We narrowed to 17,000 results for: por

1. pY2-LYS-IV

   Plasmid

   #218118

   Purpose

   LYS-Leu2 plasmid (218114) with the functional cassettes of pY2 (218115) inserted at the sfGFP dropout locus.

   Depositor

   Carl Denard

   Article

   Martinusen et al Protein Eng Des Sel. 2024 Jan 29;37:gzae

   Type

   Empty backbone

   Expression

   Bacterial and Yeast

   Available Since

   April 29, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. peGFP-N1-SHB

   Plasmid

   #139605

   Purpose

   Expresses eGFP-SHB fusion protein in mammalian cells

   Depositor

   Michael Welsh

   Article

   Pietila et al Cells. 2019 Dec 15;8(12). pii: cells8121

   Insert

   SHB (SHB Human)
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   April 17, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. pcDNA3.1(-) FLIPQTV3.0 2m

   Plasmid

   #63731

   Purpose

   FLIPQTV3.0 glutamine sensor with R75MW220A mutations

   Depositor

   Sakiko Okumoto

   Article

   Gruenwald et al PLoS One. 2012;7(6):e38591. doi: 10.1371

   Insert

   FLIPQTV3.0 sensor, 2mM affinity
   

   Expression

   Mammalian

   Available Since

   Nov. 18, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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4. 6xO/S luc

   Plasmid

   #69445

   Purpose

   detection/assessment of OCT4 and SOX2 transcription factor activity

   Depositor

   Lisa Dailey

   Insert

   minimal fgf4 promoter and 6XOS tandem repeat of composite OCT4/Sox2 binding site from FGF4 enhancer
   

   Use

   Luciferase

   Expression

   Mammalian

   Available Since

   May 5, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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5. pEutRwc

   Plasmid

   #216334

   Purpose

   An IPTG-inducible promoter controlling expression of eutR; PEutS, extended controlling expression of sfgfp

   Depositor

   Mark Styczynski

   Article

   McNerney et al ACS Synth Biol. 2020 Feb 21;9(2):402-411

   Inserts

   EutR
   Superfolder green fluorescent protein
   

   Expression

   Bacterial

   Available Since

   Dec. 23, 2024

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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6. env8 reverse J1/13

   Plasmid

   #99831

   Purpose

   GFPuv reporter that places expression of a fluorescent protein reporter (GFPuv) under control of the env8HyCbl riboswitch.

   Depositor

   Robert Batey

   Article

   Polaski et al Cell Rep. 2016 May 3;15(5):1100-10. doi:

   Insert

   env8 reverse J1/13
   

   Tags

   GFPuv

   Expression

   Bacterial

   Mutation

   J1/13 sequence is reversed

   Available Since

   Aug. 25, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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7. pRRL-GFPAdGO-PGK-Neo

   Plasmid

   #136898

   Purpose

   Lentivirus for base editing activatable GFP expression in mammalian cells

   Depositor

   Lukas Dow

   Article

   Katti et al Nucleic Acids Res. 2020 Feb 29. pii: 576

   Insert

   GFPAdGO
   

   Use

   Lentiviral

   Tags

   2xNLS

   Mutation

   TGG>TGA

   Promoter

   SFFV

   Available Since

   Feb. 10, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. pAV0679

   Plasmid

   #149689

   Purpose

   Bacterial expression of SENP_EuH protease

   Depositor

   Dirk Görlich

   Article

   Vera Rodriguez et al J Cell Biol. 2019 Jun 3;218(6):2006-2020

   Insert

   SENP_EuH protease
   

   Tags

   His14-tag and Tev protease cleavage site

   Expression

   Bacterial

   Available Since

   July 20, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. pActP-dtrpA1

   Plasmid

   #64713

   Purpose

   inducing calcium influx in cultured Drosophila S2 cells

   Depositor

   Liqun Luo

   Article

   Gao et al Nat Neurosci. 2015 May 11. doi: 10.1038/

   Insert

   dtrpA1 (TrpA1 )
   

   Expression

   Insect

   Available Since

   May 7, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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10. JGE302_pAAV-hSyn-DIO-Myc-PYLcs-bArrestin2-YFP (p2)

    Plasmid

    #107247

    Purpose

    beta arrestin 2 fused to PYLcs for abscisic acid mediated recruitment of ABIcs labelled proteins.

