We narrowed to 6,685 results for: tac

1. pTwist-SARS-CoV-2 Spike-deltaC18 K417N (AAT) L452R (CGT) T478K (AAA)

   Plasmid

   #212438

   Purpose

   Encodes SARS-CoV-2 spike K417N, L452R, T478K for pseudovirus production

   Depositor

   Alejandro Balazs

   Article

   Roederer et al medRxiv [Preprint]. 2024 Mar 7:2024.03.0

   Insert

   SARS-CoV-2 Spike K417N, L452R, T478K (S Severe acute respiratory syndrome coronavirus 2)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   K417N, L452R, T478K

   Promoter

   CAG

   Available since

   Sept. 13, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pTwist-SARS-CoV-2 Spike-deltaC18 T307I (ATA) T572I (ATC) D614G (GGT)

   Plasmid

   #212377

   Purpose

   Encodes SARS-CoV-2 spike T307I, T572I, D614G for pseudovirus production

   Depositor

   Alejandro Balazs

   Article

   Roederer et al medRxiv [Preprint]. 2024 Mar 7:2024.03.0

   Insert

   SARS-CoV-2 Spike T307I, T572I, D614G (S Severe acute respiratory syndrome coronavirus 2)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   T307I, T572I, D614G

   Promoter

   CAG

   Available since

   Sept. 13, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pTwist-SARS-CoV-2 Spike-deltaC18 D614G (GGT) Q677H (CAT) D936Y (TAT)

   Plasmid

   #212379

   Purpose

   Encodes SARS-CoV-2 spike D614G, Q677H, D936Y for pseudovirus production

   Depositor

   Alejandro Balazs

   Article

   Roederer et al medRxiv [Preprint]. 2024 Mar 7:2024.03.0

   Insert

   SARS-CoV-2 Spike D614G, Q677H, D936Y (S Severe acute respiratory syndrome coronavirus 2)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   D614G, Q677H, D936Y

   Promoter

   CAG

   Available since

   Sept. 13, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pTwist-SARS-CoV-2 Spike-deltaC18 SAK006840 VOC-501Y.V2 (B.1.351v3) - No RBD Mutations

   Plasmid

   #212380

   Purpose

   Encodes SARS-CoV-2 spike D80A, deltaL242-L244, R246I, D614G, A701V for pseudovirus production

   Depositor

   Alejandro Balazs

   Article

   Roederer et al medRxiv [Preprint]. 2024 Mar 7:2024.03.0

   Insert

   SARS-CoV-2 Spike D80A, G215D, deltaL242-L244, R246I, D614G, A701V (S Severe acute respiratory syndrome coronavirus 2)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   D80A, G215D, deltaL242-L244, R246I, D614G, A701V

   Promoter

   CAG

   Available since

   Sept. 13, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pCL051 AAVS1-9xTetO-nTATAchr21-IGKleader-Cas9site-hIgG1_FC-Myc-PDGFRb-T2A-Citrine-PolyA

   Plasmid

   #188745

   Purpose

   Donor vector for integrating nonTATAchr21 promoter-driven dual surface marker/mCitrine fluorescent reporter at AAVS1 locus

   Depositor

   Lacra Bintu, Michael Bassik

   Article

   Tycko et al Nat Biotechnol. 2024 Nov 1. doi: 10.1038

   Insert

   Surface marker and mCitrine reporter
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   nonTATAchr21

   Available since

   May 7, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pCL053 AAVS1-9xTetO-nTATAchrX-IGKleader-Cas9site-hIgG1_FC-Myc-PDGFRb-T2A-Citrine-PolyA

   Plasmid

   #188746

   Purpose

   Donor vector for integrating nonTATAchrX promoter-driven dual surface marker/mCitrine fluorescent reporter at AAVS1 locus

   Depositor

   Lacra Bintu, Michael Bassik

   Article

   Tycko et al Nat Biotechnol. 2024 Nov 1. doi: 10.1038

   Insert

   Surface marker and mCitrine reporter
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   nonTATAchrX

