We narrowed to 16,109 results for: form

1. LLP734_L2pV1_1I_OCS-rLUC_F-NOS-FlpO

   Plasmid

   #192377

   Purpose

   To test if the NOS promoter can drive the Flp recomhinase in order to remove the OCS terminator and increase circuit output.

   Depositor

   Ryan Lister

   Article

   Lloyd et al Nat Biotechnol. 2022 Jul 4. pii: 10.1038

   Insert

   Act2::FRT-OCS-FRT::Rluc
   

   Use

   Synthetic Biology

   Tags

   PEST

   Expression

   Plant

   Available Since

   Nov. 22, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. LLP735_L2pV1_1I_OCS-rLUC_F-TCTP-FlpO

   Plasmid

   #192378

   Purpose

   To test if the TCTP promoter can drive the Flp recomhinase in order to remove the OCS terminator and increase circuit output.

   Depositor

   Ryan Lister

   Article

   Lloyd et al Nat Biotechnol. 2022 Jul 4. pii: 10.1038

   Insert

   Act2::FRT-OCS-FRT::Rluc
   

   Use

   Synthetic Biology

   Tags

   PEST

   Expression

   Plant

   Available Since

   Nov. 22, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. hsLEMD2-pX330

   Plasmid

   #179511

   Purpose

   Encodes gRNA for human LEMD2

   Depositor

   Yasushi Hiraoka

   Article

   Haraguchi et al Commun Biol. 2022 Jan 20;5(1):78. doi: 1

   Insert

   gRNA for LEMD2
   

   Use

   CRISPR

   Expression

   Mammalian

   Promoter

   U6

   Available Since

   Nov. 2, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. Arabidopsis thaliana Atm3 pET21a+

   Plasmid

   #172321

   Purpose

   Expresses AtAtm3 in E.coli

   Depositor

   Douglas Rees

   Article

   Fan et al Elife. 2022 Mar 25;11. pii: 76140. doi:

   Insert

   Arabidopsis thaliana Atm3 (ABCB25 Mustard Weed)
   

   Tags

   6xHis tag

   Expression

   Bacterial

   Available Since

   Oct. 13, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pCSIIpuro-miRFP670-eDHFR(69K6)

   Plasmid

   #178852

   Purpose

   Mammalian expression of miRFP670-eDHFR(69K6)

   Depositor

   Shinya Tsukiji

   Article

   Suzuki et al Cell Chem Biol. 2022 Sep 15;29(9):1446-1

   Insert

   miRFP670-eDHFR(69K6)
   

   Use

   Lentiviral

   Expression

   Mammalian

   Promoter

   EF1-alpha

   Available Since

   July 14, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pCSIIpuro-mNG-eDHFR(69K6)

   Plasmid

   #178853

   Purpose

   Mammalian expression of mNeonGreen-eDHFR(69K6)

   Depositor

   Shinya Tsukiji

   Article

   Suzuki et al Cell Chem Biol. 2022 Sep 15;29(9):1446-1

   Insert

   mNeonGreen-eDHFR(69K6)
   

   Use

   Lentiviral

   Expression

   Mammalian

   Promoter

   EF1-alpha

   Available Since

   July 14, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pCAGGS-eDHFR(69K6)-iRFP713

   Plasmid

   #178855

   Purpose

   Mammalian expression of eDHFR(69K6)-iRFP713

   Depositor

   Shinya Tsukiji

   Article

   Suzuki et al Cell Chem Biol. 2022 Sep 15;29(9):1446-1

   Insert

   eDHFR(69K6)-iRFP713
   

   Expression

   Mammalian

   Promoter

   CAG

   Available Since

   July 14, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pCMV-miRFP670-eDHFR(69K6)

   Plasmid

   #178859

   Purpose

   Mammalian expression of miRFP670-eDHFR(69K6)

   Depositor

   Shinya Tsukiji

   Article

   Suzuki et al Cell Chem Biol. 2022 Sep 15;29(9):1446-1

   Insert

   miRFP670-eDHFR(69K6)
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   July 14, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. FAK Fus

   Plasmid

   #186145

   Purpose

   For expression of recombinant FAK using baculovirus in SF9 cells

   Depositor

   Michael Rosen

   Article

   Case et al Elife. 2022 Jan 20;11. pii: 72588. doi:

   Insert

   PTK2 (PTK2 Mouse)
   

