We narrowed to 11,551 results for: NSI

1. pHH0103_SNX18-1/1

   Plasmid

   #91369

   Purpose

   Protein expression and purification of human SH3 domain construct SNX18-1/1

   Depositor

   Sachdev Sidhu

   Article

   Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

   Insert

   SNX18-1/1 (SNX18 Human)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   March 19, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pAB078d9

   Plasmid

   #79207

   Purpose

   Directed Evolution of Protein-Protein Interactions

   Depositor

   David Liu

   Article

   Badran et al Nature. 2016 May 5;533(7601):58-63. doi:

   Insert

   PlacZ-opt (OR1+2+3) luxAB; Ppro1 434cI-SH2ABL1
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   PlacZ-opt (OR1+2+3); Ppro1

   Available Since

   Aug. 21, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pFUGW-ZipACR-EYFP

   Plasmid

   #88844

   Purpose

   Fast anion channelrhodopsin (current half-decay time 2-4 ms depending on voltage) for time-resolved inhibition of neuronal spiking

   Depositor

   John Spudich

   Article

   Govorunova et al Sci Rep. 2017 Mar 3;7:43358. doi: 10.103

   Insert

   ZipACR-NotI-EYFP
   

   Use

   Lentiviral

   Tags

   EYFP

   Expression

   Mutation

   Promoter

   Available Since

   March 21, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. MAC_C_TGON2

   Plasmid

   #111677

   Purpose

   MAC-tagged gene expression

   Depositor

   Markku Varjosalo

   Article

   Liu et al Nat Commun. 2018 Mar 22;9(1):1188. doi:

   Insert

   TGON2 (TGOLN2 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   June 14, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pcDNA_RGG-RFP-RGG

   Plasmid

   #124934

   Purpose

   Expression of RGG-RFP-RGG

   Depositor

   Benjamin Schuster, Matthew Good, Daniel Hammer

   Article

   Schuster et al Nat Commun. 2018 Jul 30;9(1):2985. doi:

   Insert

   RGG-RFP-RGG (using LAF-1 RGG domain) (laf-1 Nematode)
   

   Use

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   May 29, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. IFI16-(192-393)-HinA (pcDNA3-FLAG)

   Plasmid

   #35062

   Depositor

   Cheryl Arrowsmith

   Article

   Liao et al Structure. 2011 Mar 9;19(3):418-29.

   Insert

   IFI16-(192-393)-HinA (IFI16 Human)
   

   Use

   Tags

   FLAG and His

   Expression

   Mammalian

   Mutation

   Contains HinA domain: aa 192-393

   Promoter

   CMV

   Available Since

   April 4, 2012

   Availability

   Academic Institutions and Nonprofits only

7. GFP-CenpB 1-166

   Plasmid

   #108475

   Purpose

   expression of GFP-CenpB 1-166

   Depositor

   Susanne Lens

   Article

   Liu et al Science. 2009 Mar 6;323(5919):1350-3. do

   Insert

   CenpB 1-166 AA (CENPB Human)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   encodes only the DNA-binding domain of CENP-B

   Promoter

   CMV

   Available Since

   May 8, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pHES941

   Plasmid

   #87942

   Purpose

   Constitutively expresses progesterone-inducible synthetic transcription factor in yeast. It activates transcription of promoters containing Gal4 binding sites.

   Depositor

   Hana El-Samad

   Article

   Aranda-Diaz et al ACS Synth Biol. 2016 Dec 27. doi: 10.102

   Insert

   GAL4 DBD - hPR LBD - MSN2 AD (PGR Human, Budding Yeast, Synthetic)
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available Since

   May 25, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. IFI16-(515-710)-HinB (pcDNA3-FLAG)

   Plasmid

   #35063

   Depositor

   Cheryl Arrowsmith

   Article

   Liao et al Structure. 2011 Mar 9;19(3):418-29.

   Insert

   IFI16-(515-710)-HinB (IFI16 Human)
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Contains HinB domain: aa 515-710

   Promoter

   CMV

   Available Since

   April 4, 2012

   Availability

   Academic Institutions and Nonprofits only

10. GW1-mito-mCherryEA

    Plasmid

    #207868

    Purpose

    Mitochondria-targeted mCherry(I158E/Q160A) mutant as a red fluorescent pH sensor

    Depositor

    Mathew Tantama

    Article

    Rajendran et al ACS Omega. 2018 Aug 31;3(8):9476-9486. d

    Insert

    mito-mCherryEA
    

    Use

    Tags

    4xCox8 mitochondrial targeting

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Oct. 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. H2B-mScarlet- hRec8(297-506)-mNeonGreen

