59,474 results

1. PDZK1_PDZ_2

   Plasmid

   #103943

   Purpose

   Protein expression and purification of PDZK1 PDZ2 domain

   Depositor

   Sachdev Sidhu

   Article

   Tonikian et al PLoS Biol. 2008 Sep 30;6(9):e239. doi: 1

   Insert

   PDZK1 (PDZK1 Human)
   

   Tags

   GST

   Expression

   Bacterial

   Promoter

   tac

   Available Since

   Aug. 29, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. PDZK1_PDZ_3

   Plasmid

   #103944

   Purpose

   Protein expression and purification of PDZK1 PDZ3 domain

   Depositor

   Sachdev Sidhu

   Article

   Tonikian et al PLoS Biol. 2008 Sep 30;6(9):e239. doi: 1

   Insert

   PDZK1 (PDZK1 Human)
   

   Tags

   GST

   Expression

   Bacterial

   Promoter

   tac

   Available Since

   Aug. 29, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. pFastBac-ATG101-ATG13 HORMA

   Plasmid

   #99332

   Purpose

   Mammalian expression of one subcomplex from the Human ULK1 complex

   Depositor

   James Hurley

   Article

   Qi et al Structure. 2015 Oct 6;23(10):1848-57. do

   Inserts

   ATG101 (ATG101 Human)
   ATG13 (ATG13 Human)
   

   Tags

   GST

   Expression

   Insect

   Promoter

   p10 and pH

   Available Since

   Aug. 21, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. RFP-LC3B

   Plasmid

   #200943

   Purpose

   RFP fused with LC3B for reporting LC3B lipidation

   Depositor

   Nir Hacohen

   Article

   Liu et al Science. 2023 Aug 4;381(6657):508-514. d

   Insert

   LC3B (MAP1LC3B Human)
   

   Use

   Lentiviral

   Available Since

   Sept. 14, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. VPS13C^mClover3

   Plasmid

   #118760

   Purpose

   VPS13C internally tagged with mClover3 at residue 1914.

   Depositor

   Pietro De Camilli

   Article

   Kumar et al J Cell Biol. 2018 Oct 1;217(10):3625-363

   Insert

   VPS13C (VPS13C Human)
   

   Tags

   mClover3 inserted at position aa 1914

   Expression

   Mammalian

   Available Since

   Nov. 16, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. pEGFP-coilin

   Plasmid

   #36906

   Depositor

   Greg Matera

   Article

   Shpargel et al J Cell Sci. 2003 Jan 15;116(Pt 2):303-12

   Insert

   coilin (COIL Human)
   

   Tags

   EGFP

   Expression

   Mammalian

   Available Since

   Feb. 22, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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7. pFUW-tetO-NFKB1

   Plasmid

   #139822

   Purpose

   doxycycline-inducible expression of Human NFKB1 in mammalian cells

   Depositor

   Filipe Pereira

   Article

   Rosa et al Sci Immunol. 2018 Dec 7;3(30). pii: 3/30

   Insert

   NFKB1 (NFKB1 Human)
   

   Use

   Lentiviral

   Expression

   Mammalian

   Promoter

   TRE-mCMV

   Available Since

   May 21, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. pcDNA3 HA Arf6 ActQ67L

   Plasmid

   #10835

   Depositor

   Thomas Roberts

   Article

   Furman et al J Biol Chem. 2002 Mar 8. 277(10):7962-9.

   Insert

   Arf6 Q67L (ARF6 Human)
   

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Q67L. Defective in GTP hydrolysis. Constitutive…

   Available Since

   April 4, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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9. mCherry-Parkin

   Plasmid

   #23956

   Purpose

   Mammalian expression of human Park2 fused to mCherry

   Depositor

   Richard Youle

   Article

   Narendra et al J Cell Biol. 2008 Dec 1. 183(5):795-803.

   Insert

   Park2 (PRKN Human)
   

   Tags

   mCherry

   Expression

   Mammalian

   Available Since

   March 24, 2010

   Availability

   Academic Institutions and Nonprofits only

   

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10. pEGFP-LC3 (human)

    Plasmid

    #24920

    Purpose

    Mammalian expression of LC3 fused to EGFP

    Depositor

    Toren Finkel

    Article

    Lee et al Proc Natl Acad Sci U S A. 2008 Mar 4. 10

    Insert

    LC3 (MAP1LC3B Human)
    

    Tags

    EGFP

    Expression

    Mammalian

    Available Since

    June 10, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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11. pDONR223_CDKN1A_WT

    Plasmid

    #82201

    Purpose

    Gateway Donor vector containing CDKN1A , part of the Target Accelerator Plasmid Collection.

