We narrowed to 11,551 results for: NSI

1. pLPC-MYC.Mm.RAP1.delta.MYB

   Plasmid

   #184660

   Purpose

   Retroviral expression of mouse RAP1 delta MYB

   Depositor

   Agnel Sfeir

   Article

   Barry et al Genes Dev. 2022 Feb 24. pii: gad.349039.

   Insert

   rap1
   

   Use

   Retroviral

   Tags

   MYC

   Expression

   Mammalian

   Mutation

   rap1 delta MYB

   Promoter

   CMV

   Available Since

   May 24, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pLPC-MYC.Mm.RAP1.delta.COIL

   Plasmid

   #184659

   Purpose

   Retroviral expression of mouse RAP1 delta COIL

   Depositor

   Agnel Sfeir

   Article

   Barry et al Genes Dev. 2022 Feb 24. pii: gad.349039.

   Insert

   pLPC
   

   Use

   Retroviral

   Tags

   MYC

   Expression

   Mammalian

   Mutation

   rap1 delta COIL

   Promoter

   CMV

   Available Since

   May 24, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pLPC-MYC.Mm.RAP1.delta.BRCT

   Plasmid

   #184658

   Purpose

   Retroviral expression of mouse Rap1 delta BRCT

   Depositor

   Agnel Sfeir

   Article

   Barry et al Genes Dev. 2022 Feb 24. pii: gad.349039.

   Insert

   RAP1
   

   Use

   Retroviral

   Tags

   MYC

   Expression

   Mammalian

   Mutation

   rap1 delta BRCT

   Promoter

   CMV

   Available Since

   May 24, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pLPC-MYC.Mm.RAP1.delta.RCT

   Plasmid

   #184661

   Purpose

   Retroviral expression of mouse RAP1 delta RCT

   Depositor

   Agnel Sfeir

   Article

   Barry et al Genes Dev. 2022 Feb 24. pii: gad.349039.

   Insert

   rap1
   

   Use

   Retroviral

   Tags

   MYC

   Expression

   Mammalian

   Mutation

   rap1 delta RCT

   Promoter

   CMV

   Available Since

   May 24, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pHAGE-IRES-puro-NLS-dHgm4Cas13b-EGFP-NLS

   Plasmid

   #191360

   Purpose

   Overexpression of NLS-dHgm4Cas13b-EGFP-NLS in human cells

   Depositor

   Ling-Ling Chen

   Article

   Yang et al Cell Insight. 2022 Jun 15;1(4):100044. d

   Insert

   NLS-dHgm4Cas13b-EGFP-NLS
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Oct. 25, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pcDNA3.1-R2ACR_142-EYFP

   Plasmid

   #103964

   Purpose

   Highly efficient anion channelrhodopsin from Rhodomonas sp. (CCAC1630) for expression in mammalian cells

   Depositor

   John Spudich

   Article

   Govorunova et al Sci Rep. 2017 Mar 3;7:43358. doi: 10.103

   Insert

   Anion channelrhodopsin R2ACR_142
   

   Use

   Tags

   EYFP

   Expression

   Mammalian

   Mutation

   human codon optimized

   Promoter

   CMV

   Available Since

   Nov. 30, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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7. pHH0103_STAC3-2/2

   Plasmid

   #91466

   Purpose

   Protein expression and purification of human SH3 domain construct STAC3-2/2

   Depositor

   Sachdev Sidhu

   Article

   Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

   Insert

   STAC3-2/2 (STAC3 Human)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   March 19, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pHH0103_SH3GL1-1/1

   Plasmid

   #91473

   Purpose

   Protein expression and purification of human SH3 domain construct SH3GL1-1/1

   Depositor

   Sachdev Sidhu

   Article

   Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

   Insert

   SH3GL1-1/1 (SH3GL1 Human)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   March 19, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pHH0103_VAV2-1/2*

   Plasmid

   #91483

   Purpose

   Protein expression and purification of human SH3 domain construct VAV2-1/2*

   Depositor

   Sachdev Sidhu

   Article

   Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

   Insert

   VAV2-1/2* (VAV2 Human)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   March 19, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pHH0103_STAC3-1/2

    Plasmid

    #91271

    Purpose

    Protein expression and purification of human SH3 domain construct STAC3-1/2

    Depositor

    Sachdev Sidhu

    Article

    Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

    Insert

    STAC3-1/2 (STAC3 Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    March 19, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. pBad-CA TAG93

    Plasmid

    #105667

    Purpose

    expresses thermostable variant of human carbonic anhydrase II with TAG stop codon at site 93 and C-terminal 6x his tag

    Depositor

    Ryan Mehl

    Article

    Mehl et al 2020

    Insert

    human carbonic anhydrase II thermostable variant (CA2 Human)
    