   Available since

   May 7, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pTwist-SARS-CoV-2 Spike-deltaC18 S371F (TTC) S373P (CCA) S375F (TTC) T376A (GCT)

   Plasmid

   #212493

   Purpose

   Encodes SARS-CoV-2 spike S371F, S373P, S375F, T376A for pseudovirus production

   Depositor

   Alejandro Balazs

   Article

   Roederer et al medRxiv [Preprint]. 2024 Mar 7:2024.03.0

   Insert

   SARS-CoV-2 Spike S371F, S373P, S375F, T376A (S Severe acute respiratory syndrome coronavirus 2)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   S371F, S373P, S375F, T376A

   Promoter

   CAG

   Available since

   Sept. 13, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pTwist-SARS-CoV-2 Spike-deltaC18 Q493R (CGA) Q498R (CGA) N501Y (TAT) Y505H (CAT)

   Plasmid

   #212498

   Purpose

   Encodes SARS-CoV-2 spike Q493R, Q498R, N501Y, Y505H for pseudovirus production

   Depositor

   Alejandro Balazs

   Article

   Roederer et al medRxiv [Preprint]. 2024 Mar 7:2024.03.0

   Insert

   SARS-CoV-2 Spike Q493R, Q498R, N501Y, Y505H (S Severe acute respiratory syndrome coronavirus 2)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Q493R, Q498R, N501Y, Y505H

   Promoter

   CAG

   Available since

   Sept. 13, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pTwist-SARS-CoV-2 Spike-deltaC18 K417N (AAT) L452R (CGT) T478K (AAA) E484K (AAA)

   Plasmid

   #212439

   Purpose

   Encodes SARS-CoV-2 spike K417N, L452R, T478K, E484K for pseudovirus production

   Depositor

   Alejandro Balazs

   Article

   Roederer et al medRxiv [Preprint]. 2024 Mar 7:2024.03.0

   Insert

   SARS-CoV-2 Spike K417N, L452R, T478K, E484K (S Severe acute respiratory syndrome coronavirus 2)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   K417N, L452R, T478K, E484K

   Promoter

   CAG

   Available since

   Sept. 13, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pTwist-SARS-CoV-2 Spike-deltaC18 Q493R (CGA) G496S (TCA) Q498R (CGA) N501Y (TAT) Y505H (CAT)

    Plasmid

    #212477

    Purpose

    Encodes SARS-CoV-2 spike Q493R, G496S, Q498R, N501Y, Y505H for pseudovirus production

    Depositor

    Alejandro Balazs

    Article

    Roederer et al medRxiv [Preprint]. 2024 Mar 7:2024.03.0

    Insert

    SARS-CoV-2 Spike Q493R, G496S, Q498R, N501Y, Y505H (S Severe acute respiratory syndrome coronavirus 2)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Q493R, G496S, Q498R, N501Y, Y505H

    Promoter

    CAG

    Available since

    Sept. 13, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pTRC-LIC

    Plasmid

    #62343

    Purpose

    Used for tac promoter regulated high-level expression of cloned, untagged genes in E. Coli.

    Depositor

    Cheryl Arrowsmith

    Type

    Empty backbone

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    tac promoter from pKK233-2

    Available since

    March 2, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. ST-2xTstem(5A)-GFP

    Plasmid

    #162504

    Purpose

    Expresses a ST6GAL1 mutant with a GFP tag in its C-terminus in mammalian cells. One wildtype Tac stem region is inserted within ST and the other mutated Tac stem region is inserted between ST and GFP.

    Depositor

    Lei Lu

    Article

    Sun et al J Biol Chem. 2020 Aug 21. pii: RA120.014

    Insert

    GFP tagged ST6Gal1 mutant (ST6GAL1, IL2RA Human)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    One Tac stem region is inserted within ST6Gal1 an…

    Promoter

    Available since

    Feb. 1, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. ST-2xTstem-GFP

    Plasmid

    #162503

    Purpose

    Expresses a ST6GAL1 mutant with a GFP tag in its C-terminus in mammalian cells. One Tac stem region is inserted within ST and the other Tac stem region is inserted between ST and GFP.

