 (MAP2K1 Human)

Tags

GST and HA

Expression

Bacterial

Available Since

June 12, 2014

Availability

Academic Institutions and Nonprofits only

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pKrox24(2xD-E_inD)Luc

Plasmid

#200109

Purpose

Luciferase reporter for in cell monitoring of receptor tyrosine kinase-MAP ERK pathway activity

Depositor

Pavel Krejčí

Article

Gudernova et al Elife. 2017 Feb 15;6. pii: e21536. doi:

Insert

modified EGR1 promoter

Expression

Mammalian

Mutation

highly modified sequence

Promoter

2 copies of D-E element in front of EGR1 promoter…

Available Since

Nov. 20, 2023

Availability

Academic Institutions and Nonprofits only

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pCEP4-myc-EphB2

Plasmid

#200982

Purpose

Mammalian expression plasmid for myc-tagged EphB2

Depositor

Erik Procko

Article

Narayanan et al J Virol. 2023 Nov 30;97(11):e0062123. do

Insert

EphB2 (EPHB2 Human)

Tags

Extracellular c-myc epitope tag and Signal peptid…

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 2, 2023

Availability

Academic Institutions and Nonprofits only

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MEF2A-Sensor

Plasmid

#208928

Purpose

in vitro MAPK-Sensor for luminescent detection of ERK and p38-activity

Depositor

Attila Reményi

Article

Poti et al Int J Mol Sci. 2023 Oct 3;24(19):14854.

Insert

Mef2a docking sequence+Phospho site (MEF2A Human)

Tags

HISx6, MBP, and Smbit

Expression

Bacterial

Promoter

T7

Available Since

April 2, 2024

Availability

Academic Institutions and Nonprofits only

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pWZLblasti-HA-MEK1

Plasmid

#53161

Purpose

Expresses HA tagged human MEK1 from blasticidin resistance retroviral vector

Depositor

Christopher Counter

Article

Brady et al Nature. 2014 Apr 9. doi: 10.1038/nature1

Insert

MEK1 (MAP2K1 Human)

Use

Retroviral

Tags

HA

Expression

Mammalian

Available Since

May 28, 2014

Availability

Academic Institutions and Nonprofits only
pcDNA3-Venus-cpVenus-FLARE-EKAR-EV

Plasmid

#123339

Purpose

Yellow-fluorescent homoFRET/anisotropy-based biosensor for monitoring ERK activity. Contains monomeric Venus/cpVenus-E172 homoFRET pair.

Depositor

Jin Zhang

Article

Ross et al eLife. 2018 Jul 3;7. pii: 35458. doi: 10

Insert

Venus-cpVenus-FLARE-EKAR-EV

Tags

6xHIS, T7 tag (gene 10 leader), Venus, Xpress (TM…

Expression

Mammalian

Promoter

CMV

Available Since

July 28, 2020

Availability

Academic Institutions and Nonprofits only

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pWZLblasti-HA-MEK1 C121S

Plasmid

#53165

Purpose

Expresses HA tagged human MEK1 C121S from blasticidin resistance retroviral vector

Depositor

Christopher Counter

Article

Brady et al Nature. 2014 Apr 9. doi: 10.1038/nature1

Insert

MEK1 (MAP2K1 Human)

Use

Retroviral

Tags

HA

Expression

Mammalian

Mutation

C121S

Available Since

June 24, 2014

Availability

Academic Institutions and Nonprofits only
pHH0103_MAPK3_Pkinase

Plasmid

#110023

Purpose

Protein expression and purification of MAPK3_Pkinase

Depositor

Sachdev Sidhu

Article

Teyra et al Mol Syst Biol. 2020 Dec;16(12):e9310. do

Insert

MAPK3_Pkinase (MAPK3 Human)

Expression

Bacterial

Available Since

Oct. 28, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA3-Cerulean3-Cerulean3-FLARE-EKAR-EV

Plasmid

#123342

Purpose

Cyan-fluorescent homoFRET/anisotropy-based biosensor for monitoring ERK activity.

