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We narrowed to 30,644 results for: PLE

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Showing: 4141 - 4170 of 30644 results

  4. pSLiP-G2

    Plasmid
    #188964
    Purpose
    L-Rhamnose inducible catalytically inactive Cas9 with sgRNA for bacterial gene knockdown
    Depositor
    Seung-Goo Lee
    Article
    Kim et al Nucleic Acids Res. 2022 Dec 9;50(22):131
    Inserts
    dCas9
    sgRNA: gttagacgctgattacatggactagg
    Use
    Synthetic Biology
    Expression
    Bacterial
    Promoter
    PrhaBAD
    Available Since
    Sept. 13, 2022
    Availability
    Academic Institutions and Nonprofits only
    188964-plasmid-map-sequence-id-371924

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  7. pSWD95Y14D

    Plasmid
    #159098
    Purpose
    CckA FRET Sensor with cyclic-di-GMP point mutant
    Depositor
    Seth Childers
    Article
    Duvall et al ACS Sens. 2020 Jun 26;5(6):1589-1596. do
    Insert
    CckA 1-182, Linker SNVTRHRSATE, mClover3, CckA 183-691(Y514D), Linker SNVTRHRSAT, mRuby3
    Expression
    Bacterial
    Mutation
    Mutated Y514 to D514
    Promoter
    Xylose
    Available Since
    Nov. 17, 2020
    Availability
    Academic Institutions and Nonprofits only
    159098-plasmid-map-sequence-id-310723

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  8. pSLQ8636 PB-PGK-RR12EE345L-C term (406) dLbCpf1-N intein-pA

    Plasmid
    #140223
    Purpose
    Constitutive PGK driven expression of leucine zipper with C terminal dCpf1 (Cas12a) 406-split half fused to intein for 3-way split activator
    Depositor
    Stanley Qi
    Article
    Kempton et al Mol Cell. 2020 Jan 30. pii: S1097-2765(2
    Insert
    zipper with dLbCpf1 (Cas12a) C terminal half (406) fused to N-intein
    Expression
    Mammalian
    Mutation
    D832A
    Promoter
    PGK
    Available Since
    April 21, 2020
    Availability
    Academic Institutions and Nonprofits only
    140223-plasmid-map-sequence-id-292003

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  10. pXW109ABS62(RS)

    Plasmid
    #123153
    Purpose
    Amplified arsenic sensor with double ArsR binding sites. pSB3K3 carrying J109-30arsR-t-ParsR-ABS62-30hrpR-30hrpS-t-PhrpLE-30gfp-t
    Depositor
    Baojun Wang
    Article
    Wan et al Nat Chem Biol. 2019 May;15(5):540-548. d
    Insert
    J109-30arsR-t-ParsR-ABS62-30hrpR-30hrpS-t-PhrpLE-30gfp-t
    Use
    Synthetic Biology
    Expression
    Bacterial
    Promoter
    J109, ParsR-ABS62, PhrpLE
    Available Since
    May 9, 2019
    Availability
    Academic Institutions and Nonprofits only
    123153-plasmid-map-sequence-id-241974

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  11. pXW109ABS67(RS)

    Plasmid
    #123154
    Purpose
    Amplified arsenic sensor with double ArsR binding sites. pSB3K3 carrying J109-30arsR-t-ParsR-ABS67-30hrpR-30hrpS-t-PhrpLE-30gfp-t
    Depositor
    Baojun Wang
    Article
    Wan et al Nat Chem Biol. 2019 May;15(5):540-548. d
    Insert
    J109-30arsR-t-ParsR-ABS67-30hrpR-30hrpS-t-PhrpLE-30gfp-t
    Use
    Synthetic Biology
    Expression
    Bacterial
    Promoter
    J109. ParsR-ABS67, PhrpLE
    Available Since
    May 9, 2019
    Availability
    Academic Institutions and Nonprofits only
    123154-plasmid-map-sequence-id-240629

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  18. pVH009

    Plasmid
    #80399
    Purpose
    Control plasmid with no anti-scaffold feedback. Contains pBAD-CusR (response regulator) and pCusR-YFP-AAV.
    Depositor
    Richard Murray
    Article
    Hsiao et al ACS Synth Biol. 2015 Feb 20;4(2):150-61.
    Inserts
    3xFLAG-Venus YFP-AAV
    CusR (cusR )
    AraC (araC )
    Use
    Synthetic Biology
    Tags
    C-terminal LZx domain and N-terminal 3xFLAG tag, …
    Expression
    Bacterial
    Available Since
    Sept. 20, 2016
    Availability
    Academic Institutions and Nonprofits only
    80399-plasmid-map-sequence-id-155900

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  22. lenti dCAS-VP64_Blast

    Plasmid
    #61425
    Purpose
    3rd generation lenti vector encoding dCAS9-VP64 with 2A Blast resistance marker (EF1a-NLS-dCas9(N863)-VP64-2A-Blast-WPRE)
    Depositor
    Feng Zhang
    Article
    Konermann et al Nature. 2014 Dec 10. doi: 10.1038/nature
    Has Service
    Lentiviral Prep
    Insert
    dCAS9(D10A, N863A)-VP64_2A_Blast
    Use
    CRISPR and Lentiviral
    Expression
    Mammalian
    Mutation
    D10A and N863A in Cas9
    Promoter
    EF1A
    Available Since
    Dec. 19, 2014
    Availability
    Academic Institutions and Nonprofits only
    61425-plasmid-map-sequence-id-457486

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  25. pSR657

    Plasmid
    #65214
    Purpose
    Baculovirus-mediated rAAV production: Expresses bicistronic AAV-2 rep gene and polycistronic AAV-2 cap gene in Sf9 insect cells
    Depositor
    Robert Kotin
    Article
    Smith et al Mol Ther. 2009 Nov;17(11):1888-96. doi:
    Inserts
    modified AAV-2 rep78
    modified AAV-2 cap
    Use
    AAV
    Expression
    Insect
    Promoter
    p10 and polyhedrin
    Available Since
    June 17, 2015
    Availability
    Academic Institutions and Nonprofits only
    65214-plasmid-map-sequence-id-116341

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  27. pSL1765 (pSPIN-R, R*MmeI)

    Plasmid
    #160734
    Purpose
    Single-plasmid V. cholerae CAST. Encodes all proteins, crRNA, donor DNA; non-targeting crRNA with BsaI sites. Reverse (R) order minimizes self-targeting; MmeI site for Tn-seq; pBBR1 backbone.
    Depositor
    Samuel H. Sternberg
    Article
    Vo et al Nat Biotechnol. 2020 Nov 23. pii: 10.103
    Insert
    VchCAST proteins, crRNA, and donor DNA
    Expression
    Bacterial
    Promoter
    J23119
    Available Since
    Dec. 1, 2020
    Availability
    Academic Institutions and Nonprofits only
    160734-plasmid-map-sequence-id-318595

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  28. HIV-1 LTR-gfp

    Plasmid
    #115809
    Purpose
    HIV-1 LTR driven reporter vector that retains complete LTRs, tat, and rev, but has a frameshift mutation in env, an ngfr reporter gene in place of nef, and gfp in place of gag, pol, vif, and vpr
    Depositor
    Jeremy Luban
    Article
    Yurkovetskiy et al Nat Microbiol. 2018 Dec;3(12):1354-1361.
    Insert
    gfp and ngfr
    Use
    Lentiviral
    Promoter
    HIV1 LTR
    Available Since
    Feb. 14, 2019
    Availability
    Academic Institutions and Nonprofits only
    115809-plasmid-map-sequence-id-232377

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  29. pJRH051

    Plasmid
    #171625
    Purpose
    2nd generation lentiviral transfer plasmid encoding a U6 promoter expressing a spyCas9 sgRNA (no spacer, BsmBI cutsites) and an EF1-a promoter expressing mNeonGreen
    Depositor
    Jennifer Doudna
    Article
    Hamilton et al Cell Rep. 2021 Jun 1;35(9):109207. doi:
    Inserts
    spyCas9 sgRNA-BsmBI-Destination
    mNeon
    Use
    CRISPR and Lentiviral
    Expression
    Mammalian
    Promoter
    EF1-a and U6
    Available Since
    June 28, 2021
    Availability
    Academic Institutions and Nonprofits only
    171625-plasmid-map-sequence-id-335505

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  30. pCAT003

    Plasmid
    #171628
    Purpose
    2nd generation lentiviral transfer plasmid encoding a U6 promoter expressing a spyCas9 sgRNA targeting TRAC and an EF1-a promoter expressing mNeonGreen
    Depositor
    Jennifer Doudna
    Article
    Hamilton et al Cell Rep. 2021 Jun 1;35(9):109207. doi:
    Inserts
    spyCas9 sgRNA-TRAC
    mNeon
    Use
    CRISPR and Lentiviral
    Expression
    Mammalian
    Promoter
    CAG and EF1-a
    Available Since
    June 30, 2021
    Availability
    Academic Institutions and Nonprofits only
    171628-plasmid-map-sequence-id-336485

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Showing: 4141 - 4170 of 30644 results