    Depositor

    Lei Lu

    Article

    Sun et al J Biol Chem. 2020 Aug 21. pii: RA120.014

    Insert

    GFP tagged ST6Gal1 mutant (ST6GAL1, IL2RA Human)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    One Tac stem region is inserted within ST6Gal1 an…

    Promoter

    Available since

    Jan. 29, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. ST-Tstem(5A)-GFP

    Plasmid

    #162502

    Purpose

    Expresses a ST6GAL1 mutant with a GFP tag in its C-terminus in mammalian cells. The mutated stem region of Tac is inserted within ST.

    Depositor

    Lei Lu

    Article

    Sun et al J Biol Chem. 2020 Aug 21. pii: RA120.014

    Insert

    ST6Gal1 mutant (the mutated Tac stem region is inserted within ST) (ST6GAL1, IL2RA Human)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    The mutated Tac stem region is inserted within ST…

    Promoter

    Available since

    Feb. 1, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. ST-Tstem-GFP

    Plasmid

    #162501

    Purpose

    Expresses a ST6GAL1 mutant with a GFP tag in its C-terminus in mammalian cells. The stem region of Tac is inserted within ST.

    Depositor

    Lei Lu

    Article

    Sun et al J Biol Chem. 2020 Aug 21. pii: RA120.014

    Insert

    ST6Gal1 mutant (Tac stem region is inserted within ST) (ST6GAL1, IL2RA Human)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    The Tac stem region is inserted within ST6Gal1

    Promoter

    Available since

    Jan. 29, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pSEVA47_Helper

    Plasmid

    #231420

    Purpose

    M13 helper genes cloned into the pSEVA631 vector. This plasmid does not carry an M13 packaging signal. (This plasmid is a gift of the Jaramillo Lab, where it is called PAJ296.)

    Depositor

    Alfonso Jaramillo

    Article

    Kushwaha et al Zenodo (2017) Conference: SB7.0: The Sev

    Insert

    gp1-gp11
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 19, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pJB37

    Plasmid

    #202602

    Purpose

    Arabinose inducible expression vector expressing retron Rcat-Sen2

    Depositor

    Athanasios Typas

    Article

    Bobonis et al Nat Protoc. 2024 May 9. doi: 10.1038/s41

    Insert

    RcaT-Sen2 toxin
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    July 20, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pSEVA63_sgRNA-B1_M13ps

    Plasmid

    #231422

    Purpose

    M13 phagemid that expresses sgRNA-B1 from a strong constitutive promoter. (This plasmid is a gift of the Jaramillo Lab, where it is called PAJ615.)

    Depositor

    Alfonso Jaramillo

    Article

    Kushwaha et al Zenodo (2017) Conference: SB7.0: The Sev

    Insert

    sgRNA-B1
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    June 3, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pSEVA47_dCas9-GFP_NOT_iC

    Plasmid

    #231418

    Purpose

    This NOT-gate plasmid expresses dCas9 from a constitutive promoter, and GFP from a promoter repressible by sgRNA-C1. (This plasmid is a gift of the Jaramillo Lab, where it is called PAJ290.)

    Depositor

    Alfonso Jaramillo

    Article

    Kushwaha et al Zenodo (2017) Conference: SB7.0: The Sev

    Inserts

    dCas9
    GFP
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    April 14, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pST

    Plasmid

    #213143

    Purpose

    TtgV Biosensor

    Depositor

    Radhakrishnan Mahadevan

    Article

    Pham et al ACS Synth Biol. 2024 Jul 19;13(7):2246-2

    Insert

    TtgV sfGFP Biosensor
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Tac

    Available since

    April 1, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pSEVA26_sgRNA-2_M13ps

    Plasmid

    #231412

    Purpose

    M13 phagemid that expresses sgRNA-2 from a strong constitutive promoter. (This plasmid is a gift of the Jaramillo Lab, where it is called PAJ171.)