Depositor

Jin Zhang

Article

Ross et al eLife. 2018 Jul 3;7. pii: 35458. doi: 10

Insert

Cerulean3-Cerulean3-FLARE-EKAR-EV

Tags

6xHIS, Cerulean3, T7 tag (gene 10 leader), and Xp…

Expression

Mammalian

Promoter

CMV

Available Since

July 28, 2020

Availability

Academic Institutions and Nonprofits only

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pGEX6P1-HA-MEK1 C121S

Plasmid

#53164

Purpose

Expresses GST-HA tagged human MEK1 C121S from bacterial expression vector

Depositor

Christopher Counter

Article

Brady et al Nature. 2014 Apr 9. doi: 10.1038/nature1

Insert

MEK1 (MAP2K1 Human)

Tags

GST and HA

Expression

Bacterial

Mutation

C121S

Available Since

June 9, 2014

Availability

Academic Institutions and Nonprofits only
pWZLblasti-HA-MEK5

Plasmid

#53167

Purpose

Expresses HA tagged human MEK5 from blasticidin resistance retroviral vector

Depositor

Christopher Counter

Article

Brady et al Nature. 2014 Apr 9. doi: 10.1038/nature1

Insert

MEK5 (MAP2K5 Human)

Use

Retroviral

Tags

HA

Expression

Mammalian

Available Since

June 24, 2014

Availability

Academic Institutions and Nonprofits only
pcDNA3-mCherry-mCherry-FLARE-EKAR-EV

Plasmid

#123341

Purpose

Red-fluorescent homoFRET/anisotropy-based biosensor for monitoring ERK activity.

Depositor

Jin Zhang

Article

Ross et al eLife. 2018 Jul 3;7. pii: 35458. doi: 10

Insert

mCherry-mCherry-FLARE-EKAR-EV

Tags

6xHIS, T7 tag (gene 10 leader), Xpress (TM) tag, …

Expression

Mammalian

Promoter

CMV

Available Since

July 28, 2020

Availability

Academic Institutions and Nonprofits only

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pGEX6P1-HA-MEK1 CBM

Plasmid

#53163

Purpose

Expresses GST-HA tagged human MEK1 M187AH188AM230AH239A from bacterial expression vector

Depositor

Christopher Counter

Article

Brady et al Nature. 2014 Apr 9. doi: 10.1038/nature1

Insert

MEK1 (MAP2K1 Human)

Tags

GST and HA

Expression

Bacterial

Mutation

M187A/H188A/M230A/H239A

Available Since

June 9, 2014

Availability

Academic Institutions and Nonprofits only
pGEX6P1-HA-MEK5 CBM-E

Plasmid

#53168

Purpose

Expresses GST-HA tagged human MEK5 L280AH281AM323AQ332A from bacterial expression vector

Depositor

Christopher Counter

Article

Brady et al Nature. 2014 Apr 9. doi: 10.1038/nature1

Insert

MEK5 (MAP2K5 Human)

Tags

GST and HA

Expression

Bacterial

Mutation

L280A/H281A/M323A/Q332A

Available Since

Sept. 29, 2014

Availability

Academic Institutions and Nonprofits only
pWZLblasti-HA-MEK5 CBM-E

Plasmid

#53169

Purpose

Expresses HA tagged human MEK5 L280AH281AM323AQ332A from blasticidin resistance retroviral vector

Depositor

Christopher Counter

Article

Brady et al Nature. 2014 Apr 9. doi: 10.1038/nature1

Insert

MEK5 (MAP2K5 Human)

Use

Retroviral

Tags

HA

Expression

Mammalian

Mutation

L280A/H281A/M323A/Q332A

Available Since

Sept. 19, 2014

Availability

Academic Institutions and Nonprofits only
pLenti.puro.MCV.ER.RAZ.2

Plasmid

#114382

Purpose

This lentiviral plasmid expresses MCV tumor T antigens (truncated LT and small T) using the viral NCCR (non-coding control region). The sequence was amplifeied from MCV.R17a and truncated LT=428 aa.

Depositor

Reety Arora, Sudhir Krishna

Article

Arora et al Microb Pathog. 2019 Dec;137:103778. doi:

Insert

MCV NCCR and tumor T antigen regions

Use

Lentiviral

Expression

Mammalian

Promoter

MCV NCCR (viral own promoter)

Available Since

Sept. 11, 2018

Availability

Academic Institutions and Nonprofits only

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pwM

Plasmid

#22515

Depositor

Christopher Buck

Article

Pastrana et al PLoS Pathog. 2009 Sep . 5(9):e1000578.