    Depositor

    Bryan Roth

    Article

    Roth et al bioRxiv 251769

    Insert

    ARRB2
    

    Use

    AAV

    Tags

    MYC, PYLcs, and YFP

    Promoter

    hSYN

    Available Since

    April 26, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCR8-attR4-pegRNA-attR3

    Plasmid

    #213056

    Purpose

    Modular Unit carrying pegRNA and ngRNA scaffolds flanked by attR4 and attR3

    Depositor

    Bing Yang

    Article

    Gupta et al Plant Commun. 2023 Oct 26:100741. doi: 1

    Insert

    sgRNA
    

    Expression

    Bacterial

    Promoter

    35S-CmYCLV-AtU6

    Available Since

    Feb. 7, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCR8-attL5-pegRNA-attL4

    Plasmid

    #213055

    Purpose

    Modular Unit carrying pegRNA and ngRNA scaffolds flanked by attL5 and attL4

    Depositor

    Bing Yang

    Article

    Gupta et al Plant Commun. 2023 Oct 26:100741. doi: 1

    Insert

    sgRNA
    

    Expression

    Bacterial

    Promoter

    35S-CmYCLV-AtU6

    Available Since

    Feb. 7, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCR8-attL1-pegRNA-attR5

    Plasmid

    #213053

    Purpose

    Modular Unit carrying pegRNA and ngRNA scaffolds flanked by attL1 and attR5

    Depositor

    Bing Yang

    Article

    Gupta et al Plant Commun. 2023 Oct 26:100741. doi: 1

    Insert

    sgRNA
    

    Expression

    Bacterial

    Promoter

    35S-CmYCLV-AtU6

    Available Since

    Feb. 7, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. pEX.sEF1a.Kozak.HA.NLS.Sce.T2A.BFP

    Plasmid

    #45565

    Purpose

    Expresses I-Sce I nuclease in mammalian cells and BFP as a tracker

    Depositor

    Andrew Scharenberg

    Article

    Kuhar et al Nucleic Acids Res. 2013 Oct 10.

    Inserts

    I-Sce I Nuclease
    BFP
    

    Tags

    HA

    Expression

    Mammalian

    Available Since

    Nov. 4, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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15. pRK5_tAKAR4α

    Plasmid

    #119919

    Purpose

    Mammalian expression of the tAKAR4α sensor for detecting PKA activity using ratiometric imaging

    Depositor

    Haining Zhong

    Article

    Ma et al Neuron. 2018 Aug 22;99(4):665-679.e5. do

    Insert

    tAKAR4α
    

    Expression

    Mammalian

    Available Since

    March 9, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. pKM197

    Plasmid

    #132665

    Purpose

    pyrE-targeted CRISPR-Cas9 control plasmid. Cas9 expression is controlled by the xylR promoter and results in improved conjugation efficiency. Induction with xylose results in a pyrE mutant.

    Depositor

    Joseph Sorg

    Article

    Bhattacharjee et al mSphere. 2020 Mar 4;5(2):e00941-19. doi:

    Insert

    Upstream & Downstream pyrE deletion region
    

    Use

    E. coli - c. difficile shuttle vector

    Available Since

    Oct. 17, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. HA-BICD2(1-500)-FRB

    Plasmid

    #174648

    Purpose

    Rapalog-inducible coupling to dynein adaptor BICD2

    Depositor

    Lukas Kapitein

    Article

    Nijenhuis et al J Cell Biol. 2020 Apr 6;219(4). pii: 133

    Insert

    HA-BICD2(1-500)-FRB
    

    Tags

    FRB and HA

    Expression

    Mammalian

    Mutation

    mmBICD2(aa1-500): Lys213Glu

    Promoter

    Chicken beta-actin

    Available Since

    Nov. 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. p404-MTS-mCherry-GFP1-10

    Plasmid

    #91958

    Purpose

    Expresses mCherry-GFP1-10 in yeast cell mitochondria

    Depositor

    Rong Li

    Article

    Ruan et al Nature. 2017 Mar 16;543(7645):443-446. d

    Insert

    MTS-mCherry-GFP1-10
    

    Expression

    Yeast

    Promoter

    GAP

    Available Since

    June 7, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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19. pRR-PR-5Z

    Plasmid

    #23057

    Depositor

    Charles Miller

    Article

    Miller CA et al Plasmid. 2010 Mar;63(2):73-8. doi: 10.10

    Insert

    PR (PGR Human)
    

    Expression

    Yeast

    Available Since

    Jan. 27, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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20. MK1283

    Plasmid

    #71428

    Purpose

    This E. coli DH10B strain harbors a shuttle vector plasmid that expresses the Mixed Feedback Loop version of the UBER system with a T7RNAP translation rate of 1535 and a TetR translation rate of 30709

    Depositor

    Howard Salis

    Article

    Kushwaha et al Nat Commun. 2015 Jul 17;6:7832. doi: 10.

    Insert

    Mixed Feedback Loop version of the UBER system
    

    Mutation

    T7 RBS: AACCGAGCCCAATATAGGACCTAGGGTGCCAAAAAA and …

    Available Since

    Feb. 19, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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21. pCHW110032

    Plasmid

    #222438

    Purpose

    Level 0, promoter module (GGAG-AATG) containing CBS4 and CMV1 promoters.

    Depositor

    Chih-Hang Wu

    Article

    Chiang et al bioRxiv 2024.03.23.586378

    Insert

    CBS4-CMV1, , CBS4 promoter + CMV1 (Cucumber mosaic virus)
    

    Use

    Synthetic Biology

    Expression

    Plant

    Mutation

    no

    Available Since

    Nov. 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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22. pCHW00011

    Plasmid

    #222439

    Purpose

    Level 0, CDSns module (AATG-TTCG) containing S. cerevisiae CUP2.

    Depositor

    Chih-Hang Wu

    Article

    Chiang et al bioRxiv 2024.03.23.586378

    Insert

    CUP2 (Saccharomyces cerevisiae) (CUP2 Budding Yeast)
    

    Use

    Synthetic Biology

    Expression

    Plant

    Mutation

    no

    Available Since

    Nov. 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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23. pcDNA3-cdc2-AF (718)

    Plasmid

    #39872

    Depositor

    Jonathon Pines

    Article

    Hagting et al EMBO J. 1998 Jul 15;17(14):4127-38.

    Insert

    Cdc2 (CDK1 Human)
    

    Expression

    Mammalian

    Mutation

    AF = T14A, Y15F

    Promoter

    CMV

    Available Since

    June 8, 2015

    Availability

    Academic Institutions and Nonprofits only

24. pCMV10-3xFLAG-prs-XK

    Plasmid

    #184369

    Purpose

    Express human XK in Expi293 cells

    Depositor

    Karin Reinisch

    Article

    Adlakha et al J Cell Biol. 2022 Jun 6;221(5):e20220203

    Insert

    Human XK (XK Human)
    

    Tags

    3xFLAG-PreScission cleavage site

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    June 29, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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25. pCHARGE3

    Plasmid

    #24658

    Depositor

    Tanya Parish

    Article

    Carroll et al PLoS One. 2010 . 5(3):e9823.

    Insert

    Turbo
    

    Expression

    Bacterial

    Available Since

    June 9, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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26. pPD545 ZF37x6-Compact_EYFP

    Plasmid

    #138859

    Purpose

    EYFP reporter vector for the ZF37x6-Compact promoter

    Depositor

    Joshua Leonard

    Article

    Donahue et al Nat Commun. 2020 Feb 7;11(1):779. doi: 1

    Insert

    ZF37x6-Compact
    

    Use

    Synthetic Biology

    Expression

    Mammalian

    Promoter

    ZF37x6-Compact

    Available Since

    Jan. 21, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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27. pcDNA3.1(-) FLIPQTV3.0 8m

    Plasmid

    #63732

    Purpose

    FLIPQTV3.0 glutamine sensor with R75MY86A mutations

    Depositor

    Sakiko Okumoto

    Article

    Gruenwald et al PLoS One. 2012;7(6):e38591. doi: 10.1371

    Insert

    FLIPQTV3.0 sensor, 8mM affinity
    

    Expression

    Mammalian

    Available Since

    Nov. 18, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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28. pPD558 ZF172x6-Compact_EYFP

    Plasmid

    #138872

    Purpose

    EYFP reporter vector for the ZF172x6-Compact promoter

    Depositor

    Joshua Leonard

    Article

    Donahue et al Nat Commun. 2020 Feb 7;11(1):779. doi: 1

    Insert

    ZF172x6-Compact
    

    Use

    Synthetic Biology

    Expression

    Mammalian

    Promoter

    ZF172x6-Compact

    Available Since

    Jan. 26, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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29. pNM262

    Plasmid

    #128412

    Purpose

    Vanillic acid inducible production of lysostaphin. LsyT with the amyE signaling peptide for secretion.

    Depositor

    Christopher Voigt

    Article

    Gonzalez et al Nat Chem Biol. 2019 Dec 2. pii: 10.1038/

    Insert

    Lysostaphin (epr Staphylococcus simulans)
    

    Use

    Synthetic Biology

    Tags

    amyE SP

    Expression

    Bacterial

    Promoter

    Pspank(V)

    Available Since

    Sept. 2, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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30. pGP-FB-orig BA

    Plasmid

    #13420

    Depositor

    Keith Joung

    Article

    Wright et al Nat Protoc. 2006;1(3):1637-52. doi: 10.1

    Insert

    orig BA
    

    Tags

    Gal11P

    Expression

    Bacterial

    Available Since

    Feb. 8, 2007

    Availability

    Academic Institutions and Nonprofits only

    

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