    Depositor

    Alfonso Jaramillo

    Article

    Kushwaha et al Zenodo (2017) Conference: SB7.0: The Sev

    Insert

    sgRNA-2
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 19, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pSEVA47_dCas9-GFP_NOT_iA

    Plasmid

    #231417

    Purpose

    This NOT-gate plasmid expresses dCas9 from a constitutive promoter, and GFP from a promoter repressible by sgRNA-A1. (This plasmid is a gift of the Jaramillo Lab, where it is called PAJ285.)

    Depositor

    Alfonso Jaramillo

    Article

    Kushwaha et al Zenodo (2017) Conference: SB7.0: The Sev

    Inserts

    dCas9
    GFP
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 19, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pSEVA26_sgRNA-A1_M13ps

    Plasmid

    #231414

    Purpose

    M13 phagemid that expresses sgRNA-A1 from a strong constitutive promoter. (This plasmid is a gift of the Jaramillo Lab, where it is called PAJ164.)

    Depositor

    Alfonso Jaramillo

    Article

    Kushwaha et al Zenodo (2017) Conference: SB7.0: The Sev

    Insert

    sgRNA-A1
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 19, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pSEVA63_sgRNA-B0_M13ps

    Plasmid

    #231421

    Purpose

    M13 phagemid constitutively expressing sgRNA-B0. sgRNA-B0 is a dummy 20-nt sequence, containing a Golden Gate cloning site (BsaI). (A gift of the Jaramillo Lab, where it is called PAJ552.)

    Depositor

    Alfonso Jaramillo

    Article

    Kushwaha et al Zenodo (2017) Conference: SB7.0: The Sev

    Insert

    sgRNA-B0
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    June 9, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pSEVA19_sgRNA-C1_M13ps

    Plasmid

    #231411

    Purpose

    M13 phagemid that expresses sgRNA-C1 (= sgRNA A5T from Nielsen et al., 2014) from a strong constitutive promoter. (This plasmid is a gift of the Jaramillo Lab, where it is called PAJ272.)

    Depositor

    Alfonso Jaramillo

    Article

    Kushwaha et al Zenodo (2017) Conference: SB7.0: The Sev

    Insert

    sgRNA-C1
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 24, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pSEVA19_sgRNA-C0_M13ps

    Plasmid

    #231410

    Purpose

    M13 phagemid constitutively expressing sgRNA-C0. sgRNA-C0 is a dummy 20-nt sequence, containing a Golden Gate cloning site (BsaI). (A gift of the Jaramillo Lab, where it is called PAJ156.)

    Depositor

    Alfonso Jaramillo

    Article

    Kushwaha et al Zenodo (2017) Conference: SB7.0: The Sev

    Insert

    sgRNA-C0
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 19, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pAWP89

    Plasmid

    #61264

    Purpose

    IncP-based replicating vector expressing dTomato under tac promoter.

    Depositor

    Mary Lidstrom

    Article

    Puri et al Appl Environ Microbiol. 2014 Dec 29. pii

    Insert

    Ptac-dTomato
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    tac

    Available since

    Feb. 5, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pJB158

    Plasmid

    #202601

    Purpose

    RP4-based mobilizable plasmid for the IPTG inducible expression of inserts that can be introduced using BsaI-based Golden Gate cloning. Vector has two origins of replication.