   Tags

   His6

   Expression

   Insect

   Mutation

   C-terminal linker swapped with FUS IDR

   Promoter

   polyhedrin promoter

   Available Since

   July 11, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pFastBac FAK W266A

    Plasmid

    #186144

    Purpose

    For expresseion of recombinant FAK using baccolovirus in SF9 cells

    Depositor

    Michael Rosen

    Article

    Case et al Elife. 2022 Jan 20;11. pii: 72588. doi:

    Insert

    PTK2 (PTK2 Mouse)
    

    Tags

    His6

    Expression

    Insect

    Mutation

    DNA mutation T796G;G797C Amino acid mutation W266A

    Promoter

    polyhedrin promoter

    Available Since

    June 30, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. Lck

    Plasmid

    #186130

    Purpose

    For expression of recombinant Lck in SF9 cells.

    Depositor

    Michael Rosen

    Article

    Case et al Elife. 2022 Jan 20;11. pii: 72588. doi:

    Insert

    Lck (Lck Mouse)
    

    Tags

    His6

    Expression

    Insect

    Mutation

    A1687T

    Promoter

    polyhedrin promoter

    Available Since

    June 30, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. FAK Pyk2

    Plasmid

    #186146

    Purpose

    For expresseion of recombinant FAK using baccolovirus in SF9 cells

    Depositor

    Michael Rosen

    Article

    Case et al Elife. 2022 Jan 20;11. pii: 72588. doi:

    Insert

    PTK2 (PTK2 Mouse)
    

    Tags

    His6

    Expression

    Insect

    Mutation

    C-terminal linker swapped with Pyk2 linker

    Promoter

    polyhedrin promoter

    Available Since

    June 30, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pLib6.4-Cquin_728

    Plasmid

    #176656

    Purpose

    Expression of sgRNA under C. quinquefasciatus U6 (CPIJ039728) promoter & delivery of D.mel-Actin5C::EGFP-2A-puro|sgRNA cassette through PhiC31 RCME to AttP acceptor cell line/organism

    Depositor

    Norbert Perrimon

    Article

    Viswanatha et al Nat Commun. 2021 Nov 24;12(1):6825. doi:

    Type

    Empty backbone

    Use

    Crispr

    Expression

    Insect

    Available Since

    Nov. 24, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pLib6.4B-Agam_557

    Plasmid

    #176667

    Purpose

    Expression of sgRNA under An. gambiae U6-1 (AGAP013557) promoter & delivery of D.mel-Actin5C::EBFP2-2A-puro|sgRNA cassette through PhiC31 RCME to AttP acceptor cell line/organism

    Depositor

    Norbert Perrimon

    Article

    Viswanatha et al Nat Commun. 2021 Nov 24;12(1):6825. doi:

    Type

    Empty backbone

    Use

    Crispr

    Expression

    Insect

    Available Since

    Nov. 23, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pLib6.4B-Aaeg_972

    Plasmid

    #176674

    Purpose

    Expression of sgRNA under Ae. Aegypti U6 (AAEL028972) promoter & delivery of D.mel-Actin5C::EBFP2-2A-puro|sgRNA cassette through PhiC31 RCME to AttP acceptor cell line/organism

    Depositor

    Norbert Perrimon

    Article

    Viswanatha et al Nat Commun. 2021 Nov 24;12(1):6825. doi:

    Type

    Empty backbone

    Use

    Crispr

    Expression

    Insect

    Available Since

    Nov. 23, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pLib6.4-Agam_557

    Plasmid

    #176653

    Purpose

    Expression of sgRNA under An. gambiae U6-1 (AGAP013557) promoter & delivery of D.mel-Actin5C::EGFP-2A-puro|sgRNA cassette through PhiC31 RCME to AttP acceptor cell line/organism

    Depositor

    Norbert Perrimon

    Article

    Viswanatha et al Nat Commun. 2021 Nov 24;12(1):6825. doi:

    Type

    Empty backbone

    Use

    Crispr

    Expression

    Insect

    Available Since

    Nov. 23, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pLib6.4B-Aalb_726

    Plasmid

    #176675

    Purpose

    Expression of sgRNA under Ae. albopictus U6 (AALF029726) promoter & delivery of D.mel-Actin5C::EBFP2-2A-puro|sgRNA cassette through PhiC31 RCME to AttP acceptor cell line/organism

    Depositor

    Norbert Perrimon

    Article

    Viswanatha et al Nat Commun. 2021 Nov 24;12(1):6825. doi:

    Type

    Empty backbone

    Use

    Crispr

    Expression

    Insect

    Available Since

    Nov. 23, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pLib6.4B-Aaeg_763

    Plasmid

    #176672

    Purpose

    Expression of sgRNA under Ae. Aegypti U6 (AAEL017763) promoter & delivery of D.mel-Actin5C::EBFP2-2A-puro|sgRNA cassette through PhiC31 RCME to AttP acceptor cell line/organism

    Depositor

    Norbert Perrimon

    Article

    Viswanatha et al Nat Commun. 2021 Nov 24;12(1):6825. doi:

    Type

    Empty backbone

    Use

    Crispr

    Expression

    Insect

    Available Since

    Nov. 23, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pLib6.4-Aaeg_972

    Plasmid

    #176660

    Purpose

    Expression of sgRNA under Ae. Aegypti U6 (AAEL028972) promoter & delivery of D.mel-Actin5C::EGFP-2A-puro|sgRNA cassette through PhiC31 RCME to AttP acceptor cell line/organism

    Depositor

    Norbert Perrimon

    Article

    Viswanatha et al Nat Commun. 2021 Nov 24;12(1):6825. doi:

    Type

    Empty backbone

    Use

    Crispr

    Expression

    Insect

    Available Since

    Nov. 23, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pLib6.4-Cquin_801

    Plasmid

    #176657

    Purpose

    Expression of sgRNA under C. quinquefasciatus U6 (CPIJ039801) promoter & delivery of D.mel-Actin5C::EGFP-2A-puro|sgRNA cassette through PhiC31 RCME to AttP acceptor cell line/organism

    Depositor

    Norbert Perrimon

    Article

    Viswanatha et al Nat Commun. 2021 Nov 24;12(1):6825. doi:

    Type

    Empty backbone

    Use

    Crispr

    Expression

    Insect

    Available Since

    Nov. 23, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pLib6.4B-Cquin_801

    Plasmid

    #176671

    Purpose

    Expression of sgRNA under C. quinquefasciatus U6 (CPIJ039801) promoter & delivery of D.mel-Actin5C::EBFP2-2A-puro|sgRNA cassette through PhiC31 RCME to AttP acceptor cell line/organism

    Depositor

    Norbert Perrimon

    Article

    Viswanatha et al Nat Commun. 2021 Nov 24;12(1):6825. doi:

    Type

    Empty backbone

    Use

    Crispr

    Expression

    Insect

    Available Since

    Nov. 23, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pLib6.4B-Cquin_728

    Plasmid

    #176670

    Purpose

    Expression of sgRNA under C. quinquefasciatus U6 (CPIJ039728) promoter & delivery of D.mel-Actin5C::EBFP2-2A-puro|sgRNA cassette through PhiC31 RCME to AttP acceptor cell line/organism

    Depositor

    Norbert Perrimon

    Article

    Viswanatha et al Nat Commun. 2021 Nov 24;12(1):6825. doi:

    Type

    Empty backbone

    Use

    Crispr

    Expression

    Insect

    Available Since

    Nov. 23, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pET29b-AviTag(K10A)-eGFP-dspB(E184Q)-6xHis

    Plasmid

    #175788

    Purpose

    Plasmid for overexpression of recombinant AviTagged(nonfunctional), His-tagged fusion protein eGFP-DspB(E184Q), used as a fluorescent probe for detection of biofilm polysaccharide PNAG.

    Depositor

    Mark Nitz

    Article

    Eddenden et al ACS Chem Biol. 2020 May 15;15(5):1204-12

    Insert

    Dispersin B
    

    Tags

    AviTag, Hexahistidine tag, and eGFP

    Expression

    Bacterial

    Mutation

    E184Q

    Promoter

    T7

    Available Since

    Oct. 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pDONR221 CARD8 ZUC

    Plasmid

    #169964

    Purpose

    pDONR221 for Gateway shuttling. Contains the CARD8 FIIND and CARD (L162M-L537, no stop codon)

    Depositor

    Daniel Bachovchin

    Article

    Sharif et al Immunity. 2021 Jul 13;54(7):1392-1404.e1

    Insert

    CARD8 (CARD8 Human)
    

    Use

    Gateway cloning vector

    Available Since

    Sept. 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. 4LNDD-pCDNA3.1(+)

    Plasmid

    #172227

    Purpose

    Expresses human ADAR2 Deaminase Domain (DD) fused with 4 repeats of lambda N peptide (4LN) in mammalian cells

    Depositor

    Joshua Rosenthal

    Article

    Montiel-Gonzalez et al Nucleic Acids Res. 2016 Dec 1;44(21):e15

    Insert

    Lambda N peptide - human ADAR2 Deaminase Domain
    

    Tags

    6xHis and Flag

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Aug. 11, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pCAG-ChroMD-mRuby2-ST

    Plasmid

    #171158

    Purpose

    Cation channelrhodopsin ChroMD fused to mRuby2 fluorophore and targeted to the neuronal soma and proximal dendrites

    Depositor

    Hillel Adesnik

    Article

    Sridharan et al Neuron. 2022 Jan 27. pii: S0896-6273(22)

    Insert

    ChroMD
    

    Expression

    Mammalian

    Available Since

    Aug. 2, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pLyGo-Bs-4

    Plasmid

    #163142

    Purpose

    pLyGo cloning vector for a sequence of interest (LPMO) in B. subtilis. Vector encoding the BatLPMO10 signal peptide and the LyGo cassette (SapI-ccdB-SapI)

    Depositor

    Morten Norholm

    Article

    Hernandez-Rollan et al ACS Synth Biol. 2021 Apr 16;10(4):897-90

    Type

    Empty backbone

    Use

    Synthetic Biology

    Expression

    Bacterial

    Promoter

    PamyL-PamyQ-PcryIIIA-cryIIIAstab49

    Available Since

    July 30, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pLyGo-Ec-4

    Plasmid

    #163130

    Purpose

    pLyGo cloning vector for periplasmic expression in E. coli of a sequence of interest (LPMO). Vector encoding PhoA signal peptide and the LyGo cassette (SapI-ccdB-SapI)

    Depositor

    Morten Norholm

    Article

    Hernandez-Rollan et al ACS Synth Biol. 2021 Apr 16;10(4):897-90

    Type

    Empty backbone

    Use

    Synthetic Biology

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    July 30, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pmx-puro-Mef2c-i

    Plasmid

    #172398

    Purpose

    Retroviral expression vector for murine direct cardiac reprogramming

    Depositor

    Li Qian

    Article

    Wang et al Cells. 2020 Jan 22;9(2). pii: cells90202

    Insert

    Mef2c-i (Mef2c Mouse)
    

    Use

    Retroviral

    Available Since

    July 28, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. Ash1

    Plasmid

    #169711

    Purpose

    Bacterial Expression of yeast Ash1 truncation (encodes amino acids 420-500)

    Depositor

    Tanja Mittag

    Article

    Martin et al J Am Chem Soc. 2016 Nov 30;138(47):15323

    Insert

    Ash1 (ASH1 Budding Yeast)
    

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    Truncation including encoding amino acids 420-500

    Promoter

    T7

    Available Since

    June 11, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. MSCVpic2hygro-EGFP-FF2

    Plasmid

    #164921

    Purpose

    Expresses EGFP cDNA and FF2 shRNA in mammalian cells

    Depositor

    Ji Luo

    Article

    Smith et al Cell Biosci. 2016 Nov 11;6:57. doi: 10.1

    Inserts

    Enhanced green fluorescent protein
    Neuropeptide FF receptor 2 (NPFFR2 Human)
    

    Use

    Retroviral

    Available Since

    May 26, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pDONR221 CARD8 FIIND

    Plasmid

    #169963

    Purpose

    pDONR221 for Gateway shuttling. Contains the FIIND (L162M-P434, no stop codon)

    Depositor

    Daniel Bachovchin

    Article

    Sharif et al Immunity. 2021 Jul 13;54(7):1392-1404.e1

    Insert

    CARD8 (CARD8 Human)
    

    Use

    Gateway cloning vector

    Available Since

    May 12, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pLyGo-Bs-3

    Plasmid

    #163141

    Purpose

    pLyGo cloning vector for a sequence of interest (LPMO) in B. subtilis. Vector encoding the AprE signal peptide and the LyGo cassette (SapI-ccdB-SapI)

    Depositor

    Morten Norholm

    Article

    Hernandez-Rollan et al ACS Synth Biol. 2021 Apr 16;10(4):897-90

    Type

    Empty backbone

    Use

    Synthetic Biology

    Expression

    Bacterial

    Promoter

    PamyL-PamyQ-PcryIIIA-cryIIIAstab49

    Available Since

    May 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pLyGo-Bs-2

    Plasmid

    #163140

    Purpose

    pLyGo cloning vector for a sequence of interest (LPMO) in Bacillus subtilis. Vector encoding the AmyQ signal peptide and the LyGo cassette (SapI-ccdB-SapI)

    Depositor

    Morten Norholm

    Article

    Hernandez-Rollan et al ACS Synth Biol. 2021 Apr 16;10(4):897-90

    Type

    Empty backbone

    Use

    Synthetic Biology

    Expression

    Bacterial

    Promoter

    PamyL-PamyQ-PcryIIIA-cryIIIAstab49

    Available Since

    May 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pLyGo-Ec-3

    Plasmid

    #163129

    Purpose

    pLyGo cloning vector for periplasmic expression in E. coli of a sequence of interest (LPMO). Vector encoding OmpA signal peptide and the LyGo cassette (SapI-ccdB-SapI)

    Depositor

    Morten Norholm

    Article

    Hernandez-Rollan et al ACS Synth Biol. 2021 Apr 16;10(4):897-90

    Type

    Empty backbone

    Use

    Synthetic Biology

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    May 6, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pLyGo-Ec-5

    Plasmid

    #163131

    Purpose

    pLyGo cloning vector for periplasmic expression in E. coli of a sequence of interest (LPMO). Vector encoding DsBA signal peptide and the LyGo cassette (SapI-ccdB-SapI)

    Depositor

    Morten Norholm

    Article

    Hernandez-Rollan et al ACS Synth Biol. 2021 Apr 16;10(4):897-90

    Type

    Empty backbone

    Use

    Synthetic Biology

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    April 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. psiCHECK2 3'UTR GAC

    Plasmid

    #110401

    Purpose

    Luminescence-based glutaminase GAC isoform 3'UTR-mediated RNA stability reporter

    Depositor

    Sandra Martha Gomes Dias

    Insert

    GLS glutaminase (GLS Human)
    

    Use

    Luciferase

    Expression

    Mammalian

    Promoter

    SV40

    Available Since

    Feb. 23, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pDB.His.MBP.3C CARD8 UPA-STOP

    Plasmid

    #164031

    Purpose

    Bacterial expression vector encoding a HRV 3C-cleavable His-MBP-tagged CARD8 UPA

    Depositor

    Hao Wu

    Article

    Robert Hollingsworth et al Nat Commun. 2021 Jan 8;12(1):189. doi: 1

    Insert

    CARD8 (CARD8 Human)
    

    Tags

    His6-MBP-3C

    Expression

    Bacterial

    Available Since

    Feb. 19, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. Ad-HisFlag-USP19ER

    Plasmid

    #155244

    Purpose

    for generating adenoviruses expressing the ER isoform of rat USP19

    Depositor

    Simon Wing

    Article

    Wiles et al Mol Biol Cell. 2015 Mar 1;26(5):913-23.

    Insert

    USP19 (ER isoform) (Usp19 Rat)
    

    Use

    Adenoviral

    Tags

    6XHis-3XFlag

    Mutation

    H118R- please see dep. comments

    Promoter

    CMV

    Available Since

    Jan. 13, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pBbdCas9S(-10AC1)_Psyn-sgRNArodA

