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pAAV-syn-jYCaMP1s

Plasmid

#135424

Purpose

Yellow protein calcium sensor expressed under human Synapsin1 promoter, for AAV production or direct transfection, slow variant

Depositor

Kaspar Podgorski

Publication

Mohr et al Nat Methods. 2020 May 25. pii: 10.1038/s

Has service

AAV1

Insert

jYCaMP1s

Use

AAV

Expression

Mammalian

Promoter

hSyn

Available since

Jan. 8, 2020

Availability

Academic Institutions and Nonprofits only

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medSIIN

Plasmid

#185664

Purpose

shApc-expressing lentiviruses with varius levels of expression of SIINFEKL

Depositor

Tyler Jacks

Publication

Westcott et al Nat Cancer. 2021 Oct;2(10):1071-1085. do

Inserts

mScarlet
miR-30a
shApc

Use

Lentiviral

Promoter

Ef1a

Available since

Nov. 4, 2022

Availability

Academic Institutions and Nonprofits only

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pAAV-CAG-2xNLS-tdTomato-WPRE-hGHpolyA

Plasmid

#192552

Purpose

Expresses nuclear localized tdTomato under CAG promoter

Depositor

Guoping Feng

Has service

AAV9 and AAV PHP.eB

Insert

tdTomato

Use

AAV

Tags

NLS

Mutation

deleted E273 and G274 compared to FPbase ID: PGG5S

Promoter

CAG

Available since

Nov. 29, 2022

Availability

Academic Institutions and Nonprofits only

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LV-PGK-Cre

Plasmid

#172433

Purpose

Lentivirus, expresses Cre recombinase

Depositor

Tyler Jacks

Publication

Freed-Pastor et al Cancer Cell. 2021 Oct 11;39(10):1342-136

Insert

Cre

Use

Lentiviral

Available since

June 30, 2023

Availability

Academic Institutions and Nonprofits only

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dimSIIN

Plasmid

#185661

Purpose

shApc-expressing lentiviruses with varius levels of expression of SIINFEKL

Depositor

Tyler Jacks

Publication

Westcott et al Nat Cancer. 2021 Oct;2(10):1071-1085. do

Inserts

mScarlet
miR-30a
shApc
GFP

Use

Lentiviral

Promoter

Ef1a

Available since

Nov. 4, 2022

Availability

Academic Institutions and Nonprofits only

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hiSIIN

Plasmid

#185662

Purpose

shApc-expressing lentiviruses with varius levels of expression of SIINFEKL

Depositor

Tyler Jacks

Publication

Westcott et al Nat Cancer. 2021 Oct;2(10):1071-1085. do

Inserts

mScarlet
miR-30a
shApc

Use

Lentiviral

Promoter

Ef1a

Available since

Nov. 4, 2022

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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U6-sgH11-eCas9-T2A-BlastR

Plasmid

#172492

Purpose

For CRISPR-assisted HDR, Hipp11 locus

Depositor

Tyler Jacks

Publication

Freed-Pastor et al Cancer Cell. 2021 Oct 11;39(10):1342-136

Insert

, BlastR and sgRNA targeting the Hipp11 (H11) safe harbor locus

Use

CRISPR, Lentiviral, and Mouse Targeting

Available since

June 30, 2023

Availability

Academic Institutions and Nonprofits only

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pAAV-syn-FLEX-jYCaMP1s

Plasmid

#135420

Purpose

Yellow protein calcium sensor expressed under human Synapsin1 promoter, Cre mediated flip-excision switch, for AAV production or direct transfection, slow variant