    Use

    Tags

    6x his tag

    Expression

    Bacterial

    Mutation

    TAG stop codon at site 93

    Promoter

    pBad/arabinose

    Available Since

    March 14, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. pET21a(+) LdtMt2 (131-408)

    Plasmid

    #59337

    Purpose

    Expresses LdtMt2 for L,D-transpeptidation

    Depositor

    SeWon Suh

    Article

    Kim et al Acta Crystallogr D Biol Crystallogr. 201

    Insert

    LdtMt2 (131-408)
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Nov. 5, 2014

    Availability

    Academic Institutions and Nonprofits only

13. pY71-tet.I.8

    Plasmid

    #133521

    Purpose

    sfGFP driven by a T7 promoter with the tetO sequence 11 bp downstream from the promoter start site

    Depositor

    Richard Murray

    Article

    McManus et al Arch Biochem Biophys. 2019 Jul 18. pii:

    Insert

    sfGFP
    

    Use

    Tags

    Strep tag and TEV site

    Expression

    Bacterial

    Mutation

    WT

    Promoter

    T7

    Available Since

    Dec. 12, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. pEPQD1CB0770

    Plasmid

    #177060

    Purpose

    MoClo Level 1, position 5, transcriptional unit for transient expression of loganic acid O-methyltransferase (CrLAMT) from Catharanthus roseus driven by 35S promoter; contains a chloroplast transit peptide

    Depositor

    Nicola Patron

    Article

    Dudley et al Commun Biol. 2022 Sep 10;5(1):949. doi:

    Insert

    loganic acid O-methyltransferase (CrLAMT) from Catharanthus roseus
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Dec. 6, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pDT-sgRNA-XMAS-2x

    Plasmid

    #164414

    Purpose

    Dual targeted guide and cloning vector. Targets pEF-XMAS-2xStop. Guides targeting endogenous sites can be cloned into BbsI sites.

    Depositor

    Xiao Wang

    Article

    Brookhouser et al BMC Biol. 2020 Dec 14;18(1):193. doi: 10

    Insert

    sgRNA
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available Since

    Aug. 4, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pHH0103_SPTAN1-1/1

    Plasmid

    #91292

    Purpose

    Protein expression and purification of human SH3 domain construct SPTAN1-1/1

    Depositor

    Sachdev Sidhu

    Article

    Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

    Insert

    SPTAN1-1/1 (SPTAN1 Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    March 19, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pmalc4x_I499F_TEV

    Plasmid

    #47703

    Purpose

    Expresses MBP myocilin olfactomedin domain fusion protein with a TEV protease recognition sequence and the mutation I499F

    Depositor

    Raquel Lieberman

    Article

    Burns et al Biochemistry. 2011 Jul 5;50(26):5824-33.

    Insert

    Olfactomedin-like domain with I499F mutation (MYOC Human)
    

    Use

    Tags

    MBP

    Expression

    Bacterial

    Mutation

    changed I499F

    Promoter

    Ptac

    Available Since

    Sept. 9, 2013

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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18. pAAV-flex-rev-g2-2A-Venus

    Plasmid

    #71410

    Purpose

    This plasmid is pAAV-panpromoter-flex-rev-gamma2F77-2A-Venus. Confers zolpidem sensitivity on zolpidem-insensitive neurons.

    Depositor

    William Wisden

    Article

    Uygun et al J Neurosci. 2016 Nov 2;36(44):11171-1118

    Inserts

    Use

    AAV and Cre/Lox

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    mouse hdc gene promoter, works as a pan neuronal …

    Available Since

    March 1, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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19. pIBA3-SpyCatcherEQ-sfGFP

    Plasmid

    #107421

    Purpose

    For production of inactive SpyCatcherEQ protein fused N-terminally to sfGFP. Includes N-terminal His tag for purification and C-terminal StrepII tag for detection.

    Depositor

    Jack Leo

    Article

    Chauhan et al Mol Microbiol. 2019 Jan 1. doi: 10.1111/

    Insert

    SpyCatcher
    

    Use

    Tags

    His tag, StrepII, and superfolder GFP

    Expression

    Bacterial

    Mutation

    mutation of glutamate 77 to glutamine

    Promoter

    Tet

    Available Since

    Jan. 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pHH0103_SNX33-1/1

    Plasmid

    #91278

    Purpose

    Protein expression and purification of human SH3 domain construct SNX33-1/1

    Depositor

    Sachdev Sidhu

    Article

    Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

    Insert

    SNX33-1/1 (SNX33 Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    March 19, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pEPQD1CB0109

    Plasmid

    #177042

    Purpose

    MoClo Level 1, position 2, transcriptional unit for transient expression of geranyl pyrophosphate synthase (PaGPPS1) from Picea abies driven by 35S promoter; contains a chloroplast transit peptide