    Plasmid

    #149659

    Purpose

    all-in-one CRISPRi vector for targeting B. burgdorferi rodA

    Depositor

    Christine Jacobs-Wagner

    Article

    Takacs et al Appl Environ Microbiol. 2020 Nov 30. pii

    Insert

    dCas9, lacI, sgRNArodA
    

    Use

    CRISPR

    Expression

    Bacterial

    Promoter

    PflaB, PpQE30(-10AC1), Psyn

    Available Since

    Nov. 30, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

41. pBbdCas9S(-10AC2)_Psyn-sgRNArodA

    Plasmid

    #149660

    Purpose

    all-in-one CRISPRi vector for targeting B. burgdorferi rodA

    Depositor

    Christine Jacobs-Wagner

    Article

    Takacs et al Appl Environ Microbiol. 2020 Nov 30. pii

    Insert

    dCas9, lacI, sgRNArodA
    

    Use

    CRISPR

    Expression

    Bacterial

    Promoter

    PflaB, PpQE30(-10AC2), Psyn

    Available Since

    Nov. 25, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

42. pBbdCas9S(-10TC)_Psyn-sgRNAmreB

    Plasmid

    #149595

    Purpose

    all-in-one CRISPRi vector for targeting B. burgdorferi mreB

    Depositor

    Christine Jacobs-Wagner

    Article

    Takacs et al Appl Environ Microbiol. 2020 Nov 30. pii

    Insert

    dCas9, lacI, sgRNAmreB
    

    Expression

    Bacterial

    Promoter

    PflaB, PpQE30(-10TC), Psyn

    Available Since

    Nov. 25, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

43. pBbdCas9S(-10TC)_Psyn-sgRNAftsI

    Plasmid

    #149591

    Purpose

    all-in-all CRISPRi vector for targeting B. burgdorferi ftsI

    Depositor

    Christine Jacobs-Wagner

    Article

    Takacs et al Appl Environ Microbiol. 2020 Nov 30. pii

    Insert

    dCas9, lacI, sgRNAftsI
    

    Use

    CRISPR

    Expression

    Bacterial

    Promoter

    PflaB, PpQE30(-10TC), Psyn

    Available Since

    Nov. 24, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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44. pcDNA-HisFlag-rUSP19ER-CAmut

    Plasmid

    #155237

    Purpose

    for mammalian overexpression of the mutated ER isoform of rat USP19

    Depositor

    Simon Wing

    Article

    Lu et al Mol Cell Biol. 2009 Jan;29(2):547-58. do

    Insert

    USP19 (ER isoform) (Usp19 Rat)
    

    Tags

    6XHis-3XFlag

    Expression

    Mammalian

    Mutation

    Cys(545) to Ala

    Promoter

    CMV

    Available Since

    Oct. 30, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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45. pBbdCas9S(-10TC)_Psyn-sgRNArodA

    Plasmid

    #149657

    Purpose

    all-in-one CRISPRi vector for targeting B. burgdorferi rodA

    Depositor

    Christine Jacobs-Wagner

    Article

    Takacs et al Appl Environ Microbiol. 2020 Nov 30. pii

    Insert

    dCas9, lacI, sgRNArodA
    

    Use

    CRISPR

    Expression

    Bacterial

    Promoter

    PflaB, PpQE30(-10TC), Psyn

    Available Since

    Oct. 26, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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46. pBbdCas9S(-10TC)_Psyn-sgRNAflaB

    Plasmid

    #149589

    Purpose

    all-in-one CRISPRi vector for targeting B. burgdorferi flaB

    Depositor

    Christine Jacobs-Wagner

    Article

    Takacs et al Appl Environ Microbiol. 2020 Nov 30. pii

    Insert

    dCas9, lacI, sgRNAflaB
    

    Expression

    Bacterial

    Promoter

    PflaB, PpQE30(-10TC), Psyn

    Available Since

    Oct. 26, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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47. pCASP-SptP35-TD-HilA

    Plasmid

    #153332

    Purpose

    Triggers expression of SptP35 fused to the HA4-7c12 Tandem Monobody from Salmonella upon induction with arabinose

    Depositor

    Josef Penninger

    Article

    Chabloz et al Commun Biol. 2020 Jul 3;3(1):342. doi: 1

    Insert

    HA4-7c12 Tandem Monobody
    

    Use

    Salmonella expression

    Available Since

    July 21, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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48. pCASP-SptP92-TD-HilA

    Plasmid

    #153331

    Purpose

    Triggers secretion of SptP92 fused to the HA4-7c12 Tandem Monobody from Salmonella upon induction with arabinose

    Depositor

    Josef Penninger

    Article

    Chabloz et al Commun Biol. 2020 Jul 3;3(1):342. doi: 1

    Insert

    SptP92-HA4-7c12 Tandem Monobody
    

    Use

    Salmonella expression

    Available Since

    July 21, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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49. pCASP-3G124-HilA

    Plasmid

    #153324

    Purpose

    Shows that expression of the protein of interest from Salmonella is disrupted when SptP signal is removed from the pCASP-HilA vector

    Depositor

    Josef Penninger

    Article

    Chabloz et al Commun Biol. 2020 Jul 3;3(1):342. doi: 1

    Insert

    3G124 DARPin but no expression
    

    Use

    Salmonella expression

    Available Since

    July 16, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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50. pBz172_CsoS2_N1_GFP

    Plasmid

    #140852

    Purpose

    His-tagged first N-peptide of NTD fused to Superfolder GFP ([N1]-GFP) used for BLI and MST, pET-14b backbone

    Depositor

    David Savage

    Article

    Oltrogge et al Nat Struct Mol Biol. 2020 Mar;27(3):281-

    Insert

    CsoS2 NTD repeat 1 fused to GFP
    

    Tags

    Hexahistidine tag, Strep-Tag II, and Superfolder …

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    June 19, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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