    Plasmid

    #174717

    Purpose

    Stable expression of fluorescent Separase cleavage sensor with human Rec8

    Depositor

    Iain Cheeseman

    Article

    Maier et al Dev Cell. 2021 Aug 9;56(15):2192-2206.e8

    Insert

    Rec8 (REC8 Human)
    

    Use

    Retroviral

    Tags

    H2B-mScarlet and mNeonGreen

    Expression

    Mammalian

    Mutation

    297-506

    Promoter

    Available Since

    Oct. 1, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pSLQ8541 PB-PGK-RR12EE345L-C term (406) dLbCpf1-2XNLS-miniVPR-mCherry-pA

    Plasmid

    #140222

    Purpose

    Constitutive PGK driven expression of leucine zipper with C terminal dCpf1 (Cas12a) 406-split half fused to miniVPR

    Depositor

    Stanley Qi

    Article

    Kempton et al Mol Cell. 2020 Jan 30. pii: S1097-2765(2

    Insert

    zipper with dLbCpf1 (Cas12a) C terminal half (406) fused to miniVPR
    

    Use

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    D832A

    Promoter

    PGK

    Available Since

    May 8, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pTorPE-IP2.0

    Plasmid

    #109217

    Purpose

    Express IP2.0 in E. coli

    Depositor

    Takeshi Ishihara

    Article

    Hara-Kuge et al PLoS One. 2018 Apr 25;13(4):e0194707. do

    Insert

    IP2.0 (Inverse Pericam 2.0)
    

    Use

    Tags

    His-tag

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    May 23, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. RIPR-bio-His

    Plasmid

    #50824

    Purpose

    Expresses enzymatically monobiotinylated full length RIPR

    Depositor

    Gavin Wright

    Article

    Zenonos et al Malar J. 2014 Mar 12;13(1):93. doi: 10.1

    Insert

    RIPR
    

    Use

    Tags

    ratCD4d3+4,biotinylation sequence, 6xHis

    Expression

    Mammalian

    Mutation

    all potential N-linked glycosylation sites (N-X-S…

    Promoter

    CMV

    Available Since

    Feb. 11, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. EK0423 SFFV-mTagBFP2-SGc(s70587)cSG-mCherry (FLP-IN)

    Plasmid

    #191162

    Purpose

    Expression of a sensor for synthetic sequence 1 (EK0208) in mammalian cells; mTagBFP2 marker, mCherry output

    Depositor

    Xiaojing Gao

    Article

    Kaseniit et al Nat Biotechnol. 2022 Oct 5. pii: 10.1038

    Insert

    mTagBFP:s70587:mCherry
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    WT

    Promoter

    SFFV

    Available Since

    Oct. 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pHH0103_TNK2-1/1

    Plasmid

    #91377

    Purpose

    Protein expression and purification of human SH3 domain construct TNK2-1/1

    Depositor

    Sachdev Sidhu

    Article

    Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

    Insert

    TNK2-1/1 (TNK2 Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    March 19, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pAB085d

    Plasmid

    #79209

    Purpose

    Directed Evolution of Protein-Protein Interactions

    Depositor

    David Liu

    Article

    Badran et al Nature. 2016 May 5;533(7601):58-63. doi:

    Insert

    PlacZ-opt (OR1) luxAB; Ptet 434cI-TnTBR3-F3
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    PlacZ-opt (OR1); Ptet

    Available Since

    Oct. 3, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. As2

    Plasmid

    #123160

    Purpose

    Arsenic sensor used in the sensor cell array. pSB4A3 carrying J109-30arsR-t-ParsR-ABS84-30hrpR-30hrpS-t-PhrpLE-30gfp-t

    Depositor

    Baojun Wang

    Article

    Wan et al Nat Chem Biol. 2019 May;15(5):540-548. d

    Insert

    J109-30arsR-t-ParsR-ABS84-30hrpR-30hrpS-t-PhrpLE-30gfp-t
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    J109, ParsR-ABS84, PhrpLE

    Available Since

    May 9, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. uORF1-Mreinit- 36xMashTag-BFP-3xStop-24xPP7 (uORF-MashTag reporter)

    Plasmid

    #128609

    Purpose

    Expresses uORF1 -36xMashTag with MoonTag as readout for reinitiation, followed by BFP linker and 24 repeats of PP7 hairpins in 3’ UTR.

