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pAH_DEST_nHalo-Sec61β-3XFLAG

Plasmid

#250996

Purpose

Expression of Sec61β with N-terminal Halo and C-terminal 3XFLAG tags

Depositor

Anthony Pedley

Article

Sha et al Proc Natl Acad Sci U S A. 2026 Feb 3;123

Insert

SEC61β (SEC61B Human)

Tags

3XFLAG and Halo Tag

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 29, 2026

Availability

Academic Institutions and Nonprofits only

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pHAGE-dCas9-3XGFP-CIBN

Plasmid

#249581

Purpose

Lentiviral vector for stable expression of dCas9-3XGFP-CIBN fusion protein in mammalian cells

Depositor

Tom Misteli

Article

Stortz et al bioRxiv 2025.11.06.686574

Inserts

CIBN (CIB1 Mustard Weed)
Sp dCas9

Use

Lentiviral

Tags

3XsfGFP

Expression

Mammalian

Mutation

Catalytically dead mutant Cas9 and N-terminal por…

Promoter

CMV

Available Since

Jan. 20, 2026

Availability

Academic Institutions and Nonprofits only

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pQβEP1

Plasmid

#210859

Purpose

Express phage Qβ with SARS-CoV Spike protein epitope 1 displayed on the surface that can bind anti-S antibodies under T7 promotor

Depositor

Alain Bopda Waffo

Article

Sanders et al J Biol Chem. 2023 Dec;299(12):105460. do

Insert

SARS-CoV Spike AA441-460 (S )

Tags

No

Expression

Bacterial

Promoter

T7

Available Since

Jan. 7, 2026

Availability

Academic Institutions and Nonprofits only

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pQβEP3

Plasmid

#210861

Purpose

Express phage Qβ with SARS-CoV Spike protein epitope 3 displayed on the surface that can bind anti-S antibodies under T7 promotor

Depositor

Alain Bopda Waffo

Article

Sanders et al J Biol Chem. 2023 Dec;299(12):105460. do

Insert

SARS-CoV Spike AA781-800 (S )

Tags

No

Expression

Bacterial

Promoter

T7

Available Since

Jan. 7, 2026

Availability

Academic Institutions and Nonprofits only

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TPC2-G-GECO1.2 (F90L)

Plasmid

#240470

Purpose

Lysosomal channel with a low-affinity GECI fused to the C-terminus

Depositor

Antony Galione

Article

Davis et al Cell Calcium. 2023 Dec;116:102801. doi:

Insert

TPC2N2 (TPCN2 Human)

Tags

G-GECO1.2_F90L

Expression

Mammalian

Available Since

Dec. 4, 2025

Availability

Academic Institutions and Nonprofits only

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TPC2-D276K-mCherry

Plasmid

#240469

Purpose

Red-tagged lysosomal channel (pore-dead)

Depositor

Antony Galione

Article

Davis et al Cell Calcium. 2023 Dec;116:102801. doi:

Insert

TPC2N2 (TPCN2 Human)

Tags

mCherry

Expression

Mammalian

Mutation

Mutated aspartate 276 to lysine.

Promoter

CMV

Available Since

Nov. 18, 2025

Availability

Academic Institutions and Nonprofits only

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TPC2-RCaMP181

Plasmid

#240466

Purpose

Lysosomal channel with a red, mid-affinity GECI fused to the C-terminus

Depositor

Antony Galione

Article

Davis et al Cell Calcium. 2023 Dec;116:102801. doi:

Insert

TPC2N2 (TPCN2 Human)

Tags

RCaMP181 (a.k.a. RCaMP1h)

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 23, 2025

Availability

Academic Institutions and Nonprofits only

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TPC2-GCaMP6s

Plasmid

#240465

Purpose

Lysosomal channel with a high-affinity GECI fused to the C-terminus

Depositor

Antony Galione

Article

Davis et al Cell Calcium. 2023 Dec;116:102801. doi:

Insert

TPC2N2 (TPCN2 Human)

Tags

GCaMP6s

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pSmBit-CHIP-G132N

Plasmid

#236065

Purpose

SmBit-CHIP expression with G132N substitution

Depositor

Jonathan Schisler

Article

Stewart et al bioRxiv 2025.02.17.637997

Insert

SmBit-CHIP-G132N (STUB1 Human)

Use

Luciferase

Tags

SmBit

Mutation

G132N

Promoter

HSV-TK

Available Since

Sept. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pSmBit-CHIP-K30A

Plasmid

#236066

Purpose

SmBit-CHIP expression with K30A substitution

Depositor

Jonathan Schisler

Article

Stewart et al bioRxiv 2025.02.17.637997

Insert

SmBit-CHIP-K30A (STUB1 Human)

Use

Luciferase

Tags

SmBit

Mutation

K30A

Promoter

HSV-TK

Available Since

Sept. 5, 2025

Availability

Academic Institutions and Nonprofits only

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pCS2+8-CYP2A6(C82A)-flag-tev-his

Plasmid

#227166

Purpose

For overexpression of CYP2A6 C82A mutant in mammalian cells and purification.

Depositor

Yimon Aye

Article

Liu et al Cell. 2024 Dec 26;187(26):7450-7469.e29.