    Depositor

    Jesse Boehm, William Hahn, David Root

    Article

    Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

    Insert

    CDKN1A (CDKN1A Human)
    

    Use

    Gateway entry vector

    Mutation

    WT

    Promoter

    None

    Available Since

    April 24, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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12. pLenti puro HA-Ubiquitin

    Plasmid

    #74218

    Purpose

    Lentiviral expression of HA tagged Ubiquitin

    Depositor

    Melina Fan

    Insert

    ubiquitin (UBB Human)
    

    Use

    Lentiviral

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Single ubiquitin coding sequence (full length UBB…

    Promoter

    CMV

    Available Since

    March 14, 2016

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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13. pEGFP-Akt1(WT)

    Plasmid

    #86637

    Purpose

    Expresses eGFP-tagged human Akt1(WT)

    Depositor

    Thomas Leonard, Ivan Yudushkin

    Article

    Ebner et al Mol Cell. 2017 Feb 2;65(3):416-431.e6. d

    Insert

    Akt1 (AKT1 Human)
    

    Expression

    Mammalian

    Available Since

    March 21, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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14. pcDNA3-CXCL12-sfGFP

    Plasmid

    #98961

    Purpose

    Mammalian expression plasmid for superfolder GFP-fused human chemokine CXCL12

    Depositor

    Erik Procko

    Article

    Heredia et al J Immunol. 2018 Apr 20. pii: jimmunol.18

    Insert

    CXCL12 (CXCL12 Human)
    

    Tags

    Superfolder GFP

    Expression

    Mammalian

    Mutation

    Full-length, wildtype

    Promoter

    CMV

    Available Since

    April 24, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. FUW-tetO-NFIA

    Plasmid

    #141403

    Purpose

    Inducible Expression of NFIA

    Depositor

    Lorenz Studer

    Article

    Tchieu et al Nat Biotechnol. 2019 Mar;37(3):267-275.

    Insert

    NFIA (NFIA Human)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Available Since

    July 9, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. pAAV-hSyn-GRAB_eCB2.0

    Plasmid

    #164604

    Purpose

    Express the endocannabinoid sensor GRAB_eCB2.0 in neurons

    Depositor

    Yulong Li

    Article

    Dong et al Nat Biotechnol. 2021 Nov 11. pii: 10.103

    Insert

    Endocannabinoid sensor GRAB_eCB2.0
    

    Use

    AAV

    Mutation

    S383T

    Promoter

    hSyn

    Available Since

    Feb. 18, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCMVHA EED wt

    Plasmid

    #24231

    Purpose

    Mammalian expression of human EED with HA tag

    Depositor

    Kristian Helin

    Article

    Bracken et al EMBO J. 2003 Oct 15. 22(20):5323-35.

    Insert

    EED (EED Human)
    

    Tags

    HA

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Jan. 25, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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18. pcDNA3.4_CEACAM6-His

    Plasmid

    #149337

    Purpose

    for eukaryotic expression and purification of CEACAM6-His6

    Depositor

    James Fleckenstein

    Article

    Sheikh et al Proc Natl Acad Sci U S A. 2020 Nov 17;11

    Insert

    CEACAM6 (CEACAM6 Human)
    

    Tags

    IL-2 signal peptide and polyhistidine

    Expression

    Mammalian

    Mutation

    point mutation (GGA to GCA) to replace 'G-g…

    Promoter

    CMV

    Available Since

    Oct. 28, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. pBMN-YFP-Parkin

    Plasmid

    #59416

    Purpose

    to make YFP-Parkin stable cell line with retrovirus

    Depositor

    Richard Youle

    Article

    Yamano et al Elife (Cambridge). 2014 Feb 25;3:e01612.

    Insert

    YFP-Parkin (PRKN Human)
    

    Use

    Retroviral

    Tags

    EYFP

    Expression

    Mammalian

    Available Since

    Aug. 22, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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20. pcDNA3.1_3xFlagEzh2 WT

    Plasmid

    #173717

    Purpose

    WT EZH2 expression plasmid with 3XFLAG tag

    Depositor

    Thomas Cech

    Article

    Long et al Nat Genet. 2020 Sep;52(9):931-938. doi:

    Insert

    EZH2 (EZH2 Human)
    

    Tags

    3XFLAG

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Aug. 26, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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