    Depositor

    Marvin Tanenbaum

    Article

    Boersma et al Cell. 2019 Jun 5. pii: S0092-8674(19)304

    Insert

    uORF1-36xMashTag
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Pcmv

    Available Since

    Nov. 11, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. JW617

    Plasmid

    #64809

    Purpose

    CUC3 expression in yeast

    Depositor

    Detlef Weigel

    Article

    Rubio-Somoza et al Curr Biol. 2014 Nov 17;24(22):2714-9. do

    Insert

    CUC3 (CUC3 Mustard Weed)
    

    Use

    Tags

    GAL4 DNA binding domain and Myc

    Expression

    Mutation

    Promoter

    Available Since

    Aug. 26, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pXWJ109AsAmp30E

    Plasmid

    #123144

    Purpose

    Arsenic sensor with an optimized HrpRS-based amplifier. pSB3K3 carrying J109-30arsR-t-ParsR-30hrpR-30hrpS-t-PhrpLE-30gfp-t

    Depositor

    Baojun Wang

    Article

    Wan et al Nat Chem Biol. 2019 May;15(5):540-548. d

    Insert

    J109-30arsR-t-ParsR-30hrpR-30hrpS-t-PhrpLE-30gfp-t
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    J109, ParsR, PhrpLE

    Available Since

    May 9, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pcDNA3.1(+)-sapphireAKAR-NLS

    Plasmid

    #118467

    Purpose

    cp-T-sapphire-based single-color PKA biosensor targeted to the nucleus.

    Depositor

    Jin Zhang

    Article

    Mehta et al Nat Cell Biol. 2018 Sep 24. pii: 10.1038

    Insert

    sapphireAKAR-NLS
    

    Use

    Tags

    Nuclear localization signal (NLS)

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Dec. 21, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. N1-mSin1.5-GFP

    Plasmid

    #72909

    Purpose

    GFP-tagged mSin1 (isoform 5)

    Depositor

    Ivan Yudushkin

    Article

    Ebner et al J Cell Biol. 2017 Jan 31. pii: jcb.20161

    Insert

    Target of rapamycin complex 2 subunit MAPKAP1, isoform 5 (MAPKAP1 Human)
    

    Use

    Tags

    eGFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    March 21, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pRK5-mFzd8-1D4

    Plasmid

    #42270

    Depositor

    Chris Garcia, Jeremy Nathans

    Article

    Yu et al Development. 2012 Dec 1;139(23):4383-94.

    Insert

    Frizzled 8 (Fzd8 Mouse)
    

    Use

    Tags

    1D4

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    March 28, 2013

    Availability

    Academic Institutions and Nonprofits only

25. JW624

    Plasmid

    #64808

    Purpose

    CUC2 expression in yeast

    Depositor

    Detlef Weigel

    Article

    Rubio-Somoza et al Curr Biol. 2014 Nov 17;24(22):2714-9. do

    Insert

    CUC2 (CUC2 Mustard Weed)
    

    Use

    Tags

    GAL4 DNA binding domain

    Expression

    Mutation

    Promoter

    Available Since

    Sept. 30, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pCSDest-MYC-TMPRSS2

    Plasmid

    #154964

    Purpose

    TMPRSS2 with N-terminal MYC

    Depositor

    Utpal Pajvani

    Article

    Bartolome et al Sci Rep. 2021 May 7;11(1):9803. doi: 10.

    Insert

    MYC-TMPRSS2 (TMPRSS2 Human)
    

    Use

    Tags

    Myc

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    July 14, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

27. EGFP-UTRN-mini

    Plasmid

    #158749

    Purpose

    Expresses a shorter version of Utrophin (amino acid 35-136) with amino terminus eGFP in mammalian cells

    Depositor

    Minhajuddin Sirajuddin

    Article

    Kumari et al EMBO J. 2020 Jun 22:e104006. doi: 10.152

    Insert

    UTRN-mini
    

    Use

    Tags

    eGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV IE94

    Available Since

    Sept. 24, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. p168

    Plasmid

    #62618

    Purpose

    ePathBrick pETM6 vector encoding CsF3H, FaDFR, and DuLAR

    Depositor

    Mattheos Koffas

    Article

    Zhao et al Metab Eng. 2014 Dec 17;28C:43-53. doi: 1

    Inserts

    flavaone 3B-hydroxylase
    dihydroflavonol 4-reductase
    leucoanthocyanidin reductase
    

    Use

    Synthetic Biology

    Tags

    GBD, PDZ, and SH3

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    July 9, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pACE-GIRK4-S

    Plasmid

    #172425

    Purpose

    Expression of full-length, human GIRK4 with C-terminal StrepTag II

    Depositor

    Arthur Laganowsky

    Article

    Qiao et al Biochemistry. 2021 Dec 21;60(50):3813-38

    Insert

    GIRK4 (KCNJ5 Human)
    

    Use

    Tags

    Streptag II

    Expression

    Insect

    Mutation

    Promoter

    Available Since

    Oct. 12, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pARF1-YFP

    Plasmid

    #11390

    Depositor

    Joel Swanson

    Article

    Beemiller et al PLoS Biol. 2006 May 9. 4(6):e162.

    Insert

    ADP Ribosylation Factor 1 (ARF1 Human)
    

    Use

    Tags

    YFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    May 30, 2006

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pAB088i

    Plasmid

    #79216

    Purpose

    Directed Evolution of Protein-Protein Interactions

    Depositor

    David Liu

    Article

    Badran et al Nature. 2016 May 5;533(7601):58-63. doi:

    Insert

    PlacZ-opt (OR1) gIII, luxAB; Ppro1 434cI(RR69)-TnCAD-F3
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    PlacZ-opt (OR1); Ppro1

    Available Since

    Nov. 8, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. HM3C NaVAe1_p pET24b

    Plasmid

    #127854

    Purpose

    Bacterial expression vector for NaVAe1 bacterial sodium channel residues 143-288. It expresses HIStag-MBP-3C-channel protein.

    Depositor

    Dan Minor

    Article

    Arrigoni et al Cell. 2016 Feb 25;164(5):922-36. doi: 10

    Insert

    NaVAe1p
    

    Use

    Tags

    3C Cleavage site, His, and MBP

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    May 15, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pAB085c

    Plasmid

    #79208

    Purpose

    Directed Evolution of Protein-Protein Interactions

    Depositor

    David Liu

    Article

    Badran et al Nature. 2016 May 5;533(7601):58-63. doi:

    Insert

    PlacZ-opt (OR1) luxAB; Ptet 434cI-TnTBR3-FL
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    PlacZ-opt (OR1); Ptet

    Available Since

    Nov. 8, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. EK0325 SFFV-mCherry-SG(EGFP.s2)SG-Cre (FLP-IN)

    Plasmid

    #191160

    Purpose

    Expression of a sensor for EGFP CDS in mammalian cells; mCherry marker, Cre output

    Depositor

    Xiaojing Gao

    Article

    Kaseniit et al Nat Biotechnol. 2022 Oct 5. pii: 10.1038

    Insert

    mCherry:EGFP.s2:Cre
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    WT

    Promoter

    SFFV

    Available Since

    Oct. 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pET28a_Pro-IL-36β

    Plasmid

    #214344

    Purpose

    Protein overexpression in bacteria

    Depositor

    Jonathan Kagan

    Article

    Devant et al Nature. 2023 Dec;624(7991):451-459. doi:

    Insert

    IL36B (IL36B Human)
    

    Use

    Tags

    His-TEV and Myc

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    Feb. 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pXWJ109HgAmp30E

    Plasmid

    #123149

    Purpose

    Mercury sensor with an optimized HrpRS-based amplifier. pSB3K3 carrying J109-32merR-t-PmerT-30hrpR-30hrpS-t-PhrpLE-30gfp-t

    Depositor

    Baojun Wang

    Article

    Wan et al Nat Chem Biol. 2019 May;15(5):540-548. d

    Insert

    J109-32merR-t-PmerT-30hrpR-30hrpS-t-PhrpLE-30gfp-t
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    J109, PmerT, PhrpLE

    Available Since

    May 9, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pcDNA3.1/Hygro(-)-B2-cT

    Plasmid

    #104355

    Purpose

    Expresses human beta 2 integrin tension sensor

    Depositor

    Pontus Nordenfelt, Timothy Springer

    Article

    Nordenfelt et al Nat Commun. 2016 Oct 10;7:13119. doi: 10

    Insert

    Human integrin b2 with tension sensor (ITGB2 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Dec. 26, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pcDNA3 RL3Sc

    Plasmid

    #109365

    Purpose

    Human rod opsin chimera with intracellular loop 3 from Scallop opsin with 1D4 tag

    Depositor

    Robert Lucas

    Article

    Ballister et al BMC Biol. 2018 Jan 16;16(1):10. doi: 10.

    Insert

    Rod opsin chimera with Scallop Opsin2 Loop 3 (RHO, LOC110451845 Patinopecten yessoensis)
    

    Use

    Tags

    1D4

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    May 9, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pcDNA3.1(+) neo with Ywhag CDS

    Plasmid

    #61799

    Purpose

    pcDNA3.1(+) with full length mouse Ywhag CDS

    Depositor

    Judy Lieberman

    Article

    Perdigao-Henriques et al Oncogene. 2015 Mar 23. doi: 10.1038/onc.

    Insert

    Ywhag CDS (Ywhag Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    May 14, 2015

    Availability

    Academic Institutions and Nonprofits only

40. pRK5-mFzd2-1D4

    Plasmid

    #42264

    Depositor

    Chris Garcia, Jeremy Nathans

    Article

    Yu et al Development. 2012 Dec 1;139(23):4383-94.

    Insert

    Frizzled 2 (Fzd2 Mouse)
    

    Use

    Tags

    1D4

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    March 28, 2013

    Availability

    Academic Institutions and Nonprofits only