    Depositor

    Nicola Patron

    Article

    Dudley et al Commun Biol. 2022 Sep 10;5(1):949. doi:

    Insert

    geranyl pyrophosphate synthase (PaGPPS1) from Picea abies
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Nov. 30, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pCS2-zfKitlgb

    Plasmid

    #168200

    Purpose

    Express zebrafish Kitlgb, mRNA synthesis

    Depositor

    Petr Bartunek

    Article

    Oltova et al Blood Adv. 2020 Dec 8;4(23):5915-5924. d

    Insert

    Kitlgb (kitlgb Zebrafish)
    

    Use

    Vertebrate expression (zebrafish, chicken, xenopu…

    Tags

    Expression

    Mammalian

    Mutation

    I85V, V222I, and D233N- please see depositor comm…

    Promoter

    Available Since

    Nov. 3, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. 3xEGFP-mMeikin (E156R, R159E)

    Plasmid

    #174710

    Purpose

    Mammalian expression of mouse Meikin with Separase-resistant mutations

    Depositor

    Iain Cheeseman

    Article

    Maier et al Dev Cell. 2021 Aug 9;56(15):2192-2206.e8

    Insert

    Meikin (Meikin Mouse)
    

    Use

    Tags

    3xEGFP

    Expression

    Mammalian

    Mutation

    E156R, R159E

    Promoter

    Available Since

    Sept. 22, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pIC389

    Plasmid

    #37387

    Depositor

    Iain Cheeseman

    Article

    Kiyomitsu et al Nat Cell Biol. 2012 Feb 12;14(3):311-7.

    Insert

    LGN (GPSM2 Human)
    

    Use

    Retroviral

    Tags

    GFP, S-tag, and TEV

    Expression

    Mammalian

    Mutation

    deleted amino acid 1-364 and 524

    Promoter

    Available Since

    July 19, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pET_RGG-RGG_withTEVsite

    Plasmid

    #124940

    Purpose

    Expression of RGG-RGG

    Depositor

    Benjamin Schuster, Matthew Good, Daniel Hammer

    Article

    Schuster et al Nat Commun. 2018 Jul 30;9(1):2985. doi:

    Insert

    RGG-RGG (using LAF-1 RGG domain) (laf-1 Nematode)
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    with TEV site between RGG domains

    Promoter

    Available Since

    May 29, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. MSCV-miRE_shBRD9_508-SV40-GFP

    Plasmid

    #75138

    Purpose

    Retroviral expression plasmid encoding shBRD9_508 (targeting human BRD)

    Depositor

    Christopher Vakoc

    Article

    Hohmann et al Nat Chem Biol. 2016 Jul 4. doi: 10.1038/

    Insert

    shBRD9_508
    

    Use

    Retroviral

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    May 23, 2016

    Availability

    Academic Institutions and Nonprofits only

27. pIBA2-SpyTag-MBP

    Plasmid

    #107422

    Purpose

    Control construct for SpyCatcher experiments. Encodes MBP with N-terminal SpyTag, including OmpA signal peptide for periplasmic targeting.

    Depositor

    Jack Leo

    Article

    Chauhan et al Mol Microbiol. 2019 Jan 1. doi: 10.1111/

    Insert

    Maltose-binding protein (MalE)
    

    Use

    Tags

    OmpA signal peptide, SpyTag, and StrepII

    Expression

    Bacterial

    Mutation

    Promoter

    Tet

    Available Since

    Jan. 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pOpLexA::mini35s (GB0733)

    Plasmid

    #68180

    Purpose

    Provides the 4xLexA Operator fused to CaMV 35s minimal promoter as a level 0 GoldenBraid part

    Depositor

    Diego Orzaez

    Article

    Sarrion-Perdigones et al Plant Physiol. 2013 Jul;162(3):1618-31.

    Insert

    4xOpLexA:mini35S
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    BsaI and BsmBI sites removed

    Promoter

    Available Since

    Oct. 20, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pDGB1omega2_SF (GB0466)

    Plasmid

    #68243

    Purpose

    Twister plasmid to swap inserts from an omega1 or omega1R vector to any alpha level vector

    Depositor

    Diego Orzaez

    Article

    Sarrion-Perdigones et al Plant Physiol. 2013 Jul;162(3):1618-31.

    Insert

    Solanum lycopersicum intergenic region
    

    Use

    Synthetic Biology; Unspecified

    Tags

    Expression

    Mutation

    BsaI and BsmBI sites removed

    Promoter

    Available Since

    Oct. 27, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pDGB1alpha2_SF (GB0107)

    Plasmid

    #68233

    Purpose

    Twister plasmid to swap inserts from an alpha1 or alpha1R vector to any omega level vector

    Depositor

    Diego Orzaez

    Article

    Sarrion-Perdigones et al Plant Physiol. 2013 Jul;162(3):1618-31.

    Insert

    Solanum lycopersicum intergenic region
    

    Use

    Synthetic Biology; Unspecified

    Tags

    Expression

    Mutation

    BsaI and BsmBI sites removed

    Promoter

    Available Since

    Oct. 27, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pcDNA3.1/SPTBN1-FLT3

    Plasmid

    #168115

    Purpose

    Mammalian expression vector for the SPTBN1-FLT3 fusion gene identified in atypical chronic myeloid leukemia

    Depositor

    Nicholas Cross

    Article

    Grand et al Exp Hematol. 2007 Nov;35(11):1723-7. doi

    Insert

    SPTBN1-FLT3
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    April 30, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pLentiCRISPR sgSLC19A1

    Plasmid

    #102314

    Purpose

    genetic depletion of SLC19A1

    Depositor

    David Sabatini

    Article

    Kanarek et al Nature. 2018 Jul 11. pii: 10.1038/s41586

    Insert

    sgSLC19A1 (SLC19A1 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    U6

    Available Since

    July 13, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pTHsp18.2 (GB0035)

    Plasmid

    #68186

    Purpose

    Provides the Arabidopsis thaliana heat shock protein 18.2 terminator as a level 0 GoldenBraid part

    Depositor

    Diego Orzaez

    Article

    Sarrion-Perdigones et al Plant Physiol. 2013 Jul;162(3):1618-31.

    Insert

    AtHsp18.2 terminator
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    BsaI and BsmBI sites removed

    Promoter

    Available Since

    Oct. 21, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pET_RGG-RFP-RGG

    Plasmid

    #124944

    Purpose

    Exoression of RGG-RFP-RGG

    Depositor

    Benjamin Schuster, Matthew Good, Daniel Hammer

    Article

    Schuster et al Nat Commun. 2018 Jul 30;9(1):2985. doi:

    Insert

    RGG-RFP-RGG (using LAF-1 RGG domain) (laf-1 Nematode)
    

    Use

    Tags

    His and mCherry

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    May 29, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. Split-RESCUE-N

    Plasmid

    #170153

    Purpose

    AAV vector carrying the MCP-RESCUE-DDN (RESCUE is the evolved ADAR2 variant for C-U editing) with a nuclear export signal

    Depositor

    Prashant Mali

    Article

    Katrekar et al Elife. 2022 Jan 19;11. pii: 75555. doi:

    Insert

    NES-MCP-RESCUE-DDN
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Jan. 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pHH0103_NCK1-2/3

    Plasmid

    #91420

    Purpose

    Protein expression and purification of human SH3 domain construct NCK1-2/3

    Depositor

    Sachdev Sidhu

    Article

    Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

    Insert

    NCK1-2/3 (NCK1 Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    March 19, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pKDsgRNA-ldhA

    Plasmid

    #102288

    Purpose

    Contains arabinose-induced lambda Red and a tet-inducible gRNA targeting ldhA.

    Depositor

    Michael Lynch

    Article

    Moreb et al ACS Synth Biol. 2017 Oct 2. doi: 10.1021

    Insert

    ldhA gRNA
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    pTet

    Available Since

    April 12, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pPyCAG-NE-EGFP-IRES-Pac

    Plasmid

    #183617

    Purpose

    Mammalian expression vector: CAG promoter driving expression of nuclear envelope-tethered EGFP (NLS-EGFP-EmerinTMD). Confers puromycin resistance.

    Depositor

    Sally Lowell

    Article

    Malaguti et al Dev Cell. 2019 Jun 11. pii: S1534-5807(1

    Insert

    EGFP
    

    Use

    Tags

    Human EMD transmembrane domain and NLS

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available Since

    May 12, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. EK0545 SFFV-mCherry-SGc(GAPDH.s1)cSG-fs(EGFP)-(3xMS2) (FLP-IN)

    Plasmid

    #191177

    Purpose

    Mammalian expression of EK0493 with frameshift mutation (EGFP is frameshifted).

    Depositor

    Xiaojing Gao

    Article

    Kaseniit et al Nat Biotechnol. 2022 Oct 5. pii: 10.1038

    Insert

    mCherry:GAPDH.s1:EGFP (frameshifted)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    EGFP is frameshifted

    Promoter

    SFFV

    Available Since

    Oct. 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pHH0103_UBASH3A-1/1

    Plasmid

    #91478

    Purpose

    Protein expression and purification of human SH3 domain construct UBASH3A-1/1

    Depositor

    Sachdev Sidhu

    Article

    Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

    Insert

    UBASH3A-1/1 (UBASH3A Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    March 19, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge