Skip to main content
Addgene
Open Menu

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

Information

In Category

Filter By

More Filters

We narrowed to 65,871 results for: des.1

Clear keywords
Showing: 2741 - 2760 of 65871 results

  3. pAAV-EF1a-N-CretrcintG

    Plasmid
    #69570
    Purpose
    One half component of the optimized Cre recombinase dependent on GFP (CRE-DOG OPT) for rAAV production and expression in mammalian cells
    Depositor
    Connie Cepko
    Article
    Tang et al Nat Neurosci. 2015 Sep;18(9):1334-41. do
    Has Service
    AAV1
    Insert
    kozak consensus - N-CretrcintG
    Use
    AAV and Synthetic Biology
    Expression
    Mammalian
    Promoter
    EF1a
    Available Since
    Oct. 21, 2015
    Availability
    Academic Institutions and Nonprofits only
    69570-plasmid-map-sequence-id-127742

    Enlarge

  4. pRosetta

    Plasmid
    #59700
    Purpose
    Lentiviral vector for use as a standard for titering experiments when trying to compare vectors with different selection markers or to determine appropriate antibiotic conditions.
    Depositor
    John Doench, David Root
    Inserts
    Puro
    BlastR
    EGFP
    Use
    Lentiviral
    Tags
    2A
    Expression
    Mammalian
    Available Since
    Sept. 3, 2014
    Availability
    Academic Institutions and Nonprofits only
    59700-plasmid-map-sequence-id-140560

    Enlarge

  7. GLP1R-DuET

    Plasmid
    #213253
    Purpose
    The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.
    Depositor
    Thomas Sakmar
    Article
    Dahl et al Sci Adv. 2023 May 3;9(18):eadf9297. doi:
    Insert
    GLP1R (GLP1R Human)
    Tags
    1D4 and FLAG
    Expression
    Mammalian
    Promoter
    CMV, T7
    Available Since
    April 23, 2024
    Availability
    Academic Institutions and Nonprofits only
    213253-plasmid-map-sequence-id-430803

    Enlarge

  14. CoV2-Spike-D614G

    Plasmid
    #177960
    Purpose
    Transient mammalian expression of codon optimized SARS-CoV-2 Spike protein (original Wuhan Hu1 sequence + D614G mutation)
    Depositor
    Jennifer Doudna
    Article
    Syed et al Science. 2021 Nov 4:eabl6184. doi: 10.11
    Insert
    SARS-CoV-2 Spike protein (original Wuhan Hu1 sequence + D614G mutation) (S )
    Expression
    Mammalian
    Mutation
    D614G
    Available Since
    Nov. 17, 2021
    Availability
    Academic Institutions and Nonprofits only
    177960-plasmid-map-sequence-id-348179

    Enlarge

  18. PB-SAM

    Plasmid
    #102559
    Purpose
    Piggybac transposon vector encoding dCAS9-VP64 and MS2-P65-HSF1 activator helper complex.
    Depositor
    Ying Liu
    Article
    Li et al Mol Ther Nucleic Acids. 2017 Sep 15;8:64
    Inserts
    MS2-P65-HSF1_T2A_Hygro
    dCAS9(D10A, N863A)-VP64_T2A_Blast
    Use
    CRISPR; Piggybac transposon
    Expression
    Mammalian
    Mutation
    D10A and N863A in Cas9 and N55K in MS2
    Promoter
    CAG and EF1A
    Available Since
    Jan. 5, 2018
    Availability
    Academic Institutions and Nonprofits only
    102559-plasmid-map-sequence-id-193885

    Enlarge

Showing: 2741 - 2760 of 65871 results