    Depositor

    Athanasios Typas

    Article

    Bobonis et al Nat Protoc. 2024 May 9. doi: 10.1038/s41

    Type

    Empty backbone

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Oct. 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. DT035A

    Plasmid

    #159397

    Purpose

    Constitutive superfolder GFP (sfGFP) expression, controlled under E.coli tac promoter

    Depositor

    Sheref Mansy

    Article

    Toparlak et al Sci Adv. 2020 Sep 18;6(38). pii: 6/38/ea

    Insert

    pr.tac-sfGFP
    

    Use

    Synthetic Biology

    Tags

    FLAG

    Expression

    Bacterial

    Mutation

    Promoter

    tac promoter

    Available since

    March 15, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. Endophilin II Full

    Plasmid

    #47409

    Depositor

    Peter McPherson

    Insert

    Endophilin II
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    Promoter

    tac

    Available since

    Aug. 9, 2018

    Availability

    Academic Institutions and Nonprofits only

31. Endophilin III Full

    Plasmid

    #47410

    Depositor

    Peter McPherson

    Insert

    Endophilin III
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    Promoter

    tac

    Available since

    Aug. 9, 2018

    Availability

    Academic Institutions and Nonprofits only

32. NSSCP

    Plasmid

    #183190

    Purpose

    mRFP-PopZ expressed under J23116 promoter; split N terminal of TEV protease-SpmXΔC expressed under Tac promoter; SpmXΔC-split C terminal of TEV protease expressed under Tac promoter; DivIVA-sfGFP-TEV

    Depositor

    Hsiao-Chun Huang

    Article

    Hong et al ACS Synth Biol. 2021 Aug 20;10(8):1830-1

    Inserts

    mRFP-PopZ
    DivIVA-GFP(tevR-50)
    SpmX-TEV(C)
    TEV(N)-SpmX
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    July 12, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pDA03

    Plasmid

    #65943

    Purpose

    Theophylline riboswitch D derivatives 03

    Depositor

    Justin Gallivan

    Insert

    luciferase
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Tac

    Available since

    Sept. 3, 2015

    Availability

    Academic Institutions and Nonprofits only

34. pDA08

    Plasmid

    #65944

    Purpose

    Theophylline riboswitch D derivatives 08

    Depositor

    Justin Gallivan

    Insert

    luciferase
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Tac

    Available since

    Sept. 3, 2015

    Availability

    Academic Institutions and Nonprofits only

35. pTMR-S02

    Plasmid

    #65941

    Purpose

    TMR Riboswitch S02 with luciferase gene

    Depositor

    Justin Gallivan

    Insert

    luciferase
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Tac

    Available since

    Sept. 3, 2015

    Availability

    Academic Institutions and Nonprofits only

36. DT033A

    Plasmid

    #159394

    Purpose

    Constitutive murine mature BDNF expression, controlled under E.coli tac promoter

    Depositor

    Sheref Mansy

    Article

    Toparlak et al Sci Adv. 2020 Sep 18;6(38). pii: 6/38/ea

    Insert

    pr.tac-BDNF (Bdnf, Mouse) (Bdnf Mouse)
    

    Use

    Synthetic Biology

    Tags

    FLAG

    Expression

    Bacterial

    Mutation

    Promoter

    tac promoter

    Available since

    Feb. 10, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pMAL-p5X

    Plasmid

    #150814

    Purpose

    Expresses proteins in the periplasm as fusions to a high-binding mutant (A313V) maltose-binding protein

    Depositor

    Paul Riggs

    Type

    Empty backbone

    Use

    Tags

    malE A313V

    Expression

    Bacterial

    Mutation

    Promoter

    tac

    Available since

    July 22, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

38. pOpen_taq

    Plasmid

    #153315

    Purpose

    Bacterial expression of Taq polymerase

    Depositor

    Drew Endy

    Insert

    Taq polymerase
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Tac

    Available since

    June 2, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

39. pGEX-2T-TRR-C421-Y2404F

    Plasmid

    #182846

    Purpose

    mutation Y2404F (TAC/TTC) in GST-TRR-C421 (TRR 2011-2431 aa). Expression in E. coli. by IPTG induction

    Depositor

    Peter Harte

    Article

    Tie et al Development. 2014 Mar;141(5):1129-39. do

    Insert

    TRR 2011-2431 (trr Fly)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    mutation Y2404F (TAC/TTC) in GST-TRR-C421 (TRR 20…

