We narrowed to 13,780 results for: TIM

1. pNL(CMV)TK2ALuc2/CMV/WPREdU3

   Plasmid

   #163965

   Purpose

   Co-expression of HSV-1 TK and firefly luciferase in mammalian cells

   Depositor

   Jakob Reiser

   Article

   Argaw et al Mol Ther Methods Clin Dev. 2021 Apr 24;2

   Insert

   HSV-1 TK and FLuc
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   May 25, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pAC1749

   Plasmid

   #127983

   Purpose

   AMA1 plasmid with Aspergillus optimized Cpf1, hygB selection marker, and USER cassette (PacI/Nt.BbvCI)

   Depositor

   Uffe Mortensen

   Article

   Vanegas et al Fungal Biol Biotechnol. 2019 May 1;6:6.

   Insert

   Cpf1
   

   Use

   CRISPR; Fungal expression

   Tags

   SV40 NLS

   Expression

   Bacterial

   Mutation

   codon optimized for Aspergillus nidulans

   Promoter

   Aspergillus nidulans tef1 promoter

   Available since

   Aug. 19, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. pT3TS_Transposase

   Plasmid

   #109768

   Purpose

   Codon optimised transposase sequence in an optimal vector for in vitro transcription. Contains the T3 promoter and synthetic polyA tract.

   Depositor

   Rob Parton

   Article

   Hall et al Nat Commun. 2020 Jul 24;11(1):3711. doi:

   Insert

   Tol2 Transposase
   

   Use

   Zebrafish plasmids

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Feb. 27, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. TagBFP2-C1-SACM1LdeltaTMD-Fis1tail

   Plasmid

   #220078

   Purpose

   SAC1 with the TMD deleted and replaced with the Fis1tail to replace the TMD and target the mitochondria instead of the ER with a Blue Fluorophore

   Depositor

   Gerry Hammond

   Article

   Doyle et al Contact (Thousand Oaks). 2024 Feb 7;7:25

   Insert

   SACM1l-Fis1tail (SACM1L Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   deltaTMD

   Promoter

   Available since

   May 30, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. EGFP-1-SAC1deltaTMD-Fis1tail

   Plasmid

   #220077

   Purpose

   SAC1 with the TMD deleted and replaced with the Fis1tail to replace the TMD and target the mitochondria instead of the ER with a Green Fluorophore

   Depositor

   Gerry Hammond

   Article

   Doyle et al Contact (Thousand Oaks). 2024 Feb 7;7:25

   Insert

   SACM1l-Fis1tail (SACM1L Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   deltaTMD

   Promoter

   Available since

   May 30, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pUCBB-ctH6-eGFP

   Plasmid

   #32562

   Depositor

   Claudia Schmidt-dannert

   Article

   Vick et al Appl Microbiol Biotechnol. 2011 Dec;92(6

   Insert

   eGFP (eGFP )
   

   Use

   Synthetic Biology

   Tags

   6 His tag with Thrombin site

   Expression

   Mutation

   Promoter

   Consitutive lac

   Available since

   Oct. 18, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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7. pKB083

   Plasmid

   #181952

   Purpose

   IPTG-inducible expression of PhoQ

   Depositor

   Jeffrey Tabor

   Article

   Butcher et al Proc Natl Acad Sci U S A. 2022 Aug 30;11

   Insert

   PhoQ; lacI (lacI PhoQ - Salmonella enterica subsp. enterica serovar Typhimurium; lacI - E. coli)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   PhoQ-Ptac; lacI-PlacIq

   Available since

   Oct. 26, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. p2,0_3E5E_eGFP

   Plasmid

   #69359

   Purpose

   Encodes for Ebola Minigenome with eGFP reporter gene under control of a T7 RNA polymerase promoter

   Depositor

   Elke Mühlberger

   Article

   Schmidt et al J Infect Dis. 2011 Nov;204 Suppl 3:S861-

   Insert

   3E5E eGFP minigenome
   

   Use

   T7

   Tags

   Expression

   Mutation

   Promoter

   T7

   Available since

   Nov. 30, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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9. pEGFP-LacI-NLS-VP16

   Plasmid

   #103836

   Purpose

   Transcription activator (transactivation domain of viral VP16 protein) that is constitutively recruited lacO arrays

   Depositor

   Karsten Rippe

   Article

   Rademacher et al J Cell Sci. 2017 Dec 15;130(24):4213-422

   Insert

   EGFP-LacI-NLS-VP16
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   SV2

   Available since

   Jan. 9, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. pPaxillin-Electra2

    Plasmid

    #184926

    Purpose

    Expresses paxillin fused to blue fluorescent protein Electra2

    Depositor

    Kiryl Piatkevich

    Article

    Papadaki et al Sci Rep. 2022 Jun 17;12(1):10190. doi: 1

    Insert

    paxillin-Electra2
    

    Use

    Tags

    Electra2

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 7, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. pUCmini-UGCG-tdTomato

    Plasmid

    #225879

    Purpose

    Plasmid donor template for CRISPR/Cas9 mediated UGCG knockout by tdTomato insertion

    Depositor

    Yohei Sato

    Article

    Sato et al Biochem Biophys Res Commun. 2024 Sep 18;

    Insert

    tdTomato
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 10, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. pFLEX+mCyclin D1

    Plasmid

    #86793

    Purpose

    mammalian expression of cyclin D1

    Depositor

    Martine Roussel, Charles Sherr

    Article

    Matsushime et al Cell. 1991 May 17;65(4):701-13.

    Insert

    cyclin D1 (Ccnd1 Mouse)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 14, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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13. LAMP-CeNL_pcDNA3

    Plasmid

    #89514

    Purpose

    Cyan color variant of bright luminescent protein, fused to Lysosomal-associated membrane protein 1

    Depositor

    Takeharu Nagai

    Article

    Suzuki et al Nat Commun. 2016 Dec 14;7:13718. doi: 10

    Insert

    CeNL
    

    Use

    Tags

    LAMP1

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 19, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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14. pYZ033

    Plasmid

    #98404

    Purpose

    Entry vector for S. pombe CRISPR-Cas9 system. The gRNA can be integrated easily through Gibson Assembly with the plasmid backbone digested by Not1

    Depositor

    Jef Boeke

    Article

    Zhao et al G3 (Bethesda). 2018 Jan 10. pii: g3.117.

    Type

    Empty backbone

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 5, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. PE_CO

    Plasmid

    #190335

    Purpose

    Prime editor (PE2) with codon-optimized Reverse transcriptase for prime editing in mammalian cells

    Depositor

    Rasmus Bak

    Article

    Gao et al Mol Ther. 2022 Jul 8. pii: S1525-0016(22

    Insert

    PE2 with codon-optimized reverse transcriptase
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. AAV-FLEX-CAG-CyRFP1

    Plasmid

    #84357

    Purpose

    Expresses CyRFP1 fluorescent protein in an AAV backbone, FLEX version (Cre dependent)

    Depositor

    Ryohei Yasuda

    Article

    Laviv et al Nat Methods. 2016 Oct 31. doi: 10.1038/n

    Insert

    CyRFP1
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Double FLEXed

    Promoter

    CAG

    Available since

    Nov. 22, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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17. LT2_14_GlyOx

    Plasmid

    #177711

    Purpose

    Plasmid for genome integration in X2 site expressing GlyRNA and OxPro (biosensors for glycolytic flux and oxidative stress)

    Depositor

    Lisbeth Olsson

    Article

    Torello Pianale et al Front Microbiol. 2022 Jan 7;12:802169. d

    Inserts

    pTEFmut8-mTurquoise2-RNA_2.6--tCYC1
    pTEFmut8-mCherry-tADH1
    pTRX2_5xUAS-ymYPET-tCYC1
    

    Use

    Used as donor dna in genome integration after lin…

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Jan. 13, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. HBT_pcoCASphi_version1

    Plasmid

    #197931

    Purpose

    Cloning vector with Arabidopsis codon optimized Casphi-2 protein, with SV40 NLS at the N-terminal and nucleoplasmin NLS at the C-terminal

    Depositor

    Steven Jacobsen

    Article

    Li et al Proc Natl Acad Sci U S A. 2023 Jan 24;12

    Insert

    pcoCasphi-2
    

    Use

    CRISPR

    Tags

    FLAG tag and SV40 NLS and nucleoplasmin NLS

    Expression

    Plant

    Mutation

    Promoter

    35SPPDK promoter

    Available since

    March 30, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. GFP-C1-CAMKIIalpha-T286A

    Plasmid

    #21220

    Depositor

    Tobias Meyer

    Article

    Shen et al Science. 1999 Apr 2. 284(5411):162-6.

    Insert

    CAMKIIalpha-T286A (Camk2a Rat)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    T286A (Lab plasmid KS 0003).

    Promoter

    Available since

    July 29, 2009

    Availability

    Academic Institutions and Nonprofits only

20. pESC-URA::CYP71AV1/CPR

    Plasmid

    #20118

    Depositor

    Jay Keasling

    Article

    Ro et al Nature. 2006 Apr 13. 440(7086):940-3.

    Insert

    amorphadiene oxidase and p450 reductase
    

    Use

    Synthetic Biology

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    March 2, 2009

    Availability

    Academic Institutions and Nonprofits only