Insert

MCPyV VP1

Expression

Mammalian

Mutation

codon modified gene

Available Since

Nov. 13, 2009

Availability

Academic Institutions and Nonprofits only

Enlarge
ph2m

Plasmid

#22518

Depositor

Christopher Buck

Article

Pastrana et al PLoS Pathog. 2009 Sep . 5(9):e1000578.

Insert

MCPyV VP2

Expression

Mammalian

Mutation

codon modified

Available Since

Nov. 13, 2009

Availability

Academic Institutions and Nonprofits only

Enlarge
pQP-CiRISB

Plasmid

#138978

Purpose

Light-controlled tri-directional targeting of endogenous proteins (cytoplasm-shifted)

Depositor

Vladislav Verkhusha

Article

Redchuk et al Nat Commun. 2020 Jan 30;11(1):605. doi:

Insert

NcoI-BphP1-CAAX-IRES2-iB(GFP)-NESmCherry- Q-PAS1-AsLOV2cNLS-NotI

Available Since

April 23, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pQP-2473

Plasmid

#138976

Purpose

Nucleus-to-cytoplasm relocalization of genomically expressed GFP-fusion using NIR light controlled intrabody.

Depositor

Vladislav Verkhusha

Article

Redchuk et al Nat Commun. 2020 Jan 30;11(1):605. doi:

Insert

NcoI-BphP1-NES-T2A- iB(GFP)-NES-mCherry-QPAS1- NLS-NotI

Available Since

April 23, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pQP-AR10

Plasmid

#138980

Purpose

Endogenous RAS regulation using light-controlled intrabody

Depositor

Vladislav Verkhusha

Article

Redchuk et al Nat Commun. 2020 Jan 30;11(1):605. doi:

Insert

BglII-BphP1-NES-T2A- iB(RAS)-NES-mCherry-QPAS1- NLS-NotI

Available Since

April 23, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pQP-NiRISB

Plasmid

#138977

Purpose

Light-controlled tri-directional targeting of endogenous proteins (nucleus-shifted)

Depositor

Vladislav Verkhusha

Article

Redchuk et al Nat Commun. 2020 Jan 30;11(1):605. doi:

Insert

NcoI-BphP1-CAAX-IRES2-NES- iB(GFP)- mCherry-Q-PAS1-AsLOV2cNLS-NotI

Available Since

April 23, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pCR.MCV.Ori339 (589)

Plasmid

#28192

Depositor

Patrick Moore

Article

Shuda et al Proc Natl Acad Sci U S A. 2008 Oct 21. 1

Insert

MCV replication region

Expression

Bacterial

Available Since

Oct. 3, 2011

Availability

Academic Institutions and Nonprofits only
pQP-iRISB

Plasmid

#138979

Purpose

Light-controlled tri-directional targeting of endogenous proteins

Depositor

Vladislav Verkhusha

Article

Redchuk et al Nat Commun. 2020 Jan 30;11(1):605. doi:

Insert

NcoI-BphP1-CAAX-IRES2-NES-SGlinkeriB( GFP)-mCherry-Q-PAS1-AsLOV2cNLS-NotI

Available Since

April 23, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pQP-2363

Plasmid

#138975

Purpose

Relocalization of genomically expressed protein of interest from cytoplasm to nucleus upon near-infrared illumination

Depositor

Vladislav Verkhusha

Article

Redchuk et al Nat Commun. 2020 Jan 30;11(1):605. doi:

Insert

NcoI-BphP1- iB(GFP) -IRES2-NES-mCherry-QPAS1- NLS-NotI

Available Since

April 23, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA6.MCV.LT339.V5.4

Plasmid

#28193

Depositor

Patrick Moore

Article

Shuda et al Proc Natl Acad Sci U S A. 2008 Oct 21. 1

Insert

MCPyV Truncated Tumor Large T antigen (399 tumor strain)

Tags

V5

Expression

Bacterial and Mammalian

Available Since

Oct. 3, 2011

Availability

Academic Institutions and Nonprofits only

Enlarge
pQP-2460

Plasmid

#138973

Purpose

Light-induced recruitment of QPAS1-mCherry to the target protein bound to membrane; interaction occurs via intrabody fused to BphP1

Depositor

Vladislav Verkhusha

Article

Redchuk et al Nat Commun. 2020 Jan 30;11(1):605. doi:

Insert

NcoI-mVenus-CAAX-IRES2-BphP1- iB(GFP)- IRES2-NES-mCherry-Q-PAS1-NotI

Available Since

April 23, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pPI544

Plasmid

#207443

Purpose

6xHis-SS-TEV-ErkB-ErkB-T176A bacterial expression vector

Depositor

Rob Kay

Article

Nichols et al Dev Cell. 2019 Feb 25;48(4):491-505.e9.