    Promoter

    Available since

    April 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pHN764

    Plasmid

    #178819

    Purpose

    Expression of EndoH in E. coli

    Depositor

    Kazuhiro Abe

    Insert

    synthetic gene encoding endo-β-N-acetylglucosaminidase H
    

    Use

    Tags

    HIs8 and MBP

    Expression

    Bacterial

    Mutation

    Promoter

    tac

    Available since

    Jan. 12, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

41. pGTvL1-SGC

    Plasmid

    #39188

    Purpose

    SGC Empty backbone for bacterial expression

    Depositor

    Nicola Burgess-Brown

    Type

    Empty backbone

    Use

    Tags

    GST and TEV cleavage site

    Expression

    Bacterial

    Mutation

    Promoter

    Tac (lactose/IPTG inducible)

    Available since

    March 18, 2013

    Availability

    Academic Institutions and Nonprofits only

42. pHH0103 genomicseq WW domain

    Plasmid

    #104299

    Purpose

    Control for WW domain - genomicseq

    Depositor

    Sachdev Sidhu, Marius Sudol

    Insert

    genomicseq
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    codon optimized for expression in bacteria

    Promoter

    tac

    Available since

    Aug. 28, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

43. pHN922

    Plasmid

    #178975

    Purpose

    Expression of GST-His8-GFP-nanobody

    Depositor

    Kazuhiro Abe

    Insert

    GST-His8-GFP-nanobody
    

    Use

    Affinity Reagent/ Antibody

    Tags

    GST, His8

    Expression

    Bacterial

    Mutation

    Promoter

    tac

    Available since

    Feb. 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

44. pAG GST EGFP

    Plasmid

    #236736

    Purpose

    Bacterial expression plasmid containing GST-tagged EGFP

    Depositor

    Addgene Research Program, Melina Fan

    Insert

    EGFP
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    Promoter

    Tac

    Available since

    April 14, 2025

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

45. P61m

    Plasmid

    #120953

    Purpose

    MoClo golden gate assembly AB part for pTac (Adam Meyer's improved pTac promoter selected for dynamic range and low cross-sensitivity; unpublished, using strategy from https://doi.org/10.1101/285866).

    Depositor

    Richard Murray

    Insert

    Tac promoter Adam Meyer
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Dec. 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

46. pAraTM

    Plasmid

    #39547

    Depositor

    Bryan Berger

    Article

    Su et al J Biol Chem. 2012 Jul 22.

    Type

    Empty backbone

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Tac

    Available since

    Aug. 29, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

47. pBlu-CGT-1

    Plasmid

    #170158

    Purpose

    Expresses bacterial Cyclomaltodextrin Glucanotransferase from Paenibacillus sp. c36 in E.coli

    Depositor

    Sarah MacDonald

    Insert

    Cyclomaltodextrin Glucanotransferase
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Tac

    Available since

    May 10, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

48. pHH0103 STXBP4 WW domain

    Plasmid

    #104265

    Purpose

    Bacterial expression of WW domain from STXBP4

    Depositor

    Sachdev Sidhu, Marius Sudol

    Insert

    STXBP4 WW domain (STXBP4 Human)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    codon optimized for expression in bacteria

    Promoter

    tac

    Available since

    Aug. 28, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

49. pHH0103 PIN1L WW domain

    Plasmid

    #104269

    Purpose

    Bacterial expression of WW domain from PIN1L

    Depositor

    Sachdev Sidhu, Marius Sudol

    Insert

    PIN1L WW domain (PIN1P1 Human)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    codon optimized for expression in bacteria

    Promoter

    tac

    Available since

    Aug. 28, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

50. pHH0103 NEDD4 WW domain #3

    Plasmid

    #104210

    Purpose

    Bacterial expression of WW domain #3 from NEDD4

    Depositor

    Sachdev Sidhu, Marius Sudol

    Insert

    NEDD4 WW domain #3 (NEDD4 Human)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    codon optimized for expression in bacteria

    Promoter

    tac

    Available since

    Feb. 21, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge