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We narrowed to 1,586 results for: Tef

Showing: 601 - 650 of 1586 results

  8. pcDNA3.1-mCherry-CyrCR1.mutant

    Plasmid
    #128746
    Purpose
    Expresses mCherry in mammalian cells. ~200 nt of Cyrano with 5 point mutations in miR-7 site inserted in mCherry 3'UTR.
    Depositor
    David Bartel
    Article
    Kleaveland et al Cell. 2018 Jul 12;174(2):350-362.e17. do
    Insert
    Cyrano conserved region (CR1) with mutant miR-7 site (OIP5-AS1 Human)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    5 point mutations disrupting the miR-7 site
    Promoter
    CMV
    Available since
    Aug. 14, 2019
    Availability
    Academic Institutions and Nonprofits only
    128746-plasmid-map-sequence-id-254125

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  13. pIMVAd39T

    Plasmid
    #98303
    Purpose
    Enhancing the lower-part mevalonate pathway; overexpressing yeast mevalonate pathway genes under the control of consitutive promoters or GAL2 promoter; integrated into PDC5 locus; disrupting PDC5.
    Depositor
    Claudia Vickers
    Article
    Peng et al Biotechnol Biofuels. 2017 Feb 21;10:43.
    Insert
    pdc5(-277,-32)-PGAL2>ERG12>TNAT5-PTEF2>ERG8>TIDP1-TIDP1
    Use
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    Available since
    Aug. 7, 2017
    Availability
    Academic Institutions and Nonprofits only
    98303-plasmid-map-sequence-id-182779

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  20. pILGFPEasy9R

    Plasmid
    #218291
    Purpose
    Plasmid-free in-situ promoter cloning and characterisation system in S. cerevisiae, allows the characterisation of a bidirectional promoter using yEGFP and E2-Crimson as reporters.
    Depositor
    Claudia Vickers
    Article
    McDonnell et al bioRxiv 2024.03.26.586389
    Insert
    pURA3>KlURA3>tKlURA3-tPGK1KanMX>tAgTEF-pMAL32>MazF(RPL28i)-E2Crimson>tPDC1-pDDI2>ISceI>tSynth3>ura3(4,191)
    Use
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    Available since
    May 15, 2024
    Availability
    Academic Institutions and Nonprofits only
    218291-plasmid-map-sequence-id-433736

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  22. pWPT-mEGFP

    Plasmid
    #190606
    Purpose
    Obtained by creating a deletion inside the mCherry coding sequence in pWPT-/GCCACC-mEGFP-IRES-mCherry (Addgene #49235)
    Depositor
    Alessandro Quattrone
    Article
    Ambrosini et al Nucleic Acids Res. 2022 Oct 14;50(18):10
    Inserts
    mEGFP
    mCherry
    Use
    Lentiviral
    Tags
    Expression
    Mutation
    a deletion was created in mCherry CDS impairing i…
    Promoter
    EIF1-short
    Available since
    Oct. 24, 2022
    Availability
    Academic Institutions and Nonprofits only
    190606-plasmid-map-sequence-id-375284

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  23. pIPKAH

    Plasmid
    #185869
    Purpose
    Knocking out GPP1 and expressing a phosphoketolase pathway (Lactobacillus reuteri xfp and Methanosarcina barkeri pta)
    Depositor
    Claudia Vickers
    Article
    Hayat et al Microb Biotechnol. 2021 Nov;14(6):2627-2
    Insert
    GPP1(-220,80)> PAg.TEF1>hphMX6>TAg.TEF1-PENO2> Lr.xfp>T_TPI1-PTDH3>Mb.pta>TFBA1-GPP1(754, 1400)
    Use
    Tags
    Expression
    Yeast
    Mutation
    None
    Promoter
    Available since
    Sept. 6, 2022
    Availability
    Academic Institutions and Nonprofits only
    185869-plasmid-map-sequence-id-370814

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  24. THI ONLY pALD4 T-

    Plasmid
    #161497
    Purpose
    Non activation and non targeting control for CRISPR/RNA scaffold based transcription regulation in Pichia pastoris BB3rN_pTEF2_dCAS9(3xFLAG_SV40NLS)_pPOR1_MS2bind_VP64_SV40NLS_pGAP_THI11_T-_gRNA_MS2
    Depositor
    Brigitte Gasser, Diethard Mattanovich
    Article
    Baumschabl et al ACS Synth Biol. 2020 Dec 18;9(12):3202-3
    Inserts
    dCAS9
    MS2 (non activation control NAC)
    gRNA (Non targeting control NTC)
    Use
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    pALD4, pPOR1, and pTEF2
    Available since
    March 15, 2021
    Availability
    Academic Institutions and Nonprofits only
    161497-plasmid-map-sequence-id-326273

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  35. pAAV-hSYN-bArrestin2-TevC-P2A-TdTomato-WPRE-bGHpA

    Plasmid
    #89873
    Purpose
    expresses iTango bArrestin2-TevC component and TdTomato bicistronically for AAV production
    Depositor
    Hyungbae Kwon
    Article
    Lee et al Nat Methods. 2017 May;14(5):495-503. doi
    Insert
    bArrestin2-TevC-P2A-TdTomato
    Use
    AAV
    Tags
    TdTomato
    Expression
    Mammalian
    Mutation
    R173H in TdTomato (Please see depositor comments …
    Promoter
    Human synapsin
    Available since
    May 26, 2017
    Availability
    Academic Institutions and Nonprofits only
    89873-plasmid-map-sequence-id-177108

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  40. pG2J

    Plasmid
    #102882
    Purpose
    Expresses OsTIR1 via the ADH1 promoter, and the mGFP-AID via the TEF1 promoter in yeast. Both cassettes are integrated in HO locus. Assembly and characterization of the auxin-inducible degradation.
    Depositor
    Matthias Heinemann
    Article
    Papagiannakis et al Mol Cell. 2017 Jan 19;65(2):285-295. doi
    Inserts
    OsTIR1
    mGFP-AID
    Use
    Tags
    AID(71-114)
    Expression
    Yeast
    Mutation
    Promoter
    ADH1 and TEF1
    Available since
    Jan. 5, 2018
    Availability
    Academic Institutions and Nonprofits only
    102882-plasmid-map-sequence-id-199289

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  50. pIRGAL43C

    Plasmid
    #218278
    Purpose
    Introduction of a LowTempGAL Turbo module (GAL43C) to accelerate induction of GAL promoter induction
    Depositor
    Claudia Vickers
    Article
    Lu et al Nucleic Acids Res. 2024 May 29:gkae460.
    Insert
    fcy1(207-356)-pAgTEF1>ble>tAgTEF1-pNRG2>GAL4>tNRG2-pENO2>GAL3C(S509P)>tENO2-fcy1(454-778)
    Use
    Tags
    Expression
    Yeast
    Mutation
    GAL3 (S509P)
    Promoter
    Available since
    May 15, 2024
    Availability
    Academic Institutions and Nonprofits only
    218278-plasmid-map-sequence-id-433680

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Showing: 601 - 650 of 1586 results