Insert

CYP2A6 (CYP2A6 Human)

Tags

Flag and His6

Expression

Mammalian

Mutation

changed cysteine 82 to alanine

Promoter

CMV and SP6

Available Since

Aug. 19, 2025

Availability

Academic Institutions and Nonprofits only

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pCS2+8-cyp-33e1-flag-tev-halo-his

Plasmid

#227151

Purpose

For T-REX experiments of cyp-33e1. A reactive electrophilic species (RES) Sensor.

Depositor

Yimon Aye

Article

Liu et al Cell. 2024 Dec 26;187(26):7450-7469.e29.

Insert

cyp-33e1 (cyp-33E1 Nematode)

Tags

Flag, HaloTag, and His6

Expression

Mammalian

Promoter

CMV and SP6

Available Since

Aug. 19, 2025

Availability

Academic Institutions and Nonprofits only

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pCS2+8-CYP2A6-flag-tev-halo-his

Plasmid

#227154

Purpose

For T-REX experiments of CYP2A6. A reactive electrophilic species (RES) Sensor.

Depositor

Yimon Aye

Article

Liu et al Cell. 2024 Dec 26;187(26):7450-7469.e29.

Insert

CYP2A6 (CYP2A6 Human)

Tags

Flag, HaloTag, and His6

Expression

Mammalian

Promoter

CMV and SP6

Available Since

Aug. 19, 2025

Availability

Academic Institutions and Nonprofits only

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Gab2-MSCV-IRES-GFP

Plasmid

#240126

Purpose

Expresses Gab2 (mouse) in mammalian cells

Depositor

Timothy Ley

Article

Kramer et al J Clin Invest. 2025 Aug 7:e195929. doi:

Insert

Gab2 (Gab2 Mouse)

Use

Retroviral

Tags

IRES-GFP

Expression

Mammalian

Available Since

Aug. 7, 2025

Availability

Academic Institutions and Nonprofits only

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YFP-cCGNL1-Myc

Plasmid

#236513

Purpose

For expression of Paracingulin (dog) in mammalian cells

Depositor

Sandra Citi

Article

Paschoud et al Mol Membr Biol. 2008 Jan;25(1):1-13. doi

Insert

CGNL1 (CGNL1 Canis lupus familiaris (dog))

Tags

Myc and YFP

Expression

Mammalian

Mutation

WT

Available Since

April 24, 2025

Availability

Academic Institutions and Nonprofits only

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GFP-mCGN

Plasmid

#236511

Purpose

For expression of GFP-Cingulin (mouse) in mammalian cells

Depositor

Sandra Citi

Article

Flinois et al J Cell Sci. 2024 Mar 1;137(5):jcs260745.

Insert

CGN (Cgn Mouse)

Tags

GFP

Expression

Mammalian

Mutation

WT

Available Since

April 16, 2025

Availability

Academic Institutions and Nonprofits only

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cCGNL1-HA

Plasmid

#236518

Purpose

For expression of Paracingulin (dog) in mammalian cells

Depositor

Sandra Citi

Article

Flinois et al J Cell Sci. 2024 Mar 1;137(5):jcs260745.

Insert

CGNL1 (CGNL1 Canis lupus familiaris (dog))

Tags

HA

Expression

Mammalian

Mutation

WT

Available Since

April 16, 2025

Availability

Academic Institutions and Nonprofits only

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JDW 1392 (pLenti-CMV-FOXF1-P2A-H2A-mCherry)

Plasmid

#233546

Purpose

A CMV driven human FOXF1 followed by a P2A cleavage peptide and an H2A mCherry fusion protein.

Depositor

Joshua Wythe

Article

Cantu Gutierrez et al bioRxiv 2021.11.15.468651

Insert

FOXF1 (FOXF1 Human)

Use

Lentiviral

Tags

FLAG

Available Since

March 20, 2025

Availability

Academic Institutions and Nonprofits only

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pAG415Gal_Gcn4 SATtoG KtoRHA

Plasmid

#232994

Purpose

Galactose iduced expression of Gcn4 SATtoG KtoRHAin yeast

Depositor

Aseem Ansari, Tanja Mittag

Article

Bremer et al bioRxiv [Preprint]. 2024 Nov 22:2024.11.

Insert

Gcn4 SATtoG KtoR

Tags

3xHA and TEV cleavage site

Expression

Yeast

Mutation

S101G, T102G, S104G, T105G, S117G, K118R, T121G, …

Promoter

Gal1

Available Since

March 20, 2025

Availability

Academic Institutions and Nonprofits only

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pAG415Gal_Gcn4 SATtoG KtoR

Plasmid

#232961

Purpose

Galactose iduced expression of Gcn4 ATtoG KtoR in yeast

Depositor

Aseem Ansari, Tanja Mittag

Article

Bremer et al bioRxiv [Preprint]. 2024 Nov 22:2024.11.

Insert

Gcn4 SATtoG KtoR

Tags

TEV cleavage site

Expression

Yeast

Mutation

S101G, T102G, S104G, T105G, S117G, K118R, T121G, …

Promoter

Gal1

Available Since

March 20, 2025

Availability

Academic Institutions and Nonprofits only

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