Insert

ErkB

Use

Unspecified

Mutation

T176A

Available Since

Sept. 27, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pQP-2376

Plasmid

#138974

Purpose

Light-induced recruitment of NSImb-QPAS1-mCherry to the target protein bound to membrane; interaction occurs via intrabody fused to BphP1

Depositor

Vladislav Verkhusha

Article

Redchuk et al Nat Commun. 2020 Jan 30;11(1):605. doi:

Insert

NcoI-mVenus-CAAX-IRES2-BphP1- iB(GFP)- IRES2-NSImb-NES-mCh-Q-PAS1-NotI

Available Since

April 23, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA6 MCV LTco S220A

Plasmid

#112193

Purpose

Expression of MCPyV LT mutant (Ser 220 to Ala)

Depositor

Patrick Moore

Article

Kwun et al Proc Natl Acad Sci U S A. 2017 May 16;11

Insert

MCPyV LT codon optimized

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 19, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA6 MCV LTco S239A

Plasmid

#112194

Purpose

Expression of MCPyV LT mutant (Ser 239 to Ala)

Depositor

Patrick Moore

Article

Kwun et al Proc Natl Acad Sci U S A. 2017 May 16;11

Insert

MCPyV LT codon optimized

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 19, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA6 MCV LTco S147A

Plasmid

#112192

Purpose

Expression of MCPyV LT mutant (Ser 147 to Ala)

Depositor

Patrick Moore

Article

Kwun et al Proc Natl Acad Sci U S A. 2017 May 16;11

Insert

MCPyV LT codon optimized

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 11, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
MCV-HF LTS220A/S239A

Plasmid

#112191

Purpose

Merkel cell polyomavirus molecular clone

Depositor

Patrick Moore

Article

Kwun et al Proc Natl Acad Sci U S A. 2017 May 16;11

Insert

MCV-HF LTS220A/S239A

Mutation

T to G mutations at 3313 (nt) and 3370 (nt) of fu…

Promoter

None from vector. Insert contains native promoter…

Available Since

Feb. 11, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
MCV-HF

Plasmid

#32057

Depositor

Patrick Moore

Article

Feng et al PLoS One. 2011;6(7):e22468. Epub 2011 Ju

Insert

MCV-HF genome

Promoter

none from vector. Insert contains native promoter…

Available Since

Oct. 25, 2011

Availability

Academic Institutions and Nonprofits only

Enlarge
MCV-hVam6p-

Plasmid

#32059

Depositor

Patrick Moore

Article

Feng et al PLoS One. 2011;6(7):e22468. Epub 2011 Ju

Insert

MCV-hVam6p-

Mutation

TG to GC mutations at 3280-3281 (nt) of full cons…

Promoter

none from vector. Insert contains native promoter…

Available Since

Oct. 25, 2011

Availability

Academic Institutions and Nonprofits only

Enlarge
pPI214

Plasmid

#207440

Purpose

ErkB knock out vector

Depositor

Rob Kay

Article

Nichols et al Dev Cell. 2019 Feb 25;48(4):491-505.e9.

Insert

ErkB

Use

Unspecified

Available Since

Nov. 14, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pPI486

Plasmid

#207441

Purpose

6xHis-SS-TEV-ErkB bacterial expression vector

Depositor

Rob Kay

Article

Nichols et al Dev Cell. 2019 Feb 25;48(4):491-505.e9.

Insert

ErkB

Use

Unspecified

Available Since

Sept. 27, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pPI580

Plasmid

#207445

Purpose

6xHis-SS-TEV-ErkA bacterial expression vector

Depositor

Rob Kay

Article

Nichols et al Dev Cell. 2019 Feb 25;48(4):491-505.e9.

Insert

ErkA

Use

Unspecified

Available Since

Sept. 27, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge

Showing: 481 - 520 of 2608 results

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We narrowed to 2,608 results for: ERK