Depositor

Kaspar Podgorski

Publication

Mohr et al Nat Methods. 2020 May 25. pii: 10.1038/s

Has service

AAV1

Insert

jYCaMP1s

Use

AAV

Expression

Mammalian

Promoter

hSyn

Available since

Jan. 7, 2020

Availability

Academic Institutions and Nonprofits only

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loSIIN

Plasmid

#185663

Purpose

shApc-expressing lentiviruses with varius levels of expression of SIINFEKL

Depositor

Tyler Jacks

Publication

Westcott et al Nat Cancer. 2021 Oct;2(10):1071-1085. do

Inserts

mScarlet
miR-30a
shApc
GFP

Use

Lentiviral

Promoter

Ef1a

Available since

Nov. 4, 2022

Availability

Academic Institutions and Nonprofits only

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midSIIN

Plasmid

#185665

Purpose

shApc-expressing lentiviruses with varius levels of expression of SIINFEKL

Depositor

Tyler Jacks

Publication

Westcott et al Nat Cancer. 2021 Oct;2(10):1071-1085. do

Inserts

mScarlet
miR-30a
shApc

Use

Lentiviral

Promoter

Ef1a

Available since

Nov. 4, 2022

Availability

Academic Institutions and Nonprofits only

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LV-PGK-Cre-EFS-mScarletSIIN

Plasmid

#172434

Purpose

Lentivirus, expresses Cre recombinase and mScarlet-SIINFEKL

Depositor

Tyler Jacks

Publication

Freed-Pastor et al Cancer Cell. 2021 Oct 11;39(10):1342-136

Inserts

Cre
mScarlet-SIINFEKL(OVA257-264)+Ova 323-339

Use

Lentiviral

Available since

June 30, 2023

Availability

Academic Institutions and Nonprofits only

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H11-mScarletSIIN

Plasmid

#172438

Purpose

Hipp11 targeting vector, expresses mScarlet-SIINFEKL

Depositor

Tyler Jacks

Publication

Freed-Pastor et al Cancer Cell. 2021 Oct 11;39(10):1342-136

Insert

mScarlet-SIIN

Use

Mouse Targeting

Available since

June 30, 2023

Availability

Academic Institutions and Nonprofits only

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pGAL4DBD-SuUR f.l

Plasmid

#112995

Purpose

P element vector for transposon insertion of full-length Drosophila SuUR into Drosophila genome

Depositor

Terry Orr-Weaver

Publication

Sher et al Genome Res. 2012 Jan;22(1):64-75. doi: 1

Insert

SuUR (SuUR Fly)

Use

P insertion vector

Available since

Aug. 2, 2018

Availability

Academic Institutions and Nonprofits only

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noSIIN

Plasmid

#185666

Purpose

shApc-expressing lentiviruses with no expression of SIINFEKL

Depositor

Tyler Jacks

Publication

Westcott et al Nat Cancer. 2021 Oct;2(10):1071-1085. do

Inserts

mScarlet
miR-30a
shApc

Use

Lentiviral

Promoter

Ef1a

Available since

Nov. 4, 2022

Availability

Academic Institutions and Nonprofits only

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U6-sgfiller-eCas9-T2A-BlastR

Plasmid

#172437

Purpose

Expresses eCas9, BlastR and sgRNA cloning site

Depositor

Tyler Jacks

Publication

Freed-Pastor et al Cancer Cell. 2021 Oct 11;39(10):1342-136

Inserts

eCas9
BlastR

Use

CRISPR, Lentiviral, and Mouse Targeting

Available since

June 30, 2023

Availability

Academic Institutions and Nonprofits only

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H11-mScarletALG8

Plasmid

#172440

Purpose

Hipp11 targeting vector, expresses mScarlet-mALG8

Depositor

Tyler Jacks

Publication

Freed-Pastor et al Cancer Cell. 2021 Oct 11;39(10):1342-136

Insert

mScarlet-mALG8

Use

Mouse Targeting

Available since

June 30, 2023

Availability

Academic Institutions and Nonprofits only

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R26-dCas9-VPR-mNG

Plasmid

#172441

Purpose

Rosa26 targeting vector, expresses dCas9-VPR and mNeonGreen

Depositor

Tyler Jacks

Publication

Freed-Pastor et al Cancer Cell. 2021 Oct 11;39(10):1342-136

Inserts

dCas9-VPR
mNeonGreen

Use

Mouse Targeting

Available since

June 30, 2023

Availability

Academic Institutions and Nonprofits only

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U6-sgR26-eCas9-T2A-BlastR

Plasmid

#172493

Purpose

For CRISPR-assisted HDR, Rosa26 locus

Depositor

Tyler Jacks

Publication

Freed-Pastor et al Cancer Cell. 2021 Oct 11;39(10):1342-136

Insert

eCas9, BlastR and sgRNA targeting the Rosa26 (R26) safe harbor locus

Use

CRISPR, Lentiviral, and Mouse Targeting

Available since

June 30, 2023

Availability

Academic Institutions and Nonprofits only

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CYLBL_Neo_CAGdCas9-KRAB

Plasmid

#220439

Purpose

CAG-dCas9-KRAB knock-in using TALEN at CLYBL locus

Depositor

Yin Shen

Publication

Yang et al Nat Genet. 2023 Oct;55(10):1735-1744. do

Insert

dCas9

Use

CRISPR and TALEN

Tags

KRAB

Expression

Mammalian

Mutation

D10A and H840A

Available since

June 12, 2024

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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LV-U6-sgRNAfiller-PGK-Cre-EFS-mScarletSIIN

Plasmid

#172436

Purpose

Lentivirus, expresses Cre recombinase and mScarlet-SIINFEKL, with sgRNA cloning site

Depositor

Tyler Jacks

Publication

Freed-Pastor et al Cancer Cell. 2021 Oct 11;39(10):1342-136

Inserts

Cre
mScarlet-SIINFEKL(OVA257-264)+Ova 323-339

Use

CRISPR and Lentiviral

Available since

June 30, 2023

Availability

Academic Institutions and Nonprofits only

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