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Showing: 241 - 260 of 350 results

  1. pX330_ATP1A1_G7_Dual_sgRNA

    Plasmid
    #173206
    Purpose
    Coselection for HDR in human cells. Vector for dual expression of ATP1A1 G7 sgRNA in combination with a user-specified sgRNA from two independent U6 promoters. Cloning of oligos using BbsI sites.
    Depositor
    Yannick Doyon
    Article
    Levesque et al Nat Commun. 2022 Oct 7;13(1):5909. doi:
    Insert
    ATP1A1 G7 sgRNA + user-specified sgRNA + SpCas9
    Use
    CRISPR
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Dual U6 promoters
    Available since
    Nov. 3, 2021
    Availability
    Academic Institutions and Nonprofits only
    173206-plasmid-map-sequence-id-339514

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  3. pY036_ATP1A1_G5

    Plasmid
    #86618
    Purpose
    Expresses the ATP1A1 G5 crRNA in combination with AsCpf1-3xHA to target ATP1A1 intron 4. U6-crRNA(ATP1A1 G5)-CBh-AsCpf1
    Depositor
    Yannick Doyon
    Article
    Agudelo et al Nat Methods. 2017 Apr 17. doi: 10.1038/n
    Insert
    ATP1A1 G5 crRNA + AsCpf1-3xHA
    Use
    CRISPR; Co-selection via hdr using ouabain
    Tags
    3xHA
    Expression
    Mammalian
    Mutation
    Promoter
    CBh
    Available since
    March 16, 2017
    Availability
    Academic Institutions and Nonprofits only
    86618-plasmid-map-sequence-id-168853

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  4. pX330_ATP1A1_G3_Dual_sgRNA

    Plasmid
    #173205
    Purpose
    Coselection for HDR in human cells. Vector for dual expression of ATP1A1 G3 sgRNA in combination with a user-specified sgRNA from two independent U6 promoters. Cloning of oligos using BbsI sites.
    Depositor
    Yannick Doyon
    Article
    Levesque et al Nat Commun. 2022 Oct 7;13(1):5909. doi:
    Insert
    ATP1A1 G3 sgRNA + user-specified sgRNA + SpCas9
    Use
    CRISPR
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Dual U6 promoters
    Available since
    Nov. 3, 2021
    Availability
    Academic Institutions and Nonprofits only
    173205-plasmid-map-sequence-id-339509

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  5. pUC19-π-EB1_IRES-mCherry

    Plasmid
    #197427
    Purpose
    Homology-directed repair template targeting IRES-based π-element to MAPRE1 exon 5
    Depositor
    Torsten Wittmann
    Article
    Dema et al Elife. 2023 Jan 30;12:e84143. doi: 10.75
    Insert
    IRES-based π-element with HDR templates flanking MAPRE1 exon 5 insertion site (MAPRE1 Human)
    Use
    CRISPR and Synthetic Biology
    Tags
    GCN4 leucine zipper, LOV2, Zdk1, and mCherry
    Expression
    Mutation
    Promoter
    Available since
    June 26, 2023
    Availability
    Academic Institutions and Nonprofits only
    197427-plasmid-map-sequence-id-388174

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  6. pUC19-π-EB1_EF1α-mCherry

    Plasmid
    #197429
    Purpose
    Homology-directed repair template targeting EF1α-based π-element to MAPRE1 exon 5
    Depositor
    Torsten Wittmann
    Article
    Dema et al Elife. 2023 Jan 30;12:e84143. doi: 10.75
    Insert
    EF1α-based π-element with HDR templates flanking MAPRE1 exon 5 insertion site (MAPRE1 Human)
    Use
    CRISPR and Synthetic Biology
    Tags
    GCN4 leucine zipper, LOV2, Zdk1, and mCherry
    Expression
    Mutation
    Promoter
    Available since
    June 26, 2023
    Availability
    Academic Institutions and Nonprofits only
    197429-plasmid-map-sequence-id-388078

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  7. pUC19-π-EB1_EF1α-EGFP

    Plasmid
    #197430
    Purpose
    Homology-directed repair template targeting EF1α-based π-element to MAPRE1 exon 5
    Depositor
    Torsten Wittmann
    Article
    Dema et al Elife. 2023 Jan 30;12:e84143. doi: 10.75
    Insert
    EF1α-based π-element with HDR templates flanking MAPRE1 exon 5 insertion site (MAPRE1 Human)
    Use
    CRISPR and Synthetic Biology
    Tags
    EGFP, GCN4 leucine zipper, LOV2, and Zdk1
    Expression
    Mutation
    Promoter
    Available since
    June 26, 2023
    Availability
    Academic Institutions and Nonprofits only
    197430-plasmid-map-sequence-id-388175

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  14. pY036_ATP1A1_G5_Array

    Plasmid
    #86620
    Purpose
    Vector for expression of a CRISPR/AsCpf1 array containing the ATP1A1 G5 guide in combination with a user-specified guide. Cloning of oligos for the second guide using BbsI sites. PY036-like plasmid
    Depositor
    Yannick Doyon
    Article
    Agudelo et al Nat Methods. 2017 Apr 17. doi: 10.1038/n
    Insert
    ATP1A1 G5 crRNA + user-specified crRNA + AsCpf1-3xHA
    Use
    CRISPR; Co-selection via hdr using ouabain
    Tags
    3xHA
    Expression
    Mammalian
    Mutation
    Promoter
    CBh
    Available since
    March 16, 2017
    Availability
    Academic Institutions and Nonprofits only
    86620-plasmid-map-sequence-id-168857

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  17. eSpCas9(1.1)_No_FLAG_ATP1A1_G3

    Plasmid
    #86611
    Purpose
    Expresses the ATP1A1 G3 sgRNA in combination with FLAGless eSpCas9(1.1) to target ATP1A1 intron 4. Px330-like plasmid
    Depositor
    Yannick Doyon
    Article
    Agudelo et al Nat Methods. 2017 Apr 17. doi: 10.1038/n
    Insert
    ATP1A1 G3 sgRNA + FLAGless enhanced specificity Cas9 (1.1)
    Use
    CRISPR; Co-selection via hdr using ouabain
    Tags
    Expression
    Mammalian
    Mutation
    K848A, K1003A, & R1060A
    Promoter
    CBh
    Available since
    March 30, 2017
    Availability
    Academic Institutions and Nonprofits only
    86611-plasmid-map-sequence-id-168847

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Showing: 241 - 260 of 350 results