We narrowed to 52,624 results for: SON

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8xNFAT-ZsG-muhCD4

Plasmid

#162745

Purpose

NFAT-driven ZsGreen-1 reporter gene with mutated human CD4 gene

Depositor

Maki Nakayama

Article

Landry et al Front Endocrinol (Lausanne). 2021 Mar 25

Insert

8x of NFAT binding motif follwed by ZsGreen-1 fluorescent protein gene (CD4 Human)

Use

Retroviral

Expression

Mammalian

Mutation

Human CD4 gene with glutamine to tyrosine at posi…

Available Since

April 27, 2021

Availability

Academic Institutions and Nonprofits only

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GFP-BICD2

Plasmid

#164626

Purpose

Expresses BICD2 in mammalian cells with a GFP tag

Depositor

Mark Cookson

Article

Bonet-Ponce et al Sci Adv. 2020 Nov 11;6(46). pii: 6/46/ea

Insert

BICD2 (BICD2 Human)

Tags

Cycle 3 GFP

Expression

Mammalian

Promoter

CMV

Available Since

July 9, 2021

Availability

Academic Institutions and Nonprofits only

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pMB1_MmPylRS_MmtRNA-Pyl-opt(UGA)

Plasmid

#174515

Purpose

Recoded single aaRS/tRNA plasmid with MmPylRS and MmtRNA-Pyl-opt with the ASL from the serT gene (UGA anticodon)

Depositor

Jason W Chin

Article

Robertson et al Science. 2021 Jun 4;372(6546):1057-1062.

Inserts

Mm PylS
MmtRNA-Pyl-opt(UGA)

Expression

Bacterial

Mutation

ASL from the serT gene (UGA anticodon) and recoded

Promoter

GlnS and lpp

Available Since

Oct. 18, 2021

Availability

Academic Institutions and Nonprofits only

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pCAG mFoxg1-GFP wildtype

Plasmid

#73048

Purpose

C-terminal fusion of gfp Mouse wildtype Foxg1

Depositor

Joseph Gleeson

Article

Baek et al Nat Med. 2015 Dec;21(12):1445-54. doi: 1

Insert

mFoxg1 (Foxg1 Mouse)

Expression

Mammalian

Available Since

March 11, 2016

Availability

Academic Institutions and Nonprofits only

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Talin-CS

Plasmid

#83377

Purpose

Control FRET probe for measuring tension on Talin molecule

Depositor

Martin Schwartz

Article

Kumar et al J Cell Biol. 2016 May 9;213(3):371-83. d

Insert

Talin1 (Tln1 Mouse)

Use

Retroviral

Tags

EGFP-F40-tagRFP (tension sensor module)

Expression

Mammalian

Mutation

Tension sensor module is inserted at C-terminal o…

Promoter

CMV

Available Since

Oct. 26, 2016

Availability

Academic Institutions and Nonprofits only

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pCIG AKT3 S472A

Plasmid

#73052

Purpose

Human AKT3 S472A

Depositor

Joseph Gleeson

Article

Baek et al Nat Med. 2015 Dec;21(12):1445-54. doi: 1

Insert

AKT3 S472A (AKT3 Human)

Expression

Mammalian

Mutation

S472A

Available Since

March 18, 2016

Availability

Academic Institutions and Nonprofits only

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GFP-JIP3

Plasmid

#164624

Purpose

Expresses JIP3 with a GFP tag

Depositor

Mark Cookson

Article

Bonet-Ponce et al Sci Adv. 2020 Nov 11;6(46). pii: 6/46/ea

Insert

JIP3 (MAPK8IP3 Human)

Tags

cycle 3 GFP

Expression

Mammalian

Promoter

CMV

Available Since

April 21, 2021

Availability

Academic Institutions and Nonprofits only

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iBE4max YE1 NG CLYBL

Plasmid

#174570

Purpose

doxycycline inducible BE4max YE1 NG expression for human CLYBL locus knock-in

Depositor

Mathew Garnett

Article

Coelho et al Cancer Cell. 2023 Feb 13;41(2):288-303.e

Insert

BE3

Expression

Mammalian

Promoter

tet ON

Available Since

Jan. 19, 2023

Availability

Academic Institutions and Nonprofits only

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pCMV-SpRYc-ABE8e-EGFP

Plasmid

#208340

Purpose

Expresses SpRYc-ABE8e in mammalian cells

Depositor

Pranam Chatterjee

Article

Zhao et al Nat Commun. 2023 Oct 4;14(1):6175. doi:

Insert

ecTadA(8e)-nSpRYc

Use

CRISPR

Expression

Mammalian

Mutation

Sc++ (1-1119), SpRY (1111-1368), D10A

Promoter

CMV

Available Since

Aug. 20, 2024

Availability

Academic Institutions and Nonprofits only

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GFP-RAB35

Plasmid

#164630

Purpose

Expresses RAB35 in mammalian cells with a GFP tag

Depositor

Mark Cookson

Article

Bonet-Ponce et al Sci Adv. 2020 Nov 11;6(46). pii: 6/46/ea

Insert

RAB35 (RAB35 Human)

Tags

cycle 3 GFP

Expression

Mammalian

Mutation

none

Promoter

CMV

Available Since

May 22, 2021

Availability

Academic Institutions and Nonprofits only

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pLCKOv3

Plasmid

#209046

Purpose

Lentiviral backbone for expressing U6 driven SpCas9 sgRNAs with BveI cloning sites, SpCas9 tracrRNAv1 (Dang et al., 2015; PMID: 26671237) and puromycin selection marker

Depositor

Thomas Gonatopoulos-Pournatzis

Article

Xiao et al Mol Cell. 2024 Jul 11;84(13):2553-2572.e

Type

Empty backbone

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

June 20, 2024

Availability

Academic Institutions and Nonprofits only

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3xFLAG-FKBP-LRRK2

Plasmid

#193584

Purpose

Expresses human full-length LRRK2 with FKBP domain and 3xFLAG situated at the N-terminus

Depositor

Mark Cookson

Article

Kluss et al Proc Natl Acad Sci U S A. 2022 Oct 25;11

Insert

Leucine-rich repeat kinase 2 (LRRK2 Human)

Tags

3xFLAG and FKBP

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 17, 2023

Availability

Academic Institutions and Nonprofits only

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pLenti-CR033

Plasmid

#234042

Purpose

expresses ABE8e(V106W) base editor in a human T cell optimized lentiviral transfer plasmid

Depositor

Alexander Marson

Article

Schmidt et al Nature. 2024 Jan;625(7996):805-812. doi:

Insert

TadA8e(V106W)-Cas9(D10A)-P2A-blast

Use

Lentiviral

Available Since

April 4, 2025

Availability

Academic Institutions and Nonprofits only

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pCIG AKT3 E17K

Plasmid

#73050

Purpose

Human AKT3 E17K

Depositor

Joseph Gleeson

Article

Baek et al Nat Med. 2015 Dec;21(12):1445-54. doi: 1

Insert

AKT3 E17K (AKT3 Human)

Expression

Mammalian

Mutation

E17K

Available Since

April 11, 2016

Availability

Academic Institutions and Nonprofits only

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GFP-MUTED

Plasmid

#164629

Purpose

Expresses MUTED in mammalian cells with a GFP tag

Depositor

Mark Cookson

Article

Bonet-Ponce et al Sci Adv. 2020 Nov 11;6(46). pii: 6/46/ea

Insert

MUTED (BLOC1S5 Human)

Tags

cycle 3 GFP

Expression

Mammalian

Promoter

CMV

Available Since

June 12, 2021

Availability

Academic Institutions and Nonprofits only

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pEGFPN1-N-gamma-tubulin (334-449)

Plasmid

#87859

Purpose

Fluorescent fragment of gamma-tubulin containing gamma-tubulin DNA binding domain and NLS

Depositor

Maria Alvarado-Kristensson

Article

Hoog et al FASEB J. 2011 Nov;25(11):3815-27. doi: 1

Insert

gamma-tubulin 1 (TUBG1 Human)

Tags

GFP

Expression

Mammalian

Mutation

fragment from aa 334 to 449

Available Since

May 12, 2017

Availability

Academic Institutions and Nonprofits only

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pcDNA5-FRT-TO-Nsp13_GC3opt

Plasmid

#157714

Purpose

mammalian expression of untagged SARS-CoV-2 Nsp13 under control of a tetracycline-inducible promoter

Depositor

David Tollervey

Article

Bresson et al Wellcome Open Res. 2020 Nov 3;5:261. doi

Insert

Nsp13-HF_GC3opt (ORF1ab SARS-CoV-2)

Expression

Mammalian

Mutation

codon optimized; gene expression was optimized by…

Available Since

Aug. 6, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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pcDNA5-FRT-TO-FH-Nsp13_GC3opt

Plasmid

#157715

Purpose

mammalian expression of N-terminally Flag-His8 tagged SARS-CoV-2 Nsp13 under control of a tetracycline-inducible promoter

Depositor

David Tollervey

Article

Bresson et al Wellcome Open Res. 2020 Nov 3;5:261. doi

Insert

FH-Nsp13_GC3opt (ORF1ab SARS-CoV-2)

Tags

Flag-His8

Expression

Mammalian

Mutation

codon optimized; gene expression was optimized by…

Available Since

Aug. 6, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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pETM33_CALM1_EF-hand

Plasmid

#178468

Purpose

Bacterial expression of human domain with His-tag and GST tag

Depositor

Ylva Ivarsson

Article

Benz et al Mol Syst Biol. 2022 Jan;18(1):e10584. do

Insert

CALM1_EF-hand (CALM1 Human, Synthetic)

Tags

His-GST

Expression

Bacterial

Promoter

T7/LacO

Available Since

March 22, 2022

Availability

Academic Institutions and Nonprofits only

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pAAV Syn hBE Rh Guanylyl Cyclase1 2A tDimer

Plasmid

#66779

Purpose

The rhodopsin-guanylyl cyclase of the aquatic fungus Blastocladiella emersonii enables fast optical control of cGMP signaling

Depositor

Thomas Oertner

Article

Scheib et al Sci Signal. 2015 Aug 11;8(389):rs8. doi:

Inserts

hbeRhGC
red fluorescent protein

Use

AAV

Expression

Mammalian

Promoter

human Synapsin

Available Since

Oct. 16, 2015

Availability

Academic Institutions